Evidence that active transmission of porcine cysticercosis occurs in Venezuela     

Cortez Alcobedes  Maria M.  Boggio  Gonzalo  Guerra  Ma. de Lourdes  de Gavidia  Magda Rodríguez  Rojas Reyes  Glenda C.  Ferrer  Elizabeth  Lares  Maria  Alviarez  Yenny  Harrison  Leslie J. S.  Parkhouse  R. Michael E. 《Tropical animal health and production》2010,42(3):531-537

There is a paucity of quantitative data on the status of porcine cysticercosis in Venezuela, information which is essential for understanding the level of disease transmission. This study was, therefore, conducted in a typical small rural community in Yaracuy State, Venezuela, where previous cases of human Taenia solium taeniasis/cysticercosis had been reported and where the free-ranging pig management practices and the lack of rudimentary sanitary facilities indicated an obvious risk for transmission of the disease. Serum samples from 52 village pigs were screened by enzyme-linked immunosorbent assays for anti-cysticercal antibodies (Ab-ELISA), using T. solium cyst fluid as the antigen and the HP10, monoclonal antibody-based, antigen trapping ELISA for parasite antigen (HP10 Ag-ELISA). Significantly, a high proportion of the animals (65.4% for the Ab-ELISA and 42.3% for the HP10 Ag-ELISA) were sero-positive. Five of the pigs, which were selected on that basis of positive tongue palpation, were killed for autopsy, and large numbers of viable cysticerci were found in the carcases. This unequivocal documentation of porcine cysticercosis in Venezuelan pigs presents clear evidence that T. solium is actively transmitted in Venezuela. Further detailed studies and implementation of appropriate control measures are therefore indicated.  相似文献   

Quality and Fertilizing Ability In Vivo of Sex‐Sorted Stallion Spermatozoa     

G Mari  G Rizzato  B Merlo  E Iacono  D Bucci  E Seren  C Tamanini  G Galeati  M Spinaci 《Reproduction in domestic animals》2010,45(2):331-335

Little information is available on the quality of stallion spermatozoa after sex sorting. The objectives of the present study were to assess the quality of sex‐sorted stallion spermatozoa and determine its fertilizing ability after hysteroscopic low dose insemination. Ejaculates from four stallions were collected and sorted by a MoFlo SX^® flow cytometer/sperm sorter. Before and after sorting, spermatozoa were evaluated for motility by Computer Assisted Sperm Analysis, viability (SYBR 14‐propidium iodide), mitochondrial function (JC‐1) and acrosomal status (fluorescein isothiocyanate Pisum sativum agglutinin conjugated). A fertility trial was carried out on four mares (seven oestrous cycles) by hysteroscopic insemination, depositing 5 × 10⁶ X‐bearing spermatozoa. Sex sorting resulted in a significant decrease (p < 0.001) in all motility characteristics. Sperm viability and percentage of spermatozoa with functional mitochondria were not affected by the sorting process, while the percentage of reacted spermatozoa was higher (p < 0.01) for non‐sorted than sorted spermatozoa. Pregnancy rate was 28.6% (2/7) after low dose hysteroscopic insemination. Only one pregnancy was carried to term with the birth of a healthy filly. In conclusion, despite the reduction in sperm motility, sex sorting did not impair stallion sperm viability and mitochondrial activity immediately post‐thaw; moreover, the sexed spermatozoa retained the ability to fertilize in vivo.  相似文献   

Biological control of postharvest diseases by microbial antagonists: how many mechanisms of action?     

A. Di Francesco  C. Martini  M. Mari 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(4):711-717

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Bacterial community composition of sediments from a milkfish Chanos chanos Forsskål farm          下载免费PDF全文

Sheila Mae S Santander – de Leon  Wolfgang Reichardt  Shielameh Peralta‐Milan  Maria Lourdes San Diego‐McGlone  Sharon Nonato Nuñal  Hongyi Wei  Takeshi Yoshikawa  Suguru Okunishi  Hiroto Maeda 《Aquaculture Research》2016,47(8):2569-2581

Bacterial communities in sediments underneath milkfish cages and adjacent off‐cage areas in Bolinao, Lingayen Gulf, West Philippine Sea were characterized using PCR‐denaturing gradient gel electrophoresis to assess influence of the fish farming to the sediments. The sediments in the fish cage (FC) area showed anoxic conditions [redox potential of ?165 to ?213 mV and acid‐volatile sulphide‐sulphur (AVS‐S) of 0.55–1.88 mg g^?1 (dry weight)] compared to the relatively oxic off‐cage area [redox potential of 72–81 mV and AVS‐S of 0.09–0.14 mg g^?1 (dry weight)]. The composition of the microbial populations revealed influence of the fish feed sedimentation with higher dominance of putative sulphate‐reducing bacteria (SRB) in the FC area. Compositions of the bacterial taxa showed dominance of the Bacteroidetes group and Deltaproteobacteria. The results suggest that the fish feeds created a new suitable environment, in which reductive microorganisms such as SRB, ferric‐ion‐reducing bacteria and nitrate‐reducing bacteria can thrive. Organic load from the feed and quantity of its leftover constituents may be the key factors that influence the structure of the bacterial community in the sediments of the milkfish farms.  相似文献   

Arbuscular mycorrhizal inoculation of micropropagated persimmon plantlets     

M. Marin  A. Mari  M. Ibarra  L. Garcia-Ferriz 《The Journal of Horticultural Science and Biotechnology》2013,88(5):734-738

Summary

Growth, development and nutrient status of micropropagated persimmon (Diospyros kaki) ‘Rojo Brillante’ in response to inoculation with two species of arbuscular mycorrhizal (AM) fungi were studied under nursery conditions. The species of AM fungi were Glomus intraradices and G. mosseae. Shoot growth depression and low root colonization percentage were observed to G. mosseae inoculation. Shoot and root growth enhancements were observed for plants colonised by G. intraradices. Inoculated plantlets with G. intraradices and high level of controlled-release fertilizer signi®cantly decreased shoot height in relation to treatment with low fertilization level. Furthermore, G. intraradices had unique effects on the mineral status of the persimmon plantlets. N and Ca were significantly increased and decreased, respectively, within the shoot tissue of plants colonised by G. intraradices. Early inoculation with G. intraradices appears to favour growth of micropropagated persimmon plantlets.  相似文献   

Epicatechin-3-O-(3″-O-methyl)-gallate content in various tea cultivars (Camellia sinensis L.) and its in vitro inhibitory effect on histamine release     

Maeda-Yamamoto M  Ema K  Monobe M  Tokuda Y  Tachibana H 《Journal of agricultural and food chemistry》2012,60(9):2165-2170

It has been reported that epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and the EGCG3″Me-rich green tea ( Camellia sinensis L.) cultivar 'Benifuuki' exhibit antiallergic effects. The objective of this study was to investigate the effect of various tea leaf catechins on histamine release from murine bone marrow mast cells (BMMC). At a dose of 50 μg/mL, the rank order of histamine release inhibition was observed to be epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) > gallocatechin-3-O-(3″-O-methyl)-gallate (GCG3″Me) > EGCG3″Me > gallocatechin-gallate (GCG) > catechin-gallate (CG) > EGCG > epicatechin-gallate (ECG) > epigallocatechin (EGC) > gallocatechin (GC). Of the various tea cultivars analyzed by HPLC, the greatest content of ECG3″Me was found in the third crop of 'Benifuuki' (1.05% dry weight). Moreover, ECG3″Me content was positively correlated with EGCG3″Me content in 'Benifuuki' tea leaves. In an assay of mixtures of ECG3″Me and EGCG3″Me, inhibitory activity (50 μg/mL in total) was increased as the content of ECG3″Me increased. This suggests that ECG3″Me might link to the antiallergic effect of 'Benifuuki' tea, as has been reported for EGCG3″Me.  相似文献   

The quality of cryopreserved sperm collected from feline caudal epididymides stored at room temperature     

Toyonaga M  Kaihara A  Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1395-1398

On the assumption that animals of wild feline species died in the field, caudal epididymal sperm were cryopreserved following storage of the feline epididymides at 20°C for 0-24 hr, and their qualities were observed. Compared to the qualities at 0 hr, no significant differences were noted following 12 hr of storage at 20°C. On comparison of the qualities between caudal sperm cryopreserved after 24 hr storage at 4°C and after 12 hr at 20°C followed by 12 hr storage at 4°C, no significant differences were noted. These findings suggest that the cryopreserved sperm collected from epididymides of dead animals might be useful for artificial insemination if cryopreservation was performed within 12 hr exposure to ambient temperature.  相似文献   

Use of a graft of demineralized bone matrix along with TGF-β1 leads to an early bone repair in dogs     

Servin-Trujillo MA  Reyes-Esparza JA  Garrido-Fariña G  Flores-Gazca E  Osuna-Martinez U  Rodriguez-Fragoso L 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1151-1161

Tibia fractures are common in small animal practice. Over the past decade, improvements to animal internal fracture fixation have been developed. TGF-β1 has been shown to be crucial in the development, induction and repair of bone. In present study, we investigate the effect of local application of a graft demineralized bone matrix (DBM) along with TGF-β1 in a model of open osteotomy induced experimentally in dogs. Tibia fracture was brought about by using an open osteotomy model in young male dogs. Fracture repair was evaluated by a histological and biochemical analysis. Collagen content, proteolytic activity and urokinase-type plasminogen activator (uPA) expression were analyzed at the end of the study. Radiographic analysis, alkaline phosphatase and hematological evaluation were performed weekly. At the fifth week, there was an improvement and restoration of bone architecture in animals treated with a graft containing TGF-β1 (5 ng/ml) compared with the control and graft groups, as was evidenced by the presence of an early formation of wide callus and bone regeneration. In addition, local application of TGF-β1 led to an increase in collagen and proteolytic activity. More immunopositive osteoclast and mesenchymal cells were found in bone tissue from animals treated with TGF-β1 as compared with the control group. No changes in alkaline phosphatase, hematological and clinical parameters were observed. This study shows that the combined use of DBM along with TGF-β1 is able to improve and accelerate the bone repair.  相似文献   

Pathology in practice. Severe chronic multifocal intramural fibrosing and eosinophilic enteritis, with occasional intralesional bacteria, consistent with feline gastrointestinal eosinophilic sclerosing fibroplasia. (FIESF)     

Sihvo HK  Simola OT  Vainionpää MH  Syrjä PE 《Journal of the American Veterinary Medical Association》2011,238(5):585-587

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Experimental model for removal of snake venom via hemoperfusion in rats     

Maximilian Estevan Oliveira  Jssica Campanholi  Roberta Lima Cavalcante  Felipe Silveira Moreno  Edson Hideaki Yoshida  Murilo Melo Juste Dini  lvio Franco de Camargo Aranha  Jos Carlos Cogo  Lourdes Dias  Stephen Hyslop  Denise Grotto  Valquíria Miwa Hanai‐Yoshida  Yoko Oshima‐Franco 《Journal of Veterinary Emergency and Critical Care》2020,30(3):286-294

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