Anti-erythrocyte membrane antibodies detected in sera of dogs naturally infected with Babesia gibsoni.     

K Adachi  A Yoshimoto  T Hasegawa  T Shimizu  Y Goto  S Makimura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1081-1084

Due to the potential for anti-erythrocyte membrane antibodies as possible enhancers of erythrocyte destruction, the presence of serum anti-erythrocyte membrane antibodies in 31 dogs with Babesia gibsoni infection admitted to a veterinary hospital was investigated by an enzyme linked immunosorbent assay (ELISA) and immunoblotting analyses. This infection resulted in an increase of anti-erythrocyte membrane antibodies in 84% (IgG) and 74% (IgM) of 31 infected dogs, respectively. This was confirmed by the similarity in the protein profiles of the erythrocyte membrane antigens immunoblotted with rabbit antiserum to dog erythrocyte membrane antigens and infected dog serum. These results suggest the production of anti-erythrocyte membrane antibodies was induced by B. gibsoni infection.  相似文献   

Theileria parva: the immune status of calves born of dams immunised against East Coast fever     

M P Cunningham  C G Brown  M J Burridge  S P Morzaria  G M Urquhart 《Research in veterinary science》1989,46(1):90-94

Eight Friesian cross cows three months pregnant to a single Friesian bull were immunised against East Coast fever by infection with Theileria parva (Muguga) sporozoite stabilate and treatment with pyrrolidino-methyl tetracycline. They were challenged with the homologous stock four times before calving and a fifth time after calving, and resisted all five challenges which killed all of the five groups of five susceptible controls. Calves born to these hyperimmunised dams were fully susceptible on challenge with the same stabilate, as were susceptible cows from the same farm and their calves. In both instances the calves died three to seven days earlier than the cows which were approximately 10 times heavier. These results show that one- to two-month-old taurine calves from artificially immunised dams are not protected from experimental T parva sporozoite challenge and that there is no inherent calfhood resistance to East Coast fever.  相似文献   

Laryngeal paralysis in immature and mature dogs as one sign of a more diffuse polyneuropathy     

K G Braund  H S Steinberg  A Shores  J E Steiss  J R Mehta  M Toivio-Kinnucan  K A Amling 《Journal of the American Veterinary Medical Association》1989,194(12):1735-1740

Six dogs with laryngeal paralysis had clinical, electrophysiologic, and pathologic evidence of a more generalized polyneuropathy. Three of the dogs were young Dalmatians, one was a young Bouvier des Flandres, and two were older, large-breed dogs. The results of this study suggest that laryngeal paralysis in dogs may frequently be one clinical sign of an underlying, more generalized polyneuropathy. Two forms of this generalized polyneuropathy may exist: an early form, as seen in young dogs with congenital or hereditary disease, and a delayed-onset form that is usually found in older dogs with so-called idiopathic laryngeal paralysis, some of which may have hypothyroidism.  相似文献   

The disposition of carbenicillin in blood,other body fluids and tissues of buffalo calves (Bubalus bubalis)     

K. S. Brar  M. S. Bal 《Veterinary research communications》1993,17(2):119-122

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Radiographic diagnosis of an expansile bone lesion in a dog     

C. R. Lamb  J. Berg  S. H. Schelling† 《The Journal of small animal practice》1993,34(5):239-241

An old dog had an expansile lesion affecting the ulnar diaphysis. The lesion had clinical and radiographic features typical of a bone cyst; however, computed X-ray tomography indicated that the lesion had a tissue content incompatible with a true cyst. The histological diagnosis was osteosarcoma. This report emphasises the highly variable radiological appearance of canine osteosarcoma; biopsy is required to establish the diagnosis because the radiological signs may mimic a lesion of different aetiology.  相似文献   

Thrombocyte aggregation in the cat     

S Hart  I Nolte 《Tier?rztliche Praxis》1991,19(4):413-418

Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation.  相似文献   

Feline Ubiquitin Fusion Protein Genes     

Kano  R.  Kubota  A.  Nakamura  Y.  Watanabe  S.  Hasegawa  A. 《Veterinary research communications》2001,25(8):615-622

Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells.  相似文献   

Effect of energy source and xylanase addition on energy metabolism, performance, chemical body composition and total body electrical conductivity (TOBEC) of broilers   总被引：3，自引：0，他引：3  

S. Dänicke  I. Halle  E. Strobel  E. Franke  & H. Jeroch 《Journal of animal physiology and animal nutrition》2001,85(9-10):301-313

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Valvular pulmonic stenosis in bullmastiffs     

R. Malik  D. B. Church  G. B. Hunt 《The Journal of small animal practice》1993,34(6):288-292

Six bullmastiffs with congenital heart disease were evaluated over a three-year period. In all the cases the problem was shown to be dysplasia of the pulmonary valve. Five cases were male, one was female. All dogs available to follow-up developed signs of cardiac decompensation. Bullmastiffs must be added to the list of breeds in which valvular pulmonic stenosis is encountered frequently.  相似文献   

Measurement of nitrate leaching losses from arable plots under different nitrogen input regimes     

A.J.A. Vinten  R.S. Howard  M.H. Redman 《Soil Use and Management》1991,7(1):3-13

Abstract. Leaching losses of nitrate-nitrogen were measured from a set of eight hydrologically isolated plots on a clay loam soil over the period from September 1987 to February 1990. Variable drainflow recovery from the plots hampered accurate estimation of nitrate loading, but results suggest that, when inorganic nitrogen fertilizer is applied up to the recommended amount, there is little influence of the amount applied on the amount leached. We did, however, observe the following effects on nitrate leaching: leguminous green manure incorporated in autumn increased leaching of nitrate-nitrogen by 10–15 kg per hectare during the winter; autumn cultivation caused some increase in leaching compared with no cultivation in one year; some systematic variations in nitrate leaching occurred between years and between plots, but were unrelated to treatments.
From the results we conclude that green manuring does not provide sufficient nitrogen for organically grown crops on this soil but contributes significantly to nitrate leaching, and that growing spring cereals, with the land remaining in stubble as long as possible in autumn, may be the best strategy to minimize nitrate leaching.  相似文献