Development of a Multiplex PCR for the Identification of Major Pathogenic Bacteria of Post‐Partum Endometritis in Dairy Cows     

SM Aghamiri  M Haghkhah  MR Ahmadi  HR Gheisari 《Reproduction in domestic animals》2014,49(2):233-238

Post‐partum period has an important role in cows' breeding due to its effects on reproductive efficiency and subsequent pregnancy. Escherichia coli, Trueperella pyogenes (Arcanobacterium pyogenes), Fusobacterium necrophorum and Prevotella melaninogenicus are recognized as major pathogens associated with uterine endometrial lesions. The objective of this study was to identify these pathogens using the polymerase chain reaction (PCR) as a culture‐independent sensitive method. A total of 172 cows were examined 25–35 days post‐partum, and 128 cows were examined at 2 weeks later (39–49 days post‐partum). Uterine discharges were collected by covered plastic infusion pipettes. The prevalence of endometritis was greater in the first examination than the second (35.5% vs. 16%). E. coli was detected in eight of the samples, T. pyogenes was detected in 13 of the samples and F. necrophorum was detected in 11 of the samples. There was no positive sample of P. melaninogenicus. Uterine contamination by T. pyogenes and F. necrophorum in the first examination was higher than the second examination. T. pyogenes affected as a tendency the prevalence of clinical endometritis in first examination. Primiparous cows showed 4.02 times higher odds of clinical endometritis compared with second‐parity cows in first examination. A multiplex PCR protocol as a simple, less expensive, fast assay was introduced to identify E. coli, T. pyogenes and F. necrophorum.  相似文献   

Combined preputial advancement and phallopexy as a revision technique for treating paraphimosis in a dog     

SM Wasik  AM Wallace 《Australian veterinary journal》2014,92(11):433-436

A 7‐year‐old neutered male Jack Russell terrier‐cross was presented for signs of recurrent paraphimosis, despite previous surgical enlargement of the preputial ostium. Revision surgery was performed using a combination of preputial advancement and phallopexy, which resulted in complete and permanent coverage of the glans penis by the prepuce, and at 1 year postoperatively, no recurrence of paraphimosis had been observed. The combined techniques allow preservation of the normal penile anatomy, are relatively simple to perform and provide a cosmetic result. We recommend this combination for the treatment of paraphimosis in the dog, particularly when other techniques have failed.  相似文献   

Theileria orientalis Ikeda genotype infection associated with anaemia,abortion and death in beef cattle in Western Australia     

D Forshaw  SM Alex  DG Palmer  J Cotter  WD Roberts  C Jenkins  S Hair 《Australian veterinary journal》2020,98(7):290-297

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Antimicrobial use and stewardship practices on Australian beef feedlots     

SM Badger  KF Sullivan  D Jordan  CGB Caraguel  SW Page  PMV Cusack  D Frith  DJ Trott 《Australian veterinary journal》2020,98(1-2):37-47

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Cutaneous antimicrobial preparation prior to intravenous catheterization in healthy dogs: clinical, microbiological, and histopathological evaluation.          下载免费PDF全文

B R Coolman  S M Marretta  I Kakoma  M A Wallig  S L Coolman    A J Paul 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(12):757-763

The purpose of this study was to determine the effects of a one-minute chlorhexidine gluconate skin preparation protocol prior to cephalic vein catheterization. Twenty-three healthy beagle dogs had one leg aseptically prepared and the opposite leg served as a control. Twenty-six- and 77-hour time groups were studied. Chlorhexidine-treated legs had significantly lower cutaneous bacterial counts than the control legs prior to catheter insertion and prior to catheter withdrawal for both time groups. Control legs developed significantly more dermatitis than the treated legs after 77 h. A one-minute preparation with 4% chlorhexidine gluconate was an effective method for sustained reduction of cutaneous bacterial counts at peripheral intravenous catheter insertion points in dogs. Increased cutaneous bacterial counts were associated with significantly more microscopic dermatitis in untreated legs after 77 h of catheterization.  相似文献   

The diagnosis and treatment of oronasal fistulas in three cats     

S M Marretta 《Journal of veterinary dentistry》1988,5(1):4-5

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Indigofera spicata (creeping indigo) poisoning of three ponies     

SM Ossedryver  GI Baldwin  BM Stone  RA McKenzie  AW van Eps  S Murray  MT Fletcher 《Australian veterinary journal》2013,91(4):143-149

Three ponies continuously grazed a pasture containing an estimated 24% Indigofera spicata (wet weight basis) for 4–6 weeks in April and May 2004. They developed ataxia, paresis, depression, muscle fasciculations, dysphagia, ptyalism and halitosis. Two also developed corneal opacity. One pony recovered with supportive treatment, but the other two were euthanased and necropsied. Neuropathology was not present in either case, but both livers had periacinar and periportal lymphocytic infiltrations and hydropic degeneration of mid‐zonal hepatocytes, with mild to moderate periacinar necrosis also evident in one. The I. spicata contained 2.66 mg 3‐nitropropionic acid (3‐NPA)/g dry matter and 1.5 mg indospicine/g dry matter. Indospicine, but not 3‐NPA, was detected in serum from both of the euthanased ponies and indospicine was detected in heart, liver and muscle from the one pony in which this assay was performed. The clinical syndrome closely resembled ‘Birdsville horse disease’ caused by I. linnaei and was similar to that reported in horses poisoned by the closely related species I. hendecaphylla and to 3‐NPA poisoning of other animals, including humans. 3‐NPA is thought to cause this neurological syndrome. To our knowledge, this is the first authenticated report of I. spicata poisoning in grazing animals. We also report here the first published evidence that 3‐NPA and indospicine exist in naturalised I. spicata in Australia and of the formation of indospicine residues in tissues of animals grazing paddocks infested with I. spicata.  相似文献   

Oral and Maxillofacial Surgery in Dogs and Cats     

Amalia M. Zacher  Sandra Manfra Marretta 《Veterinary Clinics of North America: Small Animal Practice》2013

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The development and integrity of equine pre‐antral follicles cultured in vitro with follicle‐stimulating hormone (FSH) supplementation          下载免费PDF全文

MC Max  CB Silva  SM González  AG Lindquist  I Búfalo  RG Gomes  F Morotti  CB Costa  TRR Barreiros  LA Lisboa  MM Seneda 《Reproduction in domestic animals》2017,52(5):899-904

This study investigated the effects of different concentrations of FSH (10, 50, 100 and 200 ng/ml) in supplemented MEM+ on the development of equine pre‐antral follicles that were cultured in vitro for 2 or 6 days. The ovaries (n = 5) from mares in seasonal anoestrus were collected from a local abattoir. Ten ovarian tissue fragments of approximately 3 × 3 × 1 mm were obtained from each animal. The fragments were cultured in situ for 2 days (D2) or 6 days (D6) in MEM+ or MEM+ supplemented with FSH at four different concentrations, establishing the following 11 groups: control (D0); MEM + (D2); MEM + (D6); MEM + 10 ng/ml of FSH (D2); MEM + 10 ng/ml of FSH (D6); MEM + 50 ng/ml of FSH (D2); MEM + 50 ng/ml of FSH (D6); MEM + 100 ng/ml of FSH (D2); MEM + 100 ng/ml of FSH (D6); MEM + 200 ng/ml of FSH (D2); and MEM + 200 ng/ml of FSH (D6). Follicles were observed in only 9.65% (388 of 4,018) of the histological sections. Of the 861 follicles evaluated, 488 were in the primordial stage, and 373 were in various developmental stages; 59.7% were morphologically normal. Regarding the integrity of the pre‐antral follicles, the groups with 100 ng/ml FSH of 2‐days culture as well as 50, 100 and 200 ng/ml FSH of 6‐days culture provided the best results. In conclusion, the in vitro culture of abattoir‐derived equine ovarian fragments presented better morphological integrity when supplemented with FSH for 6 days, in comparison with the MEM culture group. However, no clear effects were observed with FSH regarding the promotion of activation from a primordial to a developing follicle.  相似文献   

Clinical management of Brucella suis infection in dogs and implications for public health          下载免费PDF全文

DR James  G Golovsky  JM Thornton  L Goodchild  M Havlicek  P Martin  MB Krockenberger  DJE Marriott  V Ahuja  R Malik  SM Mor 《Australian veterinary journal》2017,95(1-2):19-25

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