Development of a pen-site test kit for the rapid diagnosis of H7 highly pathogenic avian influenza   总被引：1，自引：0，他引：1  

Manzoor R  Sakoda Y  Sakabe S  Mochizuki T  Namba Y  Tsuda Y  Kida H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):557-562

As well as H5 highly pathogenic avian influenza viruses (HPAIV), H7 HPAIV strains have caused serious damages in poultry industries worldwide. Cases of bird-to-human transmission of H7 HPAIV have also been reported [11]. On the outbreak of avian influenza, rapid diagnosis is critical not only for the control of HPAI but also for human health. In the present study, a rapid diagnosis kit based on immunochromatography for the detection of H7 hemagglutinin (HA) antigen of influenza A virus was developed using 2 monoclonal antibodies that recognize different epitopes on the H7 HAs. The kit detected each of the tested 15 H7 influenza virus strains and did not react with influenza A viruses of the other subtypes than H7 or other avian viral and bacterial pathogens. The kit detected H7 HA antigen in the swabs and tissue homogenates of the chickens experimentally infected with HPAIV strain A/chicken/Netherlands/2586/03 (H7N7). The results indicate that the present kit is specific and sensitive enough for the diagnosis of HPAI caused by H7 viruses, thus, recommended for the field application as a pen-site test kit.  相似文献   

Comparative efficacy of diminazene diaceturate and diminazene aceturate for the treatment of babesiosis in horses     

Rashid HB  Chaudhry M  Rashid H  Pervez K  Khan MA  Mahmood AK 《Tropical animal health and production》2008,40(6):463-467

The present work was conducted to study the comparative efficacy of two anti-protozoan (babesicidal) drugs on the recovery and health of horses. A total of 80 horses, showing typical clinical symptoms of the disease, were selected for this study; the presence of babesiosis was confirmed through blood smear examination. These animals were divided into two groups i.e. A and B. Horses of Group A, were treated with diminazene diaceturate, while horses of Group B were treated with diminazene aceturate. Efficacy of the drugs was determined by the reversal of clinical signs and a negative blood smear examination. The efficacy of diminazene diaceturate was demonstrated to be 80% while diminazene aceturate was found to have 90% efficacy against babesiosis.  相似文献   

Antibodies to prion and Acinetobacter peptide sequences in bovine spongiform encephalopathy     

Wilson C  Hughes L  Rashid T  Cunningham P  Bansal S  Ebringer A  Ettelaie C 《Veterinary immunology and immunopathology》2004,98(1-2):1-7

An amino acid sequence homology has been identified between the bovine prion sequence (RPVDQ) and the Acinetobacter calcoaceticus enzyme, uridine-diphosphate-N-acetyl glucosamine-1-carboxy-vinyl-transferase which also contains (RPVDQ). Class-specific IgA, IgG and IgM antibodies against synthetic peptides containing the structurally related sequences present in bovine prion and A. calcoaceticus were measured in 189 bovine spongiform encephalopathy (BSE) positive cattle, 127 BSE negative cattle and 87 healthy control animals using an ELISA technique. Class-specific IgA, IgG and IgM antibodies against the structurally related synthetic peptides were significantly elevated in BSE positive cattle when compared to BSE negative cattle (P < 0.001) and healthy control animals (P < 0.001). These autoantibodies may have a role in the pathogenesis of BSE.  相似文献   

Experimental study and numerical simulation of denitrification in saturated porous media     

Rashid I. Allayla  Niaz Mohammed  Tahir Husain 《Water, air, and soil pollution》1992,61(1-2):75-93

The effect of denitrification on the behavior of nitrate in saturated sandy soil was studied in the laboratory and the results were compared with the temporal and spatial concentration of nitrate in nonreactive (without denitrification) and reactive (with denitrification) cases. A laboratory model was fabricated to study steady one-dimensional flow and to transport nitrate with or without denitrification. Denitrification at various rates has been simulated with varied C∶N ratios and detention time. Retardation constant, dispersivity, and degradation constants have been computed using existing analytical models supporting adsorption and zero and/or first order production or decay. It was found that such analytical models can be used to fit the concentration of nitrate in saturated porous media for a C∶N ratio between 2 and 5.  相似文献   

Mutagenicity assays with (2,4-dichlorophenoxy)acetic acid--amino acid conjugates     

K A Rashid  R O Mumma 《Journal of agricultural and food chemistry》1983,31(6):1371-1372

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Intergenerational cost–benefit analysis and marine ecosystem restoration     

Ussif Rashid Sumaila 《Fish and Fisheries》2004,5(4):329-343

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Phosphorus starvation induced root-mediated pH changes in solublization and acquisition of sparingly soluble P sources and organic acids exudation by Brassica cultivars   总被引：1，自引：0，他引：1  

Akhtar M. SHAHBAZ  Yoko OKI  Tadashi ADACHI  Yoshiyuki MURATA  Md Harunar Rashid KHAN 《Soil Science and Plant Nutrition》2006,52(5):623-633

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Zinc requirement of corn grown on two calcareous soils of Pakistan     

A. Rashid  E. Rafique 《植物养料与土壤学杂志》1989,152(5):405-408

In greenhouse studies, corn (Zea mays L.) growth increased with Zn fertilization of two alkaline calcareous soils. Zinc concentration and total uptake increased with Zn application. Very high correlations were recorded between plant tissue Zn concentration, total Zn uptake and soil Zn levels determined by DTPA and AB-DTPA soil tests. Correlation between Zn concentration in plants and relative yield was poor. However, close relationships were revealed between extractable soil Zn and relative yield. Near maximum dry matter yield of corn was associated with a fertilizer rate of 2 mg Zn/kg soil. Plant tissue Zn-requirement was 27 mg/kg in 15 days old plants and 32 mg/kg in corn shoots of 40 day age. Critical soil test Zn level was 1.2 mg/kg by DTPA and 1.7 mg/kg by AB-DTPA method. Use of AB-DTPA soil test is suggested for evaluating Zn status of calcareous soils.  相似文献   

An H5N1 highly pathogenic avian influenza virus that invaded Japan through waterfowl migration     

Kajihara M  Matsuno K  Simulundu E  Muramatsu M  Noyori O  Manzoor R  Nakayama E  Igarashi M  Tomabechi D  Yoshida R  Okamatsu M  Sakoda Y  Ito K  Kida H  Takada A 《The Japanese journal of veterinary research》2011,59(2-3):89-100

In 2010, an H5N1 highly pathogenic avian influenza virus (HPAIV) was isolated from feces of apparently healthy ducks migrating southward in Hokkaido, the northernmost prefecture of Japan. The H5N1 HPAIVs were subsequently detected in domestic and wild birds at multiple sites corresponding to the flyway of the waterfowl having stopovers in the Japanese archipelago. The Hokkaido isolate was genetically nearly identical to H5N1 HPAIVs isolated from swans in the spring of 2009 and 2010 in Mongolia, but less pathogenic in experimentally infected ducks than the 2009 Mongolian isolate. These findings suggest that H5N1 HPAIVs with relatively mild pathogenicity might be selected and harbored in the waterfowl population during the 2009-2010 migration seasons. Our data provide "early warning" signals for preparedness against the unprecedented situation in which the waterfowl reservoirs serve as perpetual sources and disseminators of HPAIVs.  相似文献   

Recent Insights into Signaling Responses to Cope Drought Stress in Rice   总被引：1，自引：0，他引：1  

Muhammad Mahran Aslam  Muhammad Abdul Rehman Rashid  Mohammad Aquil Siddiqui  Muhammad Tahir Khan  Fozia Farhat  Shafquat Yasmeen  Imtiaz Ahmad Khan  Shameem Raja  Fatima Rasool  Mahboob Ali Sial  Zhao Yan 《水稻科学》2022,29(2):105-117

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