Immunolocalization of Melatonin and Follicle‐Stimulating Hormone Receptors in Caprine Ovaries and their Effects During in vitro Development of Isolated Pre‐Antral Follicles     

VRP Barros  AYP Cavalcante  TJS Macedo  RS Barberino  TLB Lins  BB Gouveia  VG Menezes  MAA Queiroz  VR Araújo  RC Palheta Jr  MCP Leite  MHT Matos 《Reproduction in domestic animals》2013,48(6):1025-1033

The expression of melatonin type 1 (MT1) and FSH (FSHR) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α‐MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR. Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles.  相似文献   

Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy     

C Klein  MHT Troedsson  J Rutllant 《Reproduction in domestic animals》2013,48(4):529-537

The expression of 12 different aquaporin subtypes in equine endometrium was examined at the mRNA and protein level. Endometrial samples were obtained during anoestrus, oestrus, 8, and 14 days after ovulation in non‐pregnant mares, and 14 days after ovulation in pregnant mares. Quantitative PCR revealed a time‐dependent pattern for all aquaporin subtypes examined except for AQP10 and 12. AQP3, 5 and 7 showed highest mRNA abundance 8 days after ovulation, while AQP0 and 2 were most abundant at Day 14 of the cycle in non‐pregnant mares. At 14 days of pregnancy, AQP1, 4, 8, 9 and 11 displayed highest expression levels. Western blot analysis confirmed protein expression of AQP0, 2 and 5. Immunohistochemistry localized protein expression to luminal and glandular epithelial and stromal cells. AQP0 staining intensity was highest in samples obtained on Day 14 of the oestrous cycle. AQP2 immunoreactivity seemed to be stronger in samples collected 14 days after ovulation from non‐pregnant animals, in particular luminal epithelial staining. Samples collected 8 days after ovulation from cyclic animals were characterized by intense AQP5 staining of glandular epithelium, predominantly in the deeper glands. Progesterone treatment of anoestrous mares did not enhance expression of AQPs, indicating that factors other than progesterone are required for the up‐regulation of certain AQP subtypes during dioestrus. In conclusion, it seems that an equine‐specific collaboration of aquaporin subtypes contributes to changes in endometrial fluid content occurring throughout the oestrous cycle and contributes to endometrial receptivity during early pregnancy in the mare.  相似文献   

Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells     

Ana P. Rodrigo  Vera M. Mendes  Bruno Manadas  Ana R. Grosso  Antnio P. Alves de Matos  Pedro V. Baptista  Pedro M. Costa  Alexandra R. Fernandes 《Marine drugs》2021,19(1)

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Antineoplastic activity of selected constituents of Duguetia glabriuscula     

Matos MF  Leite LI  Brustolim D  de Siqueira JM  Carollo CA  Hellmann AR  Pereira NF  da Silva DB 《Fitoterapia》2006,77(3):227-229

The cytotoxic effects of seven constituents isolated from Duguetia glabriuscula were evaluated against Hep2 human larynx carcinoma cells. The cytotoxicity exhibited by beta-sitosterol was as strong as that of cis-platin. (+)-Alloaromadendran-10,14beta-diol caused inhibition of cellular growth with IC50 values lower than 25 microg/ml, a feature that was considered as revealing significant activity. Polycarpol showed borderline cytotoxicity, whereas the other compounds were inactive.  相似文献   

Effects of pine processionary caterpillar Thaumetopoea pityocampa contact in dogs: 41 cases (2002-2006)     

Niza ME  Ferreira RL  Coimbra IV  Guerreiro HM  Félix NM  Matos JM  de Brito TV  Vilela CL 《Zoonoses and public health》2012,59(1):35-38

The pine processionary caterpillar, Thaumetopoea pityocampa, is considered an emerging pine pest in Mediterranean countries, with high medical relevance. In recent years, adverse reactions reports in humans following contact with T. pityocampa have been increasingly reported. Dogs living in pinewood areas are also frequently exposed to the caterpillar. This work consisted on a retrospective study of 41 cases of lepidopterism. All dogs presented drooling, dysphagia, submandibular lymphadenomegaly and clinical signs of pain. The animals were distributed in three groups, according to the time span from exposure to the caterpillar until presentation: up to 2 h (group 1), 2-5 h (group 2) and more than 5 h (group 3). All animals from groups 2 (n = 5) and 3 (n = 9), and eight dogs from group 1 (n = 27) developed lingual necrosis. Lepidopterism coursed through a predictable clinical pattern. The evolution was mainly dependent on the time span between exposure to the caterpillar and medical intervention, which should take place earlier than 2 h from exposure.  相似文献   

Effects of a deep container on morpho-functional characteristics and root colonization in Quercus suber L. seedlings for reforestation in Mediterranean climate     

E. Chirino  A. Vilagrosa  E.I. Hernndez  A. Matos  V.R. Vallejo 《Forest Ecology and Management》2008,256(4):779-785

In the last decades, reforestation and afforestation programs are being carried out mainly with containerized seedlings. Container design determines the morphological and physiological characteristics of seedlings. However, container characteristics are often the same for plant species with very different growth strategies. The most commonly used nursery containers are relatively shallow and limit tap root growth; consequently, species relying on the early development of a long tap root to escape drought, such as those of the Quercus genus, might need to be cultivated in deep containers. The aim of this paper was to compare the morphological and physiological characteristics of Quercus suber L. seedlings cultivated in shallow containers (CCS-18, depth 18 cm) with seedlings cultivated in deep containers (CCL-30, depth 30 cm). Both container types used were made of high-density polyethylene, cylindrical in shape, open-bottomed, with a diameter of 5 cm, two kinds of vertical ribs on the inside wall showing a cultivation density of 318 seedlings/m². At the end of nursery culture, the seedlings cultivated in the CCL-30 deep container presented a longer tap root, higher shoot and root biomass and higher Dickson Quality Index (DQI). Moreover, the CCL-30 seedlings showed a higher root growth capacity (RGC), they reached deep substrate layers faster and they presented higher root hydraulic conductance. These morpho-functional advantages improved the CCL-30 seedling water status, which was expressed by higher stomatal conductance during an imposed drought period.  相似文献   

The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating‐induced endometritis          下载免费PDF全文

CE Fedorka  KE Scoggin  EM Woodward  EL Squires  BA Ball  MHT Troedsson 《Reproduction in domestic animals》2017,52(1):89-96

In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating‐induced endometritis PMIE Select seminal plasma proteins cysteine‐rich secretory protein‐3 (CRISP‐3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP‐3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Six mares classified as susceptible to PMIE were inseminated during four consecutive oestrous cycles with treatments in randomized order of: 1 mg/ml CRISP‐3, 150 μg/ml lactoferrin, seminal plasma (positive control) or lactated Ringer's solution (LRS; negative control) to a total volume of 10 ml combined with 1 × 10⁹ spermatozoa pooled from two stallions. Six hours after treatment, an endometrial biopsy was obtained for qPCR analysis of selected genes associated with inflammation (pro‐inflammatory cytokines interleukin (IL)‐1β, IL‐8, tumour necrosis factor (TNF)‐α, interferon (INF)‐γ, anti‐inflammatory cytokines IL‐1RN and IL‐10, and inflammatory‐modulating cytokine IL‐6). Seminal plasma treatment increased the mRNA expression of IL‐1β (p = .019) and IL‐8 (p = .0068), while suppressing the mRNA expression of TNF (p = .0013). Lactoferrin also suppressed the mRNA expression of TNF (p = .0013). In conclusion, exogenous lactoferrin may be considered as one modulator of the complex series of events resulting in the poorly regulated pro‐inflammatory response seen in susceptible mares.  相似文献   

Chemical Composition and Starch Digestibility of Different Gluten-free Breads     

María Estela Matos Segura  Cristina M. Rosell 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(3):224-230

The increasing demand for gluten-free products has favoured the design of numerous gluten-free bakery products which intended to mimic the quality characteristics of wheat bakery products. The objective of this study was to evaluate the nutritional pattern of gluten-free breads representative of the Spanish market for this type of products. The protein, fat and mineral content of the gluten-free breads showed great variation, ranging from 0.90 to 15.5 g/100 g, 2.00 to 26.1 g/100 g and 1.10 to 5.43 g/100 g, respectively. Gluten-free breads had very low contribution to the recommended daily protein intake, with a high contribution to the carbohydrate dietary reference intake. Dietary fiber content also showed great variation varying from 1.30 to 7.20 g/100 g. In vitro enzymatic hydrolysis of starch showed that the most predominant fraction was the rapidly digestible starch that varied from 75.6 to 92.5 g/100 g. Overall, gluten-free breads showed great variation in the nutrient composition, being starchy based foods low in proteins and high in fat content, with high glycaemic index.  相似文献   

The use of mobile devices with multi-tag technologies for an overall contextualized vineyard management     

Carlos R. Cunha Author Vitae  Emanuel Peres Author Vitae  Raul Morais Author Vitae  Ana A. Oliveira Author Vitae  Samuel G. Matos Author Vitae  Miguel A. Fernandes Author Vitae  P.J.S.G. Ferreira Author Vitae Author Vitae 《Computers and Electronics in Agriculture》2010,73(2):154-164

This paper describes a Viticulture Service-Oriented Framework (VSOF) which turns around context elements or tags that are placed in the field and which can be decoded by mobile devices such as mobile phones or PDAs. The tags are used to automatically associate a field location to the relevant database tables or records and also to access contextual information or services. By pointing a mobile device to a tag, the viticulturalist may download data such as climatic data or upload information such as disease and pest incidence in a simple way, without having to provide coordinates or any other references, and without having to return to a central office. This work is part of an effort to implement a large-scale distributed cooperative network in the Douro Demarcated Region in Northeast Portugal, a region in which the effort makes particular sense due to the extremely variable topography and mesoclimates. The possibility of exchanging contextualized information and accessing contextualized services in the field, using well-known devices such as cell phones, may contribute to increase the rate of adoption of information technology in viticulture, and contribute to more efficient and closer-to-the-crops practices.  相似文献   

Phytotoxity of Fusarium subglutinans culture filtrates on in vitro plantlets and calli of resistant and susceptible pineapple (Ananas comosus)     

Hidalgo  Santos  Tussel  Pires de Matos  Cabral  Arzola  & Pérez 《Plant pathology》1999,48(6):756-758

Various concentrations of culture filtrates from Fusarium subglutinans were tested for their effect on in vitro multiplication of plantlets and regeneration from calli of pineapple. The cultivars tested were the resistant Perolera and Spring and the susceptible Perola and Smooth Cayenne. The culture filtrate reduced shoot formation of in vitro plantlets and regeneration of plants from calli derived from the susceptible cultivars but plantlets and calli from resistant plants showed resistance. These results show the potential for culture filtrates of F. subglutinans to provide a method of in vitro screening of pineapple for resistance to the pathogen.  相似文献