Lipid peroxidation-derived hepatotoxic aldehydes, 4-hydroxy-2E-hexenal in smoked fish meat products   总被引：1，自引：0，他引：1  

DEEPTHI MS  MUNASINGHE  KEN-ICHIRO  ICHIMARU  MIYO  RYUNO  NOBUHIKO  UEKI  TAKANAO  MATSUI  KAZUHIRO  SUGAMOTO  SATOSHI  KAWAHARA  TADASHI  SAKAI 《Fisheries Science》2003,69(1):189-194

ABSTRACT:   Contents of 4-hydroxy-2 E -hexenal (HHE), hepatotoxic aldehydes, in smoked fish meat products (smoked salmon and fish meat sausage) were analyzed. Large differences in these contents between the different samples were observed. Very low levels of HHE were detected in fish meat sausage samples. However, a high level of HHE was observed in one batch of smoked salmon. Changes of HHE contents in yellowtail meat containing cherry and sugi wood vinegar stored at 0°C were also analyzed for 7 days. Malonaldehyde (MA) was also analyzed in these samples as an index of the lipid peroxidation level. After 3 or 7 days of storage, HHE contents in both wood vinegar-added samples were significantly higher, but MA contents were significantly lower than those of the control.  相似文献   

Changes of proximate compositions and myoglobin content in the dorsal ordinary muscles of the cultured Pacific bluefin tuna <Emphasis Type="Italic">Thunnus orientalis</Emphasis> with growth     

Yoshi -Nori Nakamura  Masashi Ando  Manabu Seoka  Ken-ichi Kawasaki  Yasuyuki Tsukamasa 《Fisheries Science》2007,73(5):1155-1159

ABSTRACT:   Using the dorsal ordinary muscle (DOM) of cultured Pacific bluefin tuna (body length [BL]: 47.5–81.8 cm, body weight [BW]: 2.1–13.5 kg, n  = 15), the changes of proximate compositions and myoglobin (Mb) content with growth were investigated. There was a positive correlation ( r  = 0.9832, P  < 0.05) of BL and BW in cultured tuna. The protein contents of the DOM of cultured tuna decreased ( P  < 0.05) and the lipid contents had a tendency to increase (not significantly) with growth. The meat color changed from pink to red with growth. In addition, the Mb contents of the DOM of cultured tuna increased ( P  < 0.05) from 1.0 mg/g (minimum BW fish) to 3.8 mg/g (maximum BW fish) with growth. These results indicate that the increase of the Mb content in the DOM of cultured tuna is not caused by the restriction of exercise and overfeeding between 2.1 kg and 13.5 kg of BW.  相似文献   

Toxicity and immunogenicity of Aeromonas salmonicida extracellular products to salmonids     

E. KAWAHARA  S. OSHIMA  S. NOMURA 《Journal of fish diseases》1990,13(6):495-503

Abstract. Aeromonas salmonicida extracellular products (ECP) were fractionated by gel filtration chromatography. Three protein fractions were found. The first and second fractions showed low and high haemolytic activity, respectively, whereas the third fraction showed protcolytic activity. By intramuscular injection into juvenile rainbow trout, the median lethal doses of the first, second and third fractions were calculated at >669, 152 and <1514μg/g body weight, respectively. Cytopathic effects of the fractionated ECP to coho salmon lymphocytes were observed in vitro. The first and second fractions caused cell deformation, nuclear granulation and cytoplasmic streaming after 3h. No cytologic effects with the third fraction were observed. A mixture of the first and third fractions, a mixture of the second and third fractions, and non-fractionated ECP caused nuclear granulation and cytoplasmic streaming after 3Qmin. Using immunodiffusion analysis, the first and second fractions formed a single precipitating line each against white-spotted char anti-ECP sera, but the third fraction did not formed a precipitating line against the antisera.  相似文献   

Influence of different dietary amino acid patterns on growth and body composition of juvenile Japanese flounder, Paralichthys olivaceus     

. Shah Alam  Shin-ichi Teshima  Dedy Yaniharto  Shunsuke Koshio  Manabu Ishikawa 《Aquaculture (Amsterdam, Netherlands)》2002,210(1-4):359-369

A feeding trial using five semi-purified diets (50% crude protein) was conducted to investigate the effects of different dietary amino acid patterns on growth and body composition of juvenile Japanese flounder. The control diet contained casein and gelatin as intact protein sources and four other diets contained 30% casein–gelatin (2:1, w/w) and 20% crystalline amino acids (CAA). CAA were added to the diets to simulate the amino acid pattern found in red sea bream egg protein (REP), Japanese flounder larvae whole body protein (FLP), Japanese flounder juvenile whole body protein (FJP), and brown fish meal protein (BFP), respectively. The test diets were fed to triplicate groups of juveniles (2.75±0.05 g) twice a day for 40 days to evaluate weight gain, survival, feed conversion efficiency (FCE), protein efficiency ratio (PER), and apparent protein utilization (APU). The apparent retention of total dietary amino acids in the whole body and A/E ratios of the whole body were also evaluated. The highest weight gain was observed in fish fed the diet containing the dietary amino acid pattern of BFP followed by fish fed the control, FJP, FLP and the REP diets. Percent survival, FCE, PER and APU were also significantly (P<0.05) affected by the amino acid pattern in the diets, indicating the highest value in fish fed the BFP diet. Except for a few amino acids, the amino acid composition of the whole body did not show marked differences with different dietary amino acid pattern. Results suggest that BFP could be more suitable as a reference amino acid pattern in the diet of juvenile Japanese flounder compared to the amino acid pattern of FLP, FJP or REP.  相似文献   

Comparison of apparent digestibility coefficient among replicates and different stocking density in red sea bream <Emphasis Type="Italic">Pagrus major</Emphasis>     

Amal K Biswas  Manabu Seoka  Kenji Takii  Hidemi Kumai 《Fisheries Science》2007,73(1):19-26

Two consecutive feeding trials were conducted to know: (i) how the apparent digestibility coefficient (ADC) varies among replicates on different days and during the course of the experiment (Trial 1); and (ii) the effect of stocking density (e.g. 7.17, 5.56, and 3.92 kg fish/t water) on the ADC of nutrients and energy in red sea bream (Trial 2). In both trials, 0.5% Cr₂O₃ was used as an inert marker. In Trial 1, there were no significant differences in ADC within the replicates although the values on different days varied significantly during the second week. The ADC of all parameters in the third and fourth weeks was significantly higher than in the second week (P<0.05). Although statistically insignificant, the lower stocking densities (3.92 and 5.56 kg fish/t water) showed better growth performance and superior ADC of protein, lipid, and energy than the higher stocking density in Trial 2. The results indicated that it may be better to acclimate fish to the diet for at least three weeks to obtain more reliable data by using a single tank for each treatment for digestibility experiments. Results also indicated that growth and digestibility performance can be stimulated by controlling stocking density.  相似文献   

An H5N1 highly pathogenic avian influenza virus that invaded Japan through waterfowl migration     

Kajihara M  Matsuno K  Simulundu E  Muramatsu M  Noyori O  Manzoor R  Nakayama E  Igarashi M  Tomabechi D  Yoshida R  Okamatsu M  Sakoda Y  Ito K  Kida H  Takada A 《The Japanese journal of veterinary research》2011,59(2-3):89-100

In 2010, an H5N1 highly pathogenic avian influenza virus (HPAIV) was isolated from feces of apparently healthy ducks migrating southward in Hokkaido, the northernmost prefecture of Japan. The H5N1 HPAIVs were subsequently detected in domestic and wild birds at multiple sites corresponding to the flyway of the waterfowl having stopovers in the Japanese archipelago. The Hokkaido isolate was genetically nearly identical to H5N1 HPAIVs isolated from swans in the spring of 2009 and 2010 in Mongolia, but less pathogenic in experimentally infected ducks than the 2009 Mongolian isolate. These findings suggest that H5N1 HPAIVs with relatively mild pathogenicity might be selected and harbored in the waterfowl population during the 2009-2010 migration seasons. Our data provide "early warning" signals for preparedness against the unprecedented situation in which the waterfowl reservoirs serve as perpetual sources and disseminators of HPAIVs.  相似文献   

Trehalose may prevent postsurgical adhesions in a rabbit model of hysterotomy     

Fujino H  Lee S  Suzuki S  Chung UI  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):931-935

The adhesion-preventing effect of trehalose in visceral organs was evaluated in a rabbit model. All rabbits underwent a hysterotomy, during which mild abrasion and desiccation was induced on the surfaces of visceral organs. In the control group, the intestines were kept dry during hysterotomy. In the saline and trehalose groups, saline solution and 7% trehalose solution, respectively, were sprayed on the organ surfaces. Adhesion formation on organ surfaces on which abrasion and desiccation were induced declined significantly in the trehalose group compared with the other groups. In contrast, at the hysterotomy site, there was no significant difference in adhesions between groups, suggesting that trehalose prevents adhesions in organs that have developed minor inflammation by abrasion and desiccation.  相似文献   

Screening of therapeutic targets for canine mast cell tumors from a variety of kinase molecules     

Takeuchi Y  Fujino Y  Watanabe M  Nakagawa T  Ohno K  Sasaki N  Sugano S  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1295-1302

Options of systemic treatment for canine MCT have been still limited and most canine cases with MCTs eventually undergo relapses even after achievement of a remission. Thus additional therapies are required to establish for the tumor. To identify the novel candidate therapeutic targets for canine MCT, the mRNA expression and phosphorylation statuses of several receptor or non-receptor kinases as well as the inhibitory effect of 95 specific inhibitors on the growth were assessed in three canine MCT cell lines (HRMC, VIMC1 and CMMC1). Among the 14 targets, the mRNAs of 11, 7 and 7 kinases were amplified in HRMC, VIMC1 and CMMC1, respectively. The mRNAs of VEGFR3, PDGFRα, SRC, YES, LCK and FYN were detected in all cell lines. The phosphorylation of 12, 8 and 7 kinases was observed by using specific antibody arrays in HRMC, VIMC1 and CMMC1, respectively. DTK, EPHB6, AMPKα1, CREB, STAT5a and STAT5b were phosphorylated in all cell lines. The 10, 9 and 17 inhibitors exhibited the biological activity against the growth of HRMC, VIMC1 and CMMC1, respectively. Only three inhibitors such as SB218078 (for Chk1), PDGF RTK inhibitor IV (for PDGFR) and radicicol (for Hsp90) suppressed the growth of all three cell lines. The present study indicated that several kinases, such as Chk1, PDGFR and Hsp90, could be used as therapeutic targets in the treatment for canine MCT. Further studies and clinical trials are warranted to apply the inhibitors for the treatment of the tumor.  相似文献   

Direct detection of equine herpesvirus type 1 DNA in nasal swabs by loop-mediated isothermal amplification (LAMP)     

Nemoto M  Ohta M  Tsujimura K  Bannai H  Yamanaka T  Kondo T  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1225-1227

We evaluated loop-mediated isothermal amplification (LAMP) as a means of detecting equine herpesvirus type 1 (EHV-1) DNA directly from nasal swabs. To increase the sensitivity, we added a step in which the samples were heat-treated to the original LAMP procedure. The detection limit of the LAMP assay with heat treatment was 10 times more sensitive than the original LAMP assay even when the DNA extraction step was omitted. In addition, the LAMP assay with heat treatment was more sensitive than the original LAMP assay and the polymerase chain reaction using clinical samples. The LAMP assay with heat treatment is easy to perform and so should be applicable to the diagnosis of EHV-1 infections in clinical laboratories.  相似文献   

Effects of Dietary Vitamin A on Juvenile Red Sea Bream Chrysophrys major     

Luis  Hector Hernandez  Hernandez  Shin-Ichi  Teshima  Manabu  Ishikawa Shunsuke  Koshio Yoshito  Tanaka 《Journal of the World Aquaculture Society》2004,35(4):436-444

A 55-d feeding experiment was conducted to evaluate the effects of dietary vitamin A on growth of juvenile red sea bream Chrysophrys major . Nine semi-purified diets with supplemental retinol palmitate at levels of 0, 300, 600, 1,500, 3,000, 4,500, 6,000, 15,000 and 30,000 retinol eq/kg diet, were fed twice daily to triplicate groups of 10 juveniles per tank with an initial weight of 1.178 ± 0.001 g. Weight gain and specific growth rate increased with the increase of supplemental retinol palmitate in the diet up to a level of 6,000 retinol eq/kg diet, beyond which those indices tended to decrease slightly or to plateau. Significantly lower weight gain and specific growth rate were observed in fish fed with 0 and 300 retinol eq/kg diet. No significant differences were found in survival rates among the different levels of supplementation. The crude protein, lipid, moisture and ash contents in the whole body were not affected by the different vitamin A supplemental levels. Besides the reduced growth, fish fed with low levels of vitamin A supplement showed no other signs of deficiency. No hypervitaminosis A symptoms were observed in fish fed on high levels of supplementation. Fish fed on diets with 0 and 300 retinol eq/kg diet showed a significantly lower content of vitamin A (as a total retinol) in the liver than those in groups fed with higher levels of supplementation. Vitamin A was stored in the liver mainly as retinyl esters. Total retinal content of the eye showed no significant differences among the treatments; however, fish fed with 0 and 300 retinol eq/kg of diet had slightly higher concentrations. According to the results of the mean weight gain, specific growth rate and liver retinol content, the requirement of dietary vitamin A for juvenile red sea bream was estimated to be between 1,500 and 6,000 retinol eq/kg.  相似文献