Complications and outcomes of thoracoscopic-assisted lung lobectomy in dogs     

Jacqueline E. Scott BVSc  DVSc  DACVS-SA  Danielle A. Auzenne BVSc  DVM  Federico Massari DVM  DECVS  Ameet Singh DVM  DVSc  DACVS-SA  Victoria Donovan MSc  Philipp D. Mayhew BVM&S  DACVS-SA  Brad Case DVM  MS  DACVS-SA  Valery F. Scharf DVM  MS  DACVS-SA  Nicole Buote DVM  DACVS-SA  Mandy L. Wallace DVM  MS  DACVS-SA 《Veterinary surgery : VS》2023,52(1):106-115

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Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers     

Gernot Schmoock  Mandy Elschner  Lisa D Sprague 《Acta veterinaria Scandinavica》2015,57(1)

Background

A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.

Findings

Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively.

Conclusions

This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis.

Electronic supplementary material

The online version of this article (doi:10.1186/s13028-015-0104-4) contains supplementary material, which is available to authorized users.  相似文献   

Coping with information gaps in stock productivity for rebuilding and achieving maximum sustainable yield for grouper–snapper fisheries     

William J Harford  Skyler R Sagarese  Mandy Karnauskas 《Fish and Fisheries》2019,20(2):303-321

Maintaining fish stocks at optimal levels is a goal of fisheries management worldwide; yet, this goal remains somewhat elusive, even in countries with well‐established fishery data collection, assessment and management systems. Achieving this goal often requires knowledge of stock productivity, which can be challenging to obtain due to both data limitations and the complexities of marine populations. Thus, scientific information can lag behind fishery policy expectations in this regard. Steepness of the stock–recruitment relationship affects delineation of target biomass level reference points, a problem which is often circumvented by using a proxy fishing mortality rate (F) in place of the rate associated with maximum sustainable yield (F_MSY). Because MSY is achieved in the long term only if an F proxy is happenstance with F_MSY, characterizing productivity information probabilistically can support reference point delineation. For demersal stocks of equatorial and tropical regions, we demonstrate how the use of a prior probability distribution for steepness can help identify suitable F proxies. F proxies that reduce spawning biomass per recruit to a target percentage of the unfished quantity (i.e., SPR) of 40% to 50% SPR had the highest probabilities of achieving long‐term MSY. Rebuilding was addressed through closed‐loop simulation of broken‐stick harvest control rules. Similar biomass recovery times were demonstrated for these rules in comparison with more information‐intensive rebuilding plans. Our approach stresses science‐led advancement of policy through a lens of information limitations, which can make the assumptions behind rebuilding plans more transparent and align management expectations with biological outcomes.  相似文献   

Lower urinary tract transitional cell carcinoma in cats: Clinical findings,treatments, and outcomes in 118 cases     

Maureen A. Griffin  William T. N. Culp  Michelle A. Giuffrida  Peter Ellis  Joanne Tuohy  James A. Perry  Allison Gedney  Cassie N. Lux  Milan Milovancev  Mandy L. Wallace  Jonathan Hash  Kyle Mathews  Julius M. Liptak  Laura E. Selmic  Ameet Singh  Carrie A. Palm  Ingrid M. Balsa  Philipp D. Mayhew  Michele A. Steffey  Robert B. Rebhun  Jenna H. Burton  Michael S. Kent 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(1):274-282

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Identification of a second Asian soybean rust resistance gene in Hyuuga soybean     

Kendrick MD  Harris DK  Ha BK  Hyten DL  Cregan PB  Frederick RD  Boerma HR  Pedley KF 《Phytopathology》2011,101(5):535-543

ABSTRACT Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. Three isolates elicited different responses from soybean accessions PI 462312 (Ankur) (Rpp3) and PI 506764 (Hyuuga) (Rpp?[Hyuuga]). In all, 11 of the new accessions yielded responses identical to either PI 462312 or Hyuuga and 1 of the new accessions, PI 417089B (Kuro daizu), differed from all others. Additional screening of Hyuuga-derived recombinant inbred lines indicated that Hyuuga carries two resistance genes, one at the Rpp3 locus on Gm06 and a second, unlinked ASR resistance gene mapping to Gm03 (LG-N) near Rpp5. These findings reveal a natural case of gene pyramiding for ASR resistance in Hyuuga and underscore the importance of utilizing multiple isolates of P. pachyrhizi when screening for ASR resistance.  相似文献   

Modulation of gene expression via disruption of NF-kappaB signaling by a bacterial small molecule     

Kravchenko VV  Kaufmann GF  Mathison JC  Scott DA  Katz AZ  Grauer DC  Lehmann M  Meijler MM  Janda KD  Ulevitch RJ 《Science (New York, N.Y.)》2008,321(5886):259-263

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A highly conserved neutralizing epitope on group 2 influenza A viruses   总被引：1，自引：0，他引：1  

Ekiert DC  Friesen RH  Bhabha G  Kwaks T  Jongeneelen M  Yu W  Ophorst C  Cox F  Korse HJ  Brandenburg B  Vogels R  Brakenhoff JP  Kompier R  Koldijk MH  Cornelissen LA  Poon LL  Peiris M  Koudstaal W  Wilson IA  Goudsmit J 《Science (New York, N.Y.)》2011,333(6044):843-850

Current flu vaccines provide only limited coverage against seasonal strains of influenza viruses. The identification of V(H)1-69 antibodies that broadly neutralize almost all influenza A group 1 viruses constituted a breakthrough in the influenza field. Here, we report the isolation and characterization of a human monoclonal antibody CR8020 with broad neutralizing activity against most group 2 viruses, including H3N2 and H7N7, which cause severe human infection. The crystal structure of Fab CR8020 with the 1968 pandemic H3 hemagglutinin (HA) reveals a highly conserved epitope in the HA stalk distinct from the epitope recognized by the V(H)1-69 group 1 antibodies. Thus, a cocktail of two antibodies may be sufficient to neutralize most influenza A subtypes and, hence, enable development of a universal flu vaccine and broad-spectrum antibody therapies.  相似文献   

Applications of pedigree-based genome mapping in wheat and barley breeding programs   总被引：1，自引：0，他引：1  

Mandy Christopher  Emma Mace  David Jordan  David Rodgers  Paul McGowan  Ian Delacy  Phillip Banks  John Sheppard  David Butler  David Poulsen 《Euphytica》2007,154(3):307-316

The aim of the pedigree-based genome mapping project is to investigate and develop systems for implementing marker assisted selection to improve the efficiency of selection and increase the rate of genetic gain in breeding programs. Pedigree-based whole genome marker application provides a vehicle for incorporating marker technologies into applied breeding programs by bridging the gap between marker–trait association and marker implementation. We report on the development of protocols for implementation of pedigree-based whole genome marker analysis in breeding programs within the Australian northern winter cereals region. Examples of applications from the Queensland DPI&F wheat and barley breeding programs are provided, commenting on the use of microsatellites and other types of molecular markers for routine genomic analysis, the integration of genotypic, phenotypic and pedigree information for targeted wheat and barley lines, the genomic impacts of strong selection pressure in case study pedigrees, and directions for future pedigree-based marker development and analysis.  相似文献   

Participation and model-building: Lessons learned from the bukittinggi workshop     

Jerome K. Vanclay  Mandy Haggith  Carol J. Pierce Colfer 《Small-Scale Forestry》2003,2(2):135-154

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Unlocking complexity: The importance of idealisation in simulation modelling     

Mandy Haggith  Ravi Prabhu 《Small-Scale Forestry》2003,2(2):293-312

Idealisation is the process of finding simple representations of the real-world whilst conceptualising a model. There are three ways to limit complication in a model of a complex real-world: byfocussing the scope of the modelling process onto a clearly defined issue; byidealising elements of the real-world during model conceptualisation; and bysimplifying the implemented simulation program. Careful idealisation has the greatest potential for increasing model tractability whilst generating insights during the model design process. The Forest Land Oriented Resource Envisioning System (FLORES) project deals with social forest landscapes which are highly complex. Benefits of idealisation are demonstrated using six examples from this modelling work. These examples encompass issues dealing with land tenure, forest management, economic values, social diversity, communication and collaboration. Each example illustrates a different method to achieve an idealisation which yields insights relevant for policy players. A number of lessons about idealisation are also identified: (1) sometimes it is only possible to recognise what is key by omitting it; (2) an effective idealisation is not just achieved by leaving things out, or adding them back in; it can also be achieved by restructuring the representation; (3) it is important challenge the use of different units where consistency is possible; (4) it is easier to keep a simple model simple, than to make simple modifications to a large model. Similarly, it is easier to generate insights with a simple concept for a sub-model than with a simple modification to an existing model; and (5) even the most useful idealisations may have a limited shelf-life. Thanks to Bill Ritchie, Carol Colfer, Bruce Campbell, Herry Purnomo, Wavell Standa-Gunda, Richard Dudley and Jerry Vanclay for helpful comments on earlier drafts of this paper, and to all the other Florists for engaging in debate about this modelling experience. We are grateful to the UK’s Department for International Development (DFID) and the European Community for financial support of this project.  相似文献