THORACOLUMBAR INTRADURAL DISC HERNIATION IN EIGHT DOGS: CLINICAL,LOW‐FIELD MAGNETIC RESONANCE IMAGING,AND COMPUTED TOMOGRAPHIC MYELOGRAPHY FINDINGS          下载免费PDF全文

Shinji Tamura  Shoko Doi  Yumiko Tamura  Kuniaki Takahashi  Hirokazu Enomoto  Tsuyoshi Ozawa  Kazuyuki Uchida 《Veterinary radiology & ultrasound》2015,56(2):160-167

Intradural disc herniation is a rarely reported cause of neurologic deficits in dogs and few published studies have described comparative imaging characteristics. The purpose of this retrospective cross sectional study was to describe clinical and imaging findings in a group of dogs with confirmed thoracolumbar intradural disc herniation. Included dogs were referred to one of four clinics, had acute mono/paraparesis or paraplegia, had low field magnetic resonance imaging (MRI) and/or computed tomographic myelography, and were diagnosed with thoracolumbar intradural disc herniation during surgery. Eight dogs met inclusion criteria. The prevalence of thoracolumbar intradural disc herniation amongst the total population of dogs that developed a thoracolumbar intervertebral disc herniation and that were treated with a surgical procedure was 0.5%. Five dogs were examined using low‐field MRI. Lesions that were suspected to be intervertebral disc herniations were observed; however, there were no specific findings indicating that the nucleus pulposus had penetrated into the subarachnoid space or into the spinal cord parenchyma. Thus, the dogs were misdiagnosed as having a conventional intervertebral disc herniation. An intradural extramedullary disc herniation (three cases) or intramedullary disc herniation (two cases) was confirmed during surgery. By using computed tomographic myelography (CTM) for the remaining three dogs, an intradural extramedullary mass surrounded by an accumulation of contrast medium was observed and confirmed during surgery. Findings from this small sample of eight dogs indicated that CTM may be more sensitive for diagnosing canine thoracolumbar intradural disc herniation than low‐field MRI.  相似文献   

Correlation of minimum inhibitory concentrations between human and animal antimicrobials against Escherichia coli isolated from livestock     

Manao Ozawa  Michiko Kawanishi  Mariko Uchiyama  Daisuke Mitsuya  Hitoshi Abo  Ryoji Koike  Mayumi Kijima 《Journal of veterinary diagnostic investigation》2021,33(4):744

We analyzed the correlation between minimum inhibitory concentrations (MICs) of antimicrobials used in humans and those used in animals to enable comparison of antimicrobial susceptibility between Escherichia coli isolated from humans and those from animals. We compared the following pairs of MIC data: piperacillin (PIPC) to ampicillin (ABPC), amikacin (AMK) to kanamycin (KM), minocycline (MINO) to oxytetracycline (OTC), and levofloxacin (LVFX) to enrofloxacin (ERFX) using 103 isolates of E. coli from healthy livestock (cattle, pigs, broiler chickens, and layer chickens). Kappa analysis of the agreement for resistance and susceptibility between PIPC and ABPC, AMK and KM, MINO and OTC, and LVFX and ERFX showed almost perfect (κ = 0.81), slight (κ = 0.12), fair (κ = 0.37), and moderate (κ = 0.46) agreement, respectively. Within the antimicrobial pairs, all isolates resistant to the human antimicrobial were also resistant to the veterinary antimicrobial. However, there was less agreement within the pairs for those isolates that were sensitive to the human antimicrobial. The percentage agreement for susceptibility, defined as the percentage of isolates sensitive to both antimicrobials compared with isolates sensitive to both antimicrobials, as well as those sensitive only to the human antimicrobial, was 89.9%, 87.3%, 64.0%, and 89.9% for PIPC and ABPC, AMK and KM, MINO and OTC, and LVFX and ERFX, respectively. Our results suggest that the possibility of missing the resistance for antimicrobials used in human medicine by examining MICs for the equivalent antimicrobials used in veterinary medicine is low.  相似文献   

Characterization of sago palm (Metroxylon sagu) lignin by analytical pyrolysis     

Kuroda K  Ozawa T  Ueno T 《Journal of agricultural and food chemistry》2001,49(4):1840-1847

Dioxane lignin prepared from sago palm (Metroxylon sagu) was characterized by analytical pyrolysis coupled to gas chromatography-mass spectrometry. Large abundances of the p-hydroxybenzoates ester-linked to the lignin were proven by analytical pyrolysis as well as by mild alkaline treatment that produced p-hydroxybenzoic acid in 16.3% yield. Pyrolysis in the presence of tetramethylammonium hydroxide (TMAH) before and after alkaline treatment also showed the presence of ester- and ether-linked p-hydroxybenzoates. Quantitative results of pyrolysis showed that the sago palm lignin is of syringyl type. The relative abundances of TMAH/pyrolysis products derived from the syringyl beta-aryl ether substructures were 4.9 times those of the guaiacyl equivalents. Proton nuclear magnetic resonance analysis also showed the presence of the p-hydroxybenzoates and the predominance of the syringyl moiety over the guaiacyl ones in the sago palm lignin.  相似文献   

Influence of thinning on the shelter effect of windbreaks as clarified by a wind tunnel experiment     

Hajime Ozawa  Tomoki Sakamoto  Hiroaki Hagino 《Journal of Forest Research》2007,12(3):222-229

We measured the shelter effect of model windbreaks in a wind tunnel before and after adjustment thinning based on methods for managing overcrowded windbreaks. One of the aims of this experiment was to determine whether or not the actual thinning done on an actual windbreak would sustain the shelter effect. The shelter effects were evaluated based on the critical wind speed and the shelter distance. Six model windbreaks were set to model a windbreak after the thinning (AT1–6) and one was set to model it before the thinning (BT). In models AT1–3, the trees in the middle part of the windbreak were cut and the trees in both the windward and leeward parts were left. AT1 and AT2 were models that reproduced the actual windbreak after the thinning. In the other three models (AT4–6), which were set as additional thinning models based on AT3, trees in both the windward and leeward parts were cut. The thinning ratios of these models ranged from 21% (AT1) to 84% (AT6). The shelter effects of AT1–3 were sustained or only slightly decreased compared with that of BT. The shelter effects of AT4 (thinning ratio of 63%) and AT5 (79%) were the highest among all of the models. From these results, it was confirmed that the thinning done on an actual windbreak was able to sustain the windbreak’s shelter effect. If the trees at the edges are not felled, the shelter effect of the windbreak can be sustained or improved, even if the thinning is heavier than AT2.  相似文献   

新疆绿洲农业中新节水栽培方法的探讨     

杨戈  古丽努尔 《干旱区研究》1996,13(1):24-31

尽管新疆水资源是有限的，每年仍有大量的朋用于作物的生产。为了节约用水，我们于１９９５年在中国科学院阜康荒漠生态系统观测试验站进行了研究试验，并得到如下结果。  相似文献   

Antigenic types of bluetongue virus prevalent in Egypt     

S. M. Hafez  Y. Ozawa 《Tropical animal health and production》1981,13(1):49-54

Summary In an attempt to identify the antigenic types of bluetongue (BT) virus prevalent in Egypt 31 selected sheep sera from different parts of Egypt which had been screened by gel precipitation tests were examined by a plaque reduction test using 16 types of BT virus. Dilutions 1:5 of those screened sera reduced more than 50% of plaques of at least 1 of 16 types of BT virus. Two sera completely inhibited plaque formation with type 4 virus, 1 with type 13, another serum with type 15 and 2 other sera with type 16 virus. The significance of the above serological findings is discussed.

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Tipos Antigenicos Del Virus De Lengua Azul En Egipto

Resumen Se examinaron 31 sueros seleccionados de ovejas de diferentes partes de Egipto. La prueba utilizada fué la de reducción de placas usando 16 tipos de virus de lengua azul. Los sueros habían sido examinados previamente mediante la prueba de precipitación en agar. Las diluciones 1:5 de los sueros examinados previamente, redujeron más del 50% de placas de por lo menos 1 de 16 tipos de virus de lengua azul. Dos de los sueros inhibieron completamente la formación de placas con el virus tipo 4, 1 con el tipo 13, otro suero con el tipo 15 y dos más con el tipo 16. Se discute el resultado de las pruebas serológicas.

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Types D'Antigenes Du Virus De La Blue Tongue Existant En Egypte

Résumé Pour identifier les types antigéniques du virus de la Blue Tongue existant en Egypte, 31 sérums de mouton provenant de diverses régions du pays, préalablement examinés par immunoprécipitation en milieu gélifié, ont été soumis à un test de réduction des plages avec 16 sérotypes du virus. Les dilutions au 1/5 de ces sérums ont réduit les plages de plus de 50 p. 100 pour au moins un des 16 sérotypes.Deux sérums ont complétement inhibé la formation des plages avec le type 14, avec le type 13, un autre avec le type 15 et deux enfin avec le type 16.La signification de ces résultats sérologiques est discutée.

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Mercaptan-capturing properties of mushrooms.   总被引：2，自引：0，他引：2  

O Negishi  Y Negishi  Y Aoyagi  T Sugahara  T Ozawa 《Journal of agricultural and food chemistry》2001,49(11):5509-5514

Mercaptan-capturing properties of 33 kinds of mushrooms were measured. The mushrooms having a high capturing ability toward methyl mercaptan (MeSH) were Agaricus bisporus, A. campestris, Boletus fraternus, B. subvelutipes, Gyrodon lividus, Leccinum scabrum, Suillus grevillei, Morchella esculenta, Russula nigricans, Hypholoma sublateritium, and Lyophyllum sykosporum. These are liable to change their color when injured. The mixture of their acetone powders, which contain polyphenol oxidases, and phenolic compounds such as tyrosine, gamma-L-glutaminyl-4-hydroxybenzene (GHB), DOPA, variegatic acid, grevillin B and C, and pigments, and fluorescent compounds from H. sublateritium also showed high MeSH-capturing properties. 2,5-Bis(methylthio)-DOPA was isolated from the reaction mixture of tyrosine and MeSH with tyrosinase, and the existence of 2- and 5-methylthio-DOPAs was also suggested. Furthermore, acetone powders from fruits and vegetables oxidized the above diphenolic compounds to bind MeSH.  相似文献   

Neuronal ceroid-lipofuscinosis in longhaired Chihuahuas: clinical, pathologic, and MRI findings     

Nakamoto Y  Yamato O  Uchida K  Nibe K  Tamura S  Ozawa T  Ueoka N  Nukaya A  Yabuki A  Nakaichi M 《Journal of the American Animal Hospital Association》2011,47(4):e64-e70

Neuronal ceroid-lipofuscinosis (NCL) is a rare group of inherited neurodegenerative lysosomal storage diseases characterized histopathologically by the abnormal accumulation of ceroid- or lipofuscin-like lipopigments in neurons and other cells throughout the body. The present article describes the clinical, pathologic, and magnetic resonance imaging (MRI) findings of the NCL in three longhaired Chihuahuas between 16 mo and 24 mo of age. Clinical signs, including visual defects and behavioral abnormalities, started between 16 mo and 18 mo of age. Cranial MRI findings in all the dogs were characterized by diffuse severe dilation of the cerebral sulci, dilated fissures of diencephalons, midbrain, and cerebellum, and lateral ventricular enlargement, suggesting atrophy of the forebrain. As the most unusual feature, diffuse meningeal thickening was observed over the entire cerebrum, which was strongly enhanced on contrast T1-weighted images. The dogs' conditions progressed until they each died subsequent to continued neurologic deterioration between 23 mo and 24 mo of age. Histopathologically, there was severe to moderate neuronal cell loss with diffuse astrogliosis throughout the brain. The remaining neuronal cells showed intracytoplasmic accumulation of pale to slightly yellow lipopigments mimicking ceroid or lipofuscin. The thickened meninges consisted of the proliferation of connective tissues with abundant collagen fibers and mild infiltration of inflammatory cells suggesting neuroimmune hyperactivity. Although the etiology of this neuroimmune hyperactivity is not currently known, MRI findings such as meningeal thickening may be a useful diagnostic marker of this variant form of canine NCL.  相似文献   

Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs     

Nakai M  Kashiwazaki N  Ito J  Maedomari N  Ozawa M  Shino M  Noguchi J  Kaneko H  Kikuchi K 《The Journal of reproduction and development》2011,57(2):183-187

In intracytoplasmic sperm injection (ICSI) technique, a sperm was injected into ooplasm directly using a glass pipette. The fertilization physiology in ICSI is considered quite different from that of the natural fertilization. The different mechanisms for fertilization may be the causes of various results in ICSI. In this paper, we focus on the state of sperm membranes, nuclear or DNA integrity during ICSI procedure and discuss the influence of these factors on fertilization and embryonic development. We also introduce some examples in application of ICSI for new technologies in pigs.  相似文献   

Detection of aac(6’)-Ib-cr in Avian Pathogenic Escherichia coli Isolates in Japan     

Michiko KAWANISHI  Manao OZAWA  Mototaka HIKI  Hitoshi ABO  Akemi KOJIMA  Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1539-1542

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