Detection of Bemisia tabaci Mediterranean cryptic species on soybean in São Paulo and Paraná States (Brazil) and interaction of cowpea mild mottle virus with whiteflies     

Vinicius Henrique Bello  Felipe Barreto da Silva  Luís Fernando Maranho Watanabe  Eduardo Vicentin  Cristiane Muller  Regiane Cristina Oliveira de Freitas Bueno  Jessica Costa Santos  Bruno Rossitto De Marchi  Angélica Maria Nogueira  Valdir Atsushi Yuki  Julio Massaharu Marubayashi  Maria Márcia Pereira Sartori  Marcelo Agenor Pavan  Murad Ghanim  Renate Krause-Sakate 《Plant pathology》2021,70(6):1508-1520

Since the detection of Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) species in Brazil in the early 1990s, this whitefly has been the predominant species and the main viral vector in the country. In this work, whiteflies were collected from commercial soybean fields near and far from greenhouses where Mediterranean (MED) species had previously been detected infesting vegetable crops. Results indicated that MEAM1 was the predominant whitefly species in soybean in most sampling sites, while MED was found colonizing soybean plants in open field conditions alone and/or with MEAM1 in several places. Among the tested insects, MED species was mostly detected harbouring the facultative endosymbiont Hamiltonella. We also detected cowpea mild mottle virus (CPMMV) infecting soybean plants. Transmission assays demonstrated that MED species was more efficient transmitting CPMMV from beans to beans and from soybean to soybean plants than MEAM1, while MEAM1 was more efficient in transmitting the virus from soybean to bean than MED. Performance assays indicated that adult emergence of both species on CPMMV-infected soybean plants was higher when compared with the emergence on healthy plants. Moreover, nonviruliferous MED and MEAM1 adults preferred to settle more often on healthy plants, while viruliferous adults settled more often on CPMMV-infected soybean plants. As MED has already been found in soybean open fields in São Paulo and Paraná States, Brazil, and it is a good vector of CPMMV, we anticipate problems related to whitefly management and to increase in the incidence of the virus in soybean.  相似文献   

EPSPS duplication and mutation involved in glyphosate resistance in the allotetraploid weed species Poa annua L.     

Caio Augusto De Castro Grossi Brunharo  Sarah Morran  Katie Martin  Marcelo L Moretti  Bradley D Hanson 《Pest management science》2019,75(6):1663-1670

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Assessing the impact of climate change on the worldwide distribution of Dalbulus maidis (DeLong) using MaxEnt     

Paulo A Santana  Lalit Kumar  Ricardo S Da Silva  Jardel L Pereira  Marcelo C Picano 《Pest management science》2019,75(10):2706-2715

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Gastric coccidiosis in Thoracocharax stellatus caused by Goussia guamaensis n. sp. (Apicomplexa: Eimeriidae) from the Amazon region     

Marcelo F. da Silva  Rebeca S. de Araújo  Diehgo T. da Silva  Osimar Sanches  Igor Hamoy  Edilson Matos 《Journal of fish diseases》2019,42(6):905-912

Ninety‐seven specimens of spotfin hatchetfish, Thoracocharax stellatus, an ornamental freshwater species from the Amazon basin, were captured in the basin of the Guamá River in the municipality of Belém, in northern Brazil, and analysed for coccidiosis infection. Overall, 26 of the specimens were infected by apicomplexan parasites of the genus Goussia, with unsporulated forms being found in the gastric epithelium and sporulated forms in the intestinal lumen. The spheroid oocysts (mean diameter: 13.2 ± 1.7 μm) have four elliptical sporocysts. A partial sequence of the SSU rDNA of the new species was obtained, which contained 1,121 base pairs, with 43.8% guanine + cytosine (G + C), and the bases distributed as follows: A = 28.1%, C = 18.3%, G = 25.5% and T = 28.1%. The combined analysis of the morphometric and phylogenetic evidence confirmed that the specimens represented the genus Goussia and were allocated to a new species, Goussia guamaensis n. sp., which is described here.  相似文献   

Population structure of the critically endangered Brazilian guitarfish Pseudobatos horkelii (Rhinobatidae) revealed by double-digest restriction site-associated DNA sequencing     

Yan Torres  Matheus Marcos Rotundo  Marcelo Vianna  Patricia Charvet  Vicente Vieira Faria  Claudio Oliveira  Fausto Foresti  Vanessa Paes Cruz 《水产资源保护:海洋与淡水生态系统》2024,34(1):e4035

Agronomic,nutritive value,reproductive, cytogenetic,and molecular aspects of Paspalum accessions: Contribution to the development of new forage cultivars     

Frederico de Pina Matta  Alessandra Pereira Fávero  Bianca Baccili Zanotto Vigna  Marisa Toniolo Pozzobon  Sergio Raposo de Medeiros  Waldomiro Barioni Júnior  Marcelo Mattos Cavallari 《Grass and Forage Science》2023,78(1):101-118

This study investigated the agronomic, nutritive value, cytogenetic, molecular, and reproductive aspects of twenty-five Paspalum accessions to support breeding programs. The agronomic aspects and the nutritive value were evaluated in a randomized block experimental design with four replications, and with monthly repeated measures for 2 years. For the molecular characterization 16 microsatellite markers developed for different Paspalum species in previous works were used. The number of chromosomes was determined by visualizing the meiotic cells and the DNA content was determined by flow cytometry. The embryo sac clarification technique was used to characterize the reproductive system. T he results indicated genetic variability for all the variables related to yield and nutritive value. For almost all variables, the values of Paspalum accessions were higher than those of the commercial cultivars Megathyrsus maximus cv. Tanzania and Urochloa brizantha cv. Marandu, used as references. However, the accessions showed in vitro dry matter digestibility values lower than the mean value presented by the references. The groups formed in the molecular analysis showed correspondence to the groups observed in the principal components analysis, considering the variables related to yield and nutritive value. Most accessions were revealed to be tetraploid with 40 chromosomes and most accessions belonging to the Virgata group displayed a sexual embryo sac structure . Some crosses can be indicated for genetic breeding, due to a superior genomic affinity between the accessions and the higher probability of heterosis in the progeny. These results provide subsidies to genetic plant breeding programs that use these accessions.  相似文献   

Cloning of a Genomic DNA Encoding Caffeoyl-coenzyme A 3-<Emphasis Type="Italic">O</Emphasis>-methyltransferase of Citrus     

Alfredo Gryciuk ALMEIDA  Shinji TSUYUMU 《Journal of General Plant Pathology》2000,66(2):144-148

In recent studies, we found that Apll (a PthA homologue) bound to three citrus proteins. Amino acid sequence analysis revealed that one of the target proteins was homologous to that of S-adenosyl-l-methionine : trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAMT), an enzyme which is specific for the substrate trans-caffeoyl-CoA and catalyzes the synthesis of trans-feruloyl-CoA. From the consensus nucleotide sequences of CCoAMT genes, primers were chosen for PCR amplification of this gene from citrus total DNA. Two selected DNA fragments of 1.0 kb and 2.0 kb were obtained. These fragments were used as the probe to screen a citrus library. One clone, pCCl00, contained a 1.0-kb SalI fragment that hybridized to the probes. The nucleotide sequence of this fragment was determined in both directions. In this fragment, there was an open reading frame of 232 amino acids interrupted by an intron of 106 nt, and the deduced amino acid sequence had 95.9% homology to tobacco CCoAMT. Southern blot analysis of total citrus DNA showed that four EcoRI fragments hybridized to the probes, suggesting the presence of more than one copy of CCoAMT in the citrus DNA. Received 4 November 1999/ Accepted in revised form 26 November 1999  相似文献   

ZigBee-based wireless sensor network localization for cattle monitoring in grazing fields   总被引：7，自引：0，他引：7  

Juan Ignacio Huircán  Héctor Young  Jaime Bustos  Marcelo Toneatti 《Computers and Electronics in Agriculture》2010,74(2):258-264

This paper presents the design of a localization scheme in wireless sensor networks (WSN) for cattle monitoring applications in grazing fields. No additional hardware was required for distance estimation since they were performed using the link quality indication (LQI), which is a standard feature of the ZigBee protocol. The ratiometric vector iteration (RVI) algorithm was implemented and modified to work with LQI measurements instead of the usual received signal strength indication (RSSI). Experimental results show acceptable localization performance given the requirements of usual cattle monitoring applications at low cost and low power consumption.  相似文献   

Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares     

Carolina Paula BIANCHI  Santiago BRUNO  Ignacio VIDELA DORNA  Edgardo RODRÍGUEZ  Marcelo Alfredo ABA 《Journal of Equine Science》2022,33(1):1

In study I, plasma progesterone concentrations were evaluated in anoestrous mares that received an intravaginal progesterone release device (IPRD) for 10 days. Mares were divided into 3 groups based on the dosage of progesterone (0 g, n=3; 1.38 g, n=5; and 1.9 g, n=5). No statistical differences were found in plasma progesterone concentrations between the two doses tested. In study II, the effects of a protocol based on a short program of artificial light combined with an IPRD containing 1.38 g of progesterone on oestrous behaviour and onset of ovulation were evaluated. IPRDs were inserted into 31 late transitional mares (10 days of treatment). The mares were divided into a control group (n=9, IPRD with 0 g of progesterone) and two treatment groups (T1, n=10, IPRD with 0 g of progesterone and artificial light; T2, n=12, IPRD with 1.38 g of progesterone and artificial light). The percentages of mares in heat within the first 14 days after treatment were 100%, 70%, and 100% in the control, T1, and T2 groups, respectively (P=0.097), and their ovulation rates were 44%, 60%, and 100%, respectively (P≤0.01). In conclusion, a protocol based on artificial light and an IPRD containing 1.38 g of progesterone for 10 days could be considered to advance the first ovulation of the year in late transitional mares, as it ensures a higher rate of ovulation within the first 14 days after treatment.  相似文献   

Intrauterine inoculation of a bacteria-free filtrate of streptococcus zooepidemicus in clinically normal and infected mares     

Marcelo A. Couto  John P. Hughes 《Journal of Equine Veterinary Science》1985,5(2):81-86

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