Effect of soluble glass bolus administration on the blood selenium concentration in grazing Philippine goats under backyard conditions     

Maki HAYASHIDA  Edgar A. ORDEN  Emilio M. CRUZ  Libertado C. CRUZ  Tsutomu FUJIHARA 《Animal Science Journal》2006,77(6):595-602

The present study was conducted to determine the effects of selenium (Se) soluble glass bolus (SGB) on blood Se concentrations of mixed‐bred (native × Anglo Nubian) pregnant does weighing between 15 and 52 kg and grazing on natural pastures in the Philippines. The control group, consisting of 28 does, was not administered with SGB, while 23 does were administered with SGB every 6 months for a period of 12 months. Forage and blood samples in both groups were collected every 2 months. Their Se, sulfur, calcium, phosphorus, magnesium, copper and zinc concentrations were analyzed using the wet digestion method. The results showed that the grazed forage had concentrations of all these minerals that were far higher than the accepted critical lower levels. The Se concentration was higher (P < 0.05) in the dry season than in the rainy season, especially in the whole blood collected from the does and newborn kids. In both groups the Se concentration in the kids tended to increase during the suckling period but decreased after weaning. Kids born to the SGB does had higher whole blood Se concentrations (P < 0.05) than those born to the control does. However, the administration of SGB to the does did not affect any blood mineral concentrations except for Se in the grazing does and their kids. The animals did not exhibit any clinical signs of mineral deficiencies or toxicities during the experimental period.  相似文献   

Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage     

Masanori TOHNO  Hisami KOBAYASHI  Masaru NOMURA  Maki KITAHARA  Moriya OHKUMA  Ryuichi UEGAKI  Yimin CAI 《Animal Science Journal》2012,83(2):111-120

Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches.  相似文献   

Persistence of antibiotics in bovine mammary secretions following intramammary infusion at cessation of milking     

S. P. Oliver  T. M. Lewis  M. J. Lewis  H. H. Dowlen  J. L. Maki 《Preventive veterinary medicine》1990,9(4):301-311

A study was conducted to determine the persistence of antibiotic preparations for use in nonlactating cows in bovine mammary secretions following intramammary infusion at cessation of milking. Five commercially available antibiotic formulations were evaluated using 311 cows. All quarters of each cow were sampled once only during the nonlactating period and most cows were sampled at or near parturition. Antibiotic residues were detected qualitatively by the Bacillus stearothermophilus disc assay. Great variation between different antibiotics in persistence in mammary secretion was observed. In general, mammary secretions from most mammary glands infused with cloxacillin or penicillin-dihydrostreptomycin were positive at 28–35 days after infusion and some were positive at 42–49 days after infusion. On the other hand, <13% of mammary secretions at 7 days after infusion of novobiocin and 50% of mammary secretions at 14 days after infusion of penicillin-novobiocin were positive for antibiotics. Cephapirin benzathine persisted for about 21 days after infusion. Some samples that were positive for antibiotics after initial testing were negative following heating of samples, suggesting that component(s) of dry secretion can inhibit growth of B. stearothermophilus and influence the interpretation of results. Colostrum samples from all quarters except one were negative for antibiotics. These data suggest that nonlactating-cow antibiotic formulations persist primarily during the early to mid-nonlactating period. Based upon present methods of formulation, it would appear that antibiotic preparations for use in nonlactating cows most likely provide little protection during the periparturient period, at a time when mammary glands are highly susceptible to new intramammary infections.  相似文献   

Plus tree of Cryptomeria japonica D. Don with a heterozygous male-sterility gene     

Maki Saito  Hideaki Taira 《Journal of Forest Research》2005,10(5):391-394

To find plus tree clones of Cryptomeria japonica that are heterozygous for a male-sterility gene (Aa), we crossed a homozygous male-sterile tree (aa) with 63 clones. Male sterility in this case is controlled by a recessive allele at a single gene locus and is expressed only in homozygotes. All F₁ seedlings obtained by crossing the male-sterile mother tree and 62 out of the 63 clones produced pollen. In contrast, F₁ seedlings obtained from the crossing between the male-sterile mother tree and a plus tree clone, Ohara 13, produced 64 male-sterile individuals and 52 fertile individuals. The segregation ratio fitted the expected 1 : 1 ratio according to a chi-square test. These results clearly demonstrate that the Ohara 13 clone is heterozygous for a male-sterility gene.  相似文献   

Hardness distribution on wood surface     

Seiji Hirata  Masamitsu Ohta  Yasuo Honma 《Journal of Wood Science》2001,47(1):1-7

For better understanding wood hardness, we developed a new hand-operated hardness tester that works with Brinell's method. With this tester we investigated the hardness distribution of wood minutely using a 2mm diameter ball tip. The following results were obtained: (1) On preliminary examination with medium-density fiberboard, we found that the value of Brinell's hardness decreased with the increase in the tip ball's diameter but that it was almost constant with the indenting velocity. (2) By using a small diameter tip, the difference of the hardness became clearer between earlywood and latewood. (3) With radial and tangential sections, we obtained hardness distribution patterns similar to those of wood grain. It seems that the hardness distribution reflects the distribution of density on wood surfaces.  相似文献   

Estimating aboveground carbon using airborne LiDAR in Cambodian tropical seasonal forests for REDD+ implementation     

Tetsuji Ota  Tsuyoshi Kajisa  Nobuya Mizoue  Shigejiro Yoshida  Gen Takao  Yasumasa Hirata  Naoyuki Furuya  Takio Sano  Raul Ponce-Hernandez  Oumer S. Ahmed  Heng Sokh  Vuthy Ma  Eriko Ito  Jumpei Toriyama  Yukako Monda  Hideki Saito  Yoshiyuki Kiyono  Sophal Chann  Nang Ket 《Journal of Forest Research》2015,20(6):484-492

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Increased ectopic fat cells in the longitudinal muscularis layer of the oviduct isthmus in obese Japanese Black cows     

Sachiko Maki  Hiroya Kadokawa 《Animal Science Journal》2014,85(3):207-212

In obese humans, mesenchymal stem cells differentiate to become ectopic fat cells in muscles. These ectopic fat cells inhibit the contraction of vascular smooth muscles. Stem cells have been recently identified in the human oviduct, a structure important in reproduction. We therefore investigated the number of Oil Red O (ORO)‐positive cells in the oviducts of control Japanese Black cows (n = 6; body condition score [BCS], 3.0 on a 5‐point scale) compared to those with diet‐induced obesity (n = 5; BCS, 4.0). We stained the ampulla and isthmus collected on the second day after ovulation with ORO and then counted the positive cells in each layer in 10 cross‐sections of the ampulla or isthmus. The obese group (23.4 ± 3.4 in the 10 sections) had larger numbers of ORO‐positive cells in the longitudinal muscularis of the isthmus (P < 0.05) than did the control group (15.0 ± 2.4). ORO‐positive cells were also observed in all other layers of the isthmus and ampulla; however, the number of cells in these layers did not differ significantly between obese cows and controls. Whether this observed increase in ORO‐positive cells in the oviducts of obese cows affects their reproduction warrants further study.  相似文献   

A spatial model of population dynamics of the early life stages of Japanese sardine, Sardinops melanostictus, off the Pacific coast of Japan     

Maki Suda  Tatsu Kishida 《Fisheries Oceanography》2003,12(2):85-99

We constructed a numerical model reproducing the transport, survival and individual growth of the early life stages of Japanese sardine, Sardinops melanostictus, off the Pacific coast of Japan during 1978–93. The causes of early life stage mortality, including the influence of the effects of the spatial relationship between the spawning grounds and the Kuroshio on the mortality rate, were investigated. Survival and transport from egg stage to 60 days after spawning were modelled daily in a 1 × 1 degree mesh cell and individual growth in the period was modelled in each region (Kuroshio, Inshore, Offshore and Transition regions). Individual growth and survival from 60 to 180 days after spawning were modelled daily in the Transition region. Environmental data were taken from outside the model system. Our simulation indicates that survival variability in the larval stage (5–25 mm in standard length) is the key factor in determining the year‐class strength. The simulation revealed that strong year classes occurred with good survival in the spawning ground and whilst entrained in the Kuroshio current being transported to the main feeding grounds in the Transition region. The simulation also indicated that survival rates in 1988–93 were low in the Inshore, Kuroshio and Offshore regions, which depressed the year‐class strength during that period.  相似文献   

Distribution and seasonal activity variation of proteases in digestive tract of sea cucumber Stichopus japonicus     

Xueyan Fu  Changhu Xue  Benchun Miao  Zhaojie Li  Xin Gao  Takashi Hirata 《Fisheries Science》2006,72(5):1130-1132

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CO₂ concentration change in Nannochloropsis sp. culture medium     

Shigehisa Yamasaki  Hachiro Hirata 《Aquacultural Engineering》1995,14(4):357-365

CO₂ concentration in a marine phytoplankton, Nannochloropsis sp., culture medium was observed to consider whether or not CO₂ defficiency for growth occurs in the culture medium of a mass culture. In the first experiment, the Nannochloropsis sp. culture was continued for 4–5 days and the CO₂ concentration was observed three times a day, namely, the initial, middle and end of the light period. While, in the second experiment, the CO₂ concentration was observed every 2–3 hr for 24 hr on the second day after inoculation. In the first experiment, the total CO₂ concentration decreased gradually throughout the culture period, repeating its decrease and increase in the light and dark periods, respectively. At the end of the light period of the third day after inoculation, the CO₂ concentration decreased to concentrations which could not be determined. In the second experiment, the CO₂ concentration decreased in the light period, and increased in the dark period. The increment in the dark period was faster at heavier aeration. It was considered that some kind of carbon source should be supplied to obtain high density of Nannochloropsis sp. ealier under strong light intensity.  相似文献