Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir     

Sandra Escribano  Almudena Lázaro  Hugo E. Cuevas  Ana I. López-Sesé  Jack E. Staub 《Genetic Resources and Crop Evolution》2012,59(3):359-373

Melon (Cucumis melo L.) landraces of the Madrid provenance, Spain, have received national distinction for their high fruit quality and sensorial attributes. More specifically, a unique array of Group Inodorus landraces have been continuously cultivated and conserved by farmers in the municipality of Villaconejos since the 19th century. Their genetic relationships to other Group Inodorus and Flexuous melon market classes is not known, and, thus, a study was designed to determine their genetic relationships using 52 simple sequence repeat (SSR) markers, and then make genetic comparisons between these accessions and a previously published “Standard Reference Germplasm Array” (RA) containing Group Inodorus (14 Spanish and one USA), Flexuosus (1 Spanish), and Cantalupensis ( 2 USA) melon accessions. This subset consisted of 15 Spanish Group Inodorus landraces that circumscribed the genetic variation of major Spanish melon market classes (Groups Inodorus and Flexuosus), and USA commercial varieties (Groups Cantalupensis and Inodorus). Based on genetic distances, Villaconejos (Madrid) genotypes differed substantially from RA subset accessions, thus defining their genetic uniqueness. Principal component analysis (PCA) partitioned the accessions examined into four distinct groups revealing that Villaconejos black epidermis melons (landraces ‘Largo’, ‘Largo Negro Escrito’ and ‘Puchero’) were distinctly different from all other accessions examined, as cluster analysis separated Rochet market type Villaconejos’ accessions (landraces ‘Mochuelo’, ‘Mochuelo Tradicional’ and ‘Melón de Villaconejos’) from RA of the same market type. Genetic assessment of principal Spanish market classes revealed comparatively low intra-market heterogeneity in Piel de Sapo type accessions and high heterogeneity in Black and Yellow market type accessions. While a relatively high level of genetic introgression was detected between Yellow and Green market types, black epidermis market types were genetically unique. Given the uniqueness and high genetic diversity resident in Villaconejos landraces, this germplasm pool should be considered as a genetic source for broadening the comparatively narrow genetic base of Group Cantalupensis and Inodorus melon market types, especially standard commercial Spanish Group Inodorus market types (e.g., Piel de Sapo, Rochet, and Canari).  相似文献   

Global Pollution: How Much Is Too Much?     

Jack T. Trevors  Milton H. Saier Jr 《Water, air, and soil pollution》2009,198(1-4):1-3

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Organic matter composition and the protist and nematode communities around anecic earthworm burrows     

Walter S. Andriuzzi  Phuong-Thi Ngo  Stefan Geisen  Aidan M. Keith  Kenneth Dumack  Thomas Bolger  Michael Bonkowski  Lijbert Brussaard  Jack H. Faber  Abad Chabbi  Cornelia Rumpel  Olaf Schmidt 《Biology and Fertility of Soils》2016,52(1):91-100

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Backcross introgression of plastomic factors controlling chilling tolerance into elite cucumber (Cucumis sativus L.) germplasm: early generation recovery of recurrent parent phenotype     

Vanessa S. Gordon  Jack E. Staub 《Euphytica》2014,195(2):217-234

Environmental stresses such as chilling temperatures can decrease germination, emergence, flower and fruit development, marketable yield, and postharvest fruit storage longevity in cucumber (Cucumis sativus L.). While response to chilling injury in cucumber is controlled by simple plastidic (maternal) and nuclear (paternal) factors, no chilling tolerant U.S. processing varieties are commercially available. Furthermore, even though three single nucleotide polymorphic sites have been identified as plastid components associated with chilling tolerance in cucumber, it is not known how these factors interact with nuclear factors controlling economically important traits. Therefore, an experiment was designed to evaluate the rate of recovery of the chilling susceptible (cytoplasm) genotype during introgression backcrossing (IB), where it was used as a recurrent parent after the initial mating to a line possessing chilling tolerant cytoplasm (donor parent). Phenotypic yield and quality trait data were collected on processing type backcross progeny (BC_1–5 and BC₂S₃) derived from an initial ‘Chipper’ (tolerant) × line M 29 (susceptible) mating, and rate of progression to the recurrent parent was determined by simple sequence repeat marker and morphological trait analyses. Substantial degrees of the recurrent parent phenotype and nuclear genome were recovered by the BC₂ generation (P = 0.001), with nearly complete recovery of recurrent parental traits and its nuclear genome occurring by the BC₃. General combining ability (GCA) of derived BC₂S₃ lines was significant for yield, yield/plant, length (L), diameter (D), and L:D ratios. The BC₂S₃ line GCA and rate of progression towards the recurrent parent for economically important traits suggests that elite chilling tolerant cucumber germplasm can be developed rapidly through IB and marker genotyping.  相似文献   

Bringing Agroforestry Technology to Farmers in Leyte, the Philippines: A Comparison of Two Extension Assistance Regimes     

Jack Baynes  John Herbohn  Iean Russell 《Small-Scale Forestry》2009,8(4):381-398

The response of farmers to personalised forestry and agroforestry extension assistance has not been well documented in the Philippines, thus providing the impetus for this research in which the effectiveness of extended on-farm assistance was compared with more limited assistance. In four municipalities in Leyte Island, farmers responded positively to an extended program which helped them overcome problems in germinating and growing seedlings and establishing trees on their land. A limited assistance program which sought to make use of farmers’ familiarity with growing and raising plants was relatively unsuccessful, with less preparation of tree planting sites and a higher rate of abandonment. Allowing farmers freedom to select planting sites and aspects of technical advice which suited their personal circumstances, encouraged a high degree of cooperation between extension staff and farmers, but 38% of farmers neglected post-planting weed control and 35% of sites were inappropriate for growing trees. Destruction of sites by flooding and grazing also caused farmers to blame extension staff even though these risks had been discussed with them beforehand. This suggested that a more interventionist approach would be appropriate for site selection although it may reduce recruitment. The number of farmers recruited through local government staff was low, but attendance by neighbours at locally held demonstrations was high, suggesting an avenue for further recruitment. Overall, the program was successful in shifting the initiative for further planting from extension staff to those farmers who received extended extension assistance.  相似文献   

四川阿坝辐射松引种长期森林健康风险初步评估   总被引：2，自引：0，他引：2  

李荣伟  肖育贵  周建华  吴宗兴  阎红  黄泉  刘千里 《林业研究》2008,19(2):85-100

Pinus radiata was introduced to the summer rainfall environments of Sichuan Province, China in the 1990s as a part of an afforestation pmgram for soil and water conservation in the arid and semi-arid river valley area of Aba Prefecture. Within this region a total area of 26 000 ha have been identified through climate matching as suitable and a further 63 000 ha potentially suitable for environmental plantings of P. radiata. The plantations are being established in widely separated small patches on steep and degraded slopes along the dry river valley The newly introduced P. radiata are exposed to two kinds of forest health risks： they may be attacked by （a） indigenous pathogens and pests against which they may not possess any resistance or （b） by inadvertently introduced foreign pests or pathogens. This paper presents a survey of the potential damaging pests and a preliminary assessment of forest health risks facing the P. radiata plantations over a much longer timeframe than the initial phase of introduction and early plantation establishment. An empirical appmach was adopted to evaluate forest health risks by a combination of literature review, examination of historical records of pest and disease outbreaks in the surrounding conifemus forests, field surveys and inspections, specimen collection and identification, and most importantly, expert analysis of the likelihood of attack by specific pests and pathogens and the subsequent impact of such attacks. The assessment identified some specific forest health risks to the long-term success of P. radiata introduction in this area. These risks are closely associated with the indigenous pests and pathogens of the two native pine species, P. tabulaeformis and P. armondii since these pests and pathogens are considered more likely to establish on P. radiata over time. Exotic pests and pathogens are of a quarantine concern at present. Based on the results of assessment, recommendations are pmvided to improve forest vigour and to reduce the forest health risks pos  相似文献   

Genetic association of cotton yield with its component traits in derived primitive accessions crossed by elite upland cultivars using the conditional ADAA genetic model     

Jack C. McCarty  Jixiang Wu  Johnie N. Jenkins 《Euphytica》2008,161(3):337-352

Boll number, lint percentage, and boll weight are three component traits for lint yield of upland cotton, Gossypium hirsutum L. Selecting high yielding lines or hybrids depends on the ability to dissect the genetic relationship of lint yield with these component traits. In this study, 14 day-neutral lines with desirable fiber quality derived from primitive accessions were top crossed with five commercial cultivars. The F₂ populations and parents were grown in one location in 1998 and two locations in 1999 at Mississippi State, MS. The F₃ populations and parents were grown in two locations in 2000. Lint yield and three component traits were measured and analyzed by the ADAA genetic model with the mixed model based conditional approach. Results showed that boll number or boll number with lint percentage or boll weight contributed to the majority of the phenotypic variance and variance components for lint yield. Boll number was more important than the other two component traits in terms of various genetic effects. The results also showed that the combination of boll number and boll weight greatly increased the contribution to lint yield even though boll weight itself had no significant contribution to lint yield compared to boll number alone. The genetic contribution effects were also predicted due to single component traits or their combinations for parents and crosses. The results revealed that the balanced selection of boll weight and boll number should be considered to obtain high yielding hybrids or pure lines. Contribution of the USDA-ARS in cooperation with the Mississippi Agric. and Forestry Exp. Stn. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by USDA, ARS and does not imply its approval to the exclusion of other products or vendors that may also be suitable.  相似文献   

A novel ultrasonographic assisted technique for desmotomy of the palmar/plantar annular ligament in horses          下载免费PDF全文

Pablo Espinosa DVM  Jorge E. Nieto DVM  PhD  DACVS  Jack R. Snyder DVM  PhD  DACVS  Larry D. Galuppo DVM  DACVS  Scott A. Katzman DVM  DACVS 《Veterinary surgery : VS》2017,46(5):611-620

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Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary          下载免费PDF全文

B Berisha  D Schams  D Rodler  F Sinowatz  MW Pfaffl 《Reproduction in domestic animals》2017,52(1):130-139

The aim of this study was to characterize expression patterns of hypoxia‐inducible factor‐1alpha (HIF1A) and vasohibin family members (VASH1 and VASH2) during different stages of ovarian function in cow. Experiment 1: Antral follicle classification occurred by follicle size and estradiol‐17beta (E2) concentration in the follicular fluid into 5 groups (<0.5, 0.5–5, 5–40, 40–180 and >180 E2 ng/ml). Experiment 2: Corpora lutea (CL) were assigned to the following stages: days 1–2, 3–4, 5–7, 8–12, 13–16 and >18 (after regression) of oestrous cycle and of pregnancy (months 1–2, 3–4, 6–7, >8). Experiment 3: Cows on days 8–12 were injected with a prostaglandin F2alpha (PGF) analogue and CL were collected before and 0.5, 2, 4, 12, 24, 48 and 64 hr after PGF injection. Expression of mRNA was measured by qPCR, steroid hormone concentration by EIA and localization by immunohistochemistry. HIF1A mRNA expression in our study increases significantly in follicles during final maturation. The highest HIF1A mRNA expression was detected during the early luteal phase, followed by a significant decrease afterwards. In contrast, the mRNA of vasohibins in small follicle was high, followed by a continuous and significant downregulation in preovulatory follicles. The obtained results show a remarkable inverse expression and localization pattern of HIF1A and vasohibins during different stages of ovarian function in cow. These results lead to the assumption that the examined factors are involved in the local mechanisms regulating angiogenesis and that the interactions between proangiogenic (HIF1A) and antiangiogenic (vasohibins) factors impact all stages of bovine ovary function.  相似文献   

Psychological Attributes of Ultramarathoners     

Katherine Buck  Jack Spittler  Alex Reed  Morteza Khodaee 《The Forestry Chronicle》2018,29(1):66-71

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