首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   309篇
  免费   19篇
林业   34篇
农学   9篇
  63篇
综合类   58篇
农作物   13篇
水产渔业   23篇
畜牧兽医   86篇
园艺   9篇
植物保护   33篇
  2023年   1篇
  2022年   9篇
  2021年   13篇
  2020年   22篇
  2019年   12篇
  2018年   7篇
  2017年   19篇
  2016年   8篇
  2015年   13篇
  2014年   17篇
  2013年   13篇
  2012年   31篇
  2011年   36篇
  2010年   23篇
  2009年   7篇
  2008年   24篇
  2007年   15篇
  2006年   11篇
  2005年   10篇
  2004年   8篇
  2003年   11篇
  2002年   4篇
  2001年   1篇
  2000年   4篇
  1991年   2篇
  1984年   1篇
  1983年   1篇
  1975年   1篇
  1973年   1篇
  1959年   1篇
  1952年   1篇
  1950年   1篇
排序方式: 共有328条查询结果,搜索用时 15 毫秒
101.
102.
A 6-week-old female Simmental calf was evaluated for acute non-ambulatory tetraparesis. Physical and laboratory examinations revealed no clinically relevant abnormalities. Neurological findings were consistent with acute, progressive and painful cervical myelopathy. Radiographs displayed a fractured odontoid process (dens axis) and vertebral step misalignment at the fracture site. A traumatic origin was suspected. Advanced diagnostic imaging was considered to allow better planning of potential surgical stabilisation and to exclude any additional lesions of the cervical vertebral column. However, during trailer transportation to the advanced diagnostic imaging and surgery site, the calf deteriorated neurologically and was humanely euthanised. Magnetic resonance imaging (MRI) and computed tomography (CT) were performed immediately post-mortem for scientific reasons. The MRI examination reflected the radiographic findings and confirmed severe spinal cord compression at the fracture site. In addition, a T2W-hyperintense signal change within the paravertebral soft tissue dorsal to the fracture site was indicative of a traumatic event. CT identified the fracture site at the synchondrosis between the odontoid process and the body of the axis, and this finding was confirmed by post-mortem examination. Advanced diagnostic imaging and post-mortem examination did not identify any other cervical lesion. In summary, this calf was diagnosed with a traumatic odontoid process synchondrosis fracture, which has not been reported previously in calves but presents a challenging and well-known fracture type in young children. This case report indicates that the odontoid process synchondrosis is a potential predisposed injury site and that traumatic odontoid process synchondrosis fractures should be considered as a potential differential in calves with acute cervical pain and/or signs of a cervical myelopathy.  相似文献   
103.
104.
Patients suffering from coeliac disease have to avoid traditional cereals-based products and depend on the availability of gluten-free alternatives. The gluten-free bread matrix and its foam stability are strongly affected by the choice of ingredients. In this study, the impact of quinoa white flour on bread quality parameters, in particular volume, has been investigated. The pseudocereal proved to be a suitable substrate for dough aeration using yeast, since considerably more glucose and a higher activity of α-glucosidase were found in comparison to rice and corn flour. Consequently, quinoa white flour was used to replace 40–100% of the rice and corn flour in a gluten-free control recipe. As a result, quinoa white flour enhanced the specific volume by 33%, which was related to the absence of bran components and the increased α-glucosidase activity. The significance of the latter was proven by separately adding sucrose and fungal amyloglucosidase to the control recipe. Moreover, the crumb featured homogeneous and finely distributed gas bubbles and the taste was not compromised. Thus, it was possible to improve the quality of gluten-free bread by using quinoa white flour, which might be a relief for coeliac patients.  相似文献   
105.
The speciose Crustacea is the largest subphylum of arthropods on the planet after the Insecta. To date, however, the only publically available sequenced crustacean genome is that of the water flea, Daphnia pulex, a member of the Branchiopoda. While Daphnia is a well-established ecotoxicological model, previous study showed that one-third of genes contained in its genome are lineage-specific and could not be identified in any other metazoan genomes. To better understand the genomic evolution of crustaceans and arthropods, we have sequenced the genome of a novel shrimp model, Neocaridina denticulata, and tested its experimental malleability. A library of 170-bp nominal fragment size was constructed from DNA of a starved single adult and sequenced using the Illumina HiSeq2000 platform. Core eukaryotic genes, the mitochondrial genome, developmental patterning genes (such as Hox) and microRNA processing pathway genes are all present in this animal, suggesting it has not undergone massive genomic loss. Comparison with the published genome of Daphnia pulex has allowed us to reveal 3750 genes that are indeed specific to the lineage containing malacostracans and branchiopods, rather than Daphnia-specific (E-value: 10−6). We also show the experimental tractability of N. denticulata, which, together with the genomic resources presented here, make it an ideal model for a wide range of further aquacultural, developmental, ecotoxicological, food safety, genetic, hormonal, physiological and reproductive research, allowing better understanding of the evolution of crustaceans and other arthropods.  相似文献   
106.
Neosaxitoxin (NeoSTX) is a specific reversible blocker of voltage gated sodium channels on excitable cells. In the last decade, it has been tested in a number of interesting clinical trials, however there is still little information available on mammalian toxicity. Rats were treated for 12 weeks with doses of 1, 3 or 6 μg/kg of subcutaneous NeoSTX. At weeks 12 and 17, animals were sacrificed and blood samples collected for hematological and biochemical analysis. Organs were harvested for weight determination and histopathological assessments. The lowest acute toxicity via the intraperitoneal (ip) route was (30.35 μg/kg) and there was no significant difference between intramuscular and subcutaneous routes (11.4 and 12.41 μg/kg). The NeoSTX adiministration did not produce lethality at week 12 and after five weeks of suspension. NeoSTX 6 μg/kg ip produced reductions (p < 0.05) in body weight and food intake, and increased blood level of total and direct bilirubin, GGT and SGOT at week 12; all of these were reversed in the recovery period. NeoSTX 1 and 3 μg/kg ip did not show significant changes with the control group. Histopathological presentations were normal in all groups. This study revealed that NeoSTX is safe in vivo, giving a reliable security margin for its use like a local anesthetic.  相似文献   
107.
The objectives of this study were to examine associations between perceived psychosocial stress and farm injury among men and women in Ontario, Canada. Cross-sectional data from the Ontario Farm Family Health Study were used to investigate perceived levels of stress, farm injuries and their interrelationships. Age-standardized rates of injury were 13.3/100/year and 3.8/100/year for men and women, respectively. The most common types of injury were strains/sprains/torn ligaments and cuts/lacerations. Approximately 18% of men and 11% of women reported that their lives were "very stressful." Common sources of stress were money worries and feeling overworked. The risk for farm injury increased with level of stress. For men, the adjusted odds ratios for injury were: 1.00 (referent), 1.02 (95% CI: 0.72, 1.42), and 1.61 (95% CI: 1.08, 2.41)for lowest to highest stress levels, respectively. For women, adjusted odds ratios were: 1.00 (referent), 1.43 (95% CI: 0.83, 2.47), and 2.73 (95% CI: 1.38, 5.39). These risks were especially pronounced among women who were not employed off the farm. This study represents a novel quantitative analysis examining associations between perceived psychological stress and farm injury. Future research should investigate these associations in other farm populations, confirm their temporal directions, and further explore the effect of gender on the strength of these associations.  相似文献   
108.
Journal of Plant Diseases and Protection - European ash dieback caused by the alien, invasive ascomycete species Hymenoscyphus fraxineus currently represents, along with its side effects, the...  相似文献   
109.
The water balance in the soil profile and soil moisture dynamic (available water content [%]) under sugar beet 2009 and spring barley 2010 were investigated in selected treatments of a long-term K-fertilization trial on a chernozem developed from loess in Bernburg, Germany (21% clay, 73% silt, 1.5% Corg), which received an annual K-fertilization of 0/0 and 249/124.5 kg ha?1, respectively, to sugar beet/spring barley within the last 14 years. Intensive K-fertilization increased the K content of topsoil and subsoil as well as available water capacity up to 60 cm soil depth. Under water stress conditions in August 2009, the treatment with high K-fertilization achieved the highest sugar beet yield, sugar content (°S) and white-sugar-yield at final harvest. Combined with the higher beet yield, the water use efficiency was also increased and the water pool of the subsoil was used more efficiently. In the following year (2010), weather conditions were relatively wet. Under these conditions, no differences in yield parameters and water consumption of sugar beet and spring barley between both K-levels were observed.  相似文献   
110.
Micronutrients when fed around peripartum may reduce the stress induced by cortisol. With this objective, 24 Sahiwal (SW) and 24 Karan Fries (KF) cows and 24 Murrah (Mu) buffaloes were taken and divided into four groups of six each. Vitamin E (VE), zinc (Zn) and copper were supplemented from 30 days pre- to 30 days postcalving in groups 1, 2 and 3. Animals without supplementation served as control. Blood sampling was done on days 30, 15, 7 and 3 precalving; at calving; and on days 3, 7, 15 and 30 postcalving. Plasma cortisol levels were measured by ELISA. Supplementation of VE and Zn significantly (P?<?0.05) reduced plasma cortisol levels at calving and at 30 days postcalving. KF cows exhibited best results with VE, whereas SW cows and Mu buffaloes responded best to Zn. Copper increased the peripartum cortisol levels. Reduction in cortisol levels may help in keeping the animal healthy and stronger to fight immuno-suppression generally observed around the period of peripartum.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号