Effects of blend of canola oil and palm oil on nutrient intake and digestibility,growth performance,rumen fermentation and fatty acids in goats          下载免费PDF全文

Kazeem Dauda Adeyemi  Awis Qurni Sazili  Mahdi Ebrahimi  Anjas Asmara Samsudin  Abd Rasak Alimon  Roselina Karim  Saiful Anuar Karsani  Azad Behnan Sabow 《Animal Science Journal》2016,87(9):1137-1147

The study examined the effects of blend of 80% canola oil and 20% palm oil (BCPO) on nutrient intake and digestibility, growth performance, rumen fermentation and fatty acids (FA) in goats. Twenty‐four Boer bucks were randomly assigned to diets containing 0, 4 and 8% BCPO on a dry matter basis, fed for 100 days and slaughtered. Diet did not affect feed efficiency, growth performance, intake and digestibility of all nutrients except ether extract. Intakes and digestibilities of ether extract, unsaturated fatty acids (FA) and total FA were higher (P < 0.05) while digestibility of C18:0 was lower (P < 0.05) in oil‐fed goats than the control goats. Total volatile FA, acetate, butyrate, acetate/propionate ratio and methane decreased (P < 0.05) with increasing BCPO but propionate, NH₃‐N and rumen pH did not differ between diets. Ruminal concentration of C18:0, n‐3 FA and total FA increased (P < 0.05) while C12:0, C14:0, C15:0 and n‐6 FA decreased with increasing BCPO. Analysis of the FA composition of Triceps brachii muscle showed that concentrations of C16:0, C14:0 and C18:2n‐6 were lower (P < 0.05) while C18:1n‐9, C18:3n‐3 and C20:5n‐3 were higher in oil‐fed goats compared with control goats. Dietary BCPO altered muscle lipids without having detrimental effects on nutrient intake and digestibility and growth performance in goats.  相似文献   

Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli     

Maghsoudi N  Khalilpour A  Kamali M  Zeinoddini M 《Iranian Biomedical Journal》2007,11(3):147-152

BACKGROUND: Viral protein-1 (VP1) is a major capsid protein of Coxsakievirus B3 (CVB3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems VP1 can be considered as a vaccine candidate against CVB3 infection. In this study, cDNA of VP1 was prepared, cloned into pET expression vector and the recombinant protein (VP1) was over expressed in E. coli. METHODS: The viruses were grown in suspension cultures of Vero cells with an input virus multiplicity of 10-50 plaque-forming units/cell. After observing complete cytopathic effect, the total RNA (cells and virus) was prepared for RT-PCR and by using specific primers, VP1 cDNA was amplified and ligated into pET vectors (32 a and 28 a). The recombinant vector was transferred into competent E. coli (BL-21) and after selection of proper colony, which carried correct cDNA within the vector; cells were cultured and induced with isopropyl B-D-thiogalactopyranoside, in order to express protein (VP1). The cultures were tested for presence of VP1 by SDS-PAGE and Western-Blotting analysis. RESULTS: Molecular techniques such as PCR which showed exact defined size of the VP1 (819 bp), restriction digestion and finally immunoblot analysis of over expressed protein; all confirmed the correct cloning and expression of VP1 in this research. CONCLUSION: In this research, full length of VP1 as major capsid protein of CVB3 was over expressed in E. coli which, can be used for further studies, including neutralizing antibody production against CVB3.  相似文献   

Application of bio-desalinization for reclamation of salt-affected soil under composted cow manure and deficit irrigation with saline water     

Kalanaki  Mahdi  Ritzema  Henk  Bamshad  Roghayeh  Jones  Edward  Fazilatnia  Mojtaba 《Paddy and Water Environment》2020,18(2):469-479

Paddy and Water Environment - Application of common leaching and flushing methods is often impractical due to freshwater scarcity, groundwater pollution and a lack of suitable drainage systems. To...  相似文献   

Effect of vermicompost on some morphological,physiological and biochemical traits of bean (Phaseolus vulgaris L.) under salinity stress     

Abdollah Beykkhormizi  Parvaneh Abrishamchi  Ali Ganjeali  Mahdi Parsa 《Journal of plant nutrition》2016,39(6):883-893

Vermicompost can play an effective role in plant growth and development and also in reducing harmful effects of various environmental stresses on plants due to its porous structure, high water storage capacity, having hormone-like substances and plant growth regulators and also high levels of macro and micro nutrients. The aim of this study was to investigate the effects of vermicompost and salinity interactions on some morphological and physiological features and concentration of mineral elements of bean (Phaseolus vulgaris L. cv. Light Red Kidney) cultivar. A factorial experimental with five different volumetric ratios of vermicompost and sand, including to: 0:100; 10:90; 25:75; 50:50 and 75:25 and four levels of salinity [20, 40, 60 and 80 mmol l^?1 sodium chloride (NaCl)], and control was conducted base on Randomized Complete Block Design with three replications. Bean seeds were sowed in plastic pots, the seedlings being sampled 42 days old (flowering stage).The results showed that vermicompost had significant effect on all studied traits under stress and non-stress (p ≤ 0.05).In this experiment, the vermicompost significantly increased the photosynthetic rate and concentrations of potassium (K⁺) and calcium (Ca²)⁺in leaf and root tissues. In salinity levels of 20, 40 and 60 mmol l^?1NaCl, all subjected ratios of vermicompost and in 80 mmol l^?1NaCl the ratios of 10% and 75% vermicompost, significantly ameliorated negative effects of salinity. In both stress and non-stress conditions, using 10% volume of vermicompost is recommended to improve the growth of bean plants.  相似文献   

Soil and water conservation in ethiopia     

Mahdi Osman  Petra Sauerborn 《Journal of Soils and Sediments》2001,1(2):117-123

The article shows results of an international and interdisciplinary project with the title ‘Rainfall and its Erosivity in Ethiopia’. The article contains information about the traditional soil conservation practices, the reasons for intervention with traditional soil conservation technologies, the historical background of instutionalized soil conservation activities, the institutional frame work for soil conservation activities and their achievements, and the different departments and organisations for soil and water conservation as far as soil erosion in Ethiopia is concerned. As a conclusion and recommendation, the major constraints of the past activities and the current situation including future trends were discussed.  相似文献   

Potassium isotherm partitioning based on modified quantity‐intensity relation and potassium buffering characterization of soils of North India     

Shabir A. Bangroo  Nayar A. Kirmani  Mohammad A. Bhat  Javaid A. Wani  Asif M. Iqbal  Zahoor A. Dar  Syed Sheraz Mahdi  Ajaz A. Malik 《植物养料与土壤学杂志》2021,184(1):112-122

Background : Potassium (K) availability in soil and plant uptake is restrained by the dynamic interactions among the different pools of K. Aims : To understand these interactions, a study was undertaken to assess the quantity–intensity (Q/I) and buffering characteristics of rainfed maize (Zea mays L.) growing soils. Ten contrasting soils were evaluated for K partitioning changes in exchangeable K (ΔEK) and non‐exchangeable K (ΔNEK) pools in the soil‐solution phase and buffering characteristics using a modified version of Q/I approach. Results : The partitioned Q/I isotherms showed strong adsorption with the increase in K concentration ratio (CR^K) and the changes due to ΔEK were higher than changes due to ΔNEK. Total buffering capacity (PBC^K) significantly correlated (r = 0.92, p <0.01) with clay content with a major share contributed by buffering capacity owing to non‐exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{NEK}}^K$) rather than exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{EK}}^K$). The fixation capacity (β) factor, the magnitude of added K converted into a non‐exchangeable pool, ranged from 41 to 63%, whereas release (α) factor, the magnitude of added K converted to the exchangeable pool, ranged from 19 to 36%. Both threshold solution K (CK_r) and threshold exchangeable K (EK_r) values were found to be high in Satran clay loam (S2) and lower in Doon silty clay loam (S3) soils. The equilibrium exchangeable K (EK_o) was found close to minimum exchangeable K (E_min) in Doon silty clay loam (S3) and Babaweyl sandy clay loam (S1) soils and overall E_min constituted about 8.94 to 0.57% of the EK_o. Conclusion : It may be concluded that K Q/I isotherm partitioning provides a valuable insight to assess the dynamic relations. The ratio of α/β (K recharge index) could be used to evaluate the K enrichment capacity of soil to K additions while EK_r and E_min can be potentially useful in the elucidation of exchangeable K as K fertility index especially in soils with poor K fertilizer management.  相似文献   

Association Study of rs1333040 and rs1004638 Polymorphisms in the 9p21 Locus with Coronary Artery Disease in Southwest of Iran     

Khadijeh Golabgir Khademi  Ali Mohammad Foroughmand  Hamid Galehdari  Saied Yazdankhah  Mahdi Pourmahdi Borujeni  Zahra Shahbazi  Parvaneh Dinarvand 《Iranian Biomedical Journal》2016,20(2):122-127

Background:

Coronary artery disease (CAD) is a multifactorial and heterogenic disease. Recently, genome-wide association studies have reported that rs1333040 (C/T) and rs1004638 (A/T) single nucleotide polymorphisms (SNPs) in the 9p21 locus have very strong association with CAD. This study aimed to examine these associations in Southwest of Iran.

Methods:

Blood samples were collected from 200 CAD patients and 110 healthy individuals with no CAD. The association of two SNPs with CAD was evaluated by PCR and restriction fragment length polymorphism.

Results:

Chi-square test showed no association between rs1333040 SNP and CAD (X²: 4.66, df: 2, P=0.09). Also, there was no association between rs1004638 SNP and CAD (X²: 0.27, df: 2, P=0.88).

Conclusion:

No association was observed between rs1333040 and rs1004638 SNPs in the 9P21 region and CAD in Southwest of Iran. Key Words: Coronary artery disease, Single nucleotide polymorphisms, Genetic association study, Iran  相似文献   

Supercritical carbon dioxide extraction of essential oils from Perovskia atriplicifolia Benth     

Pourmortazavi SM  Sefidkon F  Hosseini SG 《Journal of agricultural and food chemistry》2003,51(18):5414-5419

The supercritical fluid extraction (SFE) of the aerial parts of Perovskia atriplicifolia Benth. was studied. The effect of different parameters such as pressure, temperature, modifier identity, and modifier volume on the SFE of the plant was investigated. The extracts were analyzed by GC and GC-MS and compared with the essential oil obtained from P. atriplicifolia Benth by steam distillation. The supercritical extracts and the steam-distilled products had very different compositions. The main constituents of the oil obtained by steam distillation were 1,8-cineole, limonene, camphor, beta-caryophyllene, alpha-pinene, camphene, and alpha-humulene. On the other hand, the major components of SFE extracts were 1,8-cineole, limonene, camphor, beta-caryophyllene, gamma-cadinene, alpha-pinene, and alpha-terpinyl acetate. The results showed that increasing the temperature from 35 to 65 degrees C (at a constant pressure of 100 atm) drastically reduced the number of extracted components. Also, the number of extracted constituents and the percent of main analytes increased when lower pressures were used. Using different modifiers (e.g., methanol, ethanol, dichloromethane, and hexane) for the extraction of the plant at low pressure (100 atm) and temperature (35 degrees C) showed that hexane was more selective than the other modifiers.  相似文献   

Improvement in estimation of soil water retention using fractal parameters and multiobjective group method of data handling     

Mohammad Reza Neyshaburi  Hossein Bayat  Kourosh Mohammadi  Nader Nariman-Zadeh  Mahdi Irannejad 《Archives of Agronomy and Soil Science》2013,59(2):257-273

Soil water retention characteristic is required for modeling of water and substance movement in unsaturated soils and need to be estimated using indirect methods. Point pedotransfer functions (PTFs) for prediction of soil water content at matric suctions of 1, 5, 25, 50, and 1500 kPa were developed and validated using a data-set of 148 soil samples from Hamedan and Guilan provinces, Iran, by multiobjective group method of data handling (mGMDH). In addition to textural and structural properties, fractal parameters of the power-law fractal models for both particles and aggregates distributions were also included as predictors. Their inclusion significantly improved the PTFs’ accuracy and reliability. The aggregate size distribution fractal parameters ranked next to the particle size distribution (PSD) in terms of prediction accuracy. The mGMDH-derived PTFs were significantly more reliable than those by artificial neural networks but their accuracies were practically the same. Similarity between the fractal behavior of particle and void size distributions may contribute to the improvement of the derived PTFs using PSD fractal parameters. It means that both distributions of the pore and particle size represent the fractal behavior and can be described by fractal models.  相似文献   

The effect of feeding rations with different ratios of concentrate to alfalfa hay on blood hematological and biochemical parameters of farmed ostriches (Struthio camelus)     

Hossein Ali Ghasemi  Mehdi Kazemi-Bonchenari  Amir Hossein Khaltabadi-Farahani  Mahdi Khodaei Motlagh 《Tropical animal health and production》2013,45(7):1635-1640

A two-month trial was conducted to investigate the effects of pelleting rations with 70:30, 65:35, and 60:40 concentrate to high-quality roughage (alfalfa hay) ratios on hematological and biochemical parameters of ostriches. A total of 18 seven-month-old male ostrich chicks, initially weighing an average of 60–70 kg, were distributed into three different outdoor paddocks at a stocking density of six birds per paddock. In the morning, blood collection was made from the wing vein after about 12 h of fasting. All rations resulted in no significant change in hematological parameters. However, with increasing alfalfa inclusion rate, the heterophil/lymphocyte ratio tended to be lower (P?=?0.089); whereas the values of hemoglobin, packed cell volume (PCV), and mean corpuscular hemoglobin (MCH) tended to be higher (P values 0.069, 0.072 and 0.094, respectively). In terms of plasma biochemical parameters, increasing the ratio of concentrate to alfalfa hay in ostrich diets resulted in significant (P?<?0.05) depression in the values of glucose (up to 20.2 %), total cholesterol (up to 19.2 %), creatine kinase (up to 27.9 %), and aspartate aminotransferase (up to 29.9 %). Additionally, the 65:35 or 60:40 rations caused a decrease in low-density lipoprotein cholesterol level (27.9 and 42 %, respectively; P?=?0.008) compared to the 70:40 ration. Without exception, no rations affected the values of leukocyte subsets, total protein, albumin, globulin, uric acid, alanine aminotransferase, gamma glutamyl transferase, triglycerides, high-density lipoprotein cholesterol, and very low density lipoprotein. Based on our results, the concentrate to alfalfa ratio of 60:40 could be suggested as optimum ratio for good health conditions of juvenile ostriches.  相似文献