Evaluation of dietary protein requirement for the growth performance of minor carp,Cirrhinus reba (Hamilton, 1822) fingerlings     

Ajay Kumar Yadav  Sagar Chandra Mandal  Arun Bhai Patel  Pradip Kumar Maurya 《Aquaculture Research》2019,50(11):3343-3349

An experiment was designed to evaluate the requirement of dietary protein for Reba carp, Cirrhinus reba fingerlings using semi‐purified diet. Five isocaloric diets were prepared containing 20%, 25%, 30%, 35% and 40% level of protein using casein, gelatin as a major protein source. The experiment was conducted for 105 days in triplicates to evaluate the growth, survival, feed utilization, carcass composition of C. reba. Mean weight gain was highest (p < .05) in T3. Specific growth rate was highest in T3 and lowest in T1. Similarly, significantly (p < .05) highest net fish yield were recorded in T3. No significant difference was recorded in survivability. Feed conversion ratio, protein efficiency ratio, feed conversion efficiency and protein conversion efficiency were significantly (p < .05) higher for T3 compared with other treatments. Highest carcass protein and lipid values were recorded in T3. So, conclusion can be drawn that 30% crude protein is optimum for overall performance. Furthermore, broken‐line analysis for specific growth rate, final biomass and net fish yield indicated that dietary protein level of 28.148 to 28.253% is optimum for the best growth performance of fingerling C. reba.  相似文献   

Inhibitory effect of <Emphasis Type="Italic">Pistia</Emphasis> tannin on digestive enzymes of Indian major carps: an in vitro study     

Sudipta Mandal  Koushik Ghosh 《Fish physiology and biochemistry》2010,36(4):1171-1180

Aquatic weeds are one of the major unconventional feed ingredients tested for aquafeed formulation. Tannin content in the water lettuce, Pistia, has been quantified (26.67 mg g⁻¹; dry weight) and graded levels of which (12.5–200 μg) have been incorporated in the reaction mixtures to evaluate any change in the in vitro activity of the principal digestive enzymes from the three Indian major carps (IMC), namely rohu (Labeo rohita), catla (Catla catla) and mrigala (Cirrhinus mrigala). Result of the experiment revealed that the Pistia tannin (PT) significantly inhibit/lower the activities of the digestive enzymes from three IMCs in a dose-dependant manner, even at very low concentration. Significant variation in the reduction of the enzyme activities was noticed between the three fish species, as well as between the three enzymes studied. Among the three species studied, digestive enzymes from L. rohita were found to be the most sensitive to the PT, whereas enzymes from C. catla were found to be comparatively least affected. On the other hand, protease and lipase activities were comparatively more affected than the amylase activity. The results of the study suggest that more stress should be given on the elimination of tannin while incorporating feed ingredients of plant origin in fish diets.  相似文献   

Insights into the venom protein components of the egg parasitoid Anastatus japonicus (Hymenoptera: Eupelmidae)     

Chengxing Wang  Fengliang Jin  Surajit De Mandal  Lu Zeng  Yuxin Zhang  Yanyan Hua  Yingying Hong  Can Zhao  Junzhai Li  Dunsong Li  Xiaoxia Xu 《Pest management science》2020,76(6):2113-2126

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Comparative analysis of genome of Edwardsiella tarda by BOX-PCR and PCR-ribotyping     

N.K. Maiti  Arunika Mandal  S. Mohanty  M. Samanta 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):60-63

The Genus Edwardsiella comprises of bacteria differing strongly in their biochemical characteristics, physiological features, natural habitat and pathogenic properties. The most common species of the genus is Edwardsiella tarda, recovered from a variety of environmental and animal sources. In this study 51 isolates including one reference strain obtained from freshwater culture systems were analyzed for their genomic diversity by BOX-PCR and PCR-ribotyping. By comparison of fingerprint of different isolates 27 and 32 genotypes were obtained by PCR-ribotyping and BOX-PCR respectively. Cluster analysis of genetic profile obtained by BOX-PCR and ribotyping clearly showed 9 and 8 clusters respectively. Some correlation between BOX-PCR and ribotyping was observed. Several clusters delineated on the basis of source of isolation in the dendrogram by BOX-PCR with 70% cut off value had corresponding clusters in the ribotyping with 50% cut off value. Some of the genotypes were found to be habitat specific. However, there was mixing and dispersal of most of the genotypes obtained from water, sediment and fish samples. Of both the techniques, BOX-PCR was found to be more discriminating than ribotyping.  相似文献   

Neuropharmacological effects of deltamethrin in rats     

S. Manna  D. Bhattacharyya  T. K. Mandal  S. Dey 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(2):133-136

This study examined the effect of deltamethrin on some of the neuropharmacological paradigms in a rat brain such as the motor co-ordination test using a rotarod, the pentobarbitone-induced sleeping time and pentylenetetrazole (PTZ)-induced convulsion as well as the gamma aminobutyric acid (GABA) level. Albino Wistar rats were used as the experimental animals. Different neuropharmacological paradigms such as the motor co-ordination by the rotarod, pentobarbitone-induced sleeping time and the PTZ-induced convulsion were examined after administering deltamethrin orally at two doses, 150 mg/kg (LD₅₀) and 15 mg/kg (1/10 LD₅₀). The GABA level in the rat brain was estimated by HPLC after a single oral dose of 150mg/kg deltamethrin. Deltamethrin significantly reduced the motor coordination, decreased the onset time and increased the sleeping time duration induced by pentobarbitone. In addition, it also decreased the onset time and increased the duration of convulsions induced by PTZ at 150 mg/kg (LD₅₀) and 15 mg/kg (1/10 LD₅₀), respectively. Further deltamethrin administration decreased the GABA levels in the cerebellum as well as in the whole brain (except the cerebellum) significantly at the LD₅₀ dose level. There was some correlation between the effect of deltamethrin on the central GABA levels and its neuropharmacological effects.  相似文献   

Benign mesenchymal tumour in a goat.     

P C Mandal  B Singh 《Veterinary pathology》1975,12(1):55-56

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Identification of Apoptotic Bodies in Equine Semen     

AB Caselles  A Miro‐Moran  A Morillo Rodriguez  JM Gallardo Bolaños  C Ortega‐Ferrusola  GM Salido  FJ Peña  JA Tapia  IM Aparicio 《Reproduction in domestic animals》2014,49(2):254-262

Apoptosis in the testis is required to ensure an efficient spermatogenesis. However, sometimes, defective germ cells that are marked for elimination during this process escape elimination in the testes, giving rise to ejaculates with increased percentages of abnormal and apoptotic spermatozoa and a high percentage of apoptotic bodies. Apoptosis markers in the ejaculate have been associated with low fertility, either in animals or humans. Therefore, the goal of this study was to investigate whether fresh equine semen contains apoptotic bodies [initially named Merocyanine 540 (M540) bodies] and to study the relationship between the quantity of these bodies and cell concentration, the volume of ejaculate, viability and motility. Moreover, we also studied whether the presence apoptotic bodies in fresh semen was related to the resistance of the stallion spermatozoa to being incubated at 37°C or being frozen and thawed. Fresh equine semen was stained with fluorescent dyes such as M540 and Annexin‐V. Active Caspase 3 was studied in fresh semen through Western blotting and immunofluorescence with a specific antibody. Sperm kinematics was assessed in fresh, incubated and thawed samples using computer‐assisted semen analysis, and viability was evaluated with the LIVE/DEAD Sperm Viability Kit. Overall, our results demonstrate for the first time the presence of apoptotic bodies in equine semen. The quantity of apoptotic bodies was highly variable among stallions and was positively correlated with Caspase 3 activity in fresh samples and negatively correlated with the viability and motility of stallion spermatozoa after the cryopreservation process.  相似文献   

Systemic Immunosuppression by Methylprednisolone and Pregnancy Rates in Goats Undergoing the Transfer of Cloned Embryos     

C Feltrin  CA Cooper  N Mohamad‐Fauzi  VHV Rodrigues  LH Aguiar  S Gaudencio‐Neto  LT Martins  CEM Calderón  AS Morais  IS Carneiro  TM Almeida  ING Silva  JL Rodrigues  EA Maga  JD Murray  AB Libório  LR Bertolini  M Bertolini 《Reproduction in domestic animals》2014,49(4):648-656

The presence of the zona pellucida has been perceived as a requirement for the oviductal transfer of cloned embryos at early stages of development while protecting the embryo from an immune system response. We hypothesized that steroid hormone therapy could reduce a potential cellular immune response after the transfer of zona‐free cloned embryos into the oviduct of recipient female goats. In Experiment 1, seven does were used to study the systemic immunosuppressant effect of the methylprednisolone administration (for 3 days) on blood cell counts. Whole blood was collected prior to treatment with methyprednisolone and then on Days 1, 2, 3, 4, 7, 14, 21 and 28 after the first dose of methylprednisolone for the analysis of haematological parameters. Methylprednisolone treatment significantly reduced circulating white blood cells and neutrophils in comparison with pre‐treatment levels, demonstrating a systemic immunosuppressant effect. In Experiment 2, a group of 58 does were used as recipient females to study the effect of administration of methylprednisolone for 3 days on the establishment of pregnancies after the transfer of zona‐free cloned embryos into the oviducts. No effects on pregnancy rates on Day 30 were observed regarding the distinct treatment groups (control vs. methylprednisolone), the source of oocytes (in vivo‐ vs in vitro‐matured) or the presence or absence of the zona pellucida in embryos. In summary, methylprednisolone was effective at inducing a systemic immunosuppressed state in goats, but the treatment prior to embryo transfer did not affect pregnancy rates. Moreover, pregnancy rates were similar between zona‐free and zona‐intact goat cloned embryos.  相似文献   

Control of transcription     

Mandal RK 《Science (New York, N.Y.)》1973,182(4117):1165-1169

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Imperfect institutional innovation for irrigation management in Bangladesh     

M. A. S. Mandal 《Irrigation and Drainage Systems》1987,1(3):239-258

This paper discusses the nature and consequences of institutional innovation being pursued in recent years for the organization and management of minor irrigation in Bangladesh. The comparative analysis presented in this paper suggests that there are no significant variations of performance between the mainstream management institutions, except that tubewells under BRDB-Cooperative groups performed less well than others, nor are there any significant associations between major technical factors and irrigation performance (other than nominal pump capacity). What appears to be more important is that the economic institutions such as payment system for water is strongly associated with productivity and equity performance; sharecropping with water, which has emerged, or been popularized, with the privatization programme, has negative impact on productivity and skews benefits of irrigation towards pump owners and managers. It is concluded that so far imperfectly designed institutions, for example privatization of equipment, appear to have failed to give rise to greater technical and allocative efficiency or equity.Abbreviations BADC Bangladesh Agricultural Development Corporation - BRDB Bangladesh Rural Development Board - BKB Bangladesh Krishi (Agricultural) Bank - GB Grameen (Rural) Bank - CARE Cooperative for American Relief Everywhere - KSS Farmers' Cooperative Society - IMP Irrigation Management Programme  相似文献