Evaluation of a comparative pathogenesis between cancer-associated retinopathy in humans and sudden acquired retinal degeneration syndrome in dogs via diagnostic imaging and western blot analysis     

Gilmour MA  Cardenas MR  Blaik MA  Bahr RJ  McGinnis JF 《American journal of veterinary research》2006,67(5):877-881

OBJECTIVE: To evaluate dogs with sudden acquired retinal degeneration syndrome (SARDS) for evidence of pituitary gland, adrenal gland, and pulmonary neoplasia and antiretinal antibodies and to evaluate dogs with neoplasia for antiretinal antibodies. ANIMALS: 57 clinically normal dogs, 17 with SARDS, and 53 with neoplasia. PROCEDURE: Thoracic radiography, ultrasonography of adrenal glands, and contrast-enhanced computed tomography of pituitary glands were performed in 15 dogs with SARDS. Western blot analysis was performed on sera of all dogs; recoverin (23 kd) and arrestin (48 kd) retinal antibodies were used as positive controls. RESULTS: Neoplasia was not detected via diagnostic imaging in dogs with SARDS. Western blot analysis revealed bands in all dogs ranging from > 48 to < 23 kd. Prominent bands with equivalent or greater density than 1 or both positive controls at the 1:1,000 dilution, and present at the 1:3,000 dilution, were detected in 28% of clinically normal dogs, 40% of dogs with neoplasia, and 41% of dogs with SARDS. No bands in dogs with SARDS had a consistent location of immune activity, and none were detected at the 23-kd site. The area around the 48-kd site had increased immune activity in all 3 groups. CONCLUSIONS AND CLINICAL RELEVANCE: The etiology of SARDS in dogs does not appear to be similar to cancer-associated retinopathy in humans on the basis of absence of differential antibody activity against retinal proteins. Although dogs with SARDS often have clinical signs compatible with hyperadrenocorticism, neoplasia of the adrenal glands, pituitary gland, or lungs was not detected.  相似文献   

75 years of breeding pinto bean for resistance to diseases in the United States     

Henry Terán  Margarita Lema  David Webster  Shree P. Singh 《Euphytica》2009,167(3):341-351

Diseases are major production constraints of pinto bean despite 75 years of breeding. The objectives were to (1) assess progress made and (2) discuss future breeding strategies. Bean common mosaic virus (BCMV), anthracnose, rust, white mold, common blight, and halo blight and growth habit, seed weight, and seed coat darkening were evaluated for 52 cultivars released between 1944 and 2001. Sixteen cultivars were resistant to BCMV, 12 to race 53 of rust, five to race 73 of anthracnose, two to halo blight, and none to common blight and white mold. Four were resistant to two and none to three or more diseases. Four had Type I, ten Type II, and others Type III growth habit. Bill Z, Grand Mesa, Montrose, Olathe, Othello, Ouray and Pindak darkened slower in storage. Pintoba had the largest and Cahone smallest seed. An integrated breeding strategy is suggested for simultaneous improvement of resistance to multiple-diseases.  相似文献   

Interspecific variation of constitutive chemical compounds in <Emphasis Type="Italic">Pinus</Emphasis> spp. xylem and susceptibility to pinewood nematode (<Emphasis Type="Italic">Bursaphelenchus xylophilus</Emphasis>)     

María Menéndez-Gutiérrez  Margarita Alonso  Enrique Jiménez  Gabriel Toval  Pedro Mansilla  Adela Abelleira  Andrea Abelleira-Sanmartín  Raquel Díaz 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):939-953

Pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), was detected in Spain in 2008. This gives rise to serious concern, as the disease has caused severe environmental and economic losses in Portugal and in Asian countries. We studied interspecific variation in susceptibility to pine wilt disease and differences in constitutive chemical compounds in the xylem tissue of the seven pine species -P. canariensis, P. halepensis, P. pinaster, P. pinea, P. sylvestris, P. radiata and P. taeda. Two-year-old trees were inoculated with B. xylophilus. Water potential and nematode densities were measured for each species on specific dates; whereas, wilting symptoms were recorded weekly until the end of the assay. Chemical compounds in the xylem were determined prior to inoculation. Three different resistance groups can be established in terms of the pine species susceptibility to PWN: non- to slightly-susceptible (P. canariensis, P. halepensis, P. taeda and P. pinea), susceptible (P. pinaster and P. radiata), and highly-susceptible (P. sylvestris). Nematodes migrated downward to the roots in all seven species. Constitutive xylem nitrogen, total polyphenols, and marginally phosphorus were negatively correlated with mortality caused by PWN. The most susceptible species, Pinus sylvestris, presented high levels of constitutive lipid-soluble substances and low levels of manganese, pointing to a possible relation between these components and PWN susceptibility. The results suggest P. sylvestris, P. pinaster and P. radiata forests could be severely damaged by PWN in Spain and highlight how constitutive chemical compounds such as nitrogen might play a role in resistance mechanisms against PWN.  相似文献   

Oxytetracycline depletion and withdrawal time estimation following intraperitoneal administration in three species from Chilean salmon farming          下载免费PDF全文

Luis Norambuena‐Subiabre  Margarita P González  Sergio Contreras‐Lynch 《Aquaculture Research》2018,49(2):593-602

This study was conducted to evaluate the half‐time (T½) and withdrawal time (WT) of oxytetracycline (OTC) following intraperitoneal (i.p.) administration of OTC (24.8–34.7 mg/kg) in three farmed salmonid species, Coho salmon (Oncorhynchus kisutch), Rainbow trout (Oncorhynchus mikiss) and Atlantic salmon (Salmo salar). A detection technique in fish skin muscle through a high‐performance liquid chromatography (HPLC) with a diode array detector (DAD) was developed and validated. The depletion studies were carried out in controlled conditions (nine studies) and under field conditions (one study). The T½ and WT estimations from the skin muscle after the i.p. administration of OTC in salmonids appear to be longer than studies where the OTC was orally administrated. Furthermore, the OTC maximum concentration in muscle seems to be also higher in the i.p. treatment. Due to the prolonged WT following the i.p. OTC administration, cautions related to the salmon harvest time should be consider in order to prevent OTC traces in the final product.  相似文献   

Cloning and expression of buffalo active chymosin in Pichia pastoris     

Vallejo JA  Ageitos JM  Poza M  Villa TG 《Journal of agricultural and food chemistry》2008,56(22):10606-10610

To date, only recombinant chymosin has been obtained in its active form from supernatants of filamentous fungi, which are not as good candidates as yeasts for large-scale fermentations. Since Bos taurus chymosin was cloned and expressed, the world demand for this protease has increased to such an extent that the cheesemaking industry has been looking for novel sources of chymosin. In this sense because buffalo chymosin has properties that are more stable than those of B. taurus chymosin, it may occupy a space of its own in the chymosin market. The main objective of the present work was the production of active recombinant buffalo chymosin in the culture supernatant of Pichia pastoris . This yeast has demonstrated its usefulness as an excellent large-scale fermentation tool for the secretion of recombinant foreign proteins. RNA was extracted from the abomasum of a suckling calf water buffalo ( Bubalus arnee bubalis ). Preprochymosin, prochymosin, and chymosin DNA sequences were isolated and expressed into P. pastoris. Only the recombinant clones of P. pastoris containing the prochymosin sequence gene were able to secrete the active form of the chymosin to the culture supernatant. This paper describes for the first time the production of active recombinant chymosin in P. pastoris without the need of a previous in vitro activation. The new recombinant yeast strain could represent a novel and excellent source of rennet for the cheesemaking industry.  相似文献   

Pharmacokinetics of doramectin and ivermectin after oral administration in horses   总被引：5，自引：0，他引：5  

Pérez R  Cabezas I  Godoy C  Rubilar L  Muñoz L  Arboix M  Castells G  Alvinerie M 《Veterinary journal (London, England : 1997)》2002,163(2):161-167

A study was undertaken in order to compare plasma disposition kinetic parameters of doramectin (DRM) and ivermectin (IVM) in horses after oral administration. Ten crossbreed adult horses, clinically healthy, weighing 380-470 kg body weight (bw) were selected for study. Faecal examinations were performed to determine faecal parasite egg counts. Horses were allocated to two groups of five animals to provide an even distribution considering the variables sex, body weight and faecal egg count. Group I, were treated with an oral paste formulation of IVM at 0.2 mg/kg b/w and Group II, were treated with an oral dose of 0.2 mg/kg bw of DRM prepared as paste from the injectable formulation for oral administration. Blood samples were collected by jugular puncture between 0 h and 75 days post-treatment. Plasma was separated and later solid phase extraction and derivatization samples were analysed by high performance liquid chromatography (HPLC); a computerised kinetic analysis was carried out. Data were compared using the Mann-Whitney U-test.The mean plasma concentrations of DRM and IVM after oral administration in horses were detected until 30 and 20 days, respectively. Both drugs showed similar patterns of absorption and no significant differences were found for peak concentration, the time to peak concentration, or for absorptive half-life. The terminal elimination half-life was significantly (P<0.05) longer in the DRM treated group than for the IVM treated group. The differences observed in the elimination half-life explain the longer mean residence time and high values of area under the concentration time curve for the group treated with DRM, which are 30% higher than those of the IVM group. Considering its pharmacokinetics, tolerance and anthelmintic efficacy, the oral administration of DRM, could be an alternative to IVM for the control of parasitic diseases of horses.  相似文献   

Study of haemostatic disorders in experimentally induced leishmaniasis in Beagle dogs     

Josep Enric Valladares  Rafael Ruiz De Gopegui  Cristina Riera  Jordi Alberola  Montserrat Gállego  Yvonne Espada  Montserrat Portús  Margarita Arboix 《Research in veterinary science》1998,64(3):195

Haemostatic alterations in dogs experimentally infected with Leishmania infantumwere studied before and after therapy with meglumine antimonate. Haemostatic function tests including platelet count, collagen-induced platelet aggregation, prothrombin time, activated partial thromboplastin time, thrombin time, plasma fibrinogen determination, and serum fibrinogen/fibrin degradation products concentration were performed. In the course of infection and before treatment, moderate thrombocytopenia (P<0·00001) decreased collagen induced platelet aggregation (P=0·0003), prolonged thrombin time (P=0·0117) and increased fibrinogen/fibrin degradation products were observed. Statistically significant differences of plasma fibrinogen concentration, prothrombin time, and activated partial thromboplastin time were not encountered. Haemostatic parameters returned to normal values after therapy. The results indicate that Leishmania infection may impair haemostasis suggesting induction of disseminated intravascular coagulation (DIC), and that treating dogs in an early stage of infection may potentially avoid the possibility of developing an uncompensated DIC.  相似文献   

Detection of Feline leukemia virus in the endangered Iberian lynx (Lynx pardinus).   总被引：2，自引：0，他引：2  

Inés Luaces  Ana Doménech  Marino García-Montijano  Victorio M Collado  Celia Sánchez  J German Tejerizo  Margarita Galka  Pilar Fernández  Esperanza Gómez-Lucía 《Journal of veterinary diagnostic investigation》2008,20(3):381-385

Feline retroviruses are rarely reported in lynx species. Twenty-one Iberian lynx (Lynx pardinus) blood and tissue samples collected from Do?ana National Park and Los Villares (Sierra Morena) in southern Spain during 1993-2003 were analyzed by polymerase chain reaction to amplify nucleic acids from feline retroviruses. Six samples were positive for Feline leukemia virus (FeLV), but no samples tested positive for Feline immunodeficiency virus. The BLAST analysis indicated that 5 of the 6 sequences were closely related to FeLV strain Rickard subgroup A, whereas 1 sequence was identical to FeLV. To the authors' knowledge, this is the first report of FeLV in the endangered Iberian lynx.  相似文献   

Pharmacokinetics of albendazole sulfoxide enantiomers administered in racemic form and separately in rats   总被引：1，自引：1，他引：0  

Capece BP  Castells G  Godoy C  Arboix M  Cristòfol C 《Veterinary journal (London, England : 1997)》2008,177(2):297-299

The pharmacokinetic behaviour of albendazole sulfoxide (ABZSO) enantiomers was studied in rats after the oral administration of 10 mg/kg of rac-ABZSO, 5 mg/kg of (-)-ABZSO or 5 mg/kg of (+)-ABZSO. The disposition profiles of ABZSO enantiomers were similar in all treatments, but the calculated area under the curve for the (-)-ABZSO was higher in all cases compared with (+)-ABZSO. The results suggest that there is no chiral inversion of ABZSO enantiomers. After the administration of rac-ABZSO, 17.2% of the total dose was recovered in urine as albendazole ABZ (0.1%), albendazole sulfone ABZSO(2) (0.3%), albendazole 2-aminosulfone (ABZ-SO(2)NH(2)) (3.1%) and ABZSO (13.7%). The ratio (+) to (-) was similar in urine (1.6) and blood (1.7).  相似文献   

Pharmacokinetics of ivermectin in pregnant and nonpregnant sheep     

Pérez R  Palma C  Núñez MJ  Cox J  Arboix M 《Journal of veterinary pharmacology and therapeutics》2008,31(1):71-76

The plasma kinetic profile of ivermectin during the last trimester of pregnancy was studied in ewes after a single subcutaneous administration of 0.2 mg/kg body weight (BW). Sheep were randomly distributed into two groups. Ewes in group 1 (control, n=6) were left unmated, whereas in group 2 (pregnant, n=6) ewes were estrus-synchronized and mated with rams. Both groups were housed under similar conditions of management and feeding. At 120 days of pregnancy, both groups were given a subcutaneous injection of 0.2 mg/kg BW of ivermectin. Blood samples were taken by jugular puncture according to a fixed protocol between 1 h and 40 days post-treatment. After plasma extraction and derivatization, samples were analyzed by high performance liquid chromatography with fluorescence detection. A computerized pharmacokinetic analysis was performed, and the data were compared by means of the Student t-test. The results showed that plasma concentrations of ivermectin remained longer in the pregnant than in the control group. The mean values of pharmacokinetic parameters C(max), t(max), and area under the concentration-time curve (AUC) were similar for both groups of sheep. The mean residence time (MRT) values for the pregnant group (8.8+/-1.4 days) were higher (P<0.05) than those observed in the control group (5.3+/-1.9 days). It can be concluded that pregnancy increases the residence time of ivermectin in the plasma of pregnant sheep when it is administered subcutaneously.  相似文献