不同栽植密度对辣椒产量的影响研究     

肖莉  郑元红  牟东岭  毛诗友  马西艳  胡显钊  王慧 《现代农业科技》2011,(2):119-121

为进一步提高毕节地区名优辣椒的种植水平,开展不同栽植密度对金椒1号和黔西泡通椒产量影响的研究试验,结果表明:金椒1号、黔西泡通椒栽植密度分别为8 596、8 748株/666.67 m2时,最高产量分别可达225.55、248.17 kg/667.67 m2,经济效益最好。  相似文献   

花溪灰萝卜区域试验研究   总被引：2，自引：0，他引：2  

牟琼  王应芬  杨义成  吴佳海  周玉锋  吴有松  宋世伦 《草业与畜牧》2013,(2)

为筛选出具有推广潜力、产量较高、抗逆性强并适应贵州气候条件的饲用灰萝卜新品种,对3个灰萝卜品种的生育期、生产性能、抗逆性等进行了对比分析,结果表明,花溪灰萝卜品种优势突出,表现为产量高、抗逆性强,具有较大的推广潜力.  相似文献   

TGF β₁ inhibits the maturation of dendritic cells and down-regulates TLR4 expression     

MOU Hai-bo  LIN Mao-fang  CEN Hong  HUANG He 《园艺学报》2005,21(8):1476-1479

AIM: To investigate the effects of transforming growth factor β1 (TGF-β1) on murine-derived dendritic cells (DC). METHODS: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF β-DC. Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method. IL-12 p70 protein was detected by ELISA and the expressions of Toll like receptor 4 (TLR4) on DCs were measured by semi-quantitative RT-PCR and FCM. RESULTS: Compared to immature DC (imDC) cultured with GM-CSF alone, the expressions of CD80, CD86, I-Ab and CD40 in TGF β-DC were lower. The TGF β-DC was resistant to maturation by LPS. Maturation resistance was evident from a failure to up-regulate CMs, to stimulate larger T cell proliferation and to increase secretion of IL-12 p70. Down-regulation of TLR4 expression on TGF β-DC was also found. CONCLUSION: TGF-β1 inhibits the expression of co-stimulatory molecules on DC. It is resistant to maturation stimulus (LPS) and might be linked with TLR4 down-regulation.  相似文献   

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide     

LI Hong  XIAO Ying-bin  YANG Tian-de  MOU Zhi-rong  ZOU Li-yun  HUANG He 《园艺学报》2007,23(5):844-847

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.  相似文献   

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys     

MOU Bo  YANG Zhi-hong  ZHAO Jia-wei  WANG Xuan-chun  DONG Xue-hong  LIU Yu  HU Ren-ming 《园艺学报》2007,23(5):888-892

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in β-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were β cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of β cell damage in diabetes probably through the mechanism of energy metabolism disturbance.  相似文献   

双向电泳结合蛋白质印迹技术分析猪囊尾蚴乳酸脱氢酶     

方文  包怀恩  肖靓靓  牟荣 《黑龙江畜牧兽医》2011,(15)

为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。  相似文献   

草菇床栽料面保湿对菌丝生长和生物效率的影响   总被引：1，自引：0，他引：1  

纪开萍  何明霞  王文兵 《热带农业科技》2006,29(4):10-12

在西双版纳6～7月,对草菇菇房空间及菇床料面的喷雾温水保湿试验结果:喷30～45℃温水可保持料面湿润,促进培菌期草菇菌丝生长,促进出菇,不影响幼菇蕾生长,喷温水后菇产量为对照(菇床料面不喷温水)的280%～332%。  相似文献   

木本能源植物文冠果的表型多样性研究   总被引：8，自引：2，他引：8       下载免费PDF全文

牟洪香  侯新村  刘巧哲 《林业科学研究》2007,20(3):350-355

以文冠果为研究对象,调查了14个分布区文冠果的15个表型性状,利用变异系数和巢式方差分析研究了分布区间和分布区内的变异情况;应用相关分析揭示了表型性状间及其与地理因子间的关系;运用聚类分析进行了分布区分类.结果表明,文冠果分布区内的变异大于分布区间的变异,分布区间的分化相对较小;出种数随经度的增大而减少,果宽随纬度的增大而增大,但随年平均气温的增大而减小,而其它13个性状随地理位置的改变没有特别明显的变化;表型性状的欧式距离与地理距离相关不显著.  相似文献   

野生细鳞鱼的生物学特性及繁殖力   总被引：1，自引：0，他引：1  

白庆利  牟振波  于洪贤  尹家胜  贾钟贺 《水产学杂志》2007,20(1):69-73

本文对采捕的野生细鳞鱼的生物学特性及繁殖力进行了测定,结果表明:细鳞鱼可量比例性状为:全长/体长1.13±0.02,体长/体高4.86±0.30,体长/头长4.41±0.19,体长/尾柄长6.94±0.72,体长/尾柄高12.53±0.59,尾柄长/尾柄高1.82±0.17,头长/吻长3.36±0.31,头长/眼径5.41±0.52,头长/眼间距2.89±0.26;可数性状为:背鳍鳍式:II,11～13,胸鳍鳍式:I,15～19,腹鳍鳍式:I,9～12,臀鳍鳍式:II,10～14,侧线鳞:108～163,侧线上鳞:24～37,侧线下鳞:23～36,第一鳃弓鳃耙数:15～29,脊椎骨:57～61,肋骨:28～31,幽门垂:70～121。绝对繁殖力2823.25±1237.01,相对繁殖力3.20±1.79。  相似文献   

以生态恢复为导向的郪江流域大英段植物景观规划     

冯美文  牟江 《林业调查规划》2014,(2):92-97

流域植被作为基础的自然"本底",在构建和维护河流生态系统稳定方面有着重要作用。通过对生态恢复定义及模式的探析,以流域植物为主要分析对象,浅析其界定与特征。以郪江流域大英段生态恢复项目为载体,展开流域植物景观规划研究,阐述总体策略引导下的植物评价及规划。  相似文献