Effect of body mass index on severity and prevalence of varicocele     

Hassanzadeh K  Yavari-Kia P  Soleymanpour H  Ebrahimpour-Tolouei N  Alikhah H 《Pakistan journal of biological sciences: PJBS》2011,14(18):869-875

Varicocele is classified as grade I-III regarding its severity. This study was aimed to determine the correlation between height and weight with varicocele grade in 18-30 years age group. We enrolled 400 persons aged 18-30 years referred to the specialist's clinics of Tabriz Medical Sciences University or Medical Commission Since Sep. 2004 to Mar. 2005. First we divided the volunteers in two groups including Varicocele Group and Non-varicocele Group, then varicocele patients were classified to three grades considering the severity of the disease: severe (Grade III), moderate (Grade II) and mild (Grade I). Finally, the correlation between height, weight and Body Mass Index (BMI) was evaluated. There was a significant relation between height and grades of left-side varicocele; in other words the severity of disease was increased with height (p = 0.004). Also, height increased the prevalence ofvaricocele (p = 0.011). On the other hand, low weight and BMI increased the prevalence of varicocele (p = 0.000, p = 0.004) but did not affect the severity of disease (p = 0.364, p = 0.172). In conclusion, the height of patients directly affected the prevalence and severity of left-side varicocele which probably is related to length of left internal spermatic veins in these patients and increased hydrostatic pressure in taller patients. Also, the weight and BMI is effective on the prevalence of varicocele. It seems that slim and tall persons will benefit from evaluation while puberty.  相似文献   

Protective effects of crocin against cisplatin-induced acute renal failure and oxidative stress in rats     

Naghizadeh B  Boroushaki MT  Vahdati Mashhadian N  Mansouri MT 《Iranian Biomedical Journal》2008,12(2):93-100

Background. The major side effect of cisplatin, used in some tumours, is nephrotoxicity. Reactive oxygen species and oxidative damage are the most important factors in cisplatin-induced acute renal failure. The main purpose of this study is to investigate the protective effects of crocin against cisplatin-induced acute renal failure and oxidative stress in rat. Methods. In this study, animals were randomly divided into 5 groups (6 each). Group one received normal saline (2 ml/day, i.p.). Group two received a single dose of cisplatin (5 mg/kg, i.p.). Groups 3 to 5 received crocin (100, 200 and 400 mg/kg, i.p., respectively, for 4 consecutive days one hour before a single dose of cisplatin (5 mg/kg) only at the first day. Blood samples were taken out (on the fifth day) for measuring the level of urea and creatinine. The kidneys were removed for histopathological and biochemical examinations. Furthermore, 24-hour urinary factors were measured. Results. Blood urea, creatinine and urinary glucose and protein concentrations in crocin-treated groups were significantly lower than those of cisplatin-treated group in a dose-dependent manner. Histopathological studies showed a massive damage in S3 segment of proximal tubules in cisplatin-treated group. No damage was observed in crocin-treated groups. Crocin treatment resulted in a significant and dose-dependent reduction in malondialdehyde concentration as compared to the cisplatin-treated group. Moreover, crocin produced a significant elevation in total thiol and glutathione peroxidase concentrations, as compared with cisplatin-treated group. Conclusion. The results of the present study suggest that crocin has a protective effect against cisplatin-induced acute renal failure and relative oxidative stress.  相似文献   

Diagnosing feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infection: an update for clinicians     

ME Westman  R Malik  JM Norris 《Australian veterinary journal》2019,97(3):47-55

With the commercial release in Australia in 2004 of a vaccine against feline immunodeficiency virus (FIV; Fel‐O‐Vax FIV®), the landscape for FIV diagnostics shifted substantially. Point‐of‐care (PoC) antibody detection kits, which had been the mainstay for diagnosing FIV infection since the early 1990s, were no longer considered accurate to use in FIV‐vaccinated cats, because of the production of vaccine‐induced antibodies that were considered indistinguishable from those produced in natural FIV infections. Consequently, attention shifted to alternative diagnostic methods such as nucleic acid detection. However, over the past 5 years we have published a series of studies emphasising that FIV PoC test kits vary in their methodology, resulting in differing accuracy in FIV‐vaccinated cats. Importantly, we demonstrated that two commercially available FIV antibody test kits (Witness? and Anigen Rapid?) were able to accurately distinguish between FIV‐vaccinated and FIV‐infected cats, concluding that testing with either kit offers an alternative to PCR testing. This review summarises pertinent findings from our work published in a variety of peer‐reviewed research journals to inform veterinarians (particularly veterinarians in Australia, New Zealand and Japan, where the FIV vaccine is currently commercially available) about how the approach to the diagnosis of FIV infection has shifted. Included in this review is our work investigating the performance of three commercially available FIV PoC test kits in FIV‐vaccinated cats and our recommendations for the diagnosis of FIV infection; the effect of primary FIV vaccination (three FIV vaccines, 4 weeks apart) on PoC test kit performance; our recommendations regarding annual testing of FIV‐vaccinated cats to detect ‘vaccine breakthroughs’; and the potential off‐label use of saliva for the diagnosis of FIV infection using some FIV PoC test kits. We also investigated the accuracy of the same three brands of test kits for feline leukaemia virus (FeLV) diagnosis, using both blood and saliva as diagnostic specimens. Based on these results, we discuss our recommendations for confirmatory testing when veterinarians are presented with a positive FeLV PoC test kit result. Finally, we conclude with our results from the largest and most recent FIV and FeLV seroprevalence study conducted in Australia to date.  相似文献   

Interrelated effects of temperature, light and oxygen on Amaranthus caudatus L. seed germination     

Y. GUTTERMAN  F. CORBINEAU  D. CÔME 《Weed Research》1992,32(2):111-117

The aim of this study was to investigate the effects of temperature, light and oxygen on the germination of Amaranthus caudatus (var. viridis) seeds. At the highest temperature studied (35°C), seed germination was found to be most rapid and highest in both the dark and the light. Germination was inhibited by continuous white light. The lower the temperature, the greater the inhibitory effect of light. The far-red and blue spectral regions were the most effective in light inhibition. The light effect was also dependent on the percentage of oxygen in the atmosphere around the seeds; it was less marked in O₂-enriched atmospheres. Light/ oxygen dependency resulted from the action of the seed coat and endosperm. Scarified seeds were much less sensitive to light and hypoxia than non-scarified seeds. Effets combinés de la température, de la lumière et de I'oxygène sur la germination des graines d'Amaranthus caudatus L. Le but de ce travail était d'étudier les effets de la température, de la lumiere et de I'oxygène sur la germination des graines d'Amaranthus caudatus (var. viridis). A la temperature la plus élevée utilisée (35°C), la germination était la plus rapide et la plus complète tant à la lumière qu'à 1'obscuritè. La germination était inhibée par la lumière blanche continue. Plus la temperature était basse, plus 1'effet inhibiteur de la lumière était important. Les radiations rouge sombre et bleues du spectre étaient les plus in-hibitrices. L'effet de la lumière dépendait aussi de la teneur de 1'atmosphère en oxygène; il était moins marqué dans des atmosphères enrichies en oxygène. L'interaction lumière/oxygène provenait du tégument et de 1'albumen. Les graines scarifiées étaient beaucoup moins sensibles à la lumière et a 1'hypoxie que les graines non scarifiées. Wechselwirkungen von Temperatur, Licht und Sauerstoff auf die Keimung der Samen von Amaranthus caudatus L. Sowohl im Dunkeln als auch im Licht keimten die Samen von Amaranthus caudatus (var. viridis) bei 35°C, der höchsten Temperatur der Versuche, am schnellsten und meisten. Durch weiβes Dauerlicht wurde die Keimung gehemmt. Je niedriger die Temperatur, desto hölier die Hemmung durch Licht. Im entfernt roten und blauen Spektralbereich war die Lichthemmung am stärksten. Die Wirkung des Lichts hing auch vom Sauerstoffgehalt der die Samen umgebenden Luft ab: Bei O₂₅-Anreiche-rung war sie weniger deutlich. Die Licht/ Sauerstoff-Wechselwirkung wurde von der Samenschale und dem Endosperm beeinfluβt: Geritzte Samen waren gegenüber Licht und O₂-Mangel viel weniger empfindlich als ganze.  相似文献   

Dormancy of Bromus rubens L. seeds in relation to temperature, light and oxygen effects     

F. CORBINEAU  D. BELAID  D. CÔME 《Weed Research》1992,32(4):303-310

Freshly harvested mature Bromus rubens L. seeds are dormant. This dormancy corresponds to the inability of seeds to germinate in the dark at temperatures above 15°C, but is practically not apparent at temperatures between 5 and 15°C. Continuous white light markedly inhibits germination of dormant seeds, even at low irradiances, and reinforces their sensitivity to oxygen deprivation. However, prolonged illumination with white light does not alter subsequent germination in the dark. Breaking of dormancy during dry storage at 20°C results in a widening of the temperature range within which a high rate of germination occurs, and in almost complete disappearance of sensitivity of seeds to light. The structures surrounding the embryo (glumellae, caryopsis envelopes and possibly endosperm) are mainly responsible for dormancy and photosensitivity of whole seeds, probably because they limit the oxygen supply to the embryo. However, the embryo itself appears to play a role in dormancy. The results obtained are discussed in relation to dormancy of cereal seeds, and in relation to field establishment of B. rubens as a weed. La dormance des semences de Bromus rubens L. en relation avec les effets de la température, de la lumière et de l'oxygène Les semences mûres de Bromus rubens L. fraîchement récoltées sont dormantes. Leur dormance correspond à une inaptitude à germer à l'obscuritéà des températures supérieures à 15°C, mais elle ne se manifeste pratiquement pas entre 5 et 15°C. La lumière blanche continue inhibe nettement la germination des semences dormantes, même à de faibles énergies, et renforce leur sensibilitéà la privation d'oxygène. Cependant, un éclairement prolongé n'a aucune conséquence sur la germination ultérieure à l'obscurité. L'élimination de la dormance pendant la conservation au sec, à 20°C, se traduit par un élargissement de la gamme thermique qui assure une bonne germination et par la disparition presque complète de la sensibilité des semences à la lumière. Les structures qui entourent l'embryon (glumelles, enveloppes du caryopse et peut-être albumen) sont, en grande partie, responsables de la dormance et de la photosensibilité des semences entières, sans doute parce qu'elles limitent l'apport d'oxygène à l'embryon. Toutefois, l'embryon lui-même semble participer à la dormance. Les résultats obtenus sont discutés par comparaison avec la dormance des semences des céréales et en relation avec l'implantation, dans les cultures, de B. rubens en tant que mauvaise herbe. Dormanz der Samen von Bromus rubens L. in Bezug auf Temperatur, Licht und Sauerstoffversorgung Frisch geerntete Samen von Bromus rubens L. sind im Dunkeln bei Temperaturen über 15°C dormant, während diese Dormanz bei Temperaturen zwischen 5 und 15°C praktisch nicht vorliegt. Ständiges weißes Licht hemmt die Keimung dormanter Samen deutlich, sogar bei niedriger Intensität, und verstärkt ihre Empfindlichkeit gegenüber Sauerstoffmangel. Ständige Beleuchtung mit weißem Licht verändert jedoch nicht die folgende Dunkelkeimung. Das Brechen der Dormanz während der trockenen Lagerung bei 20 °C führt zu einem vergrößerten Temperaturbereich, in dem die Samen gut keimen, und läßt ihre Lichtempfindlichkeit fast vollständig verschwinden. Die Strukturen um den Embryo (Spelzen, Schale der Caryopse und möglicherweise Endosperm) sind hauptsächlich für die Dormanz und die Lichtempfindlichkeit des ganzen Samens verantwortlich, vermutlich deshalb, weil sie die Sauerstoffversorgung des Embryos einschränken. Aber auch der Embryo selbst scheint bei der Dormanz eine Rolle zu spielen. Die Untersuchungsergebnisse werden im Vergleich zur Dormanz von Getreidesamen und hinsichtlich des Vorkommens von Bromus rubens als Unkraut diskutiert.  相似文献   

Bioassay Procedure for the Evaluation of Erythromycin Activity in Aquaculture Environments     

Nasser A.  Assaf  Jairaj V.  Pothuluri  Rong-Fu  Wang Carl E.  Cerniglia Christine M.  Moffitt 《Journal of the World Aquaculture Society》1999,30(2):137-146

A new bioassay procedure was developed for the detection of erythromycin in aquaculture samples using a strain of a Stenotrophomonas as an indicator organism. Conventional disk-plate and well-plate radial diffusion assay procedures were developed, as well as a third procedure using the same indicator organism in Luria-Bertani (LB) broth, supplemented with the indicator dye Brilliant Black (40 μg/mL) in a multi-well microtiter plate. For both the disk-plate and well-plate radial diffusion assays, the response reflected in the size (width) of the growth inhibition zone, which was linear over the tested concentration range of 0.05 to 2.0 μg erythromycidml. The limit of quantitation of the bioassay was at 0.05 μg erythro-mycidml. Among the three methods of assay tested with Stenotrophomonas sp., the semi-quantitative dye reduction method is easy to read and is not diffusion dependent. This method allows for processing of more samples and more replication on a single titer plate. This new indicator organism is specific for erythromycin when tested in the presence of other antibacterial agents, i.e., oxytetracycline (Terramycin®) and/or Romet-Ma. This new bioassay procedure is suitable for quantitation of low concentrations of erythromycin in aquaculture water and sediment samples.  相似文献   

Expression of the μ Opioid Receptor and Effects of the Opioid Antagonist Naloxone on In Vitro Maturation of Oocytes Recovered from Anoestrous Bitches     

AI Iorga  L Valentini  T De Santis  B Ambruosi  M Albrizio  AC Guaricci  M Caira  ME Dell'Aquila 《Reproduction in domestic animals》2009,44(S2):263-268

The μ-opioid receptor (MOR) is expressed in bovine, human, equine and canine oocytes, and in seasonal breeders, it is expressed with higher intensity during the anoestrous phase. Supplementation of in vitro maturation (IVM) medium with opioid agents, agonists or antagonists, was shown to affect oocyte maturation in several species such as rat, bovine and equine. This study reports the effects of supplementing IVM medium with naloxone (Nx), an opioid antagonist, on nuclear and cytoplasmic maturation rate of oocytes recovered from anoestrous bitches. Cytoplasmic maturation was examined in terms of mitochondrial (mt) distribution. In order to confirm the receptor-mediated action of Nx, in oocytes of anoestrous bitches, MOR expression was analyzed by Western blot. Cumulus–oocyte complexes, recovered from the ovaries of bitches in anoestrous, were cultured in vitro and Nx was added at the concentrations of 1 × 10⁻⁶, 1 × 10⁻⁸ and 1 × 10⁻¹⁰  m . The rate of oocytes resuming meiosis after culture in presence of 1 × 10⁻⁶  m Nx (29%) was significantly higher than that of oocytes of control group (12%; p < 0.05). However, treatment with Nx did not affect mt distribution pattern. In denuded oocytes and in corresponding cumulus cells, a doublet of 65 and 50 kDa was observed. We conclude that, in oocytes of anoestrous bitches, MOR is expressed and Nx significantly improves nuclear maturation rate. Further studies should be performed to elucidate the expression of other opioid receptors, such as δ and κ, and possible interactive effects of their antagonists on canine oocyte maturation.  相似文献   

Acidification of a sandy grassland favours bacteria and disfavours fungal saprotrophs as estimated by fatty acid profiling     

Nasser Aliasgharzad  Linda-Maria Mårtensson 《Soil biology & biochemistry》2010,42(7):1058-1064

We have investigated the structure of a microbial community in semi-natural sandy grassland in southeast Sweden. The sand is rich in lime, but in most places the soil is decalcified in the upper layers, and therefore this site shows a large variation in pH within short distances. We collected samples at three different soil depths (0-10 cm, 10-20 cm and 20-30 cm) and found the pH to range from 5 to 8 in the topsoil and from 4.5 to 9.5 in the deepest layer. The abundance of saprophytic fungi and bacteria was investigated using signature phospholipid fatty acids and arbuscular mycorrhizal fungi (AMF) using the neutral lipid fatty acid 16:1ω5. The PLFA pattern of the topsoil was different from that in the other two layers, as indicated by principal component analysis. The saprotrophic fungi were associated with high pH, and bacteria with low pH in these sandy soils. No relation was found between pH and AMF in the topsoil, while a positive relation was found in the deepest soil layer. The saprophytic fungi-to-bacteria ratio was constant with depth, while the AMF-to-bacteria ratio increased with soil depth. The results showed that high soil pH favoured fungal saprophytes in sandy grasslands and that AMF are relatively more abundant than the other two groups in deeper soil layers; particularly so when the pH is high.  相似文献   

Lamb hemoglobinuria due to nutritional hypophosphatemia     

Alidadi N  Bazargani TT  Mashhadi AG 《The Canadian veterinary journal. La revue veterinaire canadienne》2005,46(10):947-948

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Second Oestrus Synchronization and Precocious Embryo Viability after Puberty Induction in Gilts by the Use of Gonadotrophin Treatment     

V do Lago  WL Vianna  RD Gama  A de  Campos Rosseto  ME Pinese  A de  Sant''Anna Moretti 《Reproduction in domestic animals》2005,40(2):141-144

The use of exogenous gonadotrophins in puberty inducement and ovulation synchronization is a technique that has a positive influence on the management of swine. The purpose of this study was to verify the effects of a second gonadotrophin treatment [equine chorionic gonadotrophin (eCG) and luteinizing hormone (LH), intramuscularly (i.m.)] upon the second oestrus synchronization and fertility in gilts. Seventy-one NAIMA (Pen Ar Lan) gilts had their first oestrus (puberty inducement) induced by a hormonal treatment (eCG and LH). Then, they were randomly distributed into two treatments, with (T1) and without (C) gonadotrophin treatment at the second oestrus. The animals were fed with a single ration (16% of crude protein and 3286.73 kcal ME/kg), and timed artificial insemination performed at the second oestrus. Gilts were slaughtered for embryo recovery and ovary examination about 5 days after insemination. There was no evidence of a difference in the percentage of the second oestrus expression (T1 - 90.90% and C - 86.84%), the duration of the oestrus cycle (T1 - 19.62 +/- 0.82 days and C - 19.67 +/- 4.14 days), the percentage of follicular cysts (T1 - 15.15% and C - 18.42%) and number of ovulations (T1 - 14.60 +/- 5.7 and C - 13.23 +/- 4.8) between treatments (p > 0.05). However, the hormonal treatment (T1) showed minor oestrus dispersion and embryo viability (T1 - 8.4 +/- 5.6 and C - 11.2 +/- 4.6) (p < 0.05). These results indicate that the better synchronization and expression of the second oestrus when using gonadotrophins (eCG and LH) is followed by a lower embryo viability, which is probably the consequence of the heterogeneous follicle recruitment during the injection of eCG.  相似文献