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61.
Beth A Valentine Wilson K Rumbeiha Terry S Hensley Richard R Halse 《Journal of veterinary diagnostic investigation》2007,19(2):212-215
Over a 12-day period, 13 animals in a herd of 110 beef cattle developed ataxia with profound muscle fasciculations progressing to recumbency. Twelve animals (5 adults and 7 calves from 8-10 months of age) died, and 1 cow was euthanized. Hemorrhagic diarrhea occurred in some, but not all, animals. The onset of clinical signs was at least 12 hours after the cattle had gained access to contents of old buildings used for storage, and the majority of deaths occurred within 24 to 48 hours after the onset of clinical signs. Approximately 9 kg of unidentified pellets were found strewn in the barn area where the cattle had been. Autolysis considered more severe than expected for the postmortem interval, suggestive of high body temperature before death, and congestion of body tissues were the only significant findings detected in the cow that was euthanized and submitted for necropsy examination. The clinical history and lack of postmortem lesions were most consistent with toxicity. A toxic level of arsenic (6.18 ppm) was detected in the kidney, and metaldehyde was detected in the liver. The pellets were analyzed and found to contain both arsenic and metaldehyde, consistent with a discontinued molluscicidal product. 相似文献
62.
Mary Lynn Johnson Jessica M Evoniuk Charles L Stoltenow Katherine I O'rourke Dale A Redmer 《Journal of veterinary diagnostic investigation》2007,19(1):73-77
The objective of this study was to develop a reliable Taqman 5' Nuclease Assay for genotyping sheep for scrapie susceptibility. The sheep prion gene contains 2 single nucleotide polymorphisms (SNPs) that may mediate resistance to classical scrapie, one at codon 136, alanine (A) or valine (V), and another at codon 171, arginine (R) or glutamine (Q). The R allele appears to confer resistance to classical scrapie, with the AA(136) RR(171) genotype the most resistant to scrapie and QR(171) only rarely infected in the US sheep population. The Assays by Design protocol was used for development of probes and primers for codon 136 and Primer Express for codon 171. Commercially available kits were used to isolate genomic DNA from blood or muscle. For validation, 70 SNP determinations for each codon were compared to commercial testing with an error rate of less than 1%. Then, 935 samples from blood (n = 818) and muscle (n = 117) were tested for both codons with 928 successful determinations and only 7 samples (<1% of total samples) that needed repeating. Genotypes were AA QQ (n = 102; 11.0%), AV QQ (n = 28; 3.0%), AA QR (n = 396; 42.7%), AV QR (n = 54; 5.8%), and AA RR (n = 348; 37.5%). Thus, 86% of the sheep tested (n = 798) contained R at codon 171 and were expected to be scrapie-resistant. This new Taqman 5' Nuclease SNP genotyping assay is accurate, easy to perform, and useful in the study of classical scrapie in sheep and its prevention through selective breeding programs to eliminate highly susceptible animals. 相似文献
63.
64.
M Glade Weiser Linda M Vap Mary Anna Thrall 《Veterinary Clinics of North America: Small Animal Practice》2007,37(2):221-36, v
The typical technologies used in veterinary hematology and biochemical analyzers are reviewed, along with associated advantages and disadvantages. Guidelines for implementing a successful in-clinic laboratory are provided, including criteria for system evaluation and expectations for comparative performance evaluations. The more common problems and limitations associated with in-clinic laboratory diagnostics and how to best prevent them are also discussed. 相似文献
65.
Spatial and temporal landscape patterns have long been recognized to influence biological processes, but these processes often
operate at scales that are difficult to study by conventional means. Inferences from genetic markers can overcome some of
these limitations. We used a landscape genetics approach to test hypotheses concerning landscape processes influencing the
demography of Lahontan cutthroat trout in a complex stream network in the Great Basin desert of the western US. Predictions
were tested with population- and individual-based analyses of microsatellite DNA variation, reflecting patterns of dispersal,
population stability, and local effective population sizes. Complementary genetic inferences suggested samples from migratory
corridors housed a mixture of fish from tributaries, as predicted based on assumed migratory life histories in those habitats.
Also as predicted, populations presumed to have greater proportions of migratory fish or from physically connected, large,
or high quality habitats had higher genetic variability and reduced genetic differentiation from other populations. Populations
thought to contain largely non-migratory individuals generally showed the opposite pattern, suggesting behavioral isolation.
Estimated effective sizes were small, and we identified significant and severe genetic bottlenecks in several populations
that were isolated, recently founded, or that inhabit streams that desiccate frequently. Overall, this work suggested that
Lahontan cutthroat trout populations in stream networks are affected by a combination of landscape and metapopulation processes.
Results also demonstrated that genetic patterns can reveal unexpected processes, even within a system that is well studied
from a conventional ecological perspective. 相似文献
66.
The riverscape perspective recognizes the heterogeneous habitat types within the stream corridor as a single, integrated ecological
unit operating across spatial scales. Although there is ample evidence that the riverscape notion is appropriate in understanding
the physical phenomena of stream corridors, significantly less attention has focused on its ecological ramifications. To this
end, we surveyed riverscape habitat variables and bird community characteristics in the Champlain Valley of Vermont, USA.
From the data collected, we used information theoretic methodology (AICc) to model relationships between bird community attributes and key habitat variables across the riverscape. Our models with
the greatest support suggest that riverine bird communities respond to a suite of characteristics; representing a variety
of riverscape habitats at the in-stream, floodplain, and riparian levels. Channel slope, drainage area, percent conifers,
and in-stream habitat condition were among the most influential variables. We found that piscivores are potentially important
indicators of riverscape condition, responding to a host of variables across the riverscape. Our results endorse a holistic
approach to assessing and managing the mosaic of patches in the riverscape and suggest that a riverscape approach has significant
conservation potential. 相似文献
67.
James E. Childs Laura E. Robinson Ramses Sadek Anthony Madden Mary Elizabeth Miranda Noel L. Miranda 《Preventive veterinary medicine》1998,33(1-4):207-218
We estimated the population density of dogs by distance sampling and assessed the potential utility of two marking methods for capture-mark-recapture applications following a mass canine rabies-vaccination campaign in Sorsogon Province, the Republic of the Philippines. Thirty villages selected to assess vaccine coverage and for dog surveys were visited 1 to 11 days after the vaccinating team. Measurements of the distance of dogs or groups of dogs from transect lines were obtained in 1088 instances (N = 1278 dogs; mean group SIZE = 1.2). Various functions modelling the probability of detection were fitted to a truncated distribution of distances of dogs from transect lines. A hazard rate model provided the best fit and an overall estimate of dog-population density of 468/km2 (95% confidence interval, 359 to 611). At vaccination, most dogs were marked with either a paint stick or a black plastic collar. Overall, 34.8% of 2167 and 28.5% of 2115 dogs could be accurately identified as wearing a collar or showing a paint mark; 49.1% of the dogs had either mark. Increasing time interval between vaccination-team visit and dog survey and increasing distance from transect line were inversely associated with the probability of observing a paint mark. Probability of observing a collar was positively associated with increasing estimated density of the dog population in a given village and with animals not associated with a house. The data indicate that distance sampling is a relatively simple and adaptable method for estimating dog-population density and is not prone to problems associated with meeting some model assumptions inherent to mark-recapture estimators. 相似文献
68.
A Syndrome Resembling Idiopathic Noncirrhotic Portal Hypertension in 4 Young Doberman Pinschers 总被引:1,自引:0,他引:1
JoAnn DeMarco Sharon A. Center Nathan Dykes Amy E. Yeager Bruce Kornreich Ed Gschrey Kelly A. Credille Magali Guffroy Fabio del Piero Beth A. Valentine 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):147-156
We describe 4 young male Doberman Pinschers (3 littermates and 1 unrelated dog) with a syndrome resembling idiopathic or noncirrhotic portal hypertension of humans. Each dog was evaluated for a hepatopathy resulting in portal hypertension, development of portosystemic collateral vessels, and hepatic encephalopathy. These dogs differ from previous reports of young dogs with hepatic insufficiency associated with portal hypertension and acquired portal systemic shunting by their lack of intrahepatic arteriovenous fistulae, portal vein atresia, or intrahepatic fibrosis. Clinicopathologic features included erythrocyte microcytosis, normal to mildly increased liver enzyme activities, increased concentrations of serum bile acids, reduced plasma indocyanine green clearance, and normal total bilirubin concentration. Abdominal ultrasonography disclosed a small liver and portosystemic collateral vessels. Radiographic imaging studies confirmed hepatofugal portal circulation and discounted hepatic arteriovenous fistulae. Histopathologic features in liver tissue from each dog were similar and consistent in all sections examined. Common findings included increased cross-sectional views of hepatic arterioles; hepatic lobular atrophy; scanty increase in connective tissue around some large portal triads; and absence of inflammation, disturbed lobular architecture, bile duct proliferation, or intrahepatic cholestasis. 相似文献
69.
70.
Martin Furr Mary Kay Tinker Lucy Edens 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1997,11(3):183-188
This study was conducted to develop an equation for the prediction of outcome in neonatal foals undergoing treatment in an intensive care unit (ICU). Fifty-three physical examination, historical, and clinicopathologic variables were analyzed from the records of 99 neonatal foals (<14 days of age) treated in the neonatal ICU of the Equine Medical Center. The outcome was recorded and the results were categorized into either surviving or nonsurviving groups. The mean values for the 2 groups were compared, and variables that differed significantly between the two groups were retained and used to construct a logistic regression equation. Retained variables were heart rate, temperature, and neutron phil count. The predictive equation then was tested prospectively in 2 additional groups of foals from 2 separate ICUs. The predicted outcome was compared to the actual outcome, and performance variables were calculated. Sensitivity (.83), specificity (.87), negative predictive value (.72), and positive predictive value (.93) were determined for foals from one neonatal ICU; the sensitivity (.83), specificity (.44), negative predictive value (.44), and positive predictive value (.83) were lower for foals at a second, separate ICU. 相似文献