An insertion/deletion variant of a thymine base in exon 2 of the porcine beta 3‐adrenergic receptor gene associated with loin eye muscle area     

Kensuke HIROSE  Mariko NAKAMURA  Tatsuya TAKIZAWA  Kazuo FUKAWA  Tetsuya ITO  Masayuki UEDA  Takashi SASAKI  Kazuaki TANAKA 《Animal Science Journal》2009,80(6):624-630

An insertion/deletion variant of a thymine base (T5 and T6) in exon 2 of porcine beta 3‐adrenergic receptor (ADRB3) gene has been described. In the current study, we made an association study between the ADRB3 polymorphisms and production traits in 735 Duroc pigs. The allele frequencies for the T5 and T6 alleles in our study population were 0.433 and 0.567, respectively. Any associations between ADRB3 genotype and average daily weight gain during test period, or backfat thickness and intramuscular fat content were not detected in either sex. However the size of the loin eye muscle area (EMA) was significantly associated with ADRB3 genotypes in gilts. T6‐homozygous gilts had a higher mean of EMA (40.6 ± 0.6 cm²) than T5‐homozygous (38.1 ± 0.4 cm², P = 0.002) and heterozygous (38.8 ± 0.3 cm², P = 0.034) gilts. This association was not detected in males. In addition, a multiple traits animal model best linear unbiased predictor (BLUP) analysis revealed that the T6‐homozygous genotype had positive effects on breeding value of EMA. Accordingly, we suggest that ADRB3 polymorphism has the potential to be an important genetic marker for prediction of EMA in Duroc pigs.  相似文献   

Recombinant expression and characterization of three group IB phospholipase A₂ isoforms in the red sea bream Chrysophrys major     

Yukichi  FUJIKAWA  Mariko  SHIMOKAWA  Shoko  WATANABE  Akiko  TAKEHARA  Satoshi  UCHIYAMA  Seiichi  HAYASHI  Muneharu  ESAKA  Hiroshi  MIYAMOTO  Mitsumasa  MANKURA  Noriaki  IIJIMA 《Fisheries Science》2003,69(6):1263-1270

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小分子热激蛋白基因在番茄花药中的转录   总被引：7，自引：0，他引：7  

刘箭  庄野真理子 《园艺学报》2001,28(5):403-406

 选择8 个耐热性不同的番茄品种种植在地处亚热带的日本最南部石垣市, 测定夏季高温环境下花粉粒的败育率, 并据此将其分成耐热型和热敏型品种, 分析高温胁迫下花药中4 类小分子热激蛋白的转录。结果表明, 番茄花药中含有组成性的细胞质小分子热激蛋白和线粒体小分子热激蛋白mRNA; 高温季节细胞质小分子热激蛋白和线粒体小分子热激蛋白基因转录明显; 热敏型番茄花药中小分子热激蛋白基因的转录高峰温度低于耐热型花药。  相似文献   

Technological development for the control of humidity conditioning performance of slit materials made from Japanese cedar     

Yuka Miyoshi  Mariko Furutani  Mayuko Ishihara  Shunichi Tai  Yuzo Furuta  Shuichi Kawai 《Journal of Wood Science》2015,61(6):641-646

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Three decades of avian research on the Bartlett Experimental Forest, New Hampshire, U.S.A.     

David I. King  Mariko YamasakiRichard M. DeGraaf  Christine A. Costello 《Forest Ecology and Management》2011,262(1):3-11

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A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples     

Fumi MATSUMOTO  Mariko SHIMA-SAWA  Yu FURUSAWA  Osamu YAMATO  Akira YABUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):832

CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.  相似文献   

Preliminary evaluation of two bathing methods for the management of Malassezia overgrowth in dogs with atopic dermatitis     

Mariko Esumi  Satoko Kanda  Hiromi Shimoura  Yun-Hsiao Hsiao  Keita Iyori 《Veterinary dermatology》2021,32(3):228-e59

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Endocrine mechanisms responsible for different follicular development during the estrous cycle in Hatano high- and low-avoidance rats     

Jaroenporn S  Horii Y  Akieda-Asai S  Wang K  Nagaoka K  Ohta R  Shirota M  Watanabe G  Taya K 《The Journal of reproduction and development》2011,57(6):690-699

Hatano high- and low-avoidance rats (HAA and LAA strains, respectively) were selected and bred according to the avoidance rate in a shuttle-box task. Although they have clear strain differences in ovarian function, their endocrine mechanisms still remain to be clarified. Differences in female reproductive endocrinology between the strains were investigated by means of measuring the plasma concentration of reproductive hormones during the estrous cycle. LAA rats showed approximately threefold lower basal and surge levels of LH, a more than fourfold lower level of FSH surges and higher levels of inhibin A and inhibin B during the estrous cycle compared with the levels seen in HAA rats. The concentration of estradiol-17β in the proestrous stage was significantly lower in LAA rats than in HAA rats. Additionally, LH and FSH secretions from primary cultured anterior pituitary cells with or without in vitro GnRH stimulation were lower in the cells derived from LAA rats and, in terms of FSH secretion, were unresponsive to GnRH in contrast to cells derived from HAA rats. Although an increased number of preantral follicles in diestrus were observed in LAA rats, number of hCG-induced ovulation was lower in LAA rats. LAA rats may have much more follicle growth during the early stage of folliculogenesis, but most follicles might not grow into mature follicles. These results strongly suggest that the strain difference in ovarian function of these two Hatano rats is due to the difference in the regulation of hypothalamo-hypophyseal system for gonadotropins secretion.  相似文献   

Polar cardenolide monoglycosides from stems and twigs of Nerium oleander and their biological activities     

Liming Bai  Ming Zhao  Asami Toki  Toshiaki Hasegawa  Jun-ichi Sakai  Xiao-yang Yang  Yuhua Bai  Hirotsugu Ogura  Tomokazu Mitsui  Takao Kataoka  Mariko Ando  Katsutoshi Hirose  Masayoshi Ando 《Journal of Wood Science》2011,57(1):47-55

Twelve polar cardenolide monoglycosides, 1, 2, 4?C13, and oleagenin (3) were isolated from the methanol extract of stems and twigs of Nerium oleander. Among these, oleagenin (3) and cardenolide monoglycosides named cardenolide B-1 (1) and cardenolide B-2 (2) were isolated from natural sources for the first time. The in vitro antiinflammatory activity of compounds 1?C13 was examined on the basis of inhibitory activity against the induction of the intercellular adhesion molecule-1 (ICAM-1). Compounds 4?C7 were active at an IC₅₀ value of less than 0.4 ??M. The cytotoxic activity of compounds 1?C13 was evaluated against three human cell lines: normal human fibroblast cells (WI-38), malignant tumor cells derived from WI-38 (VA-13), and human liver tumor cells (HepG2). Compounds 4, 6, and 7 were active toward these three cell lines at IC₅₀ values of less than 0.7 ??M, and compounds 5 and 8 were active toward the cell lines at IC₅₀ values of less than 1.5 ??M. The multidrug resistance (MDR) cancer-reversal activity of compounds 1?C13 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780AD cells in the presence of each compound. Compound 1 and 12 showed significant effects on calcein accumulation.  相似文献   

Model studies on volatile release from different semisolid fat blends correlated with changes in sensory perception     

Shiota M  Isogai T  Iwasawa A  Kotera M 《Journal of agricultural and food chemistry》2011,59(9):4904-4912

The effect of dispersed aqueous droplets in water-in-oil (W/O)-emulsion semisolid fats on aroma release and sensory perception was investigated on margarine models where model aroma substances were added. Aroma release from W/O-emulsion fat blends and bulk fat blends with added monoglycerides combining different fatty acids of various short-chain free fatty acids, methylketones, esters, and lactones were measured using headspace solid phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS), and their perception profiles were evaluated by sensory analysis. The presence of aqueous phase in a fat blend significantly reduced the headspace concentrations of butanoic acid and hexanoic acid, and also decreased the perceived intensity of total aroma and cheesy aroma. The aroma release of methylketones, esters, and lactones from the W/O-emulsion fat blends increased with increasing carbon chain length of the volatile molecules. The intensity of aroma perception in a W/O-emulsion fat blend depended on the melting point of the fatty acids (oleic, palmitic, stearic, and behenic) of the monoglyceride used as an emulsifier. Thus, aroma release from a W/O-emulsion semisolid fat blend was influenced by interactions between aroma volatiles and the dispersed aqueous droplets and by their viscoelastic properties.  相似文献