Zinc and Boron Application at Different Phenological Stages Alleviates Tree Growth,Fruit Yield and Quality of Sweet Orange Cv. ‘Blood Red’     

Walli  Sammen  Hafiz  Ishfaq Ahmed  Khan  Rashid Iqbal  Bashir  Muhammad Ajmal  Uddin  Sareer  Khan  Shakir Ullah  Ajmal  Usman  Hussain  Sajjad  Khalid  Muhammad Fasih 《Gesunde Pflanzen》2022,74(2):385-396

The deficiency of micronutrients, particularly zinc (Zn) and boron (B) has affected vegetative growth, yield and fruit quality of sweet orange in many citrus growing areas of Pakistan. The present study was conducted in 2015 and 2016 to determine the impact of Zn and B supplementation on growth and quality of sweet orange cv. ‘Blood red’. Pre-harvest foliar application of variant Zn and B levels was done at different phenological stages of fruit growth and development, i.e., full bloom, fruit set and premature stage. Results have concluded that pre-harvest foliar application of Zn and B augmented phosphorous, Zn, B, potassium, iron, and manganese contents in leaves, particularly Zn and B content from deficient to optimal level. Moreover, the combined application of Zn and B has also significantly enhanced vegetative characters such as tree height and spread along with reproductive characters, i.e., incremented numbers of fruit, fruit weight, total soluble solids, titratable acidity and ascorbic acid contents. However, among stage comparison, outcomes depicted that foliar application of Zn and B at full bloom?+?fruit set?+?premature stage resulted in maximum production with high quality fruits.

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Comparing the effectiveness of R genes in a 2-year canola–wheat rotation against Leptosphaeria maculans,the causal agent of blackleg disease in Brassica species     

Rashid  M. Harunur  Liban  Sakaria H.  Zhang  Xuehua  Parks  Paula S.  Borhan  Hossein  Fernando  W. G. Dilantha 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(3):573-586

European Journal of Plant Pathology - Blackleg, which is caused by the ascomycete fungus Leptosphaeria maculans, is a major disease of canola in Canada and worldwide. In Canada, canola is usually...  相似文献   

‘On-farm’ seed priming with zinc sulphate solution—A cost-effective way to increase the maize yields of resource-poor farmers   总被引：2，自引：0，他引：2  

D. Harris  A. Rashid  G. Miraj  M. Arif  H. Shah 《Field Crops Research》2007

The effect of adding zinc sulphate (ZnSO₄) to maize (Zea mays L.) growing on calcareous, Zn deficient soils in the North West Frontier Province of Pakistan was assessed in vitro, on-station and in on-farm trials. The zinc sulphate was added either as a powder to the soil or by soaking seeds for 16 h in dilute solutions prior to sowing. For the first time in maize, we separated the benefits of priming simply with water from those provided by added zinc.  相似文献   

PCR-RFLP analysis of the whole chloroplast DNA from three cultivated species of cotton (Gossypium L.)     

Rashid Ismael Hag Ibrahim  Jun-Ichi Azuma  Masahiro Sakamoto 《Euphytica》2007,156(1-2):47-56

Variations of the chloroplast DNA (cpDNA) from three of the four cultivated species of cotton (Malvaceae); Gossypium barbadense L., Gossypium hirsutum L., Gossypium arboreum L. and its synonym Gossypium nanking Meyen., were analyzed. Using specific set of primers, the whole circular cpDNAs from the four test species were amplified. These were subsequently digested with the use of seven restriction enzymes. The amplified fragments of the whole cpDNAs of the diploid cultivated cotton G. arboreum and its synonym G. nanking did not show any differences. However, the allotetraploid cultivated cottons G. barbadense and G. hirsutum, showed some fragment length differences directly visible after amplification and two types of restriction fragment length polymorphism (RFLP), the first appeared as slightly lengthened bands and the other as gain or loss of a restriction site. The results also showed that the chloroplast genomes of the allotetraploid cultivated cottons are highly similar to the diploid cultivated cottons tested in terms of length and digestion patterns. The detected amplified length differences, RFLPs and the restriction sites can be considered as species specific markers for the allotetraploid cultivated cottons, which could be a useful tool for future studies of the cpDNA of the genus Gossypium L.  相似文献   

Cytotoxicity and antibacterial activity of extractives from Wedelia calendulacea     

Mottakin AK  Chowdhury R  Haider MS  Rahman KM  Hasan CM  Rashid MA 《Fitoterapia》2004,75(3-4):355-359

The cytotoxicity and antibacterial activity of petroleum ether, chloroform and methanol extracts of Wedelia calendulacea were assayed by brine shrimp lethality bioassay and standardized disk diffusion method against 19 bacterial strains. Three diterpenes isolated from the plant were also evaluated for in vitro antibacterial activities. The LC50 for the crude extracts against the brine shrimp nauplii were found to be 4.59 microg/ml, 7.99 microg/ml and 14.88 microg/ml, respectively, whereas the positive control, vincristine sulfate showed an LC50 of 0.58 microg/ml. Among the crude extracts and pure compounds tested, (-)-kaur-16-en-19-oic acid isolated from the chloroform extract showed the highest inhibitory activity against most of the bacterial strains with mean zone of inhibition of 10-21 mm at 200 microg/disc.  相似文献   

Antiulcer activity of Mikania cordata     

Paul RK  Jabbar A  Rashid MA 《Fitoterapia》2000,71(6):701-703

The alkaloidal fraction obtained from an ethanolic extract of the leaves of Mikania cordata exhibited significant in vivo antiulcer activity in diclofenac sodium-induced gastric erosions in Long Evans rats.  相似文献   

Antibacterial and cytotoxic compounds from the bark of Cananga odorata     

Rahman MM  Lopa SS  Sadik G  Harun-Or-Rashid  Islam R  Khondkar P  Alam AH  Rashid MA 《Fitoterapia》2005,76(7-8):758-761

O-Methylmoschatoline, liriodenine and 3,4-dihydroxybenzoic acid isolated from the barks of Cananga odorata showed antibacterial activities against a number of Gram (+) and Gram (-) bacteria. The compounds also showed antifungal and cytotoxic activities.  相似文献   

A vaccine prepared from a non-pathogenic H5N1 influenza virus strain from the influenza virus library conferred protective immunity to chickens against the challenge with antigenically drifted highly pathogenic avian influenza virus     

Samad RA  Nomura N  Tsuda Y  Manzoor R  Kajihara M  Tomabechi D  Sasaki T  Kokumai N  Ohgitani T  Okamatsu M  Takada A  Sakoda Y  Kida H 《The Japanese journal of veterinary research》2011,59(1):23-29

Inactivated influenza virus vaccine prepared from a non-pathogenic influenza virus strain A/duck/Hokkaido/Vac-1/2004 (H5N1) from the virus library conferred protective immunity to chickens against the challenge of antigenically drifted highly pathogenic avian influenza virus (HPAIV), A/whooper swan/Hokkaido/1/2008 (H5N1). The efficacy of the vaccine was comparable to that prepared from genetically modified HPAIV strain deltaRRRRK rg-A/ whooper swan/Mongolia/3/2005 (H5N1), which is more antigenically related to the challenge virus strain, in chickens.  相似文献   

Biochemical and biophysical changes induced by fungicide sodium diethyl dithiocarbamate (SDD), in phytocystatin purified from Phaseolus mungo (Urd): a commonly used Indian legume     

Sharma S  Rashid F  Bano B 《Journal of agricultural and food chemistry》2005,53(15):6027-6034

Phytocystatins are the plant thiol protease inhibitors involved in several reaction mechanisms of the plant system like regulation of proteolytic activity and storage of proteins. Biochemical and biophysical changes induced by fungicide SDD in phytocystatin purified from Phaseolus mungo have been investigated in terms of mass spectroscopy, Fourier transform infrared spectroscopy, and fluorescence spectroscopy, at pH 7.0, with varying fungicide concentrations (1-9 mM) and a time of incubation ranging from 2 to 8 h at 37 degrees C, with a fixed cystatin concentration (1.5 mM). Reactive oxygen species responsible for inhibitor damage were also investigated, and thiourea was found to scavenge the free radicals generated by SDD. FTIR analysis indicates a significant conformational transition from alpha-helix to beta-sheet structure; quenching of fluorescence is evident by fluorescence spectroscopy. The activity assay showed a decrease in inhibitory activity, as well as a fragmentation of the inhibitor was observed in electrophoresis. Results obtained implicate that exposure of phytocystatins to SDD involves physicochemical changes in cystatins leading to damage and a decrease in the activity of the inhibitor.  相似文献   

Nutrient Uptake,Water Relations,and Yield Performance lf Different Wheat Cultivars (Triticum aestivum L.) under Salinity Stress     

Rai Altaf Hussain  Rashid Ahmad  Fahim Nawaz 《Journal of plant nutrition》2015,38(13):2139-2149

A pot experiment was conducted in the wire house of Department of Crop Physiology, University of Agriculture, Faisalabad to evaluate the effect of salinity stress on water relations, nutrient uptake and yield of six local spring wheat cultivars. The seeds were sown in plastic pots (25 × 15 cm) and experiment was laid out in a randomized complete block design in factorial arrangement with three repeats. De-ionized water was used as control treatment while salinity stress was imposed by irrigating plants with sodium chloride (NaCl) solution of 10 mM at tillering, stem elongation, anthesis, and grain development stages. Results of the study demonstrated that salinity stress decreased water potential by 32%, osmotic potential by 12%, and relative water contents by 20% as compared to control treatment. The nitrogen (N) uptake was decreased by 36% under salinity stress, while phosphorous (P) and potassium (K) uptake were decreased by 56% and 42%, respectively. The yield of wheat plants was also significantly reduced under salinity stress. It reduced grain yield by 25% and grain weight by 7%. The response of different cultivars was also different to salinity stress as cultivars ‘Lasani-08’ and ‘FSD-08’ were found to be more tolerant as compared to other cultivars.  相似文献