Accumulation mechanism of γ-aminobutyric acid in tomatoes (Solanum lycopersicum L.) under low O2 with and without CO2     

Mae N  Makino Y  Oshita S  Kawagoe Y  Tanaka A  Aoki K  Kurabayashi A  Akihiro T  Akama K  Koike S  Takayama M  Matsukura C  Ezura H 《Journal of agricultural and food chemistry》2012,60(4):1013-1019

The storage of ripe tomatoes in low-O(2) conditions with and without CO(2) promotes γ-aminobutyric acid (GABA) accumulation. The activities of glutamate decarboxylase (GAD) and α-ketoglutarate-dependent GABA transaminase (GABA-TK) were higher and lower, respectively, following storage under hypoxic (2.4 or 3.5% O(2)) or adjusted aerobic (11% O(2)) conditions compared to the activities in air for 7 days at 25 °C. GAD activity was consistent with the expression level of mRNA for GAD. The GABA concentration in tomatoes stored under hypoxic conditions and adjusted aerobic conditions was 60-90% higher than that when they are stored in air on the same day. These results demonstrate that upregulation of GAD activity and downregulation of GABA-TK activity cause GABA accumulation in tomatoes stored under low-O(2) conditions. Meanwhile, the effect of CO(2) on GABA accumulation is probably minimal.  相似文献   

Evaluation of antixenosis in soybean against Spodoptera litura by dual-choice assay aided by a statistical analysis model: Discovery of a novel antixenosis in Peking     

Mariko Yano  Takato Inoue  Ryu Nakata  Masayoshi Teraishi  Naoko Yoshinaga  Hajime Ono  Yutaka Okumoto  Naoki Mori 《Journal of Pesticide Science》2021,46(2):182

The method for evaluating soybean (Glycine max) antixenosis against the common cutworm (Spodoptera litura) was developed based on a dual-choice assay aided by a statistical analysis model. This model was constructed from the results of a dual-choice assay in which Enrei, a soybean cultivar susceptible to S. litura, was used as both a standard and a test leaf disc for 2nd–5th instar larvae. The statistical criterion created by this model enabled the evaluation of the presence of antixenosis. This method was applied to four soybean varieties, including Tamahomare (susceptible), Himeshirazu (resistant), IAC100 (resistant), and Peking (unknown), as well as Enrei. Subsequently, the degrees of antixenosis were also compared by F-test, followed by maximum likelihood estimation (MLE). According to the results, the antixenosis of Tamahomare, Himeshirazu, and IAC100 was statistically reevaluated and Peking exhibited a novel antixenosis, which was stronger for 3rd–5th instar larvae than for 2nd instar.  相似文献   

Effect of chemical fertilizer and manure application on N₂O emission from reed canary grassland in Hokkaido, Japan     

Tao JIN  Mariko SHIMIZU  Satoru MARUTANI  Alexey R. DESYATKIN  Natsumi IIZUKA  Hiroshi HATA  Ryusuke HATANO 《Soil Science and Plant Nutrition》2010,56(1):53-65

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Genetic variation affects de novo translocation frequency     

Kato T  Inagaki H  Yamada K  Kogo H  Ohye T  Kowa H  Nagaoka K  Taniguchi M  Emanuel BS  Kurahashi H 《Science (New York, N.Y.)》2006,311(5763):971

Translocation is one of the most frequently occurring human chromosomal aberrations. The constitutional t(11;22)(q23;q11), which is the only known recurrent non-Robertsonian translocation, represents a good model for studying translocations in humans. Here we demonstrate polymorphisms of the palindromic sequence at the t(11;22) breakpoint that affect the frequency of de novo translocations in sperm from normal males. A typical allele consists of a perfect palindrome, producing ~10-5 de novo t(11;22) translocations. Alleles with an asymmetric center do not form the t(11;22). Our data show the importance of genome sequence on chromosomal rearrangements, a class of human mutation that is thought to be random.  相似文献   

An insertion/deletion variant of a thymine base in exon 2 of the porcine beta 3‐adrenergic receptor gene associated with loin eye muscle area     

Kensuke HIROSE  Mariko NAKAMURA  Tatsuya TAKIZAWA  Kazuo FUKAWA  Tetsuya ITO  Masayuki UEDA  Takashi SASAKI  Kazuaki TANAKA 《Animal Science Journal》2009,80(6):624-630

An insertion/deletion variant of a thymine base (T5 and T6) in exon 2 of porcine beta 3‐adrenergic receptor (ADRB3) gene has been described. In the current study, we made an association study between the ADRB3 polymorphisms and production traits in 735 Duroc pigs. The allele frequencies for the T5 and T6 alleles in our study population were 0.433 and 0.567, respectively. Any associations between ADRB3 genotype and average daily weight gain during test period, or backfat thickness and intramuscular fat content were not detected in either sex. However the size of the loin eye muscle area (EMA) was significantly associated with ADRB3 genotypes in gilts. T6‐homozygous gilts had a higher mean of EMA (40.6 ± 0.6 cm²) than T5‐homozygous (38.1 ± 0.4 cm², P = 0.002) and heterozygous (38.8 ± 0.3 cm², P = 0.034) gilts. This association was not detected in males. In addition, a multiple traits animal model best linear unbiased predictor (BLUP) analysis revealed that the T6‐homozygous genotype had positive effects on breeding value of EMA. Accordingly, we suggest that ADRB3 polymorphism has the potential to be an important genetic marker for prediction of EMA in Duroc pigs.  相似文献   

Recombinant expression and characterization of three group IB phospholipase A₂ isoforms in the red sea bream Chrysophrys major     

Yukichi  FUJIKAWA  Mariko  SHIMOKAWA  Shoko  WATANABE  Akiko  TAKEHARA  Satoshi  UCHIYAMA  Seiichi  HAYASHI  Muneharu  ESAKA  Hiroshi  MIYAMOTO  Mitsumasa  MANKURA  Noriaki  IIJIMA 《Fisheries Science》2003,69(6):1263-1270

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小分子热激蛋白基因在番茄花药中的转录   总被引：7，自引：0，他引：7  

刘箭  庄野真理子 《园艺学报》2001,28(5):403-406

 选择8 个耐热性不同的番茄品种种植在地处亚热带的日本最南部石垣市, 测定夏季高温环境下花粉粒的败育率, 并据此将其分成耐热型和热敏型品种, 分析高温胁迫下花药中4 类小分子热激蛋白的转录。结果表明, 番茄花药中含有组成性的细胞质小分子热激蛋白和线粒体小分子热激蛋白mRNA; 高温季节细胞质小分子热激蛋白和线粒体小分子热激蛋白基因转录明显; 热敏型番茄花药中小分子热激蛋白基因的转录高峰温度低于耐热型花药。  相似文献   

Technological development for the control of humidity conditioning performance of slit materials made from Japanese cedar     

Yuka Miyoshi  Mariko Furutani  Mayuko Ishihara  Shunichi Tai  Yuzo Furuta  Shuichi Kawai 《Journal of Wood Science》2015,61(6):641-646

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Three decades of avian research on the Bartlett Experimental Forest, New Hampshire, U.S.A.     

David I. King  Mariko YamasakiRichard M. DeGraaf  Christine A. Costello 《Forest Ecology and Management》2011,262(1):3-11

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A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples     

Fumi MATSUMOTO  Mariko SHIMA-SAWA  Yu FURUSAWA  Osamu YAMATO  Akira YABUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):832

CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.  相似文献