Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens          下载免费PDF全文

PJ Groves  SM Sharpe  WI Muir  A Pavic  JM Cox 《Australian veterinary journal》2016,94(10):387-393

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Negative pressure wound therapy using a portable single‐use device for free skin grafts on the distal extremity in seven dogs          下载免费PDF全文

AJ Miller  RG Cashmore  AM Marchevsky  M Havlicek  PM Brown  SM Fearnside 《Australian veterinary journal》2016,94(9):309-316

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Effects of pimobendan on myocardial perfusion and pulmonary transit time in dogs with myxomatous mitral valve disease: a pilot study          下载免费PDF全文

SM Apple  G Menciotti  L Braz‐Ruivo  S Crosara  J Häggström  M Borgarelli 《Australian veterinary journal》2016,94(9):324-328

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Frequency of shedding of respiratory pathogens in horses recently imported to the United States          下载免费PDF全文

Fauna Leah Smith  Johanna L. Watson  Sharon J. Spier  Isabelle Kilcoyne  Samantha Mapes  Claudia Sonder  Nicola Pusterla 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(4):1436-1441

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Third‐compartment cannulation in alpacas using a polyurethane gastrostomy tube     

GW Smith  MP Gerard  NB Campbell  DM Foster  SM Smith  JL Davis 《Australian veterinary journal》2009,87(12):487-491

Objective To develop a simple and effective surgical technique for third‐compartment cannulation in alpacas. Design Prospective study using six adult male alpacas. Methods General anaesthesia was induced and a polyurethane gastrostomy tube was surgically implanted into the distal portion of the third compartment. Results Three of the alpacas retained their cannulas for a 100‐day period; however, three cannulas were dislodged during the study. Two of the three dislodged cannulas were replaced during a second surgical procedure. Cannulas were well tolerated by the alpacas and all animals remained clinically healthy during the study period. Third compartment contents did not leak from the cannulation site. The tubes were manually removed following the completion of the study and the small defect in the body wall quickly healed over in all animals. Conclusion Surgical placement of polyurethane tubes designed for percutaneous endoscopic gastrostomy is a useful method of cannulating the third compartment in camelids. This technique can be used for experimental studies and possibly could be used for nutritional support and fluid therapy in sick camelids that might need long‐term care.  相似文献   

Evaluation of the humoral immune response and fecal shedding in weanling foals following oral and intra-rectal administration of an avirulent live vaccine of Lawsonia intracellularis     

Pusterla N  Hilton H  Wattanaphansak S  Collier JR  Mapes SM  Stenbom RM  Gebhart C 《Veterinary journal (London, England : 1997)》2009,182(3):458-462

Equine proliferative enteropathy (EPE) caused by Lawsonia intracellularis has recently been recognized as an emerging disease in foals. Whilst the clinical entity, diagnostic evaluation and treatment of affected foals have been well established and described, preventive measures for EPE have remained largely unaddressed. The objectives of this study were to investigate the humoral immune response and onset and duration of fecal shedding in foals after oral and intra-rectal administration of a modified-live vaccine of L. intracellularis. Foals were vaccinated twice, 3 weeks apart, via oral drenching after pre-medication with a proton-pump inhibitor (omeprazole; group 1), intra-rectally (group 2) or orally without any pre-medication (group 3). The health status of the foals was monitored daily, with feces and serum collected at regular intervals for Polymerase Chain Reaction (PCR) and serology. All foals remained healthy and no adverse vaccine reactions were observed. Fecal shedding lasted from 1 to 12 days and was mainly detected in foals receiving the intra-rectal vaccine 11-15 days following the first vaccine administration. Serological responses were measured in the majority of the vaccinated foals. All foals vaccinated intra-rectally seroconverted after the first vaccine, compared to 50% and 0% of foals in groups 1 and 3, respectively. Pre-medication with omeprazole prior to oral vaccination in group 1 foals led to an earlier and stronger detectable humoral response compared to non pre-medicated foals.  相似文献   

Molecular mechanism of Chinese medicine formula (Bushen Bugu Tang) for the treatment of postmenopausal osteoporosis     

TENG Xiao-ying  WANG Lian-tang  Huang L  Kumta SM 《园艺学报》2004,20(11):2005-2010

AIM: To study the molecular mechanism of Chinese medicine formula Bushen Bugu Tang (BSBGT) for the treatment of postmenopausal osteoporosis (PMO). METHODS: Forty female SD rats were divided into four groups: the sham group, OVX group, estrogen treatment group and BSBGT treatment group, ten for each, difference operation and treatment were given. Subsequently, bone mineral density (BMD), bone histomorphometric changes and collagen type I expression in the femur of these rats were examined. In order to position the target of the formula acting on bone, the effect of BSBGT on regulation of mRNA levels of RANKL and OPG, two key factors for regulating osteoclastogenesis, by using rat calvarial-derived stromal cell cultrues, was elucidated. RESULTS: The BSBGT formula was found as effective as estrogen in preventing the reduction of BMD, the trabecular area and trabecular numbers in femora and also the decrease in collagen type I expression following ovariectomy. RT-PCR resulted showed that both estrogen and BSBGT significantly up-regulated OPG mRNA level and down-regulated RANKL mRNA level, resulting in an increased ratio of OPG/RANKL in cultured rat stromal cells. CONCLUSIONS: The ratio of OPG/RANKL within the osseous environment determines whether bone formation predominates bone resorption. We therefore concluded that the BSBGT formula might be as effective as estrogen in prevention of postmenopausal osteoporosis through the mechanism of up-regulating RANKL expression and down-regulating OPG expression in osteoblast/stromal cells.  相似文献   

Use of viral loads in blood and nasopharyngeal secretions for the diagnosis of EHV-1 infection in field cases     

Pusterla N  Mapes S  Wilson WD 《The Veterinary record》2008,162(22):728-729

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Capacitation of Frozen Thawed Buffalo Bull (Bubalus bubalis) Spermatozoa with Higher Heparin Concentrations     

A Mehmood  M Anwar  SM Saqlan Naqvi 《Reproduction in domestic animals》2007,42(4):376-379

The present study was conducted to examine the effect of high heparin concentration on capacitation of buffalo spermatozoa with a short incubation time. Frozen thawed spermatozoa from three buffalo bulls were pooled and treated with either 50, 100 or 200 microg/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and in vitro fertilization rate (per cent cleavage rate, per cent cleavage index). Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with Coomassie G-250 to evaluate the response as compared with control (0 heparin + calcium ionophore). Significantly higher percentage of acrosome reaction (AR) spermatozoa was noted after heparin treatment (36.8-48.2%) as compared with control (8.1% ; p < 0.05) but differences among the three heparin concentrations were non-significant. However, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 microg/ml heparin (80.4 and 75.9% cleavage rate, respectively) as compared with 200 microg/ml heparin (47.2% cleavage rate; p < 0.001). It is concluded that buffalo spermatozoa capacitated with 50-100 microg/ml heparin had significantly higher ability to improve in vitro fertilization rate in buffalo.  相似文献   

Marking to weaning production aspects of lambs provided with NSAID analgesia compared with lambs receiving no analgesia at the time of elastrator ring marking     

AH Small  S Belson  H Brewer  SM Schmoelzl 《Australian veterinary journal》2021,99(1-2):40-43

The provision of analgesia at the time of marking has been adopted by the Australian sheep industry, but data on production benefits are lacking. In the current study, alternate lambs were provided with either meloxicam (non‐steroidal anti‐inflammatory drug [NSAID], n = 781) or no analgesia (NONE, n = 822) at the time of ring castration and tail docking. Six distinct management groups of lambs were studied. Lambs were weighed immediately before marking and then again at weaning. There was no significant effect of treatment on average daily gain between marking and weaning in cross‐bred lambs. In Merino lambs, average daily gain was 5 g/day lower (P < 0.005) in lambs receiving NSAID, but this may not be biologically significant. Lamb losses were significantly (P < 0.05) lower in the NSAID group (1.1%) than in the NONE group (2.7%). This observation is worth validating in larger studies, particularly considering that lamb mortality is a significant cost to production and welfare concern.  相似文献