Genetic relationships and diversity among Brazilian cultivars and landraces of common beans (Phaseolus vulgaris L.) revealed by AFLP markers     

Fábio Luís Maciel  Sergio Echeverrigaray  Lee Tseng Sheng Gerald  Felipe Gobbi Grazziotin 《Genetic Resources and Crop Evolution》2003,50(8):887-893

The genetic variation and relationships among 31 accessions of Phaseolus vulgaris L., and two representatives of Vigna unguiculata L., were evaluated by AFLP analysis. A total of 263 DNA fragments across all materials were scored using nine primer combinations, averaging 32 per primer. More than 95% of the amplification products showed polymorphism, indicating high variation at the DNA level among these accessions. Pair-wise genetic similarity (Jaccard's coefficient) ranged from 0.553 to 0.840, with a mean of 0.765. Twenty-three accessions (70%) clustered into three groups. A majority of the commercial cultivars (91%) clustered within a single group, whereas the landraces were distributed along all the variation. An apparent correlation with phaseolin types was detected. Results of this study suggest that Brazilian landraces truly represent the overall genetic variability of Phaseolus vulgaris, confirming the multiple origins of these materials, and their potential as a source of variation for breeding programs.  相似文献   

Differential Rotation and Dynamics of the Solar Interior     

MJ Thompson  J Toomre  ER Anderson  HM Antia  G Berthomieu  D Burtonclay  SM Chitre  J Christensen-Dalsgaard  T Corbard  M DeRosa  CR Genovese  DO Gough  DA Haber  JW Harvey  F Hill  R Howe  SG Korzennik  AG Kosovichev  JW Leibacher  FP Pijpers  J Provost  EJ Rhodes  J Schou  T Sekii  PB Stark  PR Wilson 《Science (New York, N.Y.)》1996,272(5266):1300-1305

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.  相似文献   

Nutrient Concentrations and Leaf Chlorophyll of Yellow Passion Fruit Seedlings as a Function of Substrate Composition and Boron     

Raissa Rachel Salustriano da Silva Matos  Gabriel Barbosa da Silva Júnior  Adenaelson de Souza Marques  Maciel Lima Monteiro  Ítalo Herbert Lucena Cavalcante  Josy Anteveli Osajima 《Journal of plant nutrition》2015,38(13):1984-1994

This study evaluated nutrient accumulation of yellow passion fruit seedlings produced in substrates containing proportions of decomposed buriti (Mauritia vinifera Mart.) stem (DBS) and the boron in this process. Four experiments were conducted in Federal University of Piauí in Brazil. The experimental design was completely randomized in factorial scheme 6 × 2 + 1, referring to the substrates [DBS (100%), soil and sand 1:1 (20%) + DBS (80%), soil and sand 1:1 (40%) + DBS (60%), soil and sand 1:1 (60%) + DBS (40%), soil and sand 1:1 (80%) + DBS (20%), commercial substrate (additional treatment without boron) and soil, sand and manure 1:1:2] with (0.5 mg B.dm^?3) and without boron. The following variables were recorded: total nitrogen, total phosphorus, potassium, calcium and magnesium, boron, absorption efficiency index and chlorophyll. No influence concerning the substrate composition was noticed on nutritional status, but boron influenced on K and Mg of yellow passion fruit seedlings.  相似文献   

A translocation experiment for the conservation of maned sloths, Bradypus torquatus (Xenarthra, Bradypodidae)     

Adriano G. Chiarello  David J. Chivers  Maria Amélia F. Maciel  Mariel Bazzalo 《Biological conservation》2004,118(4):421-430

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Combined preputial advancement and phallopexy as a revision technique for treating paraphimosis in a dog     

SM Wasik  AM Wallace 《Australian veterinary journal》2014,92(11):433-436

A 7‐year‐old neutered male Jack Russell terrier‐cross was presented for signs of recurrent paraphimosis, despite previous surgical enlargement of the preputial ostium. Revision surgery was performed using a combination of preputial advancement and phallopexy, which resulted in complete and permanent coverage of the glans penis by the prepuce, and at 1 year postoperatively, no recurrence of paraphimosis had been observed. The combined techniques allow preservation of the normal penile anatomy, are relatively simple to perform and provide a cosmetic result. We recommend this combination for the treatment of paraphimosis in the dog, particularly when other techniques have failed.  相似文献   

Assessing resistance of ivermectin and moxidectin against nematodes in cattle naturally infected using three different methodologies     

Welber Daniel Zanetti Lopes  Weslen Fabricio Pires Teixeira  Gustavo Felippelli  Breno Cayeiro Cruz  Willian Giquelin Maciel  Vando Edésio Soares  Thais Rabelo dos Santos  Lucas Vinicius Shigaki de Matos  Flávia Carolina Fávero  Alvimar José da Costa 《Research in veterinary science》2014

The objective of this study was to evaluate the accuracy of the faecal egg count reduction test (FECRT) and the faecal egg count efficacy test (FECET) to assess the resistance status of ivermectin (630 μg/kg) and moxidectin (200 μg/kg), using the controlled efficacy test as a reference, and whether the results of the EPG are equivalent to the efficacy results from the parasitological necropsies. Two experiments were conducted. The results demonstrate that it was not possible to demonstrate that the EPG values were equivalent with the ivermectin and moxidectin efficacy obtained by parasitological necropsies, mainly if the phenomenon of parasites resistance is not advanced in a determined field population. Maybe the FECET technique would be possibly better than the FECRT. The high anthelmintic efficacy of 200 μg/kg moxidectin, in naturally infected cattle, against field population of nematodes that are resistant to 630 μg/kg ivermectin, was observed in this study.  相似文献   

Effect of Traditional and Liquid Smoke on the Quality of Sea Catfish Sausages (Sciades herzbergii,Bloch, 1794)     

Isabelly Barbosa de Araújo  Lito Jorge Raúl  Maria Inês Sucupira Maciel  Neide Kazue Sakugawa Shinohara 《Journal Of Aquatic Food Product Technology》2020,29(6):553-566

ABSTRACT

Smoking is a technique used to improve the sensory attributes and increase the shelf life of fish. The objective of the present study was to evaluate the nutritional, physicochemical, microbiological, and sensory characteristics of sausages made with sea catfish (Sciades herzbergii) fillets subjected to the traditional and liquid smoking processes. The traditionally smoked sausages showed lower (p < .05) moisture content and higher protein, fat, and mineral levels, higher weight loss during processing, higher pH, higher a* (red intensity), and lower L* (luminosity) values. The percentage of shrinkage, water holding capacity, water activity, hardness, cohesiveness, and elasticity were not influenced (p > .05) by the smoking methods tested. The microbial counts of both treatments were in compliance with the current Brazilian legislation. The sensory acceptance of the liquid smoked sausages was higher (p < .05) when compared to the traditionally smoked sausages for the attributes of color, aroma, texture, flavor, and overall acceptance, with scores ranging from 6 (liked slightly) to 7 (liked moderately). Between the two smoking methods tested, liquid smoking presented better sensory acceptance, easier execution, greater process control, and less environmental impact; thus, it is a viable alternative to the traditional smoking of sea catfish (Sciades herzbergii) sausages.  相似文献   

Quantity of IL‐2, IL‐4, IL‐10, INF‐γ, TNF‐α and KC‐Like Cytokines in Serum of Bitches With Pyometra in Different Stages of Oestrous Cycle and Pregnancy     

GS Maciel  RR Uscategui  VT de Almeida  MEF Oliveira  MAR Feliciano  WRR Vicente 《Reproduction in domestic animals》2014,49(4):701-704

The occurrence of the pyometra is most common in the first half of the dioestrus when there is decreased cellular immunity associated with increased serum concentration of progesterone in females. The aim of this study was to determine the immunological profile of bitches with pyometra, studying serum levels of IL‐2, IL‐4, IL‐10, IFN‐γ, KC‐like and TNF‐α and comparing them with those of healthy bitches in anoestrus, dioestrus and pregnant. Forty females were divided into four experimental groups: group 1 (G1): with pyometra (n = 10); group 2 (G2): bitches in the second week of gestation (n = 10); group 3 (G3): in anoestrus (n = 10); and group 4 (G4): in dioestrus (n = 10). The serum levels for IL‐2, KC‐like, INF‐γ and TNF‐α were similar for all experimental groups. The values obtained for IL‐10 were found increased (p < 0.001) in animals in dioestrus and pyometra compared with females in anoestrus and pregnant, and the levels of IL‐4 observed were significantly greater (p < 0.001) in bitches with pyometra when compared with others. The cytokine profile in animals with pyometra is similar to bitches in dioestrus for IL‐10 and had increase in IL‐4 for bitches with pyometra, which represents an anti‐inflammatory these cases. This suggests the presence of an immunosuppressive state in both cases, which may explain the propensity of bitches in dioestrus to be affected by pyometra and the severity of the disease on these animals.  相似文献   

Development of a Multiplex PCR for the Identification of Major Pathogenic Bacteria of Post‐Partum Endometritis in Dairy Cows     

SM Aghamiri  M Haghkhah  MR Ahmadi  HR Gheisari 《Reproduction in domestic animals》2014,49(2):233-238

Post‐partum period has an important role in cows' breeding due to its effects on reproductive efficiency and subsequent pregnancy. Escherichia coli, Trueperella pyogenes (Arcanobacterium pyogenes), Fusobacterium necrophorum and Prevotella melaninogenicus are recognized as major pathogens associated with uterine endometrial lesions. The objective of this study was to identify these pathogens using the polymerase chain reaction (PCR) as a culture‐independent sensitive method. A total of 172 cows were examined 25–35 days post‐partum, and 128 cows were examined at 2 weeks later (39–49 days post‐partum). Uterine discharges were collected by covered plastic infusion pipettes. The prevalence of endometritis was greater in the first examination than the second (35.5% vs. 16%). E. coli was detected in eight of the samples, T. pyogenes was detected in 13 of the samples and F. necrophorum was detected in 11 of the samples. There was no positive sample of P. melaninogenicus. Uterine contamination by T. pyogenes and F. necrophorum in the first examination was higher than the second examination. T. pyogenes affected as a tendency the prevalence of clinical endometritis in first examination. Primiparous cows showed 4.02 times higher odds of clinical endometritis compared with second‐parity cows in first examination. A multiplex PCR protocol as a simple, less expensive, fast assay was introduced to identify E. coli, T. pyogenes and F. necrophorum.  相似文献   

Leaf morphology of melon mediates feeding and oviposition preference,and immature survival of Liriomyza sativae (Blanchard) (Diptera: Agromyzidae)     

de Oliveira  Josielma Monteiro  da Silva Dias-Pini  Nívia  da Silva Melo  José Wagner  Saraiva  Wenner Vinicius Araújo  Muniz  Celli Rodrigues  da Costa Lima  Tiago Cardoso  Oliveira  Valter Rodrigues  de Sousa Maciel  Gabriela Priscila 《Phytoparasitica》2021,49(4):479-489

Phytoparasitica - To assess the influence of melon genotypes on the feeding and oviposition preferences and survival of L. sativae immatures, a study was carried out by offering 21 melon genotypes...  相似文献