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141.
The efficacy of eight combinations of fluorogestone acetate (FGA, 20 or 40 mg as intravaginal device during 11 days), equine chorionic gonadotropin (eCG, 300 or 500 UI injected 48 hr before FGA removal) and prostaglandin F (cloprostenol, 0 or 50 μg injected 48 hr before FGA removal) aiming at induction and synchronization of oestrus and ovulation was evaluated during the anoestrus season in spring and during the breeding season in autumn in adult Beni Arouss goats. Oestrous behaviour was recorded between 12 and 60 hr after FGA removal. Blood samplings allowing to assess onset of the pre‐ovulatory LH surge and increase of progesterone as sign of an active corpus luteum were performed, respectively, between 20 and 60 hr and 3, 5, 8 and 15 days after FGA removal. No season‐related differences (spring vs. autumn) were observed for oestrous response (95% vs. 93%), pre‐ovulatory LH surge (94% vs. 84%) and luteal response after 3–8 and 11–15 days post‐treatment (respectively 92% vs. 66% and 92% vs. 98%). The onset of oestrus (21 [13–53] vs. 32 [12–54] hr) and LH surge (26 [20–60] vs. 38 [22–60] hr) occurred significantly later in autumn. FGA (40 vs. 20 mg) in autumn significantly delayed the onset of oestrus (36 [16–54] vs. 23 [12–47] hr) and LH surge (44 [26–58] vs. 33 [22–60] hr). Significant treatment‐related differences were recorded for onset of LH surge (earliest for 20 mg FGA, 300 IU eCG, 50 μg PGF) and onset of luteal phase (latest for 40 mg FGA, 300 IU eCG, 50 μg PGF). In conclusion, the hormone combinations tested appeared equally effective in terms of oestrous and ovulation rates. Season has influenced significantly the onset of oestrus and LH surge, and the high dose regimen of FGA delayed the ovarian response in autumn.  相似文献   
142.
143.
Corneal samples of cats with and without corneal diseases were screened with a pan‐Chlamydiales PCR and specific PCRs for Parachlamydia, Protochlamydia, Chlamydophila felis, Acanthamoeba and feline herpesviruses (FHV‐1). Several corneal samples tested positive for Parachlamydia and related Chlamydiales, indicating cat exposure to these intracellular bacteria.  相似文献   
144.
Regulators need a reliable, precise and easy to use tool for predicting the occurrence of pesticides in drain water and catchments in agricultural areas. Occurrence depends on a wide range of substance and site specific factors and this study presents a simple model built on the results from simulations of a detailed model system that does not neglect or omit any of these factors. A drainage dominated sub-catchment (0.03 km2) of the Lillebaek catchment (4.4 km2) on Funen, Denmark, represented by the catchment model MIKE SHE is considered. Detailed analyses have been made with respect to geological and hydrodynamic conditions as well as measurements of pesticide concentrations in ground and surface waters. Maximum concentrations in drain water, the time for reaching this concentration and the time interval for exceeding the limit value have been derived empirically from MIKE SHE simulations using degradation rates and sorption coefficients values for 37 pesticides included in the Danish PATE database. The relatively hydrophilic bentazon and hydrophobic pendimethalin are used as model pesticides for illustration. A simple tool applicable for a wide range of pesticides has thus been designed based on detailed analyses of a limited number of pesticides. The user requirements are degradation rates, sorption coefficients, application rates and regulatory limit values for the pesticides of interest.  相似文献   
145.
ABSTRACT

Irrigated rice cultivation is a major source of greenhouse gas (GHG) emissions from agriculture. Methane (CH4) and nitrous oxide (N2O) are emitted not only throughout the growing season but also in the fallow period between crops. A study was conducted for two transition periods between rice crops (dry to wet season transition and wet to dry season transition) in the Philippines to investigate the effect of water and tillage management on CH4 and N2O emissions as well as on soil nitrate and ammonium. Management treatments between rice crops included (1) continuous flooding (F), (2) soil drying (D), (3) soil drying with aerobic tillage (D + T), and (4) soil drying and wetting (D + W). The static closed chamber method was used to measure CH4 and N2O fluxes.

Soil nitrate accumulated and N2O was emitted in treatments with soil drying. Nitrate disappeared while ammonium gradually increased after the soil was flooded during land preparation, indicating net nitrogen mineralization. N2O emissions were highest in both transition periods in D + W (437 and 645 µg N2O m?2 h?1). Methane emissions were significant in only the F treatment. The highest global warming potential (GWP) in the transition between rice crops occurred in F, with CH4 contributing almost 100% to the GWP. The GWP from other treatments was lower than F, with about 60–99% of the GWP attributed to N2O emissions in treatments with soil drying. The GWP in the transition between rice crops represented up to 26% of the total GWP from harvest to harvest. This study demonstrates that the transition period can be an important source of GHG emissions with relative importance of CH4 and N2O depending on the soil water regime. Therefore, the transition period should not be disregarded when estimating GHG emissions for rice cropping systems.  相似文献   
146.

Background

In 2008, an outbreak of ovine footrot occurred in Norway. Dichelobacter nodosus isolates collected between 2008 and 2011 have been characterised. Isolates defined as virulent by the gelatin gel test (GG-test) were only found in sheep in Rogaland County, where the severe cases of footrot were registered. The majority (96%) of the virulent isolates belonged to serogroup A. It is suspected that they represent a newly introduced strain, and the aim of the present study was to investigate whether they are genetically similar. Sixty-one virulent isolates from sheep and 116 benign isolates from sheep, cattle and goats were included. Four GG-test virulent isolates from Danish sheep were also included. All isolates were genotyped by pulsed-field gel electrophoresis (PFGE) and by PCR for pgr variant determination.

Results

The Norwegian virulent isolates were assigned to 8 pulsotypes (PTs), while the benign isolates were assigned to 66 PTs. Thirty-seven (68.5%) of the 54, virulent, serogroup A isolates belonged to the same PT, and included isolates from 2008 through 2011. Isolates belonging to this PT were defined as the outbreak strain. The remaining virulent serogroup A isolates belonged to 4 PTs differing by ≤3 bands from the outbreak strain. Two virulent, Danish, serogroup A isolates differed by 2 bands from the Norwegian outbreak strain. All but 3 (95%) of the virulent isolates had the pgrA variant while 85% of the benign isolates had the pgrB variant.

Conclusion

This study provides evidence that the footrot outbreak in Norway in 2008 most likely was caused by new introduction and local spread of one virulent D. nodosus strain.  相似文献   
147.
148.
Indicators of piglet survival in an outdoor farrowing system   总被引:1,自引:0,他引:1  
Pre-weaning piglet mortality continues to be a major welfare and economic concern. In outdoor farrowing systems, there is a particular need to broaden breeding goals by incorporating selection for piglet survival to improve both productivity and welfare. This study aimed to identify behavioural and physiological survival indicators that are influential in outdoor systems and that could provide additional information for use when selecting for piglet survival. Data were collected from 511 piglets from Large White × Landrace × Duroc sows and Generalised Linear Mixed Models determined which indicators were most important for piglet survival in an outdoor system. With respect to prenatal mortality (surviving vs. stillborn piglets) high ponderal index (< 0.001) or body mass index (P < 0.001) in conjunction with being born earlier in the farrowing birth order (< 0.001) were the most important survival indicators. Birth weight (< 0.001) and rectal temperature 1 h after birth (= 0.032) were the most significant postnatal survival indicators. However survival indicators identified as important in indoor, conventional farrowing crates, such as landmark behaviours (latency to reach the udder, a teat and to suckle colostrum), were not influential in this system. These results highlight the importance of studying potential indicators of survival in alternative farrowing systems to the farrowing crate.  相似文献   
149.
This study was part of a larger study that addressed whether milk production levels affect health risks in dairy cows as influenced by genetic merit for milk yield and management factors. Plasma samples were collected from 80 Holstein Friesian heifers at 2 weeks pre-partum and at 4 and 8 weeks post-partum in a 2 × 2 × 2 factorial arrangement design with the factors breeding value for milk production (high or low), milking frequency (2 or 3 times a day) and feed energy density (high or low). The following parameters indicative of the oxidative status were measured in plasma: ferric reducing ability of plasma (FRAP), α-tocopherol level, glutathione peroxidase activity (GSH-Px), superoxide dismutase activity (SOD), thiobarbituric acid reactive substances (TBARS) concentration and paraoxonase activity (PON). There was a significant effect of time for FRAP, α-tocopherol, GSH-Px and SOD, indicating changes in the plasma oxidative status around parturition. A high merit for milk yield, three-times-daily milking and especially feeding the high energy feed all stimulated milk production level. However, the influence on oxidative status parameters was minimal. Only the FRAP value was significantly lower in the high compared to the low energy group. Cows seem to undergo some oxidative stress around parturition, which can make them more susceptible to disorders. However, there were no clear indications that breeding value, milk frequency or feed energy level jeopardizes heifers in a way oxidative stress became critical.  相似文献   
150.
蛋白粉的功能特性与蛋白粉的加工   总被引:1,自引:0,他引:1  
丹麦的研究结果表明了加工条件对蛋白粉特性的影响。加工厂可以根据这一信息采取措施来为特定的市场生产具有理想质量的蛋白粉。  相似文献   
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