Colitis associated with Clostridium difficile in specific-pathogen-free C3H-scid mice     

Kawano A  Ikeda M  Iritani R  Kinoshita A  Watanabe K  Hayao T  Kokubo T  Matsushita S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):973-975

Soft feces and a decreased delivery rate were observed in a specific-pathogen-free (SPF) C3H-scid mouse breeding colony. Grossly, the ceca were shrunken and edematous in the affected mice. Histopathologically, severe edema in the cecal submucosa as well as infiltration of inflammatory cells in the lamina propria and submucosa of the ceca and colon were observed. No pathogenic microorganisms were detected by the routine microbiological tests. By anaerobic bacterial-examination, Clostridium (C.) difficile with toxin A was isolated from the cecal contents of the affected mice. The mice were diagnosed with C. difficile-associated colitis. This case appears to be the first report of natural infection with C. difficile in SPF mice with clinical signs.  相似文献   

Efficacy of five commercial disinfectants and one anionic surfactant against equine herpesvirus type 1     

Koji TSUJIMURA  Harutaka MURASE  Hiroshi BANNAI  Manabu NEMOTO  Takashi YAMANAKA  Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1545-1548

We investigated the influences of various reaction conditions on equine herpesvirus type 1 (EHV-1) disinfection by 5 commercial disinfectants (3 quaternary ammonium compounds [QACs] and 2 chlorine-based disinfectants) and 1 anionic surfactant. QACs at their highest recommended concentrations had no virucidal effect on EHV-1 with a 10-min reaction time at 0°C or a 1-min reaction time at room temperature. Chlorine-based disinfectants achieved EHV-1 disinfection with a 10-min reaction time at −10°C or a 30-sec reaction time at room temperature. In the presence of 5% fetal bovine serum, QACs (except for benzalkonium chloride) showed more stable virucidal effects than did chlorine-based disinfectants. The virucidal effect of the anionic surfactant was almost equivalent to that of the QACs.  相似文献   

Melatonin protects the integrity of granulosa cells by reducing oxidative stress in nuclei,mitochondria, and plasma membranes in mice     

Manabu TANABE  Hiroshi TAMURA  Toshiaki TAKETANI  Maki OKADA  Lifa LEE  Isao TAMURA  Ryo MAEKAWA  Hiromi ASADA  Yoshiaki YAMAGATA  Norihiro SUGINO 《The Journal of reproduction and development》2015,61(1):35-41

Melatonin protects luteinized granulosa cells (GCs) from oxidative stress in the follicle during ovulation. However, it is unclear in which cellular components (e.g., nuclei, mitochondria, or plasma membranes) melatonin works as an antioxidant. GCs from immature (3 wks) ICR mice were incubated with hydrogen peroxide (H2O2; 0.01, 0.1, 1, 10 mM) in the presence or absence of melatonin (100 μg/ml) for 2 h. DNA damage was assessed by fluorescence-based immunocytochemistry using specific antibodies for 8-hydroxydeoxyguanosine (8-OHdG), an indicator of oxidative guanine base damage in DNA, and for histone H2AX phosphorylation (γH2AX), a marker of double-strand breaks of DNA. Mitochondrial function was assessed by the fluorescence intensity of MitoTracker Red probes, which diffuse across the membrane and accumulate in mitochondria with active membrane potentials. Lipid peroxidation of plasma membranes was analyzed by measuring hexanoyl-lysine (HEL), a oxidative stress marker for lipid peroxidation. Apoptosis of GCs was assessed by nuclear fragmentation using DAPI staining, and apoptotic activities were evaluated by caspase-3/7 activities. H2O2 treatment significantly increased the fluorescence intensities of 8-OHdG and γH2AX, reduced the intensity of MitoTracker Red in the mitochondria, increased HEL concentrations in GCs, and enhanced the number of apoptotic cells and caspase-3/7 activities. All these changes were significantly decreased by melatonin treatment. Melatonin reduced oxidative stress-induced DNA damage, mitochondrial dysfunction, lipid peroxidation, and apoptosis in GCs, suggesting that melatonin protects GCs by reducing oxidative stress of cellular components including nuclei, mitochondria, and plasma membranes. Melatonin helps to maintain the integrity of GCs as an antioxidant in the preovulatory follicle.  相似文献   

Cesium radioactivity in peripheral blood is linearly correlated to that in skeletal muscle: Analyses of cattle within the evacuation zone of the Fukushima Daiichi Nuclear Power Plant          下载免费PDF全文

Tomokazu Fukuda  Yasushi Kino  Yasuyuki Abe  Hideaki Yamashiro  Jin Kobayashi  Yoshinaka Shimizu  Atsushi Takahashi  Toshihiko Suzuki  Mirei Chiba  Shintaro Takahashi  Kazuya Inoue  Yoshikazu Kuwahara  Motoko Morimoto  Hisashi Shinoda  Masahiro Hiji  Tsutomu Sekine  Manabu Fukumoto  Emiko Isogai 《Animal Science Journal》2015,86(1):120-124

The accident at the Fukushima Daiichi Nuclear Power Plant (FNPP) released a large amount of radioactive substances into the environment. Furthermore, beef contaminated with radioactive cesium above the 500 Bq/kg safety standard was circulated in the food chain in 2011. Japanese consumers remain concerned about the safety of radioactively contaminated food. In our previous study, we detected a linear correlation between radioactive cesium (¹³⁷Cs) activity in blood and muscle around 500 to 2500 Bq/kg in cattle. However, it was unclear whether the correlation was maintained at a lower radioactivity close to the current safety standard of 100 Bq/kg. In this study, we evaluated 17 cattle in the FNPP evacuation zone that had a ¹³⁷Cs blood level less than 10 Bq/kg. The results showed a linear correlation between blood ¹³⁷Cs and muscle ¹³⁷Cs (Y = 28.0X, R² = 0.590) at low radioactivity concentration, indicating that cesium radioactivity in the muscle can be estimated from blood radioactivity. This technique would be useful in detecting high‐risk cattle before they enter the market, and will contribute to food safety.  相似文献   

Growth properties and immunogenicity of a virus generated by reverse genetics for an inactivated equine influenza vaccine     

Minoru Ohta  Hiroshi Bannai  Yoshinori Kambayashi  Norihisa Tamura  Koji Tsujimura  Seiya Yamayoshi  Yoshihiro Kawaoka  Manabu Nemoto 《Equine veterinary journal》2022,54(1):139-144

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The formation process of button ulcers in pigs experimentally infected with a subgenotype 2.1 isolate of classical swine fever virus     

Kasumi SUDO  Manabu YAMADA  Mariko OCHIAI  Shoko IWAMOTO  Hajime SAKAKI  Chiho KUSHIDA  Maiko YAMASHITA  Atsushi YAMAMOTO  Takao NAGASAKA  Mikio OIDEMIZU  Akito SAITO  Kinya YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):990

We evaluated the role of classical swine fever virus (CSFV) in the formation of button ulcers in the mucosa of the gastrointestinal tract. Histopathological and immunohistochemical analyses of pigs experimentally infected with a subgenotype 2.1 isolate of CSFV, which was isolated in Japan in 2019, revealed follicular necrosis in the submucosal mucosa-associated lymphoid tissue and herniation of crypts as factors that contribute to the development of button ulcers during CSFV infection. These findings indicate that CSFV induces follicular necrosis and is one of the causative agents of button ulcers in pigs.  相似文献   

Dermal Cell Damage Induced by Topical Application of Non-Steroidal Anti-Inflammatory Drugs is Suppressed by Trehalose Co-Lyophilization in Ex Vivo Analysis     

Yuko KAYASUGA-KARIYA  Shintaroh IWANAGA  Ayano FUJISAWA  Lee-Shuan LIN  Shigeki SUZUKI  Ung-il CHUNG  Nobuo SASAKI  Nobuyuki SHIMOHATA  Manabu MOCHIZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1619-1622

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Improving a Complement-fixation Test for Equine Herpesvirus Type-1 by Pretreating Sera with Potassium Periodate to Reduce Non-specific Hemolysis     

Hiroshi BANNAI  Manabu NEMOTO  Koji TSUJIMURA  Takashi YAMANAKA  Takashi KONDO  Tomio MATSUMURA 《Journal of Equine Science》2013,24(4):71-74

Non-specific hemolysis has often been observed during complement-fixation (CF) tests for equine herpesvirus type-1 (EHV-1), even when the sera have virus-specific CF antibodies. This phenomenon has also been reported in CF tests for various infectious diseases of swine. We found that the sera from 22 of 85 field horses (25.9%) showed non-specific hemolysis during conventional CF testing for EHV-1. Because pretreatment of swine sera with potassium periodate (KIO₄) improves the CF test for swine influenza, we applied this method to horse sera. As we expected, horse sera treated with KIO₄ did not show non-specific hemolysis in the EHV-1 CF test, and precise determination of titers was achieved.  相似文献   

Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration     

Manabu NEMOTO  Keiichiro KIZAKI  Yoshio YAMAMOTO  Toshina OONUMA  Kazuyoshi HASHIZUME 《Journal of Equine Science》2013,24(2):17-24

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Promotion of initial swimbladder inflation in Pacific bluefin tuna,Thunnus orientalis (Temminck and Schlegel), larvae     

Michio Kurata  Manabu Seoka  Yoshizumi Nakagawa  Yasunori Ishibashi  Hidemi Kumai  Yoshifumi Sawada 《Aquaculture Research》2012,43(9):1296-1305

Initial swimbladder inflation (ISI) of Pacific bluefin tuna (PBT), Thunnus orientalis, larvae was studied to increase the survival of cultured larvae. Experiment 1 was conducted to explore promotion and inhibition of ISI under different water surface conditions; including the use of surface skimmer to remove autogenous surface substances (SS), covering the water surface with liquid‐paraffin‐layer (LP) and oil film (OF), and a control (non‐treatment, NT). Significantly higher inflation frequency was observed in SS (62.2%) than NT (11.9%), LP (2.7%) and OF (3.9%). This indicates that ISI in PBT larvae can be promoted by removal of surface substances on rearing water which inhibit larval air gulping. Experiment 2 aimed to elucidate proper day of larval age to start skimming for promoting ISI with four different periods of oil film removal: from 3 to 8 (SF3D), 4 to 8 (SF4D), 5 to 8 (SF5D), 6 to 8 (SF6D) days‐post‐hatch (dph). Significant improvement in ISI frequency was observed in SF3D (80.2%) but the frequency was very poor in SF4D, SF5D, and SF6D (17.8–7.5%). This implies the need of oil film removal without missing a narrow window, 1 day of 3 dph, to promote ISI in practical PBT larviculture.  相似文献