Oxidant-antioxidant imbalance in horses infected with equine infectious anaemia virus     

Bolfă PF  Leroux C  Pintea A  Andrei S  Cătoi C  Taulescu M  Tăbăran F  Spînu M 《Veterinary journal (London, England : 1997)》2012,192(3):449-454

This study assesses the impact of equine infectious anaemia virus (EIAV) infection on the oxidant/antioxidant equilibrium of horses. Blood samples from 96 Romanian horses aged 1-25 years, were divided into different groups according to their EIAV-infection status, age, and time post-seroconversion. The effect of infection on oxidative stress was estimated by measuring enzymatic antioxidants (superoxide dismutase [SOD], glutathione peroxidase [GPx] and catalase), non-enzymatic antioxidants (uric acid and carotenoids), and lipid peroxidation (malondialdehyde [MDA]). Infection modified the oxidant/antioxidant equilibrium in the horses, influencing GPx and uric acid levels (P<0.05). Time post-seroconversion also contributed to oxidative stress imbalance, exhibiting a significant influence on both SOD and MDA concentrations in the blood (P<0.05). Animal age did not have a significant influence on oxidative stress. Recently infected horses (<1 year following seroconversion), and horses >5 years old, represented the most vulnerable category in terms of oxidative stress, followed by recently infected animals <5 years old. The results of this study are novel in implicating EIAV infection in the development of oxidative stress in horses.  相似文献   

Genetic structure and history of Swiss maize (<Emphasis Type="Italic">Zea mays</Emphasis> L. ssp. <Emphasis Type="Italic">mays</Emphasis>) landraces     

T. W. Eschholz  P. Stamp  R. Peter  J. Leipner  A. Hund 《Genetic Resources and Crop Evolution》2010,57(1):71-84

Between 1930 and 2003 with emphasis on the 1940s maize landraces (Zea mays L. ssp. mays) from all over Switzerland were collected for maintenance and further use in a new Swiss breeding program. The genetic relationship and diversity among these accessions stored in the Swiss gene bank is largely unknown. Our hypothesis was that due to the unique geographic, climatic, and cultural diversity in Switzerland a diverse population of maize landraces had developed over the past three centuries. The aims were to characterize the genetic diversity of the Swiss landraces and their genetic relationship with accessions from neighbouring regions as well as reviewing their history, collection, and maintenance. The characterization and grouping was based on analyses with ten microsatellite markers. Geographic, cultural, and climatic conditions explained a division in two distinct groups of accessions. One group consisted of landraces collected in the southern parts of Switzerland. This group was related to the Italian Orange Flints. The other group contained accessions from northern Switzerland which were related to Northern European Flints in particular German Flints. Historic evidence was found for a frequent exchange of landraces within the country resulting in a lack of region-specific or landrace-specific genetic groups. The relatively large separation between the accessions, indicated by high F _ST (0.42), might be explained partly by a bottleneck during the collection and maintenance phase as well as by geographical and cultural separation of north and south of the country. Due to the high genetic diversity, the accessions here are a potential resource for broadening the European flint pool.  相似文献   

Dr. Roger Gfeller: Aug 16, 1949–Nov 25, 2007     

Gary Stamp 《Journal of Veterinary Emergency and Critical Care》2008,18(1):1-1

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Foliage Age and Pollution Alter Content of Phenolic Compounds and Chemical Elements in Pinus nigra Needles     

Giertych  Marian J.  Karolewski  Piotr  de Temmerman  Ludwig O. 《Water, air, and soil pollution》1999,110(3-4):363-377

Changes of phenolics and chemical elements [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), manganese (Mn), iron (Fe), boron (B), copper (Cu), zinc (Zn), lead (Pb), cadmium (Cd), chromium (Cr), nickel (Ni) and cobalt (Co)] content in needles of black pine ( Pinus nigra) as dependent on age of needles (5–6 classes) and pollution were examined. The content of ortho-diphenols (o-dPh) and total phenols (TPh) was significantly higher at a polluted site than at a control one. It increased with age of needles at both sites. At the polluted site contents of N, K, Mg in black pine needles were lower and of Fe, Ni and F were higher than at the control site. An increase of content with age of needles at both sites was detected for Ca, Fe, B and F, and a decrease for N, P, K, Cu and Ni. The content of elements in different age classes of needles is connected with their mobility. The content of phenolics is negatively correlated with main nutrients and positively with some toxic elements.  相似文献   

Back-calculation method shows that within-flock transmission of highly pathogenic avian influenza (H7N7) virus in the Netherlands is not influenced by housing risk factors     

Marian E.H. Bos  Mirjam Nielen  Guus Koch  Annemarie Bouma  Mart C.M. De Jong  Arjan Stegeman 《Preventive veterinary medicine》2009,88(4):278-285

To optimize control of an avian influenza outbreak knowledge of within-flock transmission is needed. This study used field data to estimate the transmission rate parameter (β) and the influence of risk factors on within-flock transmission of highly pathogenic avian influenza (HPAI) H7N7 virus in the 2003 epidemic in The Netherlands. The estimation is based on back-calculation of daily mortality data to fit a susceptible-infectious-dead format, and these data were analysed with a generalized linear model. This back-calculation method took into account the uncertainty of the length of the latent period, the survival of an infection by some birds and the influence of farm characteristics. After analysing the fit of the different databases created by back-calculation, it could be concluded that an absence of the latency period provided the best fit. The transmission rate parameter (β) from these field data was estimated at 4.50 per infectious chicken per day (95% CI: 2.68–7.57), which was lower than what was reported from experimental data. In contrast to general belief, none of the studied risk factors (housing system, flock size, species, age of the birds in weeks and date of depopulation) had significant influence on the estimated β.  相似文献   

Feline leukaemia. ABCD guidelines on prevention and management     

Hans Lutz  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Herman Egberink  Tadeusz Frymus  Tim Gruffydd-Jones  Katrin Hartmann  Margaret J. Hosie  Albert Lloret  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Etienne Thiry  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):565-574

OverviewFeline leukaemia virus (FeLV) is a retrovirus that may induce depression of the immune system, anaemia and/or lymphoma. Over the past 25 years, the prevalence of FeLV infection has decreased considerably, thanks both to reliable tests for the identification of viraemic carriers and to effective vaccines.InfectionTransmission between cats occurs mainly through friendly contacts, but also through biting. In large groups of non-vaccinated cats, around 30–40% will develop persistent viraemia, 30–40% show transient viraemia and 20–30% seroconvert. Young kittens are especially susceptible to FeLV infection.Disease signsThe most common signs of persistent FeLV viraemia are immune suppression, anaemia and lymphoma. Less common signs are immune-mediated disease, chronic enteritis, reproductive disorders and peripheral neuropathies. Most persistently viraemic cats die within 2–3 years.DiagnosisIn low-prevalence areas there may be a risk of false-positive results; a doubtful positive test result in a healthy cat should therefore be confirmed, preferably by PCR for provirus. Asymptomatic FeLV-positive cats should be retested.Disease managementSupportive therapy and good nursing care are required. Secondary infections should be treated promptly. Cats infected with FeLV should remain indoors. Vaccination against common pathogens should be maintained. Inactivated vaccines are recommended. The virus does not survive for long outside the host.Vaccination recommendationsAll cats with an uncertain FeLV status should be tested prior to vaccination. All healthy cats at potential risk of exposure should be vaccinated against FeLV. Kittens should be vaccinated at 8–9 weeks of age, with a second vaccination at 12 weeks, followed by a booster 1 year later. The ABCD suggests that, in cats older than 3–4 years of age, a booster every 2–3 years suffices, in view of the significantly lower susceptibility of older cats.  相似文献   

Feline rabies. ABCD guidelines on prevention and management     

Tadeusz Frymus  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Herman Egberink  Tim Gruffydd-Jones  Katrin Hartmann  Margaret J. Hosie  Albert Lloret  Hans Lutz  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Etienne Thiry  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):585-593

OverviewRabies virus belongs to the genus Lyssavirus, together with European bat lyssaviruses 1 and 2. In clinical practice, rabies virus is easily inactivated by detergent-based disinfectants.InfectionRabid animals are the only source of infection. Virus is shed in the saliva some days before the onset of clinical signs and transmitted through a bite or a scratch to the skin or mucous membranes. The average incubation period in cats is 2 months, but may vary from 2 weeks to several months, or even years.Disease signsAny unexplained aggressive behaviour or sudden behavioural change in cats must be considered suspicious. Two disease manifestations have been identified in cats: the furious and the dumb form. Death occurs after a clinical course of 1–10 days.DiagnosisA definitive rabies diagnosis is obtained by post-mortem laboratory investigation. However, serological tests are used for post-vaccinal control, especially in the context of international movements.Disease managementPost-exposure vaccination of cats depends on the national public health regulations, and is forbidden in many countries.Vaccination recommendationsA single rabies vaccination induces a long-lasting immunity. Kittens should be vaccinated at 12–16 weeks of age to avoid interference from maternally derived antibodies and revaccinated 1 year later. Although some vaccines protect against virulent rabies virus challenge for 3 years or more, national or local legislation may call for annual boosters.  相似文献   

Impact of topography on cross-pollination in maize (Zea mays L.)     

Andr Vogler  Henri Eisenbeiss  Ingrid Aulinger-Leipner  Peter Stamp 《European Journal of Agronomy》2009,31(2):99-102

Understanding cross-pollination is important to achieve the coexistence of genetically modified (GM) and conventional maize (Zea mays L.); it is still not known whether topography favors or hinders cross-pollination. In 2005 and 2006, the effect of gradients of 3.4–6.8° on cross-pollination was investigated in the canton of Zurich, Switzerland. Cross-pollination was revealed by the presence of yellow-grains on a white-grain hybrid at distances up to 17.5 m from the yellow-grain pollen donor hybrid. The measurements of the inclination of the slope were based on aerial images data taken by an unmanned GPS/INS (Global Positioning System/Inertial Navigation System)-based and stabilized model helicopter, which delivered precise altitude-based data for sampling points at tassel height.The rate of cross-pollination increased significantly with decreasing altitude of the receptor field (r = 0.36–0.64). However, the effect seems to be weaker than that of other factors like wind direction and velocity.  相似文献   

Effect of environment on Pekin duck behaviour and its correlation with body condition on commercial farms in the UK     

Dr T.A. Jones  M.S. Dawkins 《British poultry science》2010,51(3):319-325

1.?Forty-six flocks of commercially-reared Pekin ducks were studied to determine the effects of housing system and environment on the behaviour of farmed ducks and its correlation with physical condition. Houses differed predominantly in their ventilation, drinking, feeding and brooding systems, and were indicative of systems currently in use in the UK.

2.?At 41 d of age ducks spent 1?5% of the time feeding, 6?7% drinking, 4?2% rooting and 15?5% dry preening. They spent large amounts of time relatively inactive, 43?5%, or performing comfort behaviours, 17%. On average 4?6% of their time was spent walking and only 1?8% wet preening.

3.?A greater proportion of the maximum number of ducks able to use the drinker at any one time used the trough; nipple use was least and Plasson use intermediate. The proportion of ducks wet preening was not affected by drinker type but increased with increasing drinker space (mm/bird).

4.?Duck behaviour was little affected by commercial production system and was influenced more by environment, age and physical condition. Activity at an older age incorporated more of the behaviours associated with thermal comfort (panting) and maintenance of plumage condition (dry and wet preening). These behaviours increased with increasing temperature, relative humidity and atmospheric ammonia. Poor walking ability was correlated to increased frequency of panting, reduced activity at the drinker, and longer resting bouts.  相似文献   

Studies on the Physiology of Hyacinth Bulbs Hyacinthus Orientalis L. I. Sugar Content and Metabolic Activities in Bulbs Exposed to Low Temperatures     

Joanna Nowak  Marian Saniewski  R. M. Rudnicki 《The Journal of Horticultural Science and Biotechnology》2013,88(4):383-390

Changes in the content of carbohydrates and in the activity of hydrolytic enzymes were studied in hyacinth bulbs exposed to low temperatures. It was found that the amount of insoluble carbohydrate initially present in the scales and buds of hyacinth bulbs decreased greatly after a prolonged period of low temperature treatment. This decrease was greater in rooted bulbs, held at a low temperature for 63 days in natural conditions, than in unrooted bulbs stored in a refrigerator for the same period. At the same time the levels of soluble mono- and oligosaccharides, and of soluble starch, increased in the scales and buds of chilled bulbs. These changes in sugar level were found to be correlated with changes in the activity of certain hydrolytic enzymes. The activity of these enzymes was higher in rooted bulbs than in those which had not rooted before treatment. The activity of hydrolytic enzymes was found to be considerably higher in the flower buds than in the scales.  相似文献