Ex Vivo Equine Medial Tibial Plateau Contact Pressure With an Intact Medial Femoral Condyle,With a Medial Femoral Condylar Defect,and After Placement of a Transcondylar Screw Through the Condylar Defect          下载免费PDF全文

Alvaro G. Bonilla DVM  MS  Jarred M. Williams DVM  MS  PhD  Diplomate ACVS  Alan S. Litsky MD  ScD  Elizabeth M. Santschi DVM  Diplomate ACVS 《Veterinary surgery : VS》2015,44(3):289-296

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Effects of intravenous administration of tranexamic acid on hematological,hemostatic, and thromboelastographic analytes in healthy adult dogs          下载免费PDF全文

Efrat Kelmer DVM  MS  DACVECC  Gilad Segev DVM  DECVIM  Victoria Papashvilli  Naomi Rahimi‐Levene MD  MHA  Yaron Bruchim DVM  DACVECC  Itamar Aroch DVM  DECVIM  Sigal Klainbart DVM  DACVECC 《Journal of Veterinary Emergency and Critical Care》2015,25(4):495-501

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Femoral Fracture Repair Using a Locking Plate Technique in an Adult Captive Polar Bear (Ursus Maritimus)     

DAWN M. ZIMMERMAN DVM  MS  TERRY DEW DVM  MS  MICHAEL DOUGLASS DVM  EDWARD PEREZ MD 《Veterinary surgery : VS》2010,39(2):234-238

Objective— To report successful femoral fracture repair in a polar bear. Study Design— Case report. Animals— Female polar bear (Ursus maritimus) 5 years and ～250 kg. Methods— A closed, complete, comminuted fracture of the distal midshaft femur was successfully reduced and stabilized using a compression plating technique with 2 specialized human femur plates offering axial, rotational, and bending support, and allowing the bone to share loads with the implant. Postoperative radiographs were obtained at 11.5 weeks, 11 months, and 24 months. Results— Bone healing characterized by marked periosteal reaction was evident at 11 months with extensive remodeling evident at 24 months. No complications were noted. Conclusions— Distal mid shaft femoral fracture was reduced, stabilized, and healed in an adult polar bear with a locking plate technique using 2 plates. Clinical Relevance— Previously, femoral fractures in polar bears were considered irreparable. Use of 2 plates applied with a locking plate technique can result in successful fracture repair despite large body weight and inability to restrict postoperative activity.  相似文献   

Serum Starvation and Full Confluency for Cell Cycle Synchronization of Domestic Cat (Felis catus) Foetal Fibroblasts     

FRO de Barros  MD Goissis  HVA Caetano  FF Paula-Lopes  MA Peres  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2010,45(1):38-41

Nuclear transfer of domestic cat can be used as a tool to develop reproductive biotechnologies in wild felids. The importance of cell cycle phase during the nuclear transfer has been a matter of debate since the first mammalian clone was produced. The cell cycle phase of donor cells interferes on maintenance of correct ploidy and genetic reprogramming of the reconstructed embryo. The use of G0/G1 arrested donor cells has been shown to improve nuclear transfer efficiency. The present study was conducted to test the hypothesis that domestic cat foetal fibroblasts cultured up to the fifth passage and submitted to full confluency provide a higher percentage of cells at G0/G1 stage than fibroblasts cultured in serum starved media. Results demonstrated that serum starvation increased (p ≤ 0.05) the percentage of G0/G1 fibroblasts when compared with control. Moreover, the combined protocol using confluency and serum starvation was more efficient (p ≤ 0.05) synchronizing cells at G0/G1 stage than serum starvation or confluency alone for the first 3 days of treatment. In conclusion, serum starvation and full confluency act in a synergistic manner to improve domestic cat foetal fibroblast cell cycle synchronization at the G0/G1 stage.  相似文献   

Rht8、Rht10、Rht12矮杆基因对小麦营养生长和生殖生长发育的影响     

李杏普  兰素缺  张业伦  张京惠  冯延茹  刘书娥  Gale MD  Worland T J 《华北农学报》2009,24(Z1)

两年、两地的试验结果证明,Rht8半矮秆基因对地上部生物产量较Rht10和Rla12有显著的正向作用,并且生长发育较早,较接近目前推广种的生育时期,对不良环境有较强的适应性,利于产量性状的形成.Rht10的降秆作用最强,但由于节间缩短太多,造成地上部生物产量明显降低,使籽粒形成时提供营养的"源"太少,不利于高产.Rht12矮秆基因的降秆程度显著高于Rht8,但生物产量亦太低、成熟太晚和对环境条件的不适应,影响籽粒产量的提高,在以降秆为主要目标的育种中利用价值较大.  相似文献   

Evaluation of the onset of protection induced by a modified-live virus vaccine in calves challenge inoculated with type 1b bovine viral diarrhea virus     

Palomares RA  Givens MD  Wright JC  Walz PH  Brock KV 《American journal of veterinary research》2012,73(4):567-574

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Effect of phosphonoformic acid in the development of bovine embryos in vitro     

Marley MS  Givens MD  Stringfellow DA  Galik PK  Riddell KP 《Veterinary therapeutics : research in applied veterinary medicine》2006,7(2):156-166

This research evaluated the ability of phosphonoformic acid to inhibit bovine herpesvirus 1 (BHV-1) in cumulus cells commonly used in co-culture with bovine in vitro-produced embryos. At 200 and 400 microg/ml, phosphonoformic acid inhibited 4 logs of BHV-1. Subsequently, phosphonoformic acid (200 and 400 microg/ml) added to both in vitro fertilization and culture medium resulted in a decrease in the proportion of developed blastocysts, and the number of cells per blastocyst was lower in the treated embryos. Therefore, while phosphonoformic acid can effectively inhibit replication of BHV-1 in co-culture cells, it also inhibits development of in vitro-produced bovine embryos.  相似文献   

Effects of Sleep or Food Deprivation During Civilian Survival Training on Cognition, Blood Glucose and 3-OH-butyrate     

Lars Ståhle MD  PhD  Ewa Ljungdahl Ståhle PhD  Elisabeth Granström MD  PhD  Sven Isaksson PhD  Peter Annas PhD  Harry Sepp 《The Forestry Chronicle》2011,22(3):202-210

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Osteochondral Fragmentation in the Synovial Pad of the Fetlock in Warmblood Horses     

JEROEN DECLERCQ DVM  ANN MARTENS DVM  PhD  Diplomate ECVS  LIES BOGAERT DVM  PhD  BERNARD BOUSSAUW DVM  Diplomate ECVS  RAMSES FORSYTH MD  KARL JOSEF BOENING DVM  Diplomate ECVS 《Veterinary surgery : VS》2008,37(7):613-618

Objectives— To determine clinical and arthroscopic characteristics associated with fragments in the synovial pad of the fetlock and to characterize their morphology. Study Design— Retrospective study. Animals— Warmblood horses (n=104) with fragment(s) in the synovial pad. Methods— S ignalment and results of radiographic and clinical examination were collected before surgery. After arthroscopic fragment removal and joint evaluation for synovial and/or cartilage abnormalities, fragments were measured, and evaluated by histopathology. Results— Synovial pad fragments (n=142) were removed from 127 fetlocks. Two older horses had lameness. During arthroscopy, abnormalities were observed in 40 joints (31.5%) and multivariate logistic regression analysis showed a significant correlation between the observed arthroscopic abnormalities and the presence of large fragments (P=.016). Fragments were osteochondral bodies completely surrounded by fibrous tissue. At the edges of the hyaline cartilage cap an underlying fibrous structure was obvious in the extracellular matrix giving it a reactive pattern. Conclusions— Although the impact on lameness was minimal, there was a significant correlation between arthroscopic abnormalities and presence of large synovial pad fragments. On histopathology, these osteochondral fragments are embedded in fibrous tissue and show a reactive pattern. They are not a manifestation of any well‐described joint pathology. Clinical Relevance— Large synovial pad fragments in Warmblood horses can be associated with synovial and cartilage abnormalities, but further studies are warranted to determine their origin and clinical importance.  相似文献   

Video‐Assisted Thoracoscopic Resection of Noninvasive Thymomas Using One‐Lung Ventilation in Two Dogs     

PHILIPP D. MAYHEW BVM&S  Diplomate ACVS  JOSEPH S. FRIEDBERG MD  FACS 《Veterinary surgery : VS》2008,37(8):756-762

Objective— To report the technique and outcome of video‐assisted thoracoscopic surgery (VATS) for resection of cranial mediastinal thymoma in 2 dogs. Study Design— Case report. Animals— Eleven‐year‐old Labrador Retrievers (n=2). Methods— Two dogs had VATS resection of thymoma. Preoperative computed tomography (CT) scans revealed well‐circumscribed cranial mediastinal masses with dimensions of 4.5 cm × 4.2 cm × 3.7 cm and 2.1 cm × 2.1 cm × 4.1 cm (at the time of resection) without CT evidence of vascular invasion. One‐lung ventilation (OLV) was achieved using a bronchoscopically placed double‐lumen endobronchial tube. A 3‐portal technique was used for VATS access to the thorax. Thymomas were dissected from the tissues of the cranial mediastinum with the aid of a harmonic scalpel and retrieved in specimen retrieval bags. Results— Two cranial mediastinal thymomas were resected successfully, with their capsules intact, using a VATS technique. One dog developed aspiration pneumonia postoperatively which resolved with treatment and remains healthy 18 months postoperatively with no radiographic evidence of tumor recurrence. The second dog was diagnosed with myasthenia gravis, megaesophagus, and aspiration pneumonia preoperatively and despite recovering well from the procedure had a second episode of aspiration pneumonia 5 days postoperatively and was euthanatized. Conclusions— VATS resection of modestly sized noninvasive thymoma is possible in dogs. Careful case selection and preoperative imaging are the keys. Clinical Relevance— Thoracoscopic resection of modestly sized mediastinal masses forms part of an increasingly expansive set of indications for minimally invasive surgery in select veterinary patients.  相似文献