Quantitative PCR detection of feline morbillivirus in cat urine samples     

Tetsuya FURUYA  Akiko WACHI  Yukiko SASSA  Tsutomu OMATSU  Makoto NAGAI  Ryuji FUKUSHIMA  Makoto SHIBUTANI  Tomohiro YAMAGUCHI  Yosuke UEMATSU  Kinji SHIROTA  Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1701-1703

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Seasonal changes in serum progesterone levels in Thoroughbred racehorses in training     

Yuji TAKAHASHI  Makoto AKAI  Harutaka MURASE  Yasuo NAMBO 《Journal of Equine Science》2015,26(4):135-139

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Effect of monensin withdrawal on rumen fermentation,methanogenesis and microbial populations in cattle          下载免费PDF全文

Arfan Abrar  Takamitsu Tsukahara  Makoto Kondo  Tomomi Ban‐Tokuda  Wang Chao  Hiroki Matsui 《Animal Science Journal》2015,86(9):849-854

This study was designed to obtain information on the residual influence of dietary monensin on ruminant fermentation, methanogenesis and bacterial population. Three ruminally cannulated crossbreed heifers (14 months old, 363 ± 11 kg) were fed Italian ryegrass straw and concentrate supplemented with monensin for 21 days before sampling. Rumen fluid samples were collected for analysis of short chain fatty acid (SCFA) profiles, monensin concentration, methanogens and rumen bacterial density. Post‐feeding rumen fluid was also collected to determine in vitro gas production. Monensin was eliminated from the rumen fluid within 3 days. The composition of SCFA varied after elimination of monensin, while total production of SCFA was 1.78 times higher than on the first day. Methane production increased 7 days after monensin administration ceased, whereas hydrogen production decreased. The methanogens and rumen bacterial copy numbers were unaffected by the withdrawal of monensin.  相似文献   

Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish     

Katsuki TANAKA  Hikaru ADACHI  Hironobu AKASAKA  Junya TAMAOKI  Yuji FUSE  Makoto KOBAYASHI  Takio KITAZAWA  Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1050

We reported the involvement of oxidative stress and prostaglandins including thromboxane and prostacyclin in pre-cardiac edema (early edema) caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While the involvement of oxidative stress in TCDD-induced toxicity has been frequently reported, the mechanism of its action is still unclear. In the present study, oxidative stress inducers including paraquat, hydrogen peroxide (H₂O₂) and rotenone augmented early edema (edema) induced by a low concentration of TCDD (0.1 ppb) at 55 hr post fertilization (hpf), while each of them alone did not cause edema. Edema caused by TCDD plus oxidative stress inducers was almost abolished by antioxidants, an antagonist for thromboxane receptor (ICI-192,605) and an agonist for prostacyclin receptor (beraprost), suggesting that the site of action of these inducers was in the regular signaling pathway after activation of aryl hydrocarbon receptor type 2 (AHR2) by TCDD. Oxidative stress inducers also enhanced edema caused by an agonist for the thromboxane receptor (U46619), and the enhancement was also inhibited by antioxidants. Sulforaphane and auranofin, activators of Nrf2 that is a master regulator of anti-oxidative response, did not affect U46619-evoked edema but almost abolished TCDD-induced edema and potentiation by paraquat in both TCDD- and U46619-induced edema. Taken together, the results suggest that oxidative stress augments pre-cardiac edema caused by TCDD via activation of thromboxane receptor-mediated signaling in developing zebrafish. As paraquat and other oxidative stress inducers used also are environmental pollutants, interaction between dioxin-like compounds and exogenous source of oxidative stress should also be considered.  相似文献   

Quantitative analysis of pattern of gonial proliferation during sexual maturation in Japanese scallop <Emphasis Type="Italic">Patinopecten yessoensis</Emphasis>     

Makoto Osada  Satoshi Nakamura  Akihiro Kijima 《Fisheries Science》2007,73(6):1318-1324

ABSTRACT:   This study was undertaken to describe and quantitatively analyze the pattern of development of gametes in the Japanese scallop Patinopecten yessoensis . Incorporation of 5-bromo-2'-deoxyuridine (BrdU) into gonial cells was used to quantitatively detect mitotically active gonial cells. Oogonia increased in number from November to December and decreased rapidly to March. A small number of oocytes was detected in November. Oocytes steadily increased in number and size up to March. The number of spermatogonia slightly increased from November to December, and increased markedly from January to March. Both ratios of BrdU-immunopositive gonial cells in the ovary and testis to gonial cells moderately increased from September to December. The ratio of BrdU-immunopositive spermatogonia to gonial cells drastically increased from January to February and kept an elevated level in March, whereas the oogonia started to disappear in January. The results suggest that the pattern of proliferation of gonial cells can be divided into two phases: (i) phase I, oogonia and spermatogonia slowly proliferate through the growing stage; and (ii) phase II, oogonia develop into oocytes and spermatogonia begin to proliferate rapidly through the mature and spawning stages. The proliferation of gonial cells is likely under different endocrine controls in phases I and II.  相似文献   

Mycobacteriosis in a Domestic Ferret (Mustela putorius furo)     

Makoto NAKATA  Yasutsugu MIWA  Masaya TSUBOI  Kazuyuki UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):705-709

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Distribution of free L-cysteine and glutathione in seaweeds   总被引：1，自引：0，他引：1  

Makoto Kakinuma  Chan Sun Park  Hideomi Amano 《Fisheries Science》2001,67(1):194-196

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Inhibitory effects of seaweeds on histamine release from rat basophile leukemia cells (RBL-2H3)     

Yoshimasa Sugiura  Yoshirou Takeuchi  Makoto Kakinuma  Hideomi Amano 《Fisheries Science》2006,72(6):1286-1291

ABSTRACT:   The effects of 80% methanol extracts from frozen samples of 41 macroalgae and one sea grass collected in the Ise-Shima region of Japan were investigated on histamine release from rat basophile leukemia cells (RBL-2H3) sensitized with antidinitrophenyl (DNP) IgE and stimulated with DNP-BSA. Of the 21 brown algae, five green and 15 red algae, and one sea grass tested, only extracts from seven brown algae suppressed histamine release from RBL cells, as determined by high-performance liquid chromatography (HPLC) analysis. When the cytotoxic effects of the seven brown algal extracts were investigated by Trypan blue staining, only Eisenia arborea and Sargassum thunbergii did not show cytotoxic effects. Therefore, we conclude that E. arborea and S. thunbergii may contain compounds that have antiallergic effects without inducing cell death.  相似文献   

Localization of Eimeripain,an Eimeria tenella Cathepsin B-Like Cysteine Protease,during Asexual and Sexual Intracellular Development in Chicken Ceca     

Makoto MATSUBAYASHI  Takeshi HATTA  Takeharu MIYOSHI  ANISUZZAMAN  Kazumi SASAI  Kayoko YAMAJI  Kameo SHIMURA  Takashi ISOBE  Kiyoshi KITA  Naotoshi TSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):531-537

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Effect of retinoic acid on gene expression profiles of bovine intramuscular preadipocytes during adipogenesis     

Yasushi Mizoguchi  Makoto Moriya  Daiki Taniguchi  Akira Hasegawa 《Animal Science Journal》2014,85(2):101-111

To investigate genes involved in intramuscular adipogenesis in ruminants, 16 genes with dramatic variable expression were selected. These were selected from the differentiation‐ and proliferation‐phase libraries of our previous serial analysis of gene expression (SAGE) studies of a clonal bovine intramuscular preadipocyte (BIP) cell line. We harvested the BIP cells over 12 days after adipogenic stimulation with all‐trans retinoic acid (ATRA). Quantitative real‐time PCR confirmed the earlier SAGE study results of the expression patterns of 15 of the genes. On day 6, TG accumulation increased significantly in the BIP cells but was completely inhibited in the 3T3‐L1 cells (the monogastric reference). ATRA enhanced expression levels of six genes whereas it suppressed expression of eight genes on day 3 of adipogenesis in the BIP cells. Forty‐eight hours after transfection, the messenger RNA expression level of the adipose differentiation‐related protein (ADFP), encoded by one of the upregulated genes, in the ADFP small interference RNA (siRNA)‐transfected cells was 3.5% of that in negative control‐transfected cells. Also, 6 days after induction the TG level in the ADFP siRNA‐transfected cells was 21.8% lower than that in negative control‐transfected cells. This analysis of gene expression profiles after ATRA treatment will contribute to our understanding of the molecular mechanisms involved in bovine intramuscular adipogenesis.  相似文献