Molecular cloning of the canine c-Met/HGF receptor and its expression in normal and regenerated liver   总被引：4，自引：0，他引：4  

Neo S  Kansaku N  Furuichi M  Watanabe M  Hisamatsu S  Ohno K  Hisasue M  Tsuchiya R  Yamada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(5):525-529

The c-Met proto-oncogene is the receptor for hepatocyte growth factor (HGF), which is a member of the tyrosine kinase family. Activation of the HGF/c-Met signal pathway leads to cell proliferation, motility, regeneration, and morphogenesis. In this study, the complete nucleotide sequence of complementary DNA (cDNA) of canine c-Met was cloned, and its distribution was determined in tissues. The canine c-Met cDNA clone had an open reading frame of 4419 bp that encoded a putative polypeptide of 1383 amino acids. The c-Met mRNA was expressed in a variety of canine tissues including peripheral blood mononuclear cells (PBMC), bone marrow, liver, kidney, lung, stomach, uterus, testis, thymus, lymph node, small intestine, colon, adrenal gland, thyroid gland, heart, muscle, skin, pancreas, ovary, prostate, spleen, fat, cerebrum, and cerebellum. In addition, the c-Met mRNA expression in normal and regenerated liver was examined. The levels of the mRNA increased 2-fold in regenerated liver compared to that found in normal liver, indicating that c-Met is involved in various functions including remodeling of canine hepatocytes.  相似文献   

Iron-Enriched Bread with Karkade (Hibiscus sabdariffa) and Wheat Flour     

Machiko Hayashi  Masaharu Seguchi 《Cereal Chemistry》1998,75(5):686-689

Karkade (Hibiscus sabdariffa) was blended with wheat flour to make bread. When 0.5% Karkade was blended with wheat flour, maximum bread height and specific volume (cm³/g) were obtained (pH 4.8–5.0); these properties gradually deteriorated with increased Karkade. The pH of the bread crumb decreased with increased Karkade, reaching pH 3.35 when blended with 10% Karkade. The pH of Karkade alone was 2.5, which was adjusted to ≈5.0 by the addition of alkali just before blending with wheat flour and making bread. Control of the Karkade pH resulted in bread height and specific volume recovering to the original optimal levels. In addition, the deep reddish purple color (513 nm) of the bread crumb changed to a brownish color crumb. The Fe content was 0.14 mg of Fe/gram of dry crumb, or 6.22 mg of Fe/60 g of fresh bread when 5% Karkade was blended with wheat flour.  相似文献   

Springiness of Pancake and Its Relation to Binding of Prime Starch to Tailings in Stored Wheat Flour     

Masaharu Seguchi  Machiko Hayashi  Kazuko Kanenaga  Chiho Ishihara  Satoko Noguchi 《Cereal Chemistry》1998,75(1):37-42

Wheat flours were stored at room temperature (15–25°C), 40, 60, 80, and 100°C for various times. The baking performance of these flours was then evaluated in terms of the springiness of pancakes (recovery from crushing). Baking performance improved with increased storage time at each temperature. Brabender Amylograph tests of the flours indicated that the onset temperature in viscosity decreased with increased storage time at each temperature. When the flours were fractionated by acetic acid (pH 3.5) with mortar and pestle, recoveries of the water-solubles and gluten fractions were unchanged, but recoveries of prime starch and tailings fractions changed remarkably with increased storage time. On the other hand, those changes were not observed when flours were fractionated with a Waring blender. The binding of prime starch to tailings was correlated significantly with baking performance.  相似文献   

Effect of a Wheat Protein on pH-Dependent Water-Binding Capacity and Viscosity of Wheat Tailings Fractions     

Masaharu Seguchi  Machiko Hayashi  Hiroshi Matsumoto 《Cereal Chemistry》1997,74(4):384-388

Wheat flour was fractionated with acetic acid using a mortar and pestle method or a blender method. Higher pH-dependent water-binding capacity (WBC) and viscosity were obtained only in the tailings fraction. The higher pH-dependent WBC was rather stable at 5–37°C, however it decreased with salt addition. Pepsin or bromelain treatment stopped the pH-dependent changes in WBC and viscosity, which suggests that this characteristic of the tailings fraction is due to the presence of proteins. HPLC indicated that the M_r of the proteins associated with high WBC at low pH was >200 kDa.  相似文献   

Role of taurine deficiency in inducing green liver symptom and effect of dietary taurine supplementation in improving growth in juvenile red sea bream <Emphasis Type="Italic">Pagrus major</Emphasis> fed non-fishmeal diets based on soy protein concentrate     

Shusaku Takagi  Hisashi Murata  Takanobu Goto  Hideo Hatate  Makoto Endo  Hirofumi Yamashita  Hisashi Miyatake  Masaharu Ukawa 《Fisheries Science》2011,77(2):235-244

This study was conducted to investigate the mechanism of green liver symptom induction and the effect of dietary taurine supplementation on growth performance in juvenile red sea bream fed non-fishmeal diets based on soy protein concentrate (SPC). Juvenile fish (initial BW 72 g) were fed for 20 weeks on SPC diets supplemented with taurine at levels of 0, 1.0, and 2.0%. In the taurine-unsupplemented SPC diet group, specific growth rate (SGR) and feed conversion ratio (FCR) were significantly inferior (P < 0.001), and incidence of green liver was observed in 70% of fish. In this group, hepatopancreatic and plasma taurine concentrations were lowest (P < 0.05), hepatopancreatic content of bile pigments was highest (P < 0.05), and osmotic tolerance of erythrocytes was inferior (P < 0.05) among the dietary treatment groups. Serum osmolality of all treatment groups was at similar levels. These physiological abnormalities as well as SGR and FCR were improved by dietary taurine supplementation. These results indicate that the mechanism for induction of green liver symptom is bile pigment overproduction due to increased hemolysis because erythrocytes become osmotically fragile due to dietary taurine deficiency. Taurine supplementation of SPC diets is essential for maintaining normal physiological condition and growth performance in juvenile red sea bream.  相似文献   

Seasonal dynamics and yearly change in the abundance of metacercariae of <Emphasis Type="Italic">Parabucephalopsis parasiluri</Emphasis> (Trematoda: Bucephalidae) in the second intermediate host in the Uji–Yodo River,central Japan     

Misako Urabe  Kentaro Nakai  Daigo Nakamura  Masaharu Tanaka  Toshio Nakatsugawa  Kazuo Ogawa 《Fisheries Science》2009,75(1):63-70

An introduced pathogenic fish trematode, Parabucephalopsis parasiluri Wang, 1985 (Digenea: Bucephalidae), was studied to determine the seasonal fluctuation in its metacercariae abundance. The timing of the recruitment period of the metacercariae and the relationship between yearly fluctuation in metacercarial abundance and some environmental factors were also examined to develop an effective plan for disease control. Monthly changes in metacercarial abundance in the caudal fin of pale chub (Zacco platypus) and of the prevalence of the first intermediate host (golden mussel Limnoperna fortunei) suggest that P. parasiluri generally has an annual life cycle. The metacercarial abundance in chub increased when the water temperature fell below 7°C in winter, but the rapidity of the increase varied between 2007 and 2008. The fluctuations in the abundance of metacercariae in winter from 2001 to 2008 showed that mean flow rate and mean water level in January were negatively related to the infection level each year, and positively related to the mean water temperature. Based on these results, we propose a control plan for parabucephalopsiosis involving discharge control; that is, more than 50 m³/s (corresponding to 150 m³/s in the Yodo River) of water should be flushed from upstream dams for a period of at least one week starting on the day when the water temperature falls below 7°C.  相似文献   

云南稻-鸭共生模式效益的研究及综合评价（三）   总被引：15，自引：0，他引：15  

戴志明  杨华松张 曦 周晓智韩自鸿 余杨钱国平  张树清万田正治 中西良孝 魏红江 《中国农学通报》2004,20(4):265-265

通过不同放鸭只数的试验,研究了在中高产田单位面积放鸭的数量与产量构成因素和病虫草害发生的关系,分析了产量构成,病虫草害发生的情况,为稻－鸭共生模式技术的推广提供理论依据。  相似文献   

Efficacy of taurine supplementation for preventing green liver syndrome and improving growth performance in yearling red sea bream Pagrus major fed low-fishmeal diet   总被引：1，自引：0，他引：1  

Shusaku Takagi  Hisashi Murata  Takanobu Goto  Toshiaki Ichiki  Makoto Endo  Hideo Hatate  Terutoyo Yoshida  Tadashi Sakai  Hirofumi Yamashita  Masaharu Ukawa 《Fisheries Science》2006,72(6):1191-1199

ABSTRACT:   This study was performed to evaluate the efficacy of taurine supplementation for preventing green liver syndrome and improving growth performance in red sea bream Pagrus major fed a low-fishmeal (FM) diet. Yearling red sea bream were fed for 34 weeks on low-FM diets either supplemented with taurine, or without taurine, and the tissue taurine and bile pigment concentrations were measured. Compared to the fish fed the FM diet, fish fed the low-FM diet without taurine supplementation resulted in inferior feed performances and higher incidence of green liver related to the morphological transformation of the erythrocytes. In these fish, the hepatopancreatic taurine concentration was significantly lower and hepatopancreatic biliverdin concentration was high compared to the fish fed the FM diet. These parameters were markedly improved by taurine supplementation of the low-FM diet and were similar in levels to the fish fed the FM diet. These results indicate that green liver appearance and inferior feed performances of red sea bream fed the low-FM diet without taurine supplementation were caused by dietary taurine deficiency, and indicate the requirement of taurine supplementation to low-FM diets for red sea bream.  相似文献   

A microfluidic device for conducting gas-liquid-solid hydrogenation reactions     

Kobayashi J  Mori Y  Okamoto K  Akiyama R  Ueno M  Kitamori T  Kobayashi S 《Science (New York, N.Y.)》2004,304(5675):1305-1308

We have developed an efficient system for triphase reactions using a microchannel reactor. Using this system, we conducted hydrogenation reactions that proceeded smoothly to afford the desired products quantitatively within 2 minutes for a variety of substrates. The system could also be applied to deprotection reactions. We could achieve an effective interaction between hydrogen, substrates, and a palladium catalyst using extremely large interfacial areas and the short path required for molecular diffusion in the very narrow channel space. This concept could be extended to other multiphase reactions that use gas-phase reagents such as oxygen and carbon dioxide.  相似文献   

Anesthesia,fasting tolerance,and nutrient requirement of juvenile northern bluefin tuna     

Kenji?TakiiEmail author  Hidetsuyo?Hosokawa  Sadao?Shimeno  Masaharu?Ukawa  Akifumi?Kotani  Yojiro?Yamada 《Fisheries Science》2005,71(3):499-503

ABSTRACT:   The present study aimed to investigate a suitable anesthesia protocol for nutritional studies, fasting tolerance, and nutrient requirements for maintenance in juvenile northern bluefin tuna Thunnus orientalis weighing 0.8–0.9 g. The fish grew in a mass seedling production under completely controlled aquaculture. The solution of 0.2 mL 2-phenoxyethanol/L prepared by filtered seawater has been notably estimated as a anesthesia protocol for the juvenile, due to a shorter mean time necessary for anesthesia and recovery and higher survival rate at 24 h after recovery. All other anesthetics, 0.1 and 0.3 mL 2-phenoxyethanol/L, 0.05, 0.1 and 0.2 mL 4-allyl-2-methoxyphenol/L, and cool water temperature shocks at 16.8, 12.6 and 10.4°C, were inferior. In a 5-day tolerance test, the juvenile was fed sand lance Ammodytes personatus through the rearing period or fasted for 2, 3, 4 and 5 consecutive days. Survival rates were about 80 or 60, 50, 10 and 0%, respectively. From the relationships between days fasting and energetic and nutritional retentions, juvenile tuna require about 142.7 kJ, 5.46 g protein and 0.44 g fat/kg body weight a day for maintenance, indicating remarkably higher requirements than other aquaculture fish.  相似文献