CD4+CD25+ T Cells in Maedi Visna Infection: Preliminary Immunohistochemical Study in Experimentally Infected Sheep     

Mariotti  F.  Preziuso  S.  Rossi  G.  Taccini  E.  Braca  G.  Renzoni  G. 《Veterinary research communications》2007,31(1):237-239

Veterinary Research Communications - Mariotti, F., Preziuso, S., Rossi, G., Taccini, E., Braca, G. and Renzoni, G., 2007. CD4+CD25+ T cells in Maedi Visna infection: preliminary immunohistochemical...  相似文献   

Genotyping and evaluation of local olive varieties of a climatically disfavoured region through molecular,morphological and oil quality parameters     

Annalisa Rotondi  Nicolò G.M. Cultrera  Roberto Mariotti  Luciana Baldoni 《Scientia Horticulturae》2011

The evaluation criteria applied on a 34 set of minor and main olive varieties of a restricted and climatically disfavoured region of Italy have allowed to clearly identify some local varieties with valuable agro-ecological adaptation and outstanding oil quality traits. These traits are necessary for the establishment and/or improvement of olive culture in a disadvantaged cultivation area. The molecular and morphological analyses have allowed to establish the identity of local unknown ecotypes, establishing their relationships either with other local varieties or with wide-spread cultivars, while the evaluation of oil quality parameters ranked their suitability for further exploitation.  相似文献   

Assessment of postoperative pain after unilateral mastectomy using two different surgical techniques in dogs     

Bruno W Minto  Lisiane C Rodrigues  Paulo VM Steagall  Eduardo R Monteiro  Claudia VS Brand?o 《Acta veterinaria Scandinavica》2013,55(1):60

Background

There are few studies reporting pain and postoperative analgesia associated with mastectomy in dogs. The aim of this study was to evaluate postoperative pain after unilateral mastectomy using two different surgical techniques in the dog.

Findings

Twenty female dogs were assigned (n=10/group) to undergo unilateral mastectomy using either the combination of sharp and blunt dissection (SBD) or the modified SBD (mSBD) technique, in which the mammary chain is separated from the abdominal wall entirely by blunt (hand and finger) dissection except for a small area cranial to the first gland, in a prospective, randomized, clinical trial. All dogs were premedicated with intramuscular acepromazine (0.05 mg/kg) and morphine (0.3 mg/kg). Anesthesia was induced with intravenous ketamine (5 mg/kg) and diazepam (0.25 mg/kg), and maintained with isoflurane. Subcutaneous meloxicam (0.2 mg/kg) was administered before surgery. Postoperative pain was evaluated according to the University of Melbourne pain scale (UMPS) by an observer who was blinded to the surgical technique.. Rescue analgesia was provided by the administration of intramuscular morphine (0.5 mg/kg) if pain scores were >14 according to the UMPS. Data were analyzed using t-tests and ANOVA (P>0.05). There were no significant differences between the groups for age, weight, extubation time, and duration of surgery and anesthesia (P>0.05). There were no significant differences for postoperative pain scores between groups. Rescue analgesia was required in one dog in each group.

Conclusions

The two surgical techniques produced similar surgical times, incidence of perioperative complications and postoperative pain. Multimodal analgesia is recommended for treatment of postoperative pain in dogs undergoing unilateral mastectomy.  相似文献   

Clonal propagation in the genus Ulmus: Improvement of rooting ability by Agrobacterium rhizogenes T-DNA genes     

C. Rinallo  L. Mittempergher  G. Frugis  D. Mariotti 《The Journal of Horticultural Science and Biotechnology》2013,88(4):502-506

Summary

Elm clones may be propagated by grafting, but graft incompatibility is a major problem. Rooting by cuttings does not always give satisfactory results. Shoots of elm clones resistant to Dutch Elm Disease (caused by Ophiostoma ulmi Buism.) and with poor rooting ability, were air layered after one of the following five treatments: application of growth regulator mixture; inoculation with Agrobacterium rhizogenes wild type 1855 (A.r. 1855) or Agrobacterium tumefaciens-ORF11 (A.t. ORF11); application of growth regulator mixture and inoculation with A.r. 1855 or A.t. ORF11. Shoots treated only with growth regulators exhibited low percentage of rooting; shoots inoculated with only Agrobacterium strains produced very low percentage of rooting; shoots treated with growth regulator and inoculated with Agrobacterium strains exhibited the best percentage of rooting. The results demonstrated the possibility of rooting of these recalcitrant clones, with clear advantage for the propagation of elm clones with growth regulator and A.t. ORF11.  相似文献   

Pain-related aggression in dogs: 12 clinical cases     

Tomás Camps  Marta Amat  Valentina M. Mariotti  Susana Le Brech  Xavier Manteca 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(2):99-102

The aim of this retrospective study was to describe the main features of pain-related aggression in dogs. Twelve dogs presented for aggressive problems at the Veterinary Hospital of the Autonomous University of Barcelona, Spain, were included, and a questionnaire was used to gather information on the context of the aggression, body posture during the attack, impulsiveness, and aggressive behavior before the onset of the pain-eliciting condition. The most common cause of pain was hip dysplasia (66.7%), but no relationship was found between the cause of pain and the characteristics of the aggressive behavior. Dogs were classified as having been aggressive before or after the onset of painful condition. Dogs that had not been aggressive before the onset of the pain-eliciting condition were more impulsive (df = 1, χ² = 5.3, P = 0.0209), showed aggression as a result of manipulation context more frequently (df = 1, χ² = 6, P = 0.0143), and adopted a defensive body posture more frequently (df = 1, χ² = 3.733, P = 0.0533) than dogs that had been aggressive before the onset of pain. These results suggest that previous expression of aggressive behavior has a major effect on the pattern of pain-related aggression in dogs.  相似文献   

The abundance of natural nitrogen 15 in the organic matter of soils along an altitudinal gradient (Chablais,Haute Savoie,France)     

A. Mariotti  D. Pierre  J.C. Vedy  S. Bruckert  J. Guillemot 《CATENA》1980,7(1):293-300

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Enzootic ulcer in the back of the tongue in cattle after ingestion of hay containing flower clusters of yellow bristle-grass     

Fava E  Rossi F  Speranzini G  Nigrelli A  Rossignoli G  Gelmetti D  Mariotti MG  Sali G  Stöber M  Wolf P  Von Boberfeld O 《DTW. Deutsche tier?rztliche Wochenschrift》2000,107(9):351-354

Chronically recidivating enzootic ulcerations in the tongues of numerous milking cows in the Po river plain area in Italy. The animals were permanently kept indoors in cubicle houses and fed by hay containing high amounts of ripe yellow bristle grass (Setaria glauca (L.) Beauv. [= S. pumila Poiret]). The panicled parts of the culms were found to be the cause of the massive injuries.  相似文献   

Tree shelters affect shoot and root system growth and structure in Quercus robur during regeneration establishment     

Barbara Mariotti  Alberto Maltoni  Douglass F. Jacobs  Andrea Tani 《European Journal of Forest Research》2015,134(4):641-652

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Aerophobin-1 from the Marine Sponge Aplysina aerophoba Modulates Osteogenesis in Zebrafish Larvae     

Marta Carnovali  Maria Letizia Ciavatta  Ernesto Mollo  Vassilios Roussis  Giuseppe Banfi  Marianna Carbone  Massimo Mariotti 《Marine drugs》2022,20(2)

Longer life expectancy has led to an increase in efforts directed to the discovery of new healing agents for disorders related to aging, such as bone diseases. Harboring an incredible variety of bioactive metabolites, marine organisms are standing out as fruitful sources also in this therapeutic field. On the other hand, the in vivo zebrafish model has proven to be an excellent low-cost screening platform for the fast identification of molecules able to regulate bone development. By using zebrafish larvae as a mineralization model, we have thus evaluated the effects of the crude acetonic extract from the marine sponge Aplysina aerophoba and its bromotyrosine components on bone development. Obtained results led to the selection of aerophobin-1 (1) as a promising candidate for applications in regenerative medicine, paving the way for the development of a novel therapeutic option in osteoporosis treatment.  相似文献   

Dynamics of soil microbial populations involved in 2,4-D biodegradation revealed by FAME-based Stable Isotope Probing     

Thomas Z. Lerch  Marie-France Dignac  Gérard Bardoux  André Mariotti 《Soil biology & biochemistry》2009,41(1):77-85

Stable Isotope Probing (SIP) is a powerful tool for analysing the fate of pesticides in soil. Together with FAME (Fatty Acid Methyl Esters), it can help identify biodegradation pathways and recycling into the microbial biomass. The fate of ring-labelled ¹³C-2,4-dichlorophenoxyacetic acid or 2,4-D (C_2,4-D) was determined in soil during a 6-month incubation. The distribution of ¹³C among the microbial biomass, the CO₂ respired, the water, methanol and dichloromethane soluble fractions, and the residual non-extracted bulk soil was measured. Molecular analyses were carried out on the lipid and the non-extractable fractions. After 8 days, about half of the initial amount of C_2,4-D was mineralised; the other half remained in soil as non-extractable residues (NER). C_2,4-D continued to be mineralised, suggesting that NER were still bioavailable. Analysis of C_2,4-D-enriched FAME contained in the lipid fraction suggested that a succession of microbial populations was involved in 2,4-D biodegradation. This is possibly due to the change of 2,4-D availability. The C_2,4-D yield coefficient and degrader diversity evolved during the incubation, providing corroboratory evidence that different physiological groups were active during the incubation. The ¹³C-labelled microbial community was always less diverse than the total community, even at the end of the incubation, suggesting that the cross-feeding community is also a specific part of the total community. This work shows that molecular analysis of ¹³C-labelled pesticides is a useful tool for understanding both chemical and biological aspects of their fate in soil.  相似文献