Effect of mitochondrial uncoupling and glycolysis inhibition on ram sperm functionality          下载免费PDF全文

JDA Losano  DSR Angrimani  A Dalmazzo  BR Rui  MM Brito  CM Mendes  GKV Kawai  CI Vannucchi  MEOA Assumpção  VH Barnabe  M Nichi 《Reproduction in domestic animals》2017,52(2):289-297

Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram sperm in response to mitochondrial uncoupling and glycolysis inhibition aiming to assess the importance of each pathway for sperm functionality. Statistical analysis was performed by the SAS System for Windows, using the General Linear Model Procedure. Data were tested for residue normality and variance homogeneity. A p < .05 was considered significant. Groups treated with the mitochondrial uncoupler Carbonyl cyanide 3 chlorophenylhydrazone (CCCP) showed a decrease in the percentage of cells with low mitochondrial activity and high mitochondrial membrane potential. We also observed that the highest CCCP concentration promotes a decrease in sperm susceptibility to lipid peroxidation. Regardless the lack of effect of CCCP on total motility, this substance induced significant alterations on sperm kinetics. Besides the interference of CCCP on spermatic movement patterns, it was also possible to observe such an effect in samples treated with the inhibitor of glycolysis (2‐deoxy‐d ‐glucose, DOG). Furthermore, treatment with DOG also led to a dose‐dependent increase in sperm susceptibility to lipid peroxidation. Based on our results, we suggest that the glycolysis appears to be as important as oxidative phosphorylation for ovine sperm kinetics as this mechanism is capable of maintaining full motility when most of the cells have a low mitochondrial membrane potential. Furthermore, we found that changes in the glycolytic pathway trough glycolysis inhibition are likely involved in mitochondrial dysfunction and sperm oxidative unbalance.  相似文献   

Antibody Prevalence and Risk Factors for Toxoplasma gondii Infection in Women from Multan,Pakistan          下载免费PDF全文

M. M. Nazir  M. Akhtar  A. Maqbool  A. Waheed  M. A. Sajid  M. A. Ali  M. Oneeb  M. A. Alam  A. N. Ahmad  N. Nazir  S. Fatima  D. S. Lindsay 《Zoonoses and public health》2017,64(7):537-542

Toxoplasma gondii infections are prevalent in humans and warm‐blooded animals. Maternal infections during pregnancy may have devastating consequences for transplacentally infected neonates. This study was conducted to examine the seroprevalence of antibodies to T. gondii in pregnant women of childbearing age and determine risk factors associated with pregnancy history, pet ownership, social and cultural factors at Nishtar Hospital, Multan. Samples were collected from 403 women and examined using a commercially available enzyme‐linked immunosorbent assay (ELISA). The overall prevalence of antibodies to T. gondii was 17.6% (71) in the 403 samples collected from women. Antibodies to T. gondii were present in 19.4% (45) of 232 pregnant women and 15.2% (26) of the samples from 171 non‐pregnant women. This study identified miscarriage history, pet ownership, type of residence, marital status, source of drinking water and eating habits as significant (P < 0.05) risk factors associated with the presence of antibodies to T. gondii infection. Seroprevalence was not significantly different (P > 0.05) in women from different ethnic groups based upon lifestyle and culture.  相似文献   

Haemolytic anaemia in cattle in NSW associated with Theileria infections     

MM Izzo  I Poe  N Horadagoda  AJ De Vos  JK House 《Australian veterinary journal》2010,88(1-2):45-51

Several outbreaks of anaemia, jaundice, abortion and mortality in cattle in New South Wales were attributed to the intracellular parasite, Theileria buffeli . Disease occurred predominantly in periparturient animals that had been moved from inland to coastal areas. Diagnosis was made via exclusion of other causes of haemolytic anaemia and observation of the parasite in blood smears. Treatments included both registered and non-registered products. There is a possibility of a new strain of Theileria sp. in Australia and the possible vectors encountered in NSW are discussed.  相似文献   

Effect of Luteinizing Hormone and Tumour Necrosis Factor‐Alpha on VEGF Secretion by Cultured Porcine Endometrial Stromal Cells     

MM Kaczmarek  A Blitek  D Schams  AJ Ziecik 《Reproduction in domestic animals》2010,45(3):481-486

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Effect of stale seedbed preparations and subsequent weed control in lettuce (cv. Iceboll) on weed densities     

MM RIEMENS  RY VAN DER WEIDE†  PO BLEEKER†  & LAP LOTZ 《Weed Research》2007,47(2):149-156

The effects of stale seedbed preparations and several weed control methods on the emergence of weeds in lettuce were studied. The specific goal was to evaluate the use of a stale seedbed in combination with chemical or mechanical weed control methods in the field. Depending on location and year, stale seedbed preparations followed by weed control prior to planting reduced the amount of weeds during crop growth by 43–83%. Control of the emerged seedlings after a stale seedbed preparation was more effective with glyphosate than with a rotary harrow. Covering the rotary harrow during control to prevent light reaching the soil improved its effect on the weed density during crop growth in two of 3 years. Radiation with far red light (FR) did not reduce the number of emerging weeds in this study. Mechanical control by finger weeder, torsion weeder and hoe was applied without stale seedbed preparations. These measures reduced the weed densities by 88–99%, compared with the untreated control and were more effective than chemical weed control with carbetamide and chlorpropham. The results show that the stale seedbed technique in combination with mechanical control of emerging weeds can reduce the weed population during crop growth as effectively as chemical control. The technique may therefore help reduce the use of herbicides in lettuce crops in the future.  相似文献   

Thoracic haemangiosarcoma in a 3-year-old horse     

JF FREESTONE  MM WILLIAMS  G NORWOOD 《Australian veterinary journal》1990,67(7):269-270

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Inhibition of Boar Sperm Binding to Homologous Zona Pellucida by Antibodies against ZP3α and ZP3β Glycoproteins     

N Blase  AR Fazeli  E Topper  MM Bevers  H Woelders  E Töpfer-Petersen  B Colenbrander 《Reproduction in domestic animals》1998,33(1):21-25

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Genetic analysis of post-weaning growth traits of Thalli sheep under tropical conditions     

Asghar Hussain  Pervez Akhtar  Safdar Ali  Khalid Javed  Muhammad Younas  Abdul Shakoor  Usman Waheed 《Tropical animal health and production》2014,46(8):1527-1531

Present investigation was carried out to study the influence of genetic and non-genetic factors affecting post-weaning performance traits in Thalli sheep. Data on post-weaning growth of Thalli sheep maintained at Livestock Experiment Station, Rakh Ghulaman, District Bhakkar-Punjab, Pakistan during 1977–2003 were subjected to genetic analysis. The average values for weight at 180 and 270 days of age, yearling weight and post-weaning average daily gain were 22.37?±?4.21, 25.96?±?4.90 and 28.93?±?5.20 kg and 28?±?0.01 g/day, respectively. Weight at 180, 270 and 365 days of age and post-weaning average daily gain were significantly affected by year of birth, sex, type of birth and weaning weight (covariable) whereas season of birth was a significant source of variation for 180 and 270 days of age. The heritability estimates for 180 and 270 days, yearling weight and post-weaning average daily gain were 0.07?±?0.02, 0.08?±?0.02, 0.07?±?0.02 and 0.07?±?0.02, respectively.  相似文献   

Effects of Exogenous Tumour Necrosis Factor-α on the Secretory Function of the Bovine Reproductive Tract Depend on Tumour Necrosis Factor-α Concentrations     

DJ Skarzynski  KK Piotrowska  MM Bah  A Korzekwa  I Woclawek-Potocka  K Sawai  K Okuda 《Reproduction in domestic animals》2009,44(3):371-379

The aim of study was to correlate tumour necrosis factor-α (TNF) infused doses used with the TNF concentrations achieved and with the secretory function of both the ovary and the uterus in cows. We evaluated the concentrations of progesterone (P4), prostaglandin (PG)F_2α, PGE₂ nitric oxide (NO) and TNF in the jugular vein and vena cava caudalis as parameters of exogenous TNF action on the female reproductive tract. Aortae abdominalis of cows (n = 18) were infused with saline or two doses of TNF (luteolytic – 1 μg or luteotrophic – 10 μg). In the peripheral blood, 1 μg TNF concentrations achieved within the range of 30–45 pg/ml, and 10 μg TNF provoked a sharp increase in achieved concentrations at a range of 250–450 pg/mL). The TNF concentrations achieved in vena cava caudalis were five to six times higher than that in peripheral blood (p < 0.001). One microgram TNF increased PGF_2α and NO (p < 0.001) and decreased P4 (p < 0.05). The higher TNF dose stimulated P4 and PGE₂ (p < 0.01). TNF infusion at luteolytic dose achieved its concentrations at the physiological range previously observed in cows. Luteotrophic TNF dose achieved the concentrations in vena cava caudalis that are much higher than physiological level and were previously noted in pathological circumstances (i.e. mastitis , metritis ).  相似文献   

Avian influenza virus H9 subtype in poultry flocks in Jordan     

Roussan DA  Khawaldeh GY  Al Rifai RH  Totanji WS  Shaheen IA 《Preventive veterinary medicine》2009,88(1):77-81

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