The future of veterinary medicine     

Wolthers K  de Rooij D 《Tijdschrift voor diergeneeskunde》2006,131(14-15):548-549

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Exclusion of classical swine fever by PCR test     

de Groot H 《Tijdschrift voor diergeneeskunde》2006,131(21):790

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Bluetongue in The Netherlands; description of the first clinical cases and differential diagnosis. Common symptoms just a little different and in too many herds     

van Wuijckhuise L  Dercksen D  Muskens J  de Bruijn J  Scheepers M  Vrouenraets R 《Tijdschrift voor diergeneeskunde》2006,131(18):649-654

For the first time Bluetongue (BT) has been diagnosed in the Netherlands. The clinical symptoms of BT on five farms during the first outbreak ever in the Netherlands are described. Fever and swollen sensitive coronets leading to reluctance to stand and walk were sometimes the first symptoms. Later lesions in the mouth occurred with foamy salivation and respiratory problems. In other cases a swollen head with swollen lips and foamy salivation were the first clinical signs. Also sudden death occurred. In the first sixteen confirmed cases morbidity and mortality were lower than described in outbreaks in other countries. Good collaboration between practitioners, specialists of the Animal Health Service (GD-Deventer), and specialists of the Food and Consumer Product Safety Authority (VWA) and CIDC-Lelystad (Wageningen UR) led to a rapid notification and ultimately confirmation of the suspected diagnosis BT.  相似文献   

Kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection     

Oliveira HM  Segundo MA  Lima JL  Grassi V  Zagatto EA 《Journal of agricultural and food chemistry》2006,54(12):4136-4140

A sequential injection system for the automatic determination of glycerol in wine and beer was developed. The method is based on the rate of formation of NADH from the reaction of glycerol and NAD+ catalyzed by the enzyme glycerol dehydrogenase in solution. The determination of glycerol was performed between 0.3 and 3.0 mmol L(-1) (0.028 and 0.276 g L(-1)), and good repeatability was attained (rsd < 3.6%, n = 5) for all samples tested. The determination rate was 54 h(-1), the reagent consumption was only 0.75 micromol of NAD+ and 5.4 ng of enzyme per assay, and the waste production was 2.12 mL per assay. Results obtained for samples were in agreement with those obtained with the batch enzymatic method.  相似文献   

Changes in the free amino acid contents of honeys during storage at ambient temperature     

Iglesias MT  Martín-Alvarez PJ  Polo MC  de Lorenzo C  Gonzalez M  Pueyo E 《Journal of agricultural and food chemistry》2006,54(24):9099-9104

This study was carried out to establish the changes in the free amino acid contents of floral honeys, honeydew honeys, and blend honeys during storage at room temperature and to test the capacity of the amino acids to distinguish the origin of the honeys after storage. For this purpose, 54 artisanal honeys (39 floral, 5 honeydew, and 10 blend) were studied. Samples were taken from recently collected honeys and at 3, 6, 9, 12, 16, 20, and 24 months after harvesting. The contents of most of the free amino acids were found to decrease with storage time, with the greatest reduction observed in the first 9 months. The contents of the amino acids aspartic acid, beta-alanine, and proline increased in the first few months after storage, reaching maximum values at 6 months, suggesting the possible existence of enzymatic activities. The application of stepwise discriminant analysis to the free amino acid content data demonstrated that the contents of the amino acids valine, beta-alanine, gamma-aminobutyric acid, serine, isoleucine, alpha-alanine, ornithine, and glutamine correctly assigned 87% of honeys to their group of origin: floral, honeydew, or blend.  相似文献   

Prolonged elevated atmospheric CO₂ does not affect decomposition of plant material     

Marie-Anne de Graaff  Johan Six  Chris van Kessel 《Soil biology & biochemistry》2006,38(1):187-190

Prolonged elevated atmospheric CO₂ might alter decomposition. In a 90-day incubation study, we determined the long-term (9 years) impact of elevated CO₂ on N mineralization of Lolium perenne and Trifolium repens plant material grown at ambient and elevated CO₂ and low- and high-¹⁵N fertilizer additions. No significant differences were observed in recovery rates between any of the treatments, except an N addition effect was observed for L. perenne (0.4 versus 0.5% day⁻¹ in high versus low N). The results suggest that elevated CO₂ did not change plant N mineralization in any of the soils, because of a surplus of available N in the fertilized and leguminous systems, and because of insignificant plant responses to elevated CO₂ in the low soil N availability systems.  相似文献   

Quality control in veterinary medicine     

de Bruijckere G 《Tijdschrift voor diergeneeskunde》2006,131(8):300; author reply 300

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Star clinics AUV     

Croughs ER  van der Drift JW  Gelders GM  van de Kerkhof WA  Paping AC  Smeets LG  Verhey EH  Wetzels HJ 《Tijdschrift voor diergeneeskunde》2006,131(9):345

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Mycobacterium bovis infection in a dog     

Ellis MD  Davies S  McCandlish IA  Monies R  Jahans K  de la Rua-Domenech R 《The Veterinary record》2006,159(2):46-48

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Morphologic and functional features of the canine cruciate ligaments     

de Rooster H  de Bruin T  van Bree H 《Veterinary surgery : VS》2006,35(8):769-780

OBJECTIVE: To review the gross, microscopic, and functional anatomy of the cranial cruciate ligament (CCL) in dogs. STUDY DESIGN: Literature review. METHODS: Reports of the anatomy and function of the cruciate ligaments in dogs were retrieved by search of the 1975-2005 PubMed database. RESULTS: The CCL has an important biomechanical function resisting cranial drawer, hyperextension, and internal rotation and acts to fine tune and guide the stifle through its rolling and sliding motion. It has a complex architecture, and distinct geographic regions within the ligament have different functional roles depending on the angle and loading conditions. Collagen type I is the main component of the extracellular matrix; the fibrils have a crimped structure. The cruciate ligaments are almost completely covered by synovium, protecting them from synovial fluid. Cruciate blood supply is mainly of soft tissue origin. The intraligamentous network is relatively limited whereas the core of the middle third of the CCL is even less well vascularized. Neurohistologic studies are very limited in the dog. Various mechanoreceptors and proprioceptive receptors have been identified within the substance of the cruciate ligaments. CONCLUSIONS: CCL structural characteristics play an important part in its complex behaviour with the crimped pattern of the collagen fibrils being an important determinant of its biomechanical properties. In contrast to reports of managing CCL rupture, there are few reports describing the microanatomy and neurovascular morphology of the cruciate ligaments. CLINICAL RELEVANCE: Cruciate disease is likely multi-factorial. Improved understanding of CCL degradation leading to CCL rupture is critical to development of new diagnostic tests for cruciate disease in dogs. Appropriate intervention during the early stages of disease process might preserve CCL structural properties by preventing further collagen degradation. Accurate knowledge of functional and fiber bundle anatomy is imperative for reconstruction and restoration of normal stifle joint physiology. Reconstructive goals should alleviate existing instability and mimic normal kinematics. Knowledge of the exact function of the CCL in the neuromuscular control around the stifle joint could possibly explain osteoarthritis progression after CCL damage.  相似文献