Intrafollicular Concentrations of Steroid Hormones and PGF2α in Relation to Follicular Development in the Mares during the Breeding Season     

Tsutomu TSUKADA  Anna Yumiko KOJIMA  Kunitada SATO  Masaharu MORIYOSHI  Masanori KOYAGO  Yutaka SAWAMUKAI 《Journal of Equine Science》2008,19(2):31-34

The concentrations of androstenedione, estradiol-17β, progesterone and PGF_2α contained in the follicular fluid produced by the follicles in collected ovaries of mares that have had estrous phase during the breeding season were measured and analyzed the relation between the growth stage of follicles and the hormone levels in the follicular fluid. An ultrasonographic diagnostic instrument was used to measure the diameter of the follicles in order to categorize the follicles into three groups the following: 8 small follicles (from 1.0 to less than1.5 cm), 8 medium follicles (from 1.5 to less than 3.0 cm), and 8 large follicles (from 3.0 to 5.0 cm), respectively. The analysis of the follicular fluid in ovaries of estrous mares showed that the concentrations of androstenedione were significantly higher in the medium or large follicles than in the small follicles and the concentrations of estradiol-17β were significantly higher in larger follicles than in the small or medium follicles (P<0.05). The concentrations of progesterone and PGF_2α, on the other hand, did not significantly vary regardless of follicluar size. In the follicles within the mare ovaries that have had estrous stage, the concentrations of the hormones related the ovulation, namely androstenedione and estradiol-17β, were higher with larger follicles.  相似文献   

Spermatozoa morphology during the breeding season in Thoroughbred stallions in Japan     

Koyago M  Nakada K  Tsunoda N  Moriyoshi M  Sawamukai Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1121-1124

The morphology of spermatozoa of modern Thoroughbred stallions in Japan was investigated during the breeding season. A total of 299 semen samples were collected from the penises of 16 stallions immediately after service. The rate of abnormalities in sperm heads and tails, spermatozoa with cytoplasmic droplets and slides with medusa cells to total observed slides in each stallion were 3.9 +/- 2.1%, 11.5 +/- 5.9%, 2.4 +/- 2.6% and 20.1%, respectively. The values for the area, length, width and aspect ratio of the stallion sperm head were 12.54 +/- 1.34 microm(2), 5.93 +/- 0.40 microm, 2.69 +/- 0.21 microm and 0.46 +/- 0.05, respectively. With the exception of medusa cells, the features were significantly different among the stallions (P<0.05).  相似文献   

A case of atypical canine lymphoma with oral mass and multiple osteolysis     

Ito T  Hisasue M  Neo S  Ishikawa T  Shida T  Watanabe T  Enomoto Y  Okuda M  Tsuchiya R  Madarame H  Ogata M  Yamada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):977-980

An 8-year-old female Golden Retriever had an oral mass and lameness. Multiple osteolysis of the systemic skeleton without monoclonal gammopathy was shown on electrophoresis of serum and urine samples. Cytological and histopathological examinations of the oral mass revealed atypical polymorphic cells similar to myeloid cells, and bone marrow aspiration indicated that these abnormal cells also might have invaded the bone marrow. These cells were negative to peroxidase and non-specific esterase staining, and clonal expansion of B lymphocytes could be detected by polymerase chain reaction (PCR) assay for antigen receptor gene rearrangement. The case was diagnosed as atypical lymphoma and treated by multi-drug chemotherapy. On the 142nd day after the first admission, the case had remission and the oral mass and multiple osteolysis were improved.  相似文献   

Dry shipper cryopreservation of seven‐band grouper (Epinephelus septemfasciatus Thunberg) spermatozoa     

Ivan Chong Chu Koh  Daisuke Tanaka  Takayoshi Itagane  Masaharu Tsuji  Yasushi Tsuchihashi  Hiromi Ohta 《Aquaculture Research》2012,44(1):59-66

This study examined the usage of a dry shipper for cryopreservation of Epinephelus septemfasciatus (Thunberg) spermatozoa. Milt was diluted 1:49 with 5% dimethyl sulfoxide plus 95% foetal bovine serum for cryopreservation. Computer‐assisted sperm analysis was used to analyse sperm motility, while fertilization and hatching trials were conducted to gauge the applicability of the cryopreservation method for aquaculture. We showed that cooling rates of the dry shipper were stable for 14 days and could be manipulated by the use of different sized freezing straws and use of a simple polystyrene foam container (5 × 5 × 12 cm and 1 cm thickness on all sides with the upper layer exposed). Dry shipper cryopreserved spermatozoa had significantly lower post‐thaw per cent motility and velocity than fresh sperm, but linearity of movement was unchanged. Fertilization and hatching rates were not significantly different at all tested sperm to egg ratios (3000:1–243000:1). The results indicated that 0.33 mL of milt when cryopreserved was sufficient to fertilize up to 450 g of oocytes. Application of this technology will help improve seed production in aquaculture and further develop breeding and genetics studies.  相似文献   

Fatty acid composition in sea cucumber Apostichopus japonicus fed with microbially degraded dietary sources     

Natsuki Hasegawa  Sayumi Sawaguchi  Masaharu Tokuda  Tatsuya Unuma 《Aquaculture Research》2014,45(12):2021-2031

Fatty acids (FAs) in the body walls of sea cucumber, Apostichopus japonicus, reared under diet‐administered conditions were analysed to determine the accumulative status of dietary FAs. Four test diets different in diet source and microbial degradation, that is, a diet composed of powdered pennate diatoms (diet PD), one microbially degraded of diet PD (diet PDM), one composed of brown algae [tangle weed, Saccharina (Laminaria) longissima (Miyabe); diet BA], and one microbially degraded of diet BA (diet BAM), were fed to juvenile A. japonicus, respectively, for 2 months and the FA composition of the juvenile body wall was determined. FAs in body wall were influenced and changed by diet source and microbial degradation of diet. Degradation significantly promoted juvenile's growth and increased iso‐15:0 in the body wall; however, iso‐15:0 was not a growth factor. FA 20:4n6 and 20:5n3 were dominant in all groups despite being a minor dietary constituent, suggesting that these FAs can be readily accumulated or synthesized in the animal.  相似文献   

Phylogenetic characterization of Isospora jaracimrmani oocysts from a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) reared at a zoo in Ishikawa,Japan     

Fitrine EKAWASTI  Kazuya KITAGAWA  Hiroshi DOMAE  April Hari WARDHANA  Junki NAGASAWA  Tomoyuki SHIBAHARA  Masaharu TOKORO  Kazumi SASAI  Makoto MATSUBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1240

Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months.  相似文献   

Isolation and characterization of bacteria causing mortality in early stage larvae of captive‐bred Japanese eels (Anguilla japonica Temminck & Schlegel)          下载免费PDF全文

Gentoku Nakase  Masaharu Tokuda  Kazuharu Nomura  Hideki Tanaka 《Aquaculture Research》2015,46(11):2637-2643

Bacteria with lethal effect on eel larvae were isolated from moribund captive‐bred eel larvae and their 16S rRNA gene sequences were analysed. Nucleotide sequence of 10 isolates showed highest similarity with Lacinutrix algicola, Crocinitomix catalasitica and Pseudoalteromonas rubra. Age‐dependent changes in the susceptibility of eel larvae were observed in response to challenge by a highly lethal isolate. Compared with 10 and 11 days after hatching (DAH), larval susceptibility to the highly lethal isolate was lower at 18 DAH. We found that the bacterial isolates have lethal effect on the captive‐bred eel larvae, especially at the early developmental stage. These results will be useful to establish appropriate culture practices for eel larvae that will improve the success of mass production of glass eels for aquaculture.  相似文献   

Moisture Distribution and Diffusion in Cooked Spaghetti Studied by NMR Imaging and Diffusion Model     

Akemi K. Horigane  Shigehiro Naito  Mamoru Kurimoto  Kentaro Irie  Masaharu Yamada  Hirofumi Motoi  Mitsuru Yoshida 《Cereal Chemistry》2006,83(3):235-242

Moisture diffusion in spaghetti during boiling and holding after boiling was analyzed with a cylinder diffusion model based on moisture content mapped by magnetic resonance imaging. The images showed water penetrating concentrically in the cross section of a cooked spaghetti strand. The moisture profiles calculated from the images refracted near the central part in undercooked spaghetti due to the presence of ungelatinized starch. The moisture profiles of the samples with different holding times intersected around one‐third of the radius from the surface. The slope of the moisture profile decreased along with the holding time while the moisture content at the intersection remained unchanged. The diffusion coefficients were determined statistically for the fully gelatinized region with a cylinder model. The theoretical moisture profiles calculated using such coefficients fitted very well to experimental data. The diffusion coefficients of dried spaghetti were smaller than those of fresh spaghetti at both boiling and holding periods, suggesting that the moisture transport property had been reduced by the drying process during manufacture of spaghetti. The diffusion coefficients decreased with increases in boiling and holding times. The diffusion model with a constant diffusion coefficient is, therefore, valid in a limited boiling or holding time.  相似文献   

Structure of Lysophosphatidylglyceryl-Remazolbrilliant Blue (LPG-RBB) from the Surface of Dyed Wheat Starch Granules     

Masaharu Seguchi  Kazuko Kanenaga 《Cereal Chemistry》1998,75(3):294-296

Wheat starch granule surface was covalently stained with Remazolbrilliant blue-R dye (RBB) and then extracted with 1% SDS containing 1% 2-mercaptoethanol (2-ME) at room temperature for 14.5 hr. The extracted blue-staining material (A₆₅₀) separated into two fractions. Low molecular weight (LMW) material was further purified by Sephadex G-75 size-exclusion chromatography and thin-layer chromatography. Infrared and nuclear magnetic resonance (¹H-NMR and ¹³C-NMR) spectroscopy indicated that the structure of the purified LMW material was 18-O-(6-lysophosphatidylglyceryl)-RBB.  相似文献   

Efficacy of taurine supplementation for preventing green liver syndrome and improving growth performance in yearling red sea bream Pagrus major fed low-fishmeal diet   总被引：1，自引：0，他引：1  

Shusaku Takagi  Hisashi Murata  Takanobu Goto  Toshiaki Ichiki  Makoto Endo  Hideo Hatate  Terutoyo Yoshida  Tadashi Sakai  Hirofumi Yamashita  Masaharu Ukawa 《Fisheries Science》2006,72(6):1191-1199

ABSTRACT:   This study was performed to evaluate the efficacy of taurine supplementation for preventing green liver syndrome and improving growth performance in red sea bream Pagrus major fed a low-fishmeal (FM) diet. Yearling red sea bream were fed for 34 weeks on low-FM diets either supplemented with taurine, or without taurine, and the tissue taurine and bile pigment concentrations were measured. Compared to the fish fed the FM diet, fish fed the low-FM diet without taurine supplementation resulted in inferior feed performances and higher incidence of green liver related to the morphological transformation of the erythrocytes. In these fish, the hepatopancreatic taurine concentration was significantly lower and hepatopancreatic biliverdin concentration was high compared to the fish fed the FM diet. These parameters were markedly improved by taurine supplementation of the low-FM diet and were similar in levels to the fish fed the FM diet. These results indicate that green liver appearance and inferior feed performances of red sea bream fed the low-FM diet without taurine supplementation were caused by dietary taurine deficiency, and indicate the requirement of taurine supplementation to low-FM diets for red sea bream.  相似文献