Human focal nodular hyperplasia-like lesion in the liver of a cat     

K Ochiai  M Takechi  T Matsumoto  C Itakura 《The Japanese journal of veterinary research》1990,38(3-4):117-125

A young cat clinically displaying marked ascites had multiple nodule hyperplasia in the liver. One of the nodules sampled at exploratory laparotomy was studied histopathologically. The nodule consisted mainly of hyperplastic hepatocytes with a central stellate scarlike connective tissue. The connective tissue had thick-walled blood vessels and radiating bands of fibrous connective tissue extending peripherally, dividing the nodule into pseudolobules. The lesions resembled focal nodular hyperplasia (FNH) in man. Serial section revealed a single large artery with the splitting of the internal elastic lamina running into the central connective tissue and branching to connect with the main vessels. The arterial branchings formed a spider-like structure. From these findings, it was suggested that the nodule had developed from a hepatic response to pre-existing vascular anomalies as had been reported in human FNH.  相似文献   

Acute respiratory failure caused by leukaemic infiltration of the lung of a dog     

Mori T  Kadosawa T  Okada Y  Ochiai K  Umemura T  Tanoue S  Okumura M  Fujinaga T 《The Journal of small animal practice》2001,42(7):349-351

A seven-year-old crossbred male dog with a suspected leukaemic condition was referred for investigation and treatment. A bone marrow aspirate revealed an acute myeloid leukaemia. Combination chemotherapy was administered and the dog initially improved, but 18 days after the initiation of therapy its body condition deteriorated and the animal developed acute respiratory distress. On postmortem examination, extensive leukaemic pulmonary infiltrates were evident.  相似文献   

Neospora caninum infected the alimentary tract of nude mice and was transmitted to other mice by intraperitoneal inoculation with the intestinal contents     

Park CH  Sawada M  Morita T  Shimada A  Ochiai K  Umemura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(5):525-527

Neospora caninum (BT-2 strain) that originated from the brain of a Holstein calf was serially passaged through 10 generations of BALB/c nude mice by intraperitoneal inoculation. Histological examination of the mice revealed that numerous clusters of tachyzoites appeared in the pancreas, stomach and small intestine as well as in the central nervous system (CNS) and skeletal muscles. Intestinal contents of the infected mice were inoculated intraperitoneally into uninfected nude mice and 3 of the 17 inoculated mice showed clinical signs at post inoculation days 3 to 10. The present experiments demonstrated a proliferation of N. caninum tachyzoites in the mucosa of the alimentary tract and pancreas of the nude mice and the intestinal contents of the mice were infective to other nude mice.  相似文献   

Intestinal candidiasis in a dog     

Ochiai K  Valentine BA  Altschul M 《The Veterinary record》2000,146(8):228-229

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Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats     

Yu FURUSAWA  Masashi TAKAHASHI  Mariko SHIMA-SAWA  Hitoshi HATAI  Noriaki MIYOSHI  Osamu YAMATO  Akira YABUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1363

Epithelial–mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats (n=9) were stained using mouse monoclonal anti-E-cadherin and rabbit monoclonal anti‐vimentin antibodies. Enzymatic immunohistochemistry (IHC) revealed that 51.6% (8/22 in dogs, 8/9 in cats) of the cases showed EMT. In dogs, FICC could detect EMT in 62.5% (5/8) of those cases. In cats, FICC could detect EMT in 100% (8/8) of the cases. In conclusion, the present FICC method could successfully detect EMT using conventional air-dried cytology smear slides.  相似文献   

Corneal-Protective Effects of an Artificial Tear Containing Sodium Hyaluronate and Castor Oil on a Porcine Short-Term Dry Eye Model     

Takashi HASEGAWA  Hideki AMAKO  Takeshi YAMAMOTO  Mariko TAZAWA  Yuji SAKAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1219-1224

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Establishment and characterization of immortalized bovine endometrial epithelial cells     

Hanako Bai  Toshihiro Sakurai  Rulan Bai  Sachiko Yamakoshi  Etsunari Aoki  Mariko Kuse  Kiyoshi Okuda  Kazuhiko Imakawa 《Animal Science Journal》2014,85(8):799-804

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Highly regioselective amination of unactivated alkanes by hypervalent sulfonylimino-λ³-bromane     

Ochiai M  Miyamoto K  Kaneaki T  Hayashi S  Nakanishi W 《Science (New York, N.Y.)》2011,332(6028):448-451

Amination of alkanes has generally required metal catalysts and/or high temperatures. Here we report that simple exposure of a broad range of alkanes to N-triflylimino-λ(3)-bromane 1 at ambient temperature results in C-H insertion of the nitrogen functionality to afford triflyl-substituted amines in moderate to high yields. Marked selectivity for tertiary over secondary C-H bonds was observed; primary (methyl) C-H bonds were inert. Addition of hexafluoroisopropanol to inhibit decomposition of 1 dramatically improved the C-H amination efficiencies. Second-order kinetics, activation parameters (negative activation entropy), deuterium isotope effects, and theoretical calculations suggest a concerted asynchronous bimolecular transition state for the metal-free C-H amination event.  相似文献   

COVER IMAGE     

Hiroyuki Tani  Sena Kurita  Ryo Miyamoto  Kazuhiko Ochiai  Kyoichi Tamura  Makoto Bonkobara 《Veterinary and comparative oncology》2020,18(2)

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Characterization of fast skeletal myosin from white croaker in comparison with that from walleye pollack     

Yoshie Satoh  Misako Nakaya  Yoshihiro Ochiai  Shugo Watabe 《Fisheries Science》2006,72(3):646-655

ABSTRACT:   Enzymatic and structural properties of white croaker fast skeletal muscle myosin were determined and compared with those of walleye pollack counterpart. Ca²⁺-ATPase activity of white croaker myosin was decreased to approximately 70% of the original activity during 1 day of storage at 0°C and pH 7.0 in 0.5 M KCl and 0.1 mM dithiothreitol, whereas that of walleye pollack was decreased to approximately 20% under the same condition. The activation energy ( E _a) for inactivation of white croaker myosin calculated by the Arrhenius plot for inactivation rate constant (K_D) was 1.2-fold higher than that of walleye pollack. While Ca²⁺-ATPase showed a similar KCl-dependency for the two species, the maximal activity was observed at pH 6.2 and 6.3 for white croaker and walleye pollack, respectively. Actin-activated myosin Mg²⁺-ATPase activity of white croaker was approximately half that of walleye pollack at 0.05 M KCl and pH 7.0, although the two myosins showed a similar affinity to F-actin with K _m of 1.7 and 1.4, respectively. Limited proteolysis with α-chymotrypsin cleaved heat-denatured white croaker myosin mainly at heavy meromyosin/light meromyosin (HMM/LMM) junction, whereas walleye pollack myosin was cleaved at several sites in LMM as well as at the HMM/LMM junction.  相似文献