Flow cytometric characterization of peripheral blood leukocyte populations of 3 neotropical snake species: Boa constrictor,Bothrops jararaca,and Crotalus durissus          下载免费PDF全文

Marcelo P. N. Carvalho  Nicolle G. T. Queiroz‐Hazarbassanov  Cristina O. Massoco  Silmara Rossi  Sávio S. Sant'Anna  José L. Catão‐Dias  Kathleen F. Grego 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(2):271-280

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Québec. Isolation of Staphylococcus felis from cases of external otitis in cats          下载免费PDF全文

Higgins R  Gottschalk M 《The Canadian veterinary journal. La revue veterinaire canadienne》1991,32(5):312-313

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Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats     

Juliana Paula Martins Alves  Csar Carneiro Linhares Fernandes  Rafael Rossetto  Caroline Pessoa da Silva  Iolly Tbata Oliveira Marques Galvo  Marcelo Bertolini  Davide Rondina 《Reproduction in domestic animals》2019,54(9):1206-1216

The aim of this study was to verify the effect of the energy source for a short‐term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non‐dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full‐fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous‐synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40‐mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.  相似文献   

Lectin histochemical pattern on the normal and cystic ovaries of sows     

Marcelo HAF Mendes  Maria HB Pinto  Eduardo J Gimeno  Claudio G Barbeito  Fabiano JF de Sant'Ana 《Reproduction in domestic animals》2019,54(10):1366-1374

The lectin histochemical pattern (LHP) was characterized and compared in normal and cystic ovaries of sows. Six biotinylated lectins (PNA, SBA, WGA, RCA‐1, DBA and UEA‐1) were used on tissue sections. In the normal ovaries, the reaction to UEA‐1 and SBA was mild to moderate in mesothelial and endothelial cells. RCA‐1 staining was mild to moderate in theca interna of growing follicles, corpora luteum and mesothelium. In addition, this lectin presented strong reaction in endothelial cells, granulosa cells of atretic follicles, zona pellucida of growing follicles and plasma. DBA showed strong intensity in mesothelial and endothelial cells. There was mild to moderate reactivity to WGA in granulosa cells, corpus luteum and theca interna of follicles in development, and moderate in zona pellucida, in granulosa cells of atretic follicles and mesothelium. PNA staining was mild to moderate in oocytes and in the adventitia and media of medullary arteries. Changes in the LHP of the cystic ovaries were noted; however, there were no differences in these findings between the follicular and luteinized cysts. UEA‐1 reactivity in the cystic ovaries was moderately reduced in the mesothelial and endothelial cells, whereas there was mild reduction in the DBA staining in the granulosa cells. Reaction to RCA‐1 and WGA in the cysts also was decreased in theca interna, zona pellucida and granulosa cells of atretic follicles. Furthermore, endothelium and theca interna in the cystic ovaries presented mild reduction of marcation to SBA, whereas there was decreased reactivity to PNA in the oocytes and adventitia and media layers of the medullary arteries. The results of the current study show that cysts modify the LHP in swine ovaries. These changes of glycoconjugates in many ovarian structures could modify diverse process and may be one of the reasons for decreased fertility in sows.  相似文献   

Morphological description of male genital organs of Marca's marmoset (Mico marcai)          下载免费PDF全文

Gustavo Henrique Lima de Siqueira  Felipe Ennes Silva  Marcelo Ismar Silva Santana 《Anatomia, histologia, embryologia》2018,47(4):372-384

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Response to Letter to the Editor: Do we have to redefine lameness in the era of quantitative gait analysis          下载免费PDF全文

S. Adair  M. Baus  J. Belknap  R. Bell  M. Boero  C. Bussy  F. Cardenas  T. Casey  J. Castro  W. Davis  M. Erskine  R. Farr  T. Fischer  B. Forbes  T. Ford  R. Genovese  R. Gottschalk  M. Hoge  C. Honnas  G. Hunter  J. Joyce  A. Kaneps  K. Keegan  J. Kramer  C. Lischer  J. Marshall  M. Oosterlinck  P. Radue  R. Redding  S. K. Reed  M. Rick  E. Santschi  M. Schoonover  M. Schramme  John Schumacher  R. Stephenson  R. Thaler  J. Vedding Neilsen  D. A. Wilson 《Equine veterinary journal》2018,50(3):415-417

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Computer tomographic imaging in 4 dogs with primary nasal canine transmissible venereal tumor and differing cellular phenotype          下载免费PDF全文

Javier Ojeda  Marcelo Mieres  Francisco Soto  Verónica Arnes  Enrique Paredes  María Navarrete 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1172-1177

Primary nasal canine transmissible venereal tumor (CTVT) without genital affection is uncommon. The aim of this report was to describe the primary nasal CTVT findings and CT staging in 4 dogs with different cytological phenotypes. Three male dogs and 1 bitch were evaluated for their chronic histories of sneezing, snoring, mucopurulent nasal discharge and nasal deformation. Cytological examination of nasal secretions suggested CTVT, confirmed by histopathological examination and LINE‐1/c‐myc. Males had the plasmacytoid phenotype of CTVT, and the bitch had the lymphocytoid phenotype. CTVT were staged based on the CT findings using modified Adams staging system. The bitch was classified as stage 1, 2 males were classified as stage 3 and 1 male as stage 4. All dogs had a complete tumoral remission after chemotherapy. Plasmacytoid phenotype was identified in cases with most important damage of the nasal cavity. However, the cytological type did not affect the response to chemotherapy.  相似文献   

Anatomical and physiological evidence of white blush on baby carrot surfaces     

Adriano do N. Simes  Marília C. Ventrella  Celso L. Moretti  Marcelo A.G. Carnelossi  Rolf Puschmann 《Postharvest Biology and Technology》2010,55(1):45-52

This study characterizes dehydration and white blush processes, structural alterations and synthesis of phenolic compounds (lignin and suberin), in relation to development of white blush on baby carrot surfaces. Carrots were minimally processed as baby carrots and kept on polypropylene trays with or without polyvinyl chloride (PVC) film at 5 ± 2 °C, 90 ± 5% RH. During storage, baby carrots that were not wrapped with PVC film were rehydrated 1, 1.5, 15 and 17 h after minimal processing. Fresh-cut baby carrots were evaluated for white blush index, sensory analysis (visual scores), fresh matter loss, phenylalanine ammonia-lyase (PAL) activity and structural and histochemical changes. Increases in white blush index and subjective visual scores on the carrot surface occurred in the first hours, when the material was kept on trays without PVC film and after 3 and 6 d, when stored on trays covered with PVC film. Visual assessment of white blush resulted in a more accurate assessment than instrumental evaluation because it allowed the perception of minor differences between distinct white blush levels, especially at the tips. Hydrating baby carrot surfaces for 1 and 1.5 h after processing allowed partial absorption of water by tissues and the orange color was reestablished on the surface. Fifteen hours after processing, even after rehydration, the color did not return to the original orange standard. The rapid increase in PAL activity in the secondary phloem of baby carrots, compared to that of whole carrots, suggested a physiological response as a result of abrasion. Dehydration and structural alterations of the more superficial cell layers were the main causes of white blush in baby carrots that was not related to lignin accumulation, but rather to synthesis of non-structural phenolic compounds.  相似文献   

Performance of dual and triple fungicide premixes for managing soybean rust across years and regions in Brazil: A meta-analysis     

Jhonatan P. Barro  Kaique S. Alves  Cláudia V. Godoy  Alfredo R. Dias  Carlos A. Forcelini  Carlos M. Utiamada  Edson R. de Andrade Júnior  Fernando C. Juliatti  Fernando J. Grigolli  Heraldo R. Feksa  Hercules D. Campos  Ione C. P. V. Chaves  Ivan P. Araújo Júnior  João Mauricio T. Roy  José Nunes Júnior  Luana M. R. Belufi  Luciana C. Carneiro  Luís H. C. P. Silva  Marcelo G. Canteri  Marcio M. Goussain Júnior  Marina Senger  Maurício C. Meyer  Moab D. Dias  Mônica A. Müller  Mônica C. Martins  Mônica P. Debortoli  Nédio R. Tormen  Silvânia H. Furlan  Tiago F. Konageski  Valtemir J. Carlin  Wilson S. Venâncio  Emerson M. Del Ponte 《Plant pathology》2021,70(8):1920-1935

Soybean rust in Brazil is currently controlled with several commercial fungicide premixes composed of demethylation inhibitors (EPOXiconazole, CYPRoconazole, PROThioconazole, TEBUconazole), quinone-outside inhibitors (AZOXystrobin, TriFLoXystrobin, PYRAclostrobin, PICOxystrobin), and succinate demethylation inhibitors (BENZovindiflupyr, BIXaFen, FLUXapyroxad). Here, we summarize the performance of eight premixes evaluated in 177 cooperative trials conducted in 46 locations across 10 states from 2015 to 2020. All fungicide treatments were sprayed three times starting at R1/R2. Percentage control (, %), from back-transforming meta-analytic estimates of the log of the ratio, ranged from 56.2% (PICO + CYPR) to 76.8% (BIXF + TFLX + PROT). Estimates of mean yield difference (, kg/ha) between fungicide-treated and untreated plots were greatest for BIXF + TFLX + PROT (1,080) followed by PICO + BENZ (1,010), PYRA + EPOX + FLUX (981.5), AZOX + BENZ (910), TFLX + PROT (891), PICO +TEBU (682), TFLX + CYPR (646), and PICO + CYPR (600). Significant declines in both and in as little as 4 years were detected for AZOX + BENZ (35.3%; 550 kg/ha) and PICO + BENZ (15.5%; 359.8 kg/ha). Variance in was reduced by the inclusion of baseline severity as covariate. In trials where baseline disease was ≥70%, yield was 250 kg/ha greater compared to areas with low baseline disease. Disease control and yield response were generally greater in the south-east, where the frequency of profitable scenarios was 30% higher on average than in the north-west. Results of this meta-analysis are critical for supporting decisions during planning of fungicide programmes.  相似文献   

Supplementation of citric acid in plant protein‐based diets for juvenile tambaqui,Colossoma macropomum     

Marcelo dos S. Nascimento  Bruno O. de Mattos  Mrcia R. F. M. Bussons  Adriano T. de Oliveira  Ariany R. da S. Liebl  Thaís B. Carvalho 《Journal of the World Aquaculture Society》2021,52(1):231-243

We evaluated citric acid supplementation (0, 10, 20, and 30 g/kg) in plant protein‐based diets for juvenile tambaqui, Colossoma macropomum. In Experiment 1, fish (n = 160; 27.56 ± 2.73 g) were distributed in 310 L tanks (n = 16) for 80 days. Zootechnical performance, hematological parameters, and mineral composition (muscle and bones) did not differ significantly (p > .05). The inclusion of citric acid‐reduced pH of diets (p = .001) and stomach (p = .035). In Experiment 2, fish (n = 60; 48.00 ± 1.98 g) were distributed in fecal collection aquaria (200 L) for 21 days. The following were observed: a linear effect for the crude protein (p = .0004) and crude fat (p = .0037) digestibility coefficients; a quadratic effect for crude energy (p = .0037) and dry matter (p = .0004); an optimal supplementation level of 18.5 g/kg. Calcium (p = .0060) and magnesium (p = .0222) showed a linear effect, while phosphorus (p = .0001) had a quadratic effect with the 20 g/kg optimum supplementation level. To conclude, the supplementation of this organic acid in diets does not negatively affect fish nutritional status, health or welfare, and increases nutrient and mineral availability.  相似文献