First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens     

Tomoo Misawa  Masaharu Kubota  Jun Sasaki  Shiro Kuninaga 《Journal of General Plant Pathology》2015,81(1):15-23

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Effect of Gaseous Acetic Acid Treatment on Breadmaking Properties of Flour     

Machiko Hayashi  Masaharu Seguchi 《Cereal Chemistry》1999,76(6):898-901

Eleven different wheat flours of varying protein content were treated with gaseous acetic acid. Each wheat flour exhibited maximum breadmaking properties (bread height and specific volume) at different levels of gaseous acetic acid treatment. There was a relationship (r = +0.7384) between the level of gaseous acetic acid treatment required for maximum breadmaking properties and the protein content of the wheat flour. Wheat flours with higher protein contents were more resistant to the decrease in pH value due to acetic acid treatment.  相似文献   

Analytical characterization of 2-bromo-palmitic acid by gas chromatography–mass spectrometry as a tool for analysis of lipid metabolism in fish     

Tokuda  Masaharu  Oku  Hiromi 《Fisheries Science》2009,75(2):533-535

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Zoogeographical barriers causing discontinuous osteometrical variations in the northern treeshrew skulls     

Hideki ENDO  Truong Son NGUYEN  Dinh Duy NGUYEN  Motoki SASAKI  Junpei KIMURA  Tatsuo OSHIDA  Masaya YAGO  Ngoc Tu LY  Thi Tham NGUYEN  Masaharu MOTOKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1534

Morphological variation of the skull was examined in the northern treeshrew (Tupaia belangeri) from various localities across Southeast Asia. Through a multivariate analysis, the treeshrews from South Vietnam exhibited distinct morphological characteristics compared to other populations from Thailand and Laos, and Malaysia. The plots of the specimens of North Vietnam are not randomly mixed with Thailand plots segregation in the scatteregrams of canonical discriminant analysis. Since the skulls of the population from North Vietnam were morphologically similar to those form central Laos and northern and northeastern Thailand, the zoogeographical barrier effect of Mekong River was not clearly confirmed. The population of the Kanchanaburi in western Thailand is clearly smaller in size compared to the other populations. The southern border of the distribution of this species is determined by the Isthmus of Kra or Kangar-Pattani Line. In the northern treeshrew, which is distributed from southern China to Bangladesh and southern Thailand, we have detected osteometrical geographical variation driven by geography. These results indicate that the skull morphology in the Tupaia glis-belangeri complex distinctively differs in South Vietnam, western Thailand, and southern Thailand. The zoogeographical barrier and factor separating these districts are expected to clarify in the future.  相似文献   

Effect of Gaseous Acetic Acid on Dough Rheological and Breadmaking Properties     

Masaharu Seguchi  Machiko Hayashi  Hiroshi Matsumoto 《Cereal Chemistry》1997,74(2):129-134

Breads baked from wheat flours (protein contents 14.1–16.5% at 14.0% mb) that were pretreated with 2–3 mL of gaseous acetic acid per kg of wheat flour, showed maximum bread height and specific volume (cm³/g). Flour-water suspension and the crumb pH values were gradually decreased with increased amounts of acetic acid. Gas generation and dough expansion tests with bread dough showed that the addition of the same amount of acetic acid, which achieved maximum specific volume, also showed the highest rate of gas generation and dough expansion. However, increasing acetic acid decreased these values. Scanning electron microscope (Cryo-SEM) observation showed that the bread dough made from the same acetic acid-treated flour indicated continuum and no cracks in the dough matrix. Evaluation of mixograms showed the decrease of mixing stability with increased acetic acid levels. Viscosity and water binding capacity of flour-water suspensions were sharply increased by the addition of acetic acid at pH 5.0–3.5.  相似文献   

Study of Three-Dimensional Structure of Wheat Starch Granules Stained with Remazolbrilliant Blue Dye and Extracted with Aqueous Sodium Dodecyl Sulfate and Mercaptoethanol Solution     

Masaharu Seguchi  Kazuko Kanenaga 《Cereal Chemistry》1997,74(5):548-552

Wheat starch granules were obtained from soft wheat flour by acetic acid fractionation (pH 3.5), and the starch was stained by reaction with Remazolbrilliant blue (RBB) dye. RBB-stained starch was extracted with 1% sodium dodecyl sulfate (SDS) and 1% 2-mercaptoethanol (ME) for 14.5 hr at room temperature. This extraction step was repeated five times (extracts 1–5). SDS-ME extracts were subjected to size-exclusion column chromatography, and comparisons of their profiles for specific absorbance at 650 nm (A₆₅₀) and carbohydrates were made. After high molecular weight (HMW) carbohydrates on the starch granule surface were extracted, HMW carbohydrates inside the granule appeared to be extracted. Finally, low molecular weight (LMW) carbohydrates near the granule surface were extracted. Phase-contrast light microscopy of the treated starch granules showed that all granules became transparent. Two different interior structures were observed. Scanning electron microscopy indicated that the granule was split into two parts at the equatorial groove. The interior of the granule showed two different areas: a central hole area and the surrounding stratified area. Extraction beyond five times with the same solvent dissolved the weak part of the granule structure and left two types of skeletal structures. The appearance of the skeletal structure of the granule surface was different from the appearance of interior structures.  相似文献   

A cat with myelodysplastic syndrome by administration of the methylation inhibitor Azacytidine     

Masaharu HISASUE  Mina TANAKA  Sakurako NEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):142

A 5-year-old female cat with nonregenerative anemia and thrombocytopenia was diagnosed with myelodysplastic syndromes (MDS), since peripheral blood and bone marrow (BM) examination revealed various dysplasias and a blast ratio of 19%. Chemotherapy with azacytidine (AZA; 70–35 mg/m², 3–5 days, three cycles) and treatment with prednisolone, antibiotics, and vitamin K2, and blood transfusion were performed. On day 106, blast cells and dysplasia had decreased in the BM, and the cat remained alive for at least 1,474 days. This report is the first on feline MDS treated with AZA, suggesting appropriate drug dosage, interval and effective combination should be investigated and the pharmacological and cell biological mechanisms needs to be elucidated in the future.  相似文献   

Changes in endogenous cytokinin-like substances and growth inhibitors in freesia corms during high-temperature treatment for breaking dormancy     

Masaharu Masuda  Tadashi Asahira 《Scientia Horticulturae》1978,8(4):371-382

High summer temperatures are required to break dormancy of freesia corms and cormels. Endogenous cytokinin levels were low in corms at the lifting-date, 25 May, but increased after 3 months storage at 30°C. Cytokinin levels in cormels increased gradually during summer storage at natural room temperature, reaching a maximum on 25 August when cormels had the capacity to sprout, then decreased to low levels on 25 September. Inhibitor levels in cormels decreased gradually during storage. A zeatin riboside-like compound, water-soluble cytokinins and an abscisic acid-like compound were present in freesia corms. Dormant corms, soaked for 18 h in 1000 mg/l benzyladenine solution, showed 100% sprouting. We conclude that high temperatures cause a decrease in inhibitors and an increase in cytokinins, which together result in the breaking of the dormant state of corms and cormels of freesia.  相似文献   

Tocopherol affinity for serum lipoproteins of Japanese flounder Paralichthys olivaceus during the reproduction period     

Masaharu Tokuda  Toshiyasu Yamaguchi  Kunihiro Wakui  Toshihiko Sato  Masao Ito and  Masaaki Takeuchi 《Fisheries Science》2000,66(4):619-624

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Genotypic Variation in Shoot Cadmium Concentration in Rice and Soybean in Soils with Different Levels of Cadmium Contamination   总被引：4，自引：0，他引：4  

Satoru Ishikawa  Noriharu Ae  Megumi Sugiyama  Masaharu Murakami  Tomohito Arao 《Soil Science and Plant Nutrition》2005,51(1):101-108

A pot experiment was conducted to investigate whether the shoot cadmium (Cd) concentration in 11 rice and 10 soybean cultivars varied among 4 soils with different levels of Cd contamination. Significant differences in shoot Cd concentration were found among rice or soybean cultivars grown in the 4 soils. The ranking of the rice cultivars for the shoot Cd concentration varied considerably among the soils. On the other hand, the soybean cultivars were ranked similarly in terms of shoot Cd concentration in the 4 soils. Significant and positive correlations were found between the Cd and Zn concentrations and between the Cd and Mn concentrations in the shoot of rice cultivars, when they were grown in 2 soils with relatively moderate levels of Cd contamination. The shoot Cd concentration in the soybean cultivars, however, was not correlated with the concentrations determined for any of the metals (Zn, Mn, Cu, and Fe) across the 4 soils. Significant and positive correlations between the concentrations of Cd in younger shoots and mature seeds were detected among the soybean cultivars in 2 soils used, unlike among the rice cultivars, indicating that it may be difficult to evaluate the genotypic variation in seed Cd concentration using relatively younger shoots in the case of rice. These results revealed that genotypic differences in shoot Cd concentration in rice or soybean are variable or invariable among soils, respectively.  相似文献