Molecular systematics in Chrysanthemum × grandiflorum (Ramat.) Kitamura     

J. Chatterjee  A.K.A. Mandal  S.A. Ranade  Jaime A. Teixeira da Silva  S.K. Datta 《Scientia Horticulturae》2006

An attempt was made to understand the molecular systematics and genetic differences between 10 original chrysanthemum cultivars and 11 mutants. The similarity among the cultivars and mutants varied from 0.17 to 0.90 using RAPD analyses, a simple but efficient method to distinguish cultivars and to assess parentage. Two distinct groups were found. Two cultivars were present as a separate group showing differences from all other cultivars. Mutants with different flower colour could be identified at the molecular level using RAPD technique holding promise to identify unique genes as SCAR markers. A high genetic distance among the different chrysanthemums showed that there exists a possibility of introgressing new and novel genes from the chrysanthemum gene pool.  相似文献   

An unusual paracaecal abscess associated with Fusobacterium necrophorum in a horse     

CJ TREVILLIAN  BH ANDERSON  MG COLLETT 《Australian veterinary journal》1998,76(10):659-662

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Intestinal obstruction by stones in a turtle.          下载免费PDF全文

S C Rahal  C R Teixeira  G B Castro    L C Vulcano 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(6):375-376

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Detection of Leishmania spp. and associated inflammation in ocular‐associated smooth and striated muscles in dogs with patent leishmaniosis     

Carolina Naranjo  Dolors Fondevila  Marta Leiva  Xavier Roura  Teresa Pea 《Veterinary ophthalmology》2010,13(3):139-143

Objective Canine leishmaniosis is a disease characterized by the wide distribution of the parasite throughout the tissues of the host. The purpose of this study was to describe the presence of Leishmania spp. and associated inflammation in ocular‐associated muscles of dogs with patent leishmaniosis. Procedures Smooth muscles (iris dilator muscle, iris sphincter muscle, ciliary muscle, Müller muscle, smooth muscle of the periorbita and smooth muscle of the nictitating membrane) and striated muscles (orbicularis oculi muscle, obliquus dorsalis muscle and dorsal rectus muscle) were evaluated. Routine staining with hematoxylin and eosin and immunohistochemistry to detect Leishmania spp. were performed on tissue sections. Results Granulomatous inflammation was seen surrounding muscular fibers and was composed mainly of macrophages with scattered lymphocytes and plasma cells. This infiltrate could be seen in 52/473 (10.99%) samples of smooth muscle and 36/142 (25.35%) samples of striated muscle. Parasites were detected in 43/473 (9.09%) samples of smooth muscle and in 28/142 (19.71%) samples of striated muscle. Conclusions To the authors’ knowledge, this is the first report assessing the presence of Leishmania spp. and associated infiltrate in intraocular, extraocular and adnexal smooth and striated muscles. The inflammation present in those muscles could contribute to clinical signs already described, such as blepharitis, uveitis, and orbital cellulitis.  相似文献   

Molecular markers useful to discriminate <Emphasis Type="Italic">Coffea arabica</Emphasis> cultivars with high genetic similarity     

Tiago Vieira Sousa  Eveline Teixeira Caixeta  Emilly Ruas Alkimim  Antonio Carlos Baião de Oliveira  Antonio Alves Pereira  Laércio Zambolim  Ney Sussumu Sakiyama 《Euphytica》2017,213(3):75

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Effect of electrical stunning at slaughter on the quality of farmed turbot (Psetta maxima)     

Toby G Knowles  Steve N Brown  Paul D Warriss  Jeff Lines  Ambrose Tinarwo  Marta Sendon 《Aquaculture Research》2008,39(16):1731-1738

Food quality aspects of farmed turbot (Psetta maxima) were compared following two methods of slaughter: the current commercial method, by immersion in an ice slurry, which is then dewatered after approximately 20 min, or by first humanely, electrically stunning the fish using a prototype commercial stunner, before immersion in an ice slurry, which is dewatered after 20 min. Quality was assessed for up to 10 days of storage on ice following slaughter. No differences were found between the slaughter methods in terms of an overall carcass quality: overall appearance, haemorrhage, damage, burst gall bladder, staining of the body cavity by leakage from the gut or damage to the spine. No detectable difference was found between the treatments using the industry standard freshness scoring system, the Quality Index Method. Both groups of fish were classified as ‘Fresh’ after 10 days of storage on ice. Using objective measurements of colour, no differences between fish from either treatment were found in fillet colour. Changes in flesh pH were similar in electrically stunned and traditionally killed fish with a mean pH (±SE) at 2 h post‐mortem of 6.80±0.027 declining to 6.44±0.032 at 24 h post‐mortem. Humane electrical stunning of turbot at slaughter neither detectably improved nor decreased product quality as measured between 1 and 10 days of storage on ice.  相似文献   

Arbuscular mycorrhizal colonization of vascular plants from the Yungas forests, Argentina     

Alejandra Becerra  Marta Cabello  Franco Chiarini 《Annals of Forest Science》2007,64(7):765-772

In Argentina, the Yungas forests are among the ecosystems most affected by human activity, with loss of biodiversity. To assess the mycorrhizal status in these ecosystems, the roots of 41 native plant species belonging to 25 families were collected throughout the year from two sites of the Yungas forests. Roots were washed and stained to study the presence of arbuscular mycorrhizas (AM). Morphological types of arbuscular mycorrhizas (Arum and Paris-type) and colonization patterns were identified and photographed. All plants presented AM colonization. The AM colonization patterns varied from single intracellular aseptate hyphae, coils, appresoria, to vesicles and/or arbuscules. Among the species studied, the Paris-type colonization showed to be dominant. Results confirmed that AM hosts are predominant in the Yungas of South American forests.  相似文献   

Effect of drought on diameter increment of Quercus ilex, Phillyrea latifolia, and Arbutus unedo in a holm oak forest of NE Spain     

Rom Ogaya  Josep Peuelas  Jordi Martínez-Vilalta  Marta Mangirn 《Forest Ecology and Management》2003,180(1-3):175-184

The present study was carried out to elucidate the drought growth responses of Quercus ilex L., Phillyrea latifolia L., Arbutus unedo L., and other accompanying woody species of the Mediterranean holm oak forest. We submitted holm oak forest stands in Prades mountains (NE Spain) to a 2-year experimental drought. We reduced soil water availability about 15% by plastic strips and funnels that partially excluded rain throughfall and by ditch interception of water runoff. Mean stem diameter increment showed a great variation depending on the species. A. unedo had larger growth rates than Q. ilex and P. latifolia, but it was also the species that experimented the highest growth reduction in the drought plots (77%), suggesting a higher drought sensitivity than Q. ilex (55%) and P. latifolia (no drought effect). The growth reduction was specially marked in the larger trees. Aboveground stand biomass increment, estimated from stem diameter by allometric relationships, was 1.9 Mg ha⁻¹ per year in the control plots. The 15% reduction in the upper soil moisture produced 42% reduction in this biomass increment. In the drier conditions predicted in this Mediterranean area in the frame of climate change, an important reduction of growth rates can be hence expected, accompanied by a gain of dominance of drought-tolerant species such as P. latifolia in detriment of more mesic species such as Q. ilex.  相似文献   

Shoot development and dieback in progenies of Nothofagus obliqua     

Javier Puntieri  Javier Grosfeld  Marina Stecconi  Cecilia Brion  María Marta Azpilicueta  Leonardo Gallo  Daniel Barthélémy 《Annals of Forest Science》2007,64(8):839-844

Shoot growth and dieback were compared among progenies of nursery-grown seedlings of Nothofagus obliqua belonging to seven progenies of the same provenance (Quila-Quina, Argentina). First-year shoots consisted of one growth unit (GU) and second-year shoots of one or two GUs. The probability of development of two GU was similar for all progenies. Progenies were different in terms of shoot size, terminal bud abscission, the extent of shoot dieback after shoot extension and the node of origin of the relay shoot on the first shoot. Plants with a second-year shoot consisting of two GUs had a thicker stem and more nodes than those with single-GU shoots. The selection of N. obliqua seed trees based on architectural traits suitable for forestry development at specific sites must contemplate variability among progenies and their probabilities of successful development under different conditions.  相似文献   

Detection of Incipient Germination in Malting Barley with a Starch Viscosity Method and a Proposed Ethanol Emission Method     

Wayne T. Buckley  Marta S. Izydorczyk  William G. Legge 《Cereal Chemistry》2016,93(5):450-455

The risk of germination loss during storage in two‐row malting barley can be reduced by identifying grain lots that have undergone incipient germination (IG) during harvest. A method based on starch viscosity that utilizes a Rapid Visco Analyzer (RVA) is currently available for IG analysis. A new potential method, based on the measurement of ethanol emission (EE) from whole barley, may be more efficient and less expensive than the RVA procedure. Three storage experiments were performed: experiments 1 and 3 at 25°C and 80% rh, and experiment 2 in unheated, uninsulated buildings. Decline in germination energy (GE) varied from <1% to about 80% during storage. In experiments 1 and 3, R² for GE loss (expressed as [weeks to 5% loss of GE]^0.2) versus EE was 0.76 in both cases, whereas R² for GE loss versus RVA viscosity was 0.64 and 0.68, respectively. In experiment 2, the greatest loss of GE was associated with high temperatures and relative humidity in July and August. EE performed as well as RVA in the prediction of germination loss in all three trials. Although further evaluation of the EE method is required, it appears to be a promising alternative to the RVA technique.  相似文献