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71.
Mariko KUSE Hwa-Yong LEE Tomas J. ACOSTA Takuo HOJO Kiyoshi OKUDA 《The Journal of reproduction and development》2013,59(4):346-352
Cortisol (Cr), the most important glucocorticoid (GC), is well known to suppress uterine
prostaglandin F2α (PGF) production. However, the details of the regulatory mechanisms
controlling the cyclic changes in endometrial PGF production remain unclear. Here we
investigated the expression of the GC receptor (GC-Rα), the actions of cortisol throughout
the estrous cycle and the regulatory mechanism of GC-Rα in the bovine endometrium. The
levels of GC-Rα protein were greater at the mid-luteal stage (Days 8–12) than at the other
stages. Cr more strongly suppressed PGF production at the mid-luteal stage than at the
follicular stage. GC-Rα expression was increased by progesterone (P4) but decreased by
estradiol-17β (E2) in cultured endometrial stromal cells. The overall results suggest that
ovarian steroid hormones control the cyclic changes in endometrial PGF production by
regulating GC-Rα expression in bovine endometrial stromal cells. 相似文献
72.
Minami W. OKUYAMA Michito SHIMOZURU Go ABE Mariko NAKAI Mariko SASHIKA Ken-Ichiro SHIMADA Nobuhiro TAKAHASHI Daisuke FUKUI Ryohei NAKAMURA Toshio TSUBOTA 《The Journal of reproduction and development》2013,59(4):361-367
The raccoon (Procyon lotor), indigenous to North America, has
naturalized in Japan as an invasive alien species, having been introduced into the country
in the 1970s. In Hokkaido, the northernmost island of Japan, feral raccoons have been
increasing in number and spreading throughout the island. The age at the onset of puberty
for raccoons is important for estimating individual lifetime reproductive success and
population growth. The present study investigated the timing of and potential factors
affecting the onset of puberty in male raccoons in Hokkaido. External characteristics and
histology of testes were studied in 151 male feral raccoons and in 1 captive juvenile. For
the majority of feral yearling raccoons, prepubertal development began in May, and
spermatozoa production began in October prior to their second mating season. However, some
larger juveniles attained puberty during the juvenile period. The captive juvenile, which
was fed throughout the winter, attained puberty only 11 months after birth. These results
suggest that if male raccoons can achieve enough body growth before the first mating
season, puberty can be attained early. In both juveniles and yearlings, spermatozoa
production was only observed after autumn. This timing coincided with the recrudescence of
seasonally active spermatogenesis in adult males. Therefore, attaining puberty in male
raccoons appears to require both adequate body nutrient development and several
environmental factors that control seasonal testicular changes. 相似文献
73.
74.
The objective of this study was to establish a simple and rapid immunocytochemical technique that can be used in veterinary diagnostic cytology. Air-dried impression smears were collected from canine tumors. Samples of epithelial tumors, mesenchymal tumors, and malignant peripheral nerve sheath tumors and melanomas were used for detection of cytokeratin, vimentin, and S-100 protein, respectively. The labeled streptavidin-biotin system was used in the present study. Optimal fixation was determined using standard immunocytochemical procedures, and acetone fixation was found to be the most effective. Optimal concentrations of primary and secondary antibodies were determined at a preset 5-min incubation. Omission of H(2)O(2) treatment, shortening the time for blocking and labeled-streptavidin incubation, and simplifying washing did not decrease immunopositive intensities or enhance false-positive reactions. The described rapid protocol requires approximately 45min without the use of any special equipment. 相似文献
75.
Isoko Takahashi Takanori Sugimoto Yasuo Takasu Mariko Yamasaki Yasutoshi Sasaki Youji Kikata 《Wood Science and Technology》2012,46(1-3):419-429
The thermoplastic flow behavior of cedar flour steamed at different temperatures in the range of 160–220°C was measured using a rheometer and compared with that of beech flour. The temperature at which the cedar flour starts to flow was approximately 70°C higher than that of beech flour, and the cedar flour exhibited low flowability. Furthermore, thermoplastic moldings were prepared from cedar and beech flours, and their physical properties were examined. Similar to the case of beech, a resin-like molding with a density of approximately 1.45?g/cm3 was obtained from the cedar flour steamed at 180°C or higher, and it was revealed by SEM observation that in these moldings wood flour particles adhere to each other. The specific bending strength was maximum for the moldings obtained from 180°C-steamed flour for both types of wood. 相似文献
76.
Mariko Takano Noriko Hayashi Masaya Nakamura Muneyoshi Yamaguchi 《Journal of Wood Science》2009,55(4):302-307
The distribution of an extracellular peroxidase reaction by white-rot fungus Phanerochaete crassa WD1694 was visualized by peroxidase activity staining. The extracellular peroxidase reaction occurred at the hyphal tips
and in the fungal slime filling the gaps between the hyphae. We investigated whether the peroxidase reaction occurred from
the hyphal tips or in the slime. The hyphal tips were observed by phase-contrast microscopy, which showed that slime did not
exist around the hyphal tips. Time-course observation of hyphal tips showed that peroxidase staining became thick and intense
at the tips that did not have fungal slime. Daily observation of the peroxidase staining revealed that the staining was first
observed at the hyphal tips. Furthermore, strongly stained hyphae were observed in the stained slime. These results suggested
that an active species that oxidizes a peroxidase substrate is first produced at the tips of the hyphae, and then occurs in
the slime via diffusion when slime exists around the hyphae. Our results show that the extracellular peroxidase reaction that
is important to lignin biodegradation by white-rot fungi occurs directly at the tips of the hyphae and in the slime.
Part of this report was presented at the 50th Lignin Symposium, October 19–20, 2005, Nagoya, Japan 相似文献
77.
Manli Li Nana Yuyama Mariko Hirata Jianguo Han Yunwen Wang Hongwei Cai 《Euphytica》2009,170(3):327-338
A consensus genetic linkage map with 447 SSR markers was constructed for zoysiagrass (Zoysia japonica Steud.), using 86 F1 individuals from the cross ‘Muroran 2’ × ‘Tawarayama Kita 1’. The consensus map identified 22 linkage groups and had a total
length of 2,009.9 cM, with an average map density of 4.8 cM. When compared with a previous AFLP-SSR linkage map, the SSR markers
from each linkage group mapped to similar positions in both maps. Eight pairs of linkage groups from the AFLP-SSR map were
joined into eight new groups in the current map. This zoysiagrass consensus map contained 35 SSR markers exhibiting high homology
with rice genomic sequences from known chromosomal locations. This allowed synteny to be identified between Zoysiagrass linkage
groups 2, 3, 9, 19 and rice chromosomes 3, 12, 2, 7 respectively. These results provide important comparative genomics information
and the new map is now available for quantitative trait locus analysis, marker-assisted selection and breeding for important
traits in zoysiagrass. 相似文献
78.
Fast‐to‐slow shift of muscle fiber‐type composition by dietary apple polyphenols in rats: Impact of the low‐dose supplementation
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Wataru Mizunoya Shinpei Okamoto Hideo Miyahara Mariko Akahoshi Takahiro Suzuki Mai‐Khoi Q. Do Hideaki Ohtsubo Yusuke Komiya Mulan Qahar Toshiaki Waga Koichi Nakazato Yoshihide Ikeuchi Judy E. Anderson Ryuichi Tatsumi 《Animal Science Journal》2017,88(3):489-499
Our previous studies demonstrated that an 8‐week intake of 5% (w/w) apple polyphenol (APP) in the diet improves muscle endurance of young‐adult rats. In order to identify a lower limit of the dietary contribution of APP to the effect, the experiments were designed for lower‐dose supplementation (8‐week feeding of 0.5% APP in AIN‐93G diet) to 12‐week‐old male Sprague‐Dawley rats. Results clearly showed that the 0.5% APP diet significantly up‐regulates slower myosin‐heavy‐chain (MyHC) isoform ratios (IIx and IIa relative to total MyHC) and myoglobin expression in lower hind‐limb muscles examined (P < 0.05). There was a trend to increased fatigue resistance detected from measurements of relative isometric plantar‐flexion force torque generated by a stimulus train delivered to the tibial nerve (F(98, 1372) = 1.246, P = 0.0574). Importantly, there was no significant difference in the animal body‐phenotypes or locomotor activity shown as total moving distance in light and dark periods. Therefore, the present study encourages the notion that even low APP‐intake may increase the proportions of fatigue‐resistant myofibers, and has promise as a strategy for modifying performance in human sports and improving function in age‐related muscle atrophy. 相似文献
79.
A method of determining the Young’s modulus of timber using the stress wave propagation velocity without knowing the timber
density was developed in our previous study. This method enables the estimation of Young’s modulus by Monte Carlo simulation
using an existing database of the Young’s modulus versus density relationship as reference. Here, in Part II, we consider
the effect of the reference distribution database on the accuracy of the estimated Young’s modulus by the developed method.
Twelve different reference distribution databases were used in this study, containing Young’s modulus versus density data
for more than 13 000 real-size timber specimens of ten different species. We obtained the following results: (1) the distribution
of Young’s modulus estimated using an arbitrary stress wave propagation velocity depends on the reference distribution database
employed, (2) the most important factor is not that the reference database has data on the same species as the timber in the
test, but rather that the reference distribution database covers the foreseeable range of timber densities within the test,
and (3) the estimation accuracy is higher than about 80% when the database covers many species and has wide ranges of densities
and Young’s moduli. This estimation method was developed in order to measure the Young’s modulus of timber whose density cannot
be measured. Considering that the quality of lumber has a large variation, such estimation accuracy will be useful for practical
applications. 相似文献
80.
Peroxidase activity staining localized at hyphal tips of white-rot fungus Phanerochaete crassa WD1694 that was cultivated in a shaken liquid culture containing unbleached kraft pulp was investigated. Manganese peroxidase was detected in culture solution, washing solution of mycelium, and mycelial extract. Glyoxal oxidase was detected only in mycelial extract and was not detected in culture solution. Addition of hydrogen peroxide generated peroxidase activity staining in the culture solution. Addition of catalase resulted in no staining in the culture of P. crassa WD1694, and the addition of methylglyoxal resulted in marked peroxidase activity staining at hyphal tips and on hyphal wall. In an optimized culture, glyoxal oxidase was produced in culture solution. Although the production of glyoxal oxidase and manganese peroxidase had a positive correlation, the secretion and the peak of glyoxal oxidase was observed 3 and 2 days later than those of manganese peroxidase. The N-terminal sequence of purified glyoxal oxidase had very high homology with that of P. chrysosporium. These results elucidated the hydrogen peroxide supply system in lignin biodegradation by white-rot fungi, i.e., while remaining on the hyphal cell wall, glyoxal oxidase provides hydrogen peroxide to manganese peroxidase that had diffused into the culture solution beforehand. 相似文献