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61.
André MR Adania CH Teixeira RH Allegretti SM Machado RZ 《Journal of zoo and wildlife medicine》2011,42(1):139-143
Large and small piroplasms have been observed in the blood smears of various wild carnivores, but few studies utilizing molecular characterization have been done. The goal of this present study was to investigate the presence of Babesia sp. by molecular and serologic techniques in exotic and neotropical carnivores maintained in captivity at Brazilian zoos. Blood and sera samples were collected from 146 Brazilian wild felids, 21 exotic felids, 1 genet (Genetta tigrina), 3 European wolves (Canis lupus), and 94 Brazilian wild canids in Brazilian zoos in the S?o Paulo and Mato Grosso states and in the Federal District. A total of 53 wild felids (31.74%) and 10 wild canids (10.31%) were seropositive for Babesia canis by Indirect Fluorescent Antibody Test (IFAT). Antibodies were detected in ocelots, little-spotted cats, margays, pampas cats, jaguars, pumas, jaguarundis, crab-eating foxes, and bush dogs. Babesia sp. DNA, with high similarity to B. leo, was detected in one pampas cat and one genet. 相似文献
62.
Sampling strategy to develop a core collection of Uruguayan maize landraces based on morphological traits 总被引：4，自引：0，他引：4
Core collections were suggested to improve germplasmutilization. A core collection is a subset chosen to represent thediversity of a collection with a minimum of redundancies. Becausediversity is distributed between and within groups with differentdegrees of organization, an adequate classification of accessionsinto related groups should be performed prior to the selection of acore collection. Different classification strategies for theUruguayan Maize Collection were compared, and the best one was usedto select a core collection. The following classification strategieswere compared following a multivariate approach using the availablemaize data base: i) racial classification, ii) geographicorigin (south and north of the country), and iii) acombination of kernel type and geographic origin. The third optionwas considered the best classification rule, since it takes intoaccount two points which are closely related to the distribution ofdiversity: genotypic composition and geographic origin. The followingfive groups were identified in the collection: a) pop, b)floury, c) dent, d) southern flint-semiflints, ande) northern flint-semiflints. Eight core collections,each of 90 accessions, were selected, using different strategies toweight the groups in the core and to select the accessions from thegroups. The P, C, and L strategies were used and combined with eitherrandom selection within the group or the Relative Diversity method.Two samples of 90 accessions were obtained at random withoutconsidering the classification. The Relative Diversity methodcombined with the L strategy produced the best core collection, as itretained the highest percentage of the ranges for the 17 variablesincluded in the analysis. On average, 91% of the ranges wereretained in the core, confirming its representativeness. 相似文献
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