In vitro effects of glucosamine and acetylsalicylate on canine chondrocytes in three-dimensional culture     

Anderson CC  Cook JL  Kreeger JM  Tomlinson JL  Wagner-Mann CC 《American journal of veterinary research》1999,60(12):1546-1551

OBJECTIVE: To determine effects of glucosamine and acetylsalicylate on canine chondrocytes in 3-dimensional culture. SAMPLE POPULATION: Chondrocytes isolated from articular cartilage of 2 adult female dogs recently euthanatized for reasons unrelated to orthopedic abnormalities. PROCEDURE: Chondrocytes were cultured in a 3-dimensional agarose-based medium alone (control), with glucosamine (100 microg/ml; GL), or with acetylsalicylate (18 microg/ml; AS). Supernatant and agarose plugs from 4 wells/group/d were collected on days 3, 6, and 12 of culture. Agarose plugs were evaluated for percentage of viable cells, percentage of cells producing pericellular or territorial matrix, glycosaminoglycan (GAG) concentration, and type-II collagen production. Prostaglandin E2 concentration in supernatants was determined. RESULTS: Chondrocytes in all groups had characteristics indicative of viability and differentiation; however, on day 12, a lower percentage of viable cells was detected in the AS group, compared with the other 2 groups. On day 6, GAG concentration in the AS group was significantly greater than concentrations in the other 2 groups. On day 12, GAG concentrations in the GL and AS groups were significantly less than in the control group. Within the GL and AS groups, cell viability was significantly less on day 12, compared with day 3. Significant differences in PGE2 concentration among or within groups and evidence of type II collagen production were not detected. CONCLUSIONS: 3-dimensional culture of canine chondrocytes allows for production of hyaline cartilage matrix constituents and growth of cells with morphologic characteristics similar to those of articular cartilage. Acetylsalicylate and glucosamine, at the single concentration evaluated, had detrimental effects on chondrocyte viability, GAG production, or both.  相似文献   

Effects of percutaneous liver biopsy in alpacas and llamas     

Anderson DE  Silveira F 《American journal of veterinary research》1999,60(11):1423-1425

OBJECTIVE: To determine changes in results of selected serum biochemical tests after a single percutaneous liver biopsy and changes in results of hematologic and selected serum biochemical tests after multiple percutaneous liver biopsies in alpacas and llamas. ANIMALS: 5 llamas and 10 alpacas. PROCEDURE: A single percutaneous liver biopsy was performed in 5 llamas and 6 alpacas. Blood samples were collected before and 1, 2, and 3 days after liver biopsy, and submitted for serum biochemical analysis. In the other 4 alpacas, liver biopsy was performed on day 0 and then weekly for 4 weeks. Blood samples were collected at the time of each biopsy, and CBC and serum biochemical analyses were performed. Attitude and appetite of all animals were evaluated daily. RESULTS: None of the animals developed clinically apparent adverse effects. A mild decrease in sorbitol dehydrogenase activity was detected in animals that underwent a single liver biopsy, and mild decreases in plasma protein and albumin concentrations were detected in animals that underwent multiple biopsies. Other significant changes were not detected. CONCLUSIONS: Results of this study suggest that liver biopsy is safe in healthy llamas and alpacas and that, if necessary, multiple weekly liver biopsies can be performed safely in healthy alpacas.  相似文献   

Prevalence of Campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system     

Harvey RB  Young CR  Ziprin RL  Hume ME  Genovese KJ  Anderson RC  Droleskey RE  Stanker LH  Nisbet DJ 《Journal of the American Veterinary Medical Association》1999,215(11):1601-1604

OBJECTIVE: To enumerate the prevalence of Campylobacter isolates in the intestinal tract of market-weight swine raised in an integrated swine operation in Texas. SAMPLE POPULATION: Samples of cecal contents were collected from 595 pigs (mean body weight, 110 kg [242 lb]) at time of slaughter. Pigs were off-spring of Yorkshire-Landrace sows and Duroc or Hampshire boars. Pigs originated from 4 farrow-to-finish farms. PROCEDURE: During a 9-month period, visits were made to a slaughter plant to remove cecal contents from market-weight hogs. Samples were obtained from 50 pigs/visit from designated farms so that samples were obtained 3 times from pigs of each of 4 farms. Isolation of Campylobacter spp was accomplished by use of enrichment broth and restrictive media, using microaerophilic conditions. RESULTS: Campylobacter spp were isolated from 70 to 100% of the pigs, depending on the farm and the date the samples were collected. Campylobacter coli was isolated from 20 to 100% (mean, 60%) of samples, and C jejuni was isolated from 0 to 76% (mean, 31%) of samples. Campylobacter lari was isolated from 2 pigs. Concentrations of C coli or C jejuni ranged from 10(3) to 10(7) colony-forming units/g of cecal content. CONCLUSIONS AND CLINICAL RELEVANCE: Campylobacter coli generally is accepted as a common inhabitant of the intestinal tract of swine. However, analysis of results of this study suggests that a relatively high prevalence of C jejuni may be found in pigs raised on specific farms.  相似文献   

Treatment of lantana poisoning in cattle     

MW McLENNAN  ML AMOS 《Australian veterinary journal》1989,66(3):93-94

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Borreliosis in equids in northeastern United States   总被引：3，自引：0，他引：3  

L A Magnarelli  J F Anderson  E Shaw  J E Post  F C Palka 《American journal of veterinary research》1988,49(3):359-362

During 1982 and 1985, blood samples from 705 equids were examined for antibodies to Borrelia burgdorferi. By indirect immunofluorescence staining, IgM and total immunoglobulin (IgM and IgG) antibodies were detected in 37 (5.3%) and 90 (12.8%) serum specimens, respectively. The geometric mean titer for IgM antibody (140.4) was highest during July, whereas total immunoglobulin ranged from 94.1 in October to 338 in May. Eighty-six equids with total immunoglobulin to B burgdorferi lived in areas of Connecticut where the primary tick vector, Ixodes dammini, was present. Of the 86 equids, 9 from Lyme, Connecticut and Westchester County, New York had antibodies to B burgdorferi and developed limb or joint disorders that resulted in single or recurrent episodes of lameness.  相似文献   

Induction of parturition in cattle with corticosteroids: an analysis of field trials   总被引：2，自引：0，他引：2  

R A Welch  P Newling  D Anderson 《New Zealand veterinary journal》1973,21(6):103-108

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Observations on mouse mastitis produced by inoculation of strains of staphylococcus isolated from herds of different susceptibility to cloxacillin therapy     

J C Anderson 《The British veterinary journal》1972,128(11):65-68

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A comparison of haemoglobins from the adult and newborn dog     

C K Lee  G V Odell  I L Anderson  F P Eliot  E W Jones 《Research in veterinary science》1973,15(3):333-337

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Prevention and treatment of canine hookworm disease with styrylpyridinium and diethylcarbamazine     

H W Casey  G S Tulloch  R A Anderson 《Journal of the American Veterinary Medical Association》1971,159(8):1003-1007

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Immune response of pigs to inactivated foot-and-mouth disease vaccines. Response to emulsion vaccines   总被引：2，自引：0，他引：2  

E C Anderson  R C Masters  G N Mowat 《Research in veterinary science》1971,12(4):342-350

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