Neuromyotonia in a horse     

Luiza Stachewski Zakia  Mariana Isa Pocci Palumbo  Raffaella Bertoni Cavalcanti Teixeira  Luiz Antnio Lima Resende  Mauro Pereira Soares  Jos Paes de Oliveira‐Filho  Rogrio Martins Amorim  Alexandre Secorun Borges 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(1):287-291

This article describes the clinical and electromyographic findings of neuromyotonia in a 19‐month‐old male crossbred Quarter Horse that presented with stiffness and muscle asymmetry in the hind limbs as well as sacrococcygeal, paravertebral, and gluteal myokymia. An electromyographic study showed spontaneous continuous muscle fiber activity with high‐frequency discharges, fibrillations, positive sharp waves, fasciculation potentials, and complex repetitive discharges. Histological examination of the gluteal muscle showed a mixed neurogenic and myopathic pattern. The findings are consistent with neuromyotonia.  相似文献   

Antimicrobial susceptibility of Staphylococci isolated from naturally occurring canine external ocular diseases     

Renato Varges  Bruno Penna  Gabriel Martins  Rodrigo Martins  Walter Lilenbaum 《Veterinary ophthalmology》2009,12(4):216-220

Objective  The purpose of this study was to describe the species distribution and antimicrobial susceptibility patterns of Staphylococci isolated from naturally occurring canine ocular diseases.
Samples and Procedures  Samples obtained from 68 dogs with signs of external eye disease were processed for isolation of bacteria of the genus Staphylococcus sp. Isolates were identified by biochemical reactions and tested for susceptibility to 11 antimicrobials of six classes.
Results  Forty (58.8%) samples yielded Staphylococcus sp. in pure culture. Coagulase-positive Staphylococci were most common and Staphylococcus intermedius was the most frequent (45%) species, followed by S . aureus (22.5%) and the coagulase-negative species S. epidermidis (20%), and S . simulans (12.5%). Resistance to at least one drug was observed in 92.5% of the isolates, and multidrug resistance was a common finding (72.5%). The most effective drugs against Staphylococcus strains isolated from extra-ocular canine ocular diseases were ceftiofur and cefalexin.
Conclusion  This study highlights the presence of Staphylococcus sp. genus in naturally occurring extra-ocular canine ocular disease and the emergence of resistant strains to common antimicrobial drugs. It also emphasizes the need for bacterial culture with species identification and susceptibility testing in order to choose the appropriate antimicrobial therapy.  相似文献   

Stressing fish in Recirculating Aquaculture Systems (RAS): does stress induced in one group of fish affect the feeding motivation of other fish sharing the same RAS?     

Catarina I M Martins  Ep H Eding  Johan A J Verreth 《Aquaculture Research》2011,42(9):1378-1384

As a consequence of water re‐use and high stocking densities, Recirculating Aquaculture Systems (RAS) may lead to an accumulation of substances released by the fish into the water, e.g. cortisol and alarm pheromones. This study investigated the effect of stressing fish on the feeding motivation of other fish not subjected to stress but sharing the same water of stressed fish. Two identical RAS were used (operated at 30 L kg feed^?1 day^?1) and contained grouped (stressed fish) and individually (receiving water from stressed fish) housed Nile tilapia. A stress test was applied in grouped housed fish on days 17 and 55. Feeding behaviour (intake and latency) was recorded in the individually housed fish 3 days before, during and 3 days after the stress test. The results showed no effect on feeding behaviour in fish receiving the water from stressed fish. These results could be a consequence of insufficient cortisol/alarm cues' release by the stressed fish into the water or inactivity of such substances, either due to a trapping effect of humic acids or due to degradation in the nitrification and denitrification processes. Future research is needed to clarify how these processes may affect the water concentration of cortisol and alarm pheromones and should be extended by measuring other behavioural and physiological traits.  相似文献   

Use of probiotic bacteria in the rearing of Senegalese sole (Solea senegalensis) larvae   总被引：1，自引：0，他引：1  

Pavlos Makridis  Sílvia Martins  João Reis  & Maria Teresa Dinis 《Aquaculture Research》2008,39(6):627-634

Three candidate probiotics, which had shown antimicrobial activity in vitro against two fish pathogens, were used in the rearing of Senegalese sole larvae and postlarvae. These probiotics improved the survival of starved sole yolk‐sac larvae. A feeding experiment of sole larvae and postlarvae comprised three treatments: A, B and C. Cultures of a Vibrio sp. (2J18) were added to treatment A, whereas a gram‐positive (J84) and a Shewanella sp. strain (2J27), were added to treatment B, while in a control treatment C no bacteria were added. Addition of bacteria in treatment B increased survival of larvae in the first phase of the experiment [0–20 days after hatching (DAH) (P<0.05)] and decreased the numbers of colony‐forming units (CFU) in larval gut 5 DAH compared with the control treatment (P<0.05). No differences were observed in survival (25–47%) during the second phase of the experiment (20–60 DAH). Nevertheless, the total numbers of CFU in fish gut 40 DAH were significantly lower in treatment B (P<0.05). The colonization rates of the added bacteria were the highest 5 and 11 DAH, and the highest average values reached were 65%, 82% and 17% of the total CFU count for the strains 2J18, 2J27 and J84 respectively.  相似文献   

Gender status effect on carcass and meat quality traits of feedlot Angus × Nellore cattle     

Lenise Freitas Mueller  Júlio Csar Carvalho Balieiro  Adrielle Matias Ferrinho  Taiane da Silva Martins  Rosana Ruegger Pereira da Silva Corte  Tamyres Rodrigues de Amorim  Joyce de Jesus Mangini Furlan  Fernando Baldi  Anglica Simone Cravo Pereira 《Animal Science Journal》2019,90(8):1078-1089

The study evaluated the effect of gender status on carcass and meat quality of feedlot Angus × Nellore cattle. A total of 176 cattle, 20 months old, were confined for 190‐days and assigned to four treatments: bulls, immunocastrated, steers, and heifers. Bulls had greater rib eye area and HCW (p = 0.0001). Heifers had increased fat thickness (p = 0.0001). Steers and heifers had higher marbling scores (p = 0.0001). There was interaction between gender and aging time for Warner‐Bratzler Shear Force (p = 0.0002), L* (p = 0.0118), and b* (p = 0.0113) values of beef. The sensory panel results showed that beef from bulls had the lowest consumer overall acceptance (p = 0.0278). Especially, regardless tenderness, steers and immunocastrated beef were considered tender, independent of aging time. Beef produced by heifers, steers, and immunocastrated is considered to be of higher quality than bulls. Thus, it is may be an interesting alternative to produce high‐quality beef than bulls, to attend the consumer demand for high‐quality products. Additionally, the low fatty acids n6 levels and low n6:n3 ratio, high levels of CLA, MUFAs, and oleic acid suggests that the heifer meat is favorable for human health.  相似文献   

BDDE-Inspired Chalcone Derivatives to Fight Bacterial and Fungal Infections     

Ana Jesus  Fernando Dures  Nikoletta Szemerdi  Joana Freitas-Silva  Paulo Martins da Costa  Eugnia Pinto  Madalena Pinto  Gabriella Spengler  Emília Sousa  Honorina Cidade 《Marine drugs》2022,20(5)

The growing number of infectious diseases around the world threatens the effective response of antibiotics, contributing to the increase in antibiotic resistance seen as a global health problem. Currently, one of the main challenges in antimicrobial drug discovery is the search for new compounds that not only exhibit antimicrobial activity, but can also potentiate the antimicrobial activity and revert antibiotics’ resistance, through the interference with several mechanisms, including the inhibition of efflux pumps (EPs) and biofilm formation. Inspired by macroalgae brominated bromophenol BDDE with antimicrobial activity, a series of 18 chalcone derivatives, including seven chalcones (9–15), six dihydrochalcones (16–18, and 22–24) and five diarylpropanes (19–21, and 25 and 26), was prepared and evaluated for its antimicrobial activity and potential to fight antibiotic resistance. Among them, chalcones 13 and 14 showed promising antifungal activity against the dermatophyte clinical strain of Trichophyton rubrum, and all compounds reversed the resistance to vancomycin in Enterococcus faecalis B3/101, with 9, 14, and 24 able to cause a four-fold decrease in the MIC of vancomycin against this strain. Compounds 17–24 displayed inhibition of EPs and the formation of biofilm by S. aureus 272123, suggesting that these compounds are inhibiting the EPs responsible for the extrusion of molecules involved in biofilm-related mechanisms. Interestingly, compounds 17–24 did not show cytotoxicity in mouse embryonic fibroblast cell lines (NIH/3T3). Overall, the results obtained suggest the potential of dihydrochalcones 16–18 and 22–24, and diarylpropanes 19–21, 25 and 26, as hits for bacterial EPs inhibition, as they are effective in the inhibition of EPs, but present other features that are important in this matter, such as the lack of antibacterial activity and cytotoxicity.  相似文献