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Mycoplasma species have a global distribution causing serious diseases in cattle worldwide including mastitis, arthritis, pneumonia, otitis media and reproductive disorders. Mycoplasma species are typically highly contagious, are capable of causing severe disease, and are difficult infections to resolve requiring rapid and accurate diagnosis to prevent and control disease outbreaks. This review discusses the development and use of different diagnostic methods to identify Mycoplasma species relevant to cattle, with a particular focus on Mycoplasma bovis. Traditionally, the identification and diagnosis of mycoplasma has been performed via microbial culture. More recently, the use of polymerase chain reaction to detect Mycoplasma species from various bovine samples has increased. Polymerase chain reaction has a higher efficiency, specificity, and sensitivity for laboratory diagnosis when compared with conventional culture‐based methods. Several tools are now available for typing Mycoplasma spp. isolates, allowing for genetic characterization in disease outbreak investigations. Serological diagnosis through the use of indirect ELISA allows the detection of antimycoplasma antibodies in sera and milk, with their use demonstrated on individual animal samples as well as BTM samples. While each testing method has strengths and limitations, their combined use provides complementary information, which when interpreted in conjunction with clinical signs and herd history, facilitates pathogen detection, and characterization of the disease status of cattle populations.  相似文献   
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This article examined relationships between proximity to chronic wasting disease (CWD) and perceived risk and trust. The sample included 1,606 hunters in one of 10 northern Illinois counties with CWD, 1,958 hunters in a non-CWD county adjacent to these counties with CWD, and 2,099 hunters from the remaining non-CWD counties in Illinois. Compared to hunters in non-CWD counties, those in CWD counties were hypothesized to: (a) perceive more risk of CWD to humans, (b) perceive more risk of CWD to deer, (c) report less trust in the Illinois Department of Natural Resources (IDNR) CWD information, and (d) have less trust in the IDNR management of CWD. The first two hypotheses were not supported, as hunters in CWD counties perceived less risk to humans and deer than did respondents in non-CWD counties. Hunters in CWD counties, however, were less trusting of the IDNR information and management compared to the other hunters.  相似文献   
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