Cytological analysis of bronchoalveolar lavage fluid acquired by bronchoscopy in healthy ferrets: A pilot study     

Marjorie Bercier  Isabelle Langlois  Marilyn Dunn  Pierre Hélie  Patrick Burns  Carolyn Gara-Boivin 《Canadian journal of veterinary research》2016,80(1):74-80

The objective of this study was to investigate the normal cytological evaluation of bronchoalveolar lavage (BAL) fluid in healthy adult ferrets (N = 12). These ferrets underwent bronchoscopy and BAL using sterile saline [1.5 mL/kg body weight (BW)]. Percentage of fluid recovered, total leukocyte count, differential leukocyte count, and cell count of the epithelial lining fluid (ELF) were determined. The mean percentage of lavage volume recovered from the right lung and left lung were 67.8 ± 14.9% and 69.7 ± 20.0%, respectively. Gender (P = 0.12) and weight (P = 0.17) did not significantly affect the mean percentage of recovered volume. The mean percentage of recovered volume (P = 0.47) and the mean leukocyte count (P = 0.17) from the right and left lung were not significantly different. Macrophages were the main leukocyte component of the lavages, followed by neutrophils, lymphocytes, and eosinophils. The mean proportion of ELF in BAL fluid was 9.3 ± 3.7% v/v. Bronchoscopy is clinically useful for collecting good quality BAL samples for cytological analysis in ferrets. The leucocyte differential was established, which may help veterinarians to make better clinical decisions when treating respiratory disease. Further studies are required with a larger group in order to establish the healthy reference intervals for BAL values in ferrets.  相似文献   

EPA reduces penalty against biotech firm     

Sun M 《Science (New York, N.Y.)》1986,232(4757):1495

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Carbon balance of afforested peatland in Scotland   总被引：3，自引：0，他引：3  

Hargreaves  K.J.; Milne  R.; Cannell  M.G.R. 《Forestry》2003,76(3):299-317

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Characterisation and cardiac directed differentiation of canine adult cardiac stem cells     

Hodgkiss-Geere HM  Argyle DJ  Corcoran BM  Whitelaw B  Milne E  Bennett D  Argyle SA 《Veterinary journal (London, England : 1997)》2012,192(2):176-182

A multicentre study of 285 cases was performed to enhance the management of distal phalangeal fractures on the basis of clinical evidence. The outcome after treatment was available for 223 of the cases. Horses with a non-articular type I fracture had a better prognosis (91.7%) for return to original or expected level of use than horses with an articular type II or III fracture (69.6% and 74.1%, respectively). The prognosis for types IV and V fractures was fair (57.7% and 57.1%, respectively) and for type VI good (80%). Horses with a hindlimb fracture had a significantly greater chance of a successful outcome. No significant association between age or time to start treatment and success rate was noted. The best treatment option for types I-III fractures was a conservative approach (box rest). Type IV fractures were best treated by arthroscopic removal of the fragment. Immobilisation of the hoof did not seem to influence outcome. Radiological findings and clinical healing were not accurately correlated and the re-commencement of training should be based on clinical rather than radiological findings. Complete osseous union of the fracture was not essential for a successful return to athletic activity.  相似文献   

Effects of maternal nutrition and stage of gestation on body weight, visceral organ mass, and indices of jejunal cellularity, proliferation, and vascularity in pregnant ewe lambs     

Caton JS  Reed JJ  Aitken RP  Milne JS  Borowicz PP  Reynolds LP  Redmer DA  Wallace JM 《Journal of animal science》2009,87(1):222-235

Peripubertal ewe lambs (44.3 +/- 1.1 kg of initial BW) were used in a 2 x 3 factorial design to test the effects of plane of nutrition (diet) and stage of gestation on maternal visceral tissue mass, intestinal cellularity, crypt cell proliferation, and jejunal mucosal vascularity. Singleton pregnancies to a single sire were established by embryo transfer, and thereafter ewes were offered a control (Control) or high (High) amount of a complete diet (2.84 Mcal/kg and 15.9% CP; DM basis) to promote slow or rapid maternal growth rates. After d 90 of gestation, feed intake of the Control group was adjusted weekly to maintain BCS and meet the increasing nutrient demands of the gravid uterus. Ewes were slaughtered at 50 d (n = 6 Control; n = 5 High), 90 d (n = 8 Control; n = 6 High), or 130 d (n = 8 Control; n = 6 High) of gestation. Ewes were eviscerated and masses of individual organs were recorded. The jejunum was sampled and processed for subsequent analyses. Final ewe BW for Control-fed ewes was similar at d 50 and 90 and increased (P = 0.10) from d 90 to 130 (46.0, 48.9, and 58.2 +/- 1.6 kg, respectively), whereas final BW increased (P 相似文献   

Synthesis,mechanism of action,and QSAR of herbicidal 3-substituted-2-aryl-4,5,6,7-tetrahydroindazoles     

John W. Lyga  Russell M. Patera  Marjorie J. Plummer  Blaik P. Halling  Debra A. Yuhas 《Pest management science》1994,42(1):29-36

A factorially designed set of 3-substituted 2-aryl-4,5,6,7-tetrahydroindazoles was synthesized and their herbicidal properties were evaluated using regression analysis. For optimal activity, the substituent at position 3 should be neither strongly hydrophobic nor hydrophilic and should have a small minimum radius. A study of the mechanism of action indicated that the tetrahydroindazoles are inhibitors of the enzyme protoporphyrinogen oxidase.  相似文献   

Phosphate enema toxicosis in a pygmy goat wether          下载免费PDF全文

Hickman SA  Gill MS  Marks SL  Smith JA  Sod GA 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(10):849-851

Phosphate enema toxicity was diagnosed in a 7-month-old, castrated male, pygmy goat. On presentation, clinical findings included mild depression, tachycardia, tachypnea, rumen stasis, muscle tremors, hypocalcemia, hypokalemia, hypochloremia, hyperphosphatemia, azotemia, and metabolic acidosis. Fluid diuresis and parenteral antimicrobial therapy resulted in recovery after 3 d of treatment.  相似文献   

Crushed tail head syndrome in cattle     

Milne M 《The Veterinary record》2003,152(21):666-667

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Purification and serology of virions of impatiens necrotic spot tospovirus     

P. Roggero  G. Adam  R. G. Milne  V. Lisa 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(6):563-568

Impatiens necrotic spot tospovirus (INSV) virions were purified using a procedure devised for tomato spotted wilt tospovirus (TSWV) from systemically infectedNicotiana benthamiana plants grown at 33 °C day/26 °C night and a photoperiod of 14 hours. With plants grown at 24/18 ° C purification was unsuccessful. In SDS-PAGE the protein pattern of INSV was similar to that reported for TSWV, except the appearance of a single G2 protein band. A polyclonal antiserum, prepared against virions, reacted in Western blots with INSV nucleoprotein and glycoproteins but only with TSWV glycoproteins. In DAS ELISA the antiserum reacted with both INSV and TSWV infected plant sap and, after absorption with TSWV, only with INSV. In TAS ELISA the antiserum trapped both INSV and TSWV nucleoproteins and glycoproteins as detected by specific monoclonal antibodies, and, after absorption with TSWV, only the homologous proteins. This appears to be the first report of the purification of INSV virions and the production of an antiserum reacting with both nucleoprotein and glycoprotein antigens.  相似文献   

Barred researcher publishes a paper     

Sun M 《Science (New York, N.Y.)》1983,219(4582):270

Marc J. Straus, barred from receiving federal research grants after admitting that some of the data in a study he headed were fabricated, is the subject of a new controversy. In dispute are a paper by Straus in the Oct 1982 issue of Cancer Treatment Reports, a journal funded by the National Cancer Institute, and the journal's plan to alert its readers to doubts about the validity of the data reported. Questioned by Congressman L.H. Fountain, the journal's editorial board conceded it had failed to ensure that Straus had complied fully with the board's condition that he provide verification of his data by an independent source.  相似文献