Metabolic profiling of laser microdissected vascular bundles of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis>     

Schad M  Mungur R  Fiehn O  Kehr J 《Plant methods》2005,1(1):2

Background  

Laser microdissection is a useful tool for collecting tissue-specific samples or even single cells from animal and plant tissue sections. This technique has been successfully employed to study cell type-specific expression at the RNA, and more recently also at the protein level. However, metabolites were not amenable to analysis after laser microdissection, due to the procedures routinely applied for sample preparation. Using standard tissue fixation and embedding protocols to prepare histological sections, metabolites are either efficiently extracted by dehydrating solvents, or washed out by embedding agents.  相似文献   

Antibodies to selected pathogens in free-ranging terrestrial carnivores and marine mammals in Canada   总被引：1，自引：0，他引：1  

Philippa JD  Leighton FA  Daoust PY  Nielsen O  Pagliarulo M  Schwantje H  Shury T  Van Herwijnen R  Martina BE  Kuiken T  Van de Bildt MW  Osterhaus AD 《The Veterinary record》2004,155(5):135-140

Antibody titres to selected pathogens (canine adenovirus [CAV-2], feline herpesvirus [FHV], phocine herpesvirus [PHV-1], canine distemper virus, dolphin morbillivirus [DMV], phocine distemper virus [PDV], parainfluenza virus type 3 [PI3], rabies virus, dolphin rhabdovirus [DRV], canine coronavirus, feline coronavirus, feline leukaemia virus, Borrelia burgdorferi and Toxoplasma gondii) were determined in whole blood or serum samples from selected free-ranging terrestrial carnivores and marine mammals, including cougars (Fellis concolor), lynxes (Fellis lynx), American badgers (Taxidea taxus), fishers (Martes pennanti), wolverines (Gulo gulo), wolves (Canis lupus), black bears (Ursus americanus), grizzly bears (Ursus arctos), polar bears (Ursus maritimus), walruses (Odobenus rosmarus) and belugas (Delphinapterus leucas), which had been collected at several locations in Canada between 1984 and 2001. Antibodies to a number of viruses were detected in species in which these infections have not been reported before, for example, antibodies to CAV-2 in walruses, to PDV in black bears, grizzly bears, polar bears, lynxes and wolves, to DMV in grizzly bears, polar bears, walruses and wolves, to PI3 in black bears and fishers, and to DRV in belugas and walruses.  相似文献   

A "Schrodinger Cat" Superposition State of an Atom   总被引：1，自引：0，他引：1  

C Monroe  DM Meekhof  BE King  DJ Wineland 《Science (New York, N.Y.)》1996,272(5265):1131-1136

A "Schrodinger cat"-like state of matter was generated at the single atom level. A trapped 9Be+ ion was laser-cooled to the zero-point energy and then prepared in a superposition of spatially separated coherent harmonic oscillator states. This state was created by application of a sequence of laser pulses, which entangles internal (electronic) and external (motional) states of the ion. The Schrodinger cat superposition was verified by detection of the quantum mechanical interference between the localized wave packets. This mesoscopic system may provide insight into the fuzzy boundary between the classical and quantum worlds by allowing controlled studies of quantum measurement and quantum decoherence.  相似文献   

In vitro effects of lipopolysaccharides on bovine uterine contractility     

Maraike Wiebe  Christiane Pfarrer  Lara Górriz Martín  Marion Schmicke  Martina Hoedemaker  Heiner Bollwein  Maike Heppelmann 《Reproduction in domestic animals》2021,56(1):172-182

Metritis is an important disorder in dairy cows during the early postpartum period. Myometrial contractility is a prerequisite for uterine involution; however, very scanty literature is available about the effect of metritis on this process and endocrine responsiveness. This study was aimed to evaluate the effect of inflammation on uterine contractility in vitro, and the inflammation was induced by incubating myometrial strips with lipopolysaccharides (LPS). Myometrial samples were collected from 17 healthy Holstein Friesian cows during caesarean section. Eight longitudinal strips from each cow were incubated in organ baths with LPS concentrations of 0 (LPS₀), 0.1 (LPS_0.1), 1 (LPS₁) and 10 µg/ml (LPS₁₀). Spontaneous contractility and contractility induced by increasing concentrations of oxytocin (10^–10 – 10^–7 mol/L) were recorded during nine 30-min intervals (T1 to T9). The minimum amplitude (minA), maximum amplitude (maxA), mean amplitude (meanA) and area under the curve (AUC) were calculated for each time interval. LPS had an effect (p ≤ .05) on maxA, meanA and AUC. In T1, myometrial strips incubated with LPS_0.1 and LPS₁ had higher (p ≤ .05) maxA, meanA and AUC than the strips incubated with LPS₀. In T9 without oxytocin, LPS₀ led to higher (p ≤ .05) maxA, meanA and AUC than LPS_0.1 and LPS₁. In T8 and T9 with oxytocin, LPS₁ had lower (p ≤ .05) maxA, meanA and AUC than the other LPS concentrations. Interestingly, the results show that LPS has a transient positive effect on myometrial contractility in vitro and that this effect is dependent on LPS concentration and duration of incubation.  相似文献   

Smart textiles biotechnology for electrocardiogram monitoring in horses during exercise on treadmill: Validation tests     

Martina Felici  Mimma Nardelli  Antonio Lanatà  Micaela Sgorbini  Enzo Pasquale Scilingo  Paolo Baragli 《Equine veterinary journal》2021,53(2):373-378

  相似文献   

Schätzung des Humusgehaltes nach der Munsell-Farbhelligkeit     

Hans-Peter Blume  Martina Helsper 《植物养料与土壤学杂志》1987,150(5):354-356

Calculation of the humus content of soil samples by the Munsell-value The log values of humus contents (0.1–15%) and the Munsell-values of 156 dry or moist soil samples from Northern Germany were strongly correlated, especially in additional consideration of Munsell-chromas and soil texture.  相似文献   

Der Einsatz von Kationenaustauscherharzen zur Charakterisierung verfügbarer Kationen aus natürlich gelagerten Bodenproben     

Martina K. Zuleger  Rudolf Schierl 《植物养料与土壤学杂志》1990,153(4):277-278

Investigation of cation release of undisturbed soil columns via cation exchange resins Undisturbed soil columns were investigated with cation exchange resins. Similar to roots there is an exchange of H⁺ and cations. The ability for release of cations by molecular diffusion is decreasing with time, so that an asymptotic approximation to a final value occurs. A part of the protons is buffered, for example by aluminium-hydroxides. Samples rich in organic carbon show different graphs for iron and manganese which is probably due to microbial reduction processes.  相似文献   

Bacterial ghost technology for pesticide delivery     

Hatfaludi T  Liska M  Zellinger D  Ousman JP  Szostak M  Ambrus A  Jalava K  Lubitz W 《Journal of agricultural and food chemistry》2004,52(18):5627-5634

Bacterial ghosts are nondenaturated empty cell envelopes of Gram-negative bacteria produced by E-mediated lysis. Such envelopes from the plant-adhering bacterium Pectobacterium cypripedii were tested for their ability to adhere to plant material and to be used as carriers for pesticide delivery. We show, using fluorescence-labeled P. cypripedii ghosts, that depending on the target plants 55 or 10% (rice or soya, respectively) of the applied bacterial ghosts was retained on the leaves after heavy simulated rain (84 mm). Furthermore, the bacterial ghosts could be loaded with the lipophilic triazole fungicide tebuconazole. In subsequent plant experiments in the glass house, the efficacy of the loaded bacterial ghost for resistance to rainfall and the protective and curative effects against the pathogens Erysiphe graminis, Leptosphaeria nodorum, and Pyrenophora teres on barley and wheat and against Sphaerotheca fuliginea on cucumber were tested. The bacterial ghosts were compared primarily with a commercial tebuconazole formulation, a wettable powder, as it has similar physical characteristics. The comparison revealed similar effects and showed consistently higher or comparable efficacy against the pathogens. The standard operational comparison with the most protective, cereal specific emulsion of oil in water displayed that the bacterial ghosts had equal to or lower efficacy than the emulsion. This study confirmed the potential of bacterial ghost platform technology as a new alternative carrier system for pesticides.  相似文献   

Role of riboflavin in beer flavor instability: determination of levels of riboflavin and its origin in beer by fluorometric apoprotein titration     

Duyvis MG  Hilhorst R  Laane C  Evans DJ  Schmedding DJ 《Journal of agricultural and food chemistry》2002,50(6):1548-1552

A method for the quantitative determination of riboflavin levels in beer was developed. The method is based on the quenching of riboflavin fluorescence, which occurs when riboflavin binds to the aporiboflavin-binding protein from egg white. The method does not require any pretreatment of the beer before analysis, other than dilution, and proved to be simple, reliable, and sensitive. The lowest concentration that could be detected was approximately 10 nM riboflavin. The possible interference of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) with the determination of the riboflavin content of beer was excluded, because beer contains only a very small amount of FAD (0.03 microM) and no FMN. The riboflavin levels of the types and brands of beer investigated were in the range of 0.5-1.0 microM. The origin of the riboflavin in beer proved to be the malt. Hop and yeast hardly contributed to the riboflavin content of beer. Besides its use in the determination of riboflavin levels, the aporiboflavin-binding protein also provides a way to remove riboflavin from beer, which reduces the light sensitivity and the related lightstruck off-flavor formation in beer.  相似文献   

Metabolic profiles of the mycotoxin zearalenone and of the growth promoter zeranol in urine,liver, and muscle of heifers     

Kleinova M  Zöllner P  Kahlbacher H  Hochsteiner W  Lindner W 《Journal of agricultural and food chemistry》2002,50(17):4769-4776

A group of five heifers were fed for 84 days with 2 kg of zearalenone-contaminated oats (1370 microg/kg) resulting in an average daily intake of 2740 microg of zearalenone per animal. In a parallel experiment five heifers were implanted with two 25 mg zeranol pellets, one at the beginning of the study and one after 42 days, and fed with 2 kg of "blank" control oats (79 microg/kg, daily intake = 158 microg). A third group of five animals were also fed with 2 kg of "blank" oats and served as control. Urine samples of all animals were collected every 5-6 days during the whole period of the study. Animals of all three groups were killed 84 days after the beginning of the feeding study. Tissue samples (back, femoral region, liver, and residues of implanted pellets) were taken during post-mortem investigations. The content of zearalenone and zeranol and their metabolites in urine and tissue samples was established by an analytical method combining solid-phase extraction and high-performance liquid chromatography-tandem mass spectrometry. Urinary excretion rates of zeralenone and zeranol were calculated from these results.  相似文献