Frank Wolf profile. The congressman with his hand on science's purse strings     

Mervis J  Kintisch E 《Science (New York, N.Y.)》2005,310(5753):1417-1418

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Ecology. Israeli controversy blossoms over protecting Gilboa iris     

Kintisch E 《Science (New York, N.Y.)》2005,308(5726):1251

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Nuclear waste. Academy gets the word out after tussle with agency     

Kintisch E 《Science (New York, N.Y.)》2005,308(5720):335

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DOE weapons lab. Los Alamos appoints interim director     

Kintisch E 《Science (New York, N.Y.)》2005,308(5724):936

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Bioethics. Anticloning forces launch second-term offensive     

Kintisch E 《Science (New York, N.Y.)》2005,307(5716):1702-1703

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U.S. military installations. Base commission alters Pentagon's wishes on labs     

Kaiser J  Kintisch E 《Science (New York, N.Y.)》2005,309(5740):1472

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Nuclear power. Congress tells DOE to take fresh look at recycling spent reactor fuel     

Kintisch E 《Science (New York, N.Y.)》2005,310(5753):1406

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Participation of Rhipicephalus sanguineus (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis     

Coutinho MT  Bueno LL  Sterzik A  Fujiwara RT  Botelho JR  De Maria M  Genaro O  Linardi PM 《Veterinary parasitology》2005,128(1-2):149-155

The vectorial competence of the tick Rhipicephalus sanguineus is discussed in relation to the epidemiology of canine visceral leishmaniasis, taking into account its strict association with dogs and the low indices of natural infection presented by its known vector, the phlebotomine sand fly Lutzomyia longipalpis. In order to evaluate natural infection by Leishmania chagasi and the infectivity of these parasites in the tick, 39 specimens (6 females, 11 males and 22 nymphs) of R. sanguineus were removed from 21 dogs showing diverse symptoms of zoonotic visceral leishmaniasis (ZVL). Six ticks (15.4%) gave positive results for the genus Leishmania using the PCR technique. To determine the infectivity of the parasites, 36 hamsters were inoculated orally and peritoneally with macerates of ticks removed from nine dogs symptomatic for visceral leishmaniasis. After 6 months the hamsters were sacrificed and necropsied. Serum was removed for IFAT, as well as spleen and liver fragments to make imprint smears and for PCR. Eight (88.9%) of these dogs presented ticks that were infective for 14 hamsters (41.2%), 12 (85.7%) of them infected peritoneally and two (14.3%) orally. PCR revealed 27 smears (40.9%) to be positive, 20 (62.5%) of them infected peritoneally and seven (20.6%) orally. IFAT showed 14 positive animals (41.2%). Based on these findings, we suggest that the vectorial capacity of R. sanguineus for L. chagasi should be evaluated further, opening new perspectives in the epidemiology of ZVL.  相似文献   

Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures     

Marcelino I  Veríssimo C  Sousa MF  Carrondo MJ  Alves PM 《Veterinary microbiology》2005,110(1-2):87-96

Ehrlichia ruminantium is the causative agent of Heartwater, a fatal tick-borne disease affecting ruminants in African countries and West Indies and can be used as an inactivated vaccine for wild and domestic animals. In order to improve E. ruminantium production yields we characterize E. ruminantium growth kinetics in terms of duplication time, maximum production yield, and peak of infectivity. After a 24 h period for E. ruminantium attachment/internalization and a lag phase of 12 h, the exponential growth occurred within 36-108 h post-infection (hpi) with a net increase of up to 2.2 orders of magnitude. Maximum E. ruminantium infectivity was observed at 120 hpi and was defined as the best time of harvesting (TOH) for propagation of E. ruminantium cultures. This study showed that considering the quality constraint of the final product (E. ruminantium vaccine), the E. ruminantium suspension should be harvested at 113 hpi. Overall, the characterization of E. ruminantium progression through the average infection cycle, not only can contribute to the maximization of E. ruminantium production yield, with important consequences for the large scale production and utilization of an inactivated Heartwater vaccine, but also to elucidate growth mechanisms of some of the other ehrlichial species, with emerging impact in human and animal health.  相似文献   

Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy     

Pigatto JA  Andrade MC  Laus JL  Santos JM  Brooks DE  Guedes PM  Barros PS 《Veterinary ophthalmology》2004,7(3):205-208

The objective of this study was to examine the endothelial surface morphology and to perform morphometric analysis of the corneal endothelial cells of Yacare caiman (Caiman yacare) using scanning electron microscopy. Morphometric analysis with regard to polygonality, mean cell area, cell density and coefficient of variation of mean cell area was performed. Cell areas were measured using image analysis software. The normal corneal endothelium of Yacare caiman consisted of polygonal cells of uniform size and shape with interdigitations of the cell borders. Microvilli appeared as protrusions on the cellular surface. The average cell area was 270 +/- 24 microm(2) and the endothelial cell density was 3704 +/- 324 cells/mm(2). The coefficient of variation of cell area was 0.22. This study demonstrates that the Yacare caiman corneal endothelium is similar to those described in other vertebrates.  相似文献