Preliminary characterization of Lactococcus garvieae bacteriophage isolated from wastewater as a potential agent for biological control of lactococcosis in aquaculture     

Seyed Mahdi Ghasemi  Majid Bouzari  Giti Emtiazi 《Aquaculture International》2014,22(4):1469-1480

Lactococcosis, a significant emerging disease of fish caused by Lactococcus garvieae, has become one of the devastating problems due to its serious economic damage in aquaculture. The aim of this study was to isolate and characterize a lytic phage infecting L. garvieae as a potential bioagent for the treatment of lactococcosis. In this regard, one strain of L. garvieae was isolated from diseased rainbow trout, and then, following biochemical and molecular identifications, its specific phage, WWP-1, which was able to destroy L. garvieae cells through the lytic cycle, was isolated from a municipal wastewater sample. Transmission electron microscope revealed that the isolated phage possesses an icosahedral head and a non-contractile short tail, resembled to members of the family Podoviridae. Moreover, phage WWP-1 represented optimal antibacterial activity at temperatures ranging from 15 to 30 °C, suggesting that it could be very effective at rainbow trout rearing temperature. Restriction profile analysis revealed that NdeI can digest WWP-1 genome while EcoRI, EcoRV, and BamHI were incapable of cutting its DNA. According to the in vivo experiment result, WWP-1 could decrease mortality rate of infected rainbow trout in aquaculture. The results suggest that this naturally occurring bacteriophage could be considered as a promising agent to control the disease caused by L. garvieae strains in rainbow trout rearing.  相似文献   

Application of the consolidated species concept to identify the causal agent of strawberry anthracnose in Iran and initial molecular dating of the <Emphasis Type="Italic">Colletotrichum acutatum</Emphasis> species complex     

Kaivan Karimi  Asadollah Babai Ahari  Mahdi Arzanlou  Jahanshir Amini  Ilaria Pertot  Omar Rota-Stabelli 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(2):375-387

Anthracnose caused by Colletotrichum spp. is one of main strawberry diseases worldwide. The disease recently arrived in Iran and become epidemic in the Kurdistan province. A combination of morphological, molecular and pathogen-host interaction approaches (referred to the consolidated species concept) were applied to isolates from symptomatic strawberry tissues collected in Iran. Multi-gene phylogenetic analysis based on ITS-rDNA, beta-tubulin (TUB2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) identified the species as the S5 clade of Colletotrichum nymphaeae. No significant intraspecies variation was observed in terms of morphology and pathogenicity. No significant genetic variation was observed among the isolates using inter simple sequence repeat analysis (ISSR) and primer pair combination of ERIC1/BOX and ERIC2/BOX fingerprinting markers. Based on the results of this study, it can be postulated that the C. nymphaeae population in Iran established itself from a single origin due to a founder effect. More generally, molecular dating based on relaxed clocks indicates co-radiation of C. nymphaeae S5 and strawberry plants and suggests high reciprocal specificity between the host and pathogen.  相似文献   

A Molecular Survey of Campylobacter jejuni and Campylobacter Coli Virulence and Diversity     

Mahdi Ghorbanalizadgan  Bita Bakhshi  Anoshirvan Kazemnejad Lili  Shahin Najar-Peerayeh  Bahram Nikmanesh 《Iranian Biomedical Journal》2014,18(3):158-164

Background: The aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis of Campylobacter spp. isolated from children with diarrhea in Iran. Methods: A total of 200 stool specimens were obtained from children under 5 years during July 2012 to July 2013. Detection of C. jejuni and C. coli was performed by standard biochemical and molecular methods. The presence of virulence-associated genes and genetic diversity of isolates was examined using PCR and ERIC-PCR analyses. Results: A total of 12 (6%) Campylobacter spp. were isolated from patients including 10 (4.5%) C. jejuni and 2 (1.5%) C.coli. The flaA, cadF and ciaB genes were present in 100% of isolates, while no plasmid of virB11 gene was present in their genome. The prevalence of invasion-associated marker was 100% among C. coli and was not detected in C. jejuni isolates. The distribution of both pldA and the genes associated with cytolethal distending toxin (CDT) was 58.3% in C. jejuni isolates. Seven distinct ERIC-PCR profiles were distinguished in three clusters using ERIC-PCR analysis. Genotyping analysis showed a relative correlation with geographic location of patients and virulence gene content of isolates. Conclusion: To our knowledge, this is the first molecular survey of Campylobacter spp. in Iran concerning genotyping and virulence gene content of both C. jejuni and C. coli. ERIC-PCR revealed appropriate discriminatory power for clustering C. jejuni isolates with identical virulence gene content. However, more studies are needed to clearly understand the pathogenesis properties of specific genotypes. Key Words: Campylobacter jejuni, Campylobacter coli, Ddiarrhea, Virulence factors  相似文献   

Effect of microwave–chemical pre-treatment on compression characteristics of biomass grinds     

Mahdi Kashaninejad  Lope G. Tabil 《Biosystems Engineering》2011,108(1):36-45

The effect of microwave and microwave–chemical pre-treatments on densification characteristics and physical quality of pellets made from wheat and barley straw grinds were investigated. The ground wheat and barley straw samples were immersed in water, sodium hydroxide or calcium hydroxide solution at different concentrations (1 and 2% w/v) and then exposed to microwave radiation at three power levels (295, 603 and 713 W). Chemical composition and bulk and particle densities of samples were determined after pre-treatments. Pre-treated grinds were compressed in a plunger–die assembly with a force of 4000 N and compression and relaxation test data were recorded. The specific energy required for compression and ejection of pellets produced from untreated and pre-treated wheat and barley straw grinds was calculated. The tensile strength of the pellets was also evaluated to investigate the hardness of the pellets. Chemical composition analysis showed that microwave and chemical pre-treatment was significantly able to disintegrate the lignocellulosic structure of wheat and barley straw grinds. Data analysis also indicated that the pellets made from microwave–chemical pre-treated biomass grinds had a significantly higher density and tensile strength than the untreated or samples pre-treated by microwave and distilled water.  相似文献   

Zinc sorption–desorption by sand,silt and clay fractions in calcareous soils of Iran     

Mahdi Najafi Ghiri  Mahrooz Rezaei  Abdolmajid Sameni 《Archives of Agronomy and Soil Science》2013,59(9):945-957

Zinc sorption–desorption by sand, silt and clay fractions of six representative calcareous soils of Iran were measured. Sand, silt and clay particles were fractionated after dispersion of soils with an ultrasonic probe. Zinc sorption analysis was performed by adding eight rates of Zn from 6 to 120 μmol g^?1. For the desorption experiment, samples retained after the measurement of Zn sorption were resuspended sequentially in 0.01 M NaNO₃ solution and shaken for 24 h. Results indicated that Zn sorption by soil fractions increased in the order clay > silt > sand, and correlated negatively with CaCO₃ content and positively with cation exchange capacity (CEC) and smectite content. Results indicated that for all fractions, the Langmuir equation described the sorption rates fairly well. In contrast to sorption, Zn desorption from soil fractions increased in the order sand > silt > clay, and correlated positively with CaCO₃ content, CEC and smectite content. Results showed that parabolic diffusion and two constant equations adequately described the reaction rates of Zn desorption. In general, for all soils studied, the coarser the particle size, the less Zn sorption and more Zn desorption, and this reflects much higher risk of Zn leaching into groundwater or plant uptake in contaminated soils.  相似文献   

Nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) reduces N2O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain     

Churong Liu  Hongrun Liu  Xueqing Liu  Yushi Zhang  Ligang Wang  Dahai Guan  Mahdi M. Al-Kaisi  Zhaohu Li  Mingcai Zhang 《European Journal of Soil Science》2021,72(3):1270-1291

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Effect of hydrothermaled bran on physicochemical, rheological and microstructural characteristics of Sangak bread   总被引：1，自引：0，他引：1  

Laleh Mosharraf  Mahdi Kadivar  Mohammad Shahedi 《Journal of Cereal Science》2009,49(3):398-404

Increased consumption of whole grain products has been associated with decreased risk of health problems such as cardiovascular disease, diabetes and obesity. Phytate however, has always been a matter of concern, since it chelates minerals such as iron. Sangak is very popular Iranian flat bread invented five hundred years ago, made from 95% extraction flour. The bread is fermented but usually fermentation is not able to remove all phytate from the dough. In this study flours were first debranned; resulting bran was subjected to a hydrothermal process. The hydrothermaled (HT) brans were then incorporated in the Sangak flour. In the next step, physicochemical, rheological and microstructural characteristics of Sangak flour and dough prepared from two Iranian wheat varieties, Tajan and Back Cross of Roshan were investigated. Results indicated a reduction in phytate up to 55% in the samples. The resulting dough containing HT bran showed a higher development time and valorimetric value and was more stable than doughs made with normal bran. Dough made with HT bran showed a kind of protein matrix in which proteins and starch granules are oriented in a more non-ordered structure.  相似文献   

Wide hybridization and introgression breeding in safflower: Effectiveness of different selection methods     

Soheila Espanani  Mohammad Mahdi Majidi  Ghodratollah Saeidi  Hossein Alaei  Vahid Rezaei 《Plant Breeding》2019,138(6):846-861

Possibility of using interspecific hybridization to increase genetic diversity of drought tolerance in safflower (Carthamus tinctorius L.) and effectiveness of different selection methods is poorly understood. The main objectives of this study were to evaluate the effectiveness of (a) interspecific hybridization to expand genetic diversity in safflower for agronomic traits and drought tolerance in advance generations and (b) different selection methods (direct vs. indirect) for improving seed yield. Interspecific hybridizations were performed between species of C. tinctorius × C. palaestinus (TP), C. palaestinus × C. oxyacantha (PO) and C. tinctorius × C. oxyacantha (TO). Based on the field screening of genotypes in F3 generation, four groups of selected genotypes (derived from combination of direct and indirect selection under both drought and normal environments along with selection based on drought tolerance index (STI)) were evaluated in F4 and F5 generations. Indirect selection for seed yield was significantly more efficient than direct selection. Selection in stress environment resulted to more efficiency than selection in normal environment. No significant difference was observed between direct selection and selection based on STI. The result showed that TP was the best population to combine high seed yield and drought tolerance in safflower.  相似文献   

Nitrate leaching in a silage maize field under different irrigation and nitrogen fertilizer rates   总被引：4，自引：0，他引：4  

Mahdi Gheysari  Seyed Majid Mirlatifi  Mehdi Homaee  Gerrit Hoogenboom 《Agricultural Water Management》2009,96(6):946-954

Quantification of the interactive effects of nitrogen (N) and water on nitrate (NO₃) loss provides an important insight for more effective N and water management. The goal of this study was to evaluate the effect of different irrigation and nitrogen fertilizer levels on nitrate-nitrogen (NO₃-N) leaching in a silage maize field. The experiment included four irrigation levels (0.7, 0.85, 1.0, and 1.13 of soil moisture depletion, SMD) and three N fertilization levels (0, 142, and 189 kg N ha⁻¹), with three replications. Ceramic suction cups were used to extract soil solution at 30 and 60 cm soil depths for all 36 experimental plots. Soil NO₃-N content of 0-30 and 30-60-cm layers were evaluated at planting and harvest maturity. Total N uptake (NU) by the crop was also determined. Maximum NO₃-N leaching out of the 60-cm soil layer was 8.43 kg N ha⁻¹, for the 142 kg N ha⁻¹ and over irrigation (1.13 SMD) treatment. The minimum and maximum seasonal average NO₃ concentration at the 60 cm depth was 46 and 138 mg l⁻¹, respectively. Based on our findings, it is possible to control NO₃ leaching out of the root zone during the growing season with a proper combination of irrigation and fertilizer management.  相似文献   

Silver nitrate and adenine sulphate induced high regeneration frequency in the recalcitrant plant Cosmos bipinnatus using cotyledon explants     

Mahdi Jaberi  Behzad Gharehyazi  Mahmood Khosrowchahli  Ali Sharafi  Nafiseh Aboofazeli 《The Journal of Horticultural Science and Biotechnology》2018,93(2):204-208

Plant regeneration ability was studied in the medicinal-ornamental plant, Cosmos bipinnatus ‘Sonata white’, which is a dicotyledonous recalcitrant plant to shoot induction. Cotyledons were used as sources of explants to investigate plant regeneration. High frequency of direct shoot induction was obtained when BA (5 mg/l) and AgNO₃ (5 mg/l) were used in combination with 20 mg/l adenine sulphate (73.8%) in Murashige and Skoog (MS) medium. The highest shoot number per explant (5.7) was induced on MS medium supplemented with 5 mg/l BA, 5 mg/l AgNO₃, and 40 mg/l adenine. Eight week-old shoots were transferred to root induction media containing MS and half-strength MS medium with different concentration of IBA. The highest rate of root induction (70.8%) was obtained on half-strength MS medium with 1.5 mg/l IBA within four weeks. The plantlets were transferred to pot and kept in the greenhouse condition. Seventy percent of the plantlets successfully acclimatised.

Abbreviations: BA, 6-benzylaminopurine; IBA, Indole-3-butyric acid; MS, Murashige and Skoog; PGRs, plant growth regulators.  相似文献