Immunologic studies on three ram epididymitis bacterins   总被引：3，自引：0，他引：3  

B L Swift  L R Maki 《The Cornell veterinarian》1968,48(4):659-665

  相似文献   

Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum     

Masahito WATANABE  Mirina KOBAYASHI  Masaki NAGAYA  Hitomi MATSUNARI  Kazuaki NAKANO  Miki MAEHARA  Gota HAYASHIDA  Shuko TAKAYANAGI  Rieko SAKAI  Kazuhiro UMEYAMA  Nobuyuki WATANABE  Masafumi ONODERA  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2015,61(3):169-177

Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum.  相似文献   

A neonatal calf with concurrent meningoencephalitis by Enterobacter cloacae and enteritis by attaching and effacing Escherichia coli (O128)     

Seimiya YM  Murakami M  Takahashi M  Sasaki K  Miyazaki H  Kawashima K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):445-448

A neonatal calf developed nervous symptoms followed by diarrhea. The principal pathological changes were fibrinopurulent meningitis with necrosis of the cerebral parenchyma, and attaching and effacing lesions in the intestine. Cerebral necrosis was frequently associated with vascular changes. Enterobacter cloacae was isolated from the brain and Escherichia coli (O128) from the intestinal content. These suggest that cerebral and intestinal lesions were caused by the isolated organisms, and that most necrosis of the cerebral parenchyma might be infarctive.  相似文献   

The infectivity and pathogenicity of a group 2 bovine coronavirus in pups     

Kaneshima T  Hohdatsu T  Hagino R  Hosoya S  Nojiri Y  Murata M  Takano T  Tanabe M  Tsunemitsu H  Koyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):301-303

Canine respiratory coronavirus (CRCoV), which is more closely related to the bovine coronavirus (BCoV), has recently been detected in dogs. In this study, we examined whether BCoV was capable of infecting and exhibiting pathogenicity in dogs. Three 1-month-old pups were oronasally given field isolates of BCoV, and were kept together with 2 control animals. As a result, increases in BCoV-neutralizing antibody titers were confirmed in all pups in the challenged and control groups. Moreover, the virus gene was also detected in oral and rectal swabs by RT-PCR. These results indicate that BCoV infects dogs, and easily infects other dogs that are kept together. However, no clinical symptoms such as respiratory symptoms and diarrhea were observed.  相似文献   

Double‐stranded RNA‐mediated silencing of the white spot syndrome virus VP28 gene in kuruma shrimp,Marsupenaeus japonicus     

Raja Sudhakaran  Tomoya Kono  Mari Inada  Shogo Okugawa  Maki Yoshimine  Terutoyo Yoshida  Masahiro Sakai  Toshiaki Itami 《Aquaculture Research》2011,42(8):1153-1162

  相似文献   

Interleukin-12, interferon-gamma and interleukin-4 gene expression in cats infected with Toxoplasma gondii.     

T Koyama  Y Omata  Y Maki  Y Toyoda  A Saito 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(7):819-821

  相似文献   

Bacterial degradation of antibiotic residues in marine fish farm sediments of Uranouchi Bay and phylogenetic analysis of antibiotic-degrading bacteria using 16S rDNA sequences   总被引：3，自引：1，他引：3  

Teruya Maki  Hiroshi Hasegawa  Hiroyuki Kitami  Kyoko Fumoto  Yukihiro Munekage  Kazumasa Ueda 《Fisheries Science》2006,72(4):811-820

ABSTRACT:   Antibiotic residues in marine sediments of fish farms negatively influence microbial ecologic systems. The microbial degradation of antibiotic residues was experimentally examined in the marine sediments of Uranouchi Bay, to which one of five antibiotics was added. After incubation reducing physical factors, ampicillin, doxycycline, oxytetracycline, and thiamphenicol were significantly degraded, while josamycin maintained most of the initial amounts. The isolates resistant to ampicillin, josamycin, oxytetracycline, or thiamphenicol degraded each antibiotic in wide ranges of degrees, whereas the isolates degrading doxycycline were not obtained. Microbial degradation may contribute to the disappearance of ampicillin, doxycycline, oxytetracycline, and thiamphenicol in the fish farm. In contrast, the disappearance of josamycin would depend on physical factors, but the bacteria degrading josamycin at least exist in the marine sediments. Phylogenetic analysis using 16S rDNA sequences demonstrated that the antibiotic-resistant isolates formed several clusters in the Gram-positive bacterial group, the Flavobacterium–Cytophaga–Bacteroides group, and the proteobacteria subdivisions. The antibiotic-resistant bacterial population would be composed of various species including ubiquitous coastal bacterial groups. Several species of antibiotic resistant bacteria show antibiotic degradation activities, and appear to contribute to the disappearance of antibiotics in Uranouchi Bay.  相似文献   

Efficacy of soluble recombinant FliC protein from Salmonella enterica serovar enteritidis as a potential vaccine candidate against homologous challenge in chickens     

Okamura M  Matsumoto W  Seike F  Tanaka Y  Teratani C  Tozuka M  Kashimoto T  Takehara K  Nakamura M  Yoshikawa Y 《Avian diseases》2012,56(2):354-358

FliC, the flagellin antigen of Salmonella Enteritidis, was tested as a vaccine candidate for protective effect against a homologous challenge in chickens. After immunization with recombinant FliC (rFliC) or administration of phosphate-buffered saline (PBS) at 56 days old, the chickens were challenged with 10(9) colony-forming units of Salmonella Enteritidis at 76 days old. The vaccinated birds showed significantly decreased bacterial counts in the liver and cecal contents compared to those administered PBS at 7 days postchallenge, but the protection was partial. The replication experiment also showed a similar result. In both experiments, vaccination induced an increased level of serum anti-rFliC IgG, which was also reactive to the native flagella. The intestinal IgA level was slightly higher in the vaccinated birds than in the control. However, neither the proliferative response nor interferon-gamma secretion of splenic cells upon stimulation with rFliC was induced. Therefore, the effect of rFliC as a vaccine is limited, and further improvement is needed.  相似文献   

The effect of M‐phase stage‐dependent kinase inhibitors on inositol 1,4,5‐trisphosphate receptor 1 (IP3R1) expression and localization in pig oocytes          下载免费PDF全文

Anucha Sathanawongs  Katsuyoshi Fujiwara  Tsubasa Kato  Masahiko Hirose  Maki Kamoshita  Richard J. H. Wojcikiewicz  Jan B. Parys  Junya Ito  Naomi Kashiwazaki 《Animal Science Journal》2015,86(2):138-147

At fertilization, inositol 1,4,5‐trisphosphate receptor type 1 (IP₃R1) has a crucial role in Ca²⁺ release in mammals. Expression levels, localization and phosphorylation of IP₃R1 are important for its function, but it still remains unclear which molecule(s) regulates IP₃R1 behavior in pig oocytes. We examined whether there was a difference in localization of IP₃R1 after in vitro or in vivo maturation of pig oocytes. In mouse oocytes, large clusters of IP₃R1 were formed in the cortex of the oocyte except in a ring‐shaped band of cortex adjacent to the spindle. However, no such clusters of IP₃R1 were observed in pig oocytes and there was no difference in its localization between in vitro and in vivo matured oocytes. We next tried to clarify which factor(s) regulates IP₃R1 localization, phosphorylation and expression using M‐phase stage‐dependent kinase inhibitors. Our results show that treatments with roscovitine (p34^cdc2 kinase inhibitor) or U0126 (mitogen‐activated protein kinase inhibitor) did not affect IP₃R1 expression or localization in pig oocytes, although the latter strongly inhibited phosphorylation. However, treatment with BI‐2536, an inhibitor of polo‐like kinase 1 (Plk1), dramatically decreased the expression level of IP₃R1 in pig oocytes in a dose‐dependent manner. From these results, it is suggested that Plk1 is involved in the regulation of IP₃R1 expression in pig oocytes.  相似文献   

Analysis of fecal nutrients and particle size in captive proboscis monkeys (Nasalis larvatus) fed seasonal dietary foliage at a Japanese zoo     

Ryuta Kawasaki  Maki Kawakita  Kenta Kurosawa  Sanae Asano  Hiroshi Kajikawa 《Animal Science Journal》2021,92(1):e13612

One male and three female captive proboscis monkeys (Nasalis larvatus) were used as test animals to compare fecal nutrients after being fed seasonal foliage. Foliage and fecal samples were collected during three seasons (spring, summer, and winter). We analyzed crude ash (CA), ether extract (EE), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL). In addition to the above components, for the fecal samples, we examined total fecal nitrogen (TFN), nitrogen in fecal NDF (NDF-N), metabolic fecal nitrogen (MFN), and particle size distribution (MPS). Seasonal differences in foliage components were observed, with NDF and ADF being lowest in spring (p < 0.05). Fecal NDF and ADF also tended to be lowest in spring. Further, the distribution of fecal particle size indicated that MPS tended to increase in winter. The mean fecal NDF content (47.4%) for all three seasons measured in this study was as much as 1.3 times lower than previously reported values in free-ranging specimens. These data provide useful information that can be used to find some suitable food items for the endangered highly folivorous monkeys in captivity.  相似文献