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41.
Assessment of the response of the carbon cycle to climate change is important for predicting future climate, especially in northern high latitudes, where the warming may be highly pronounced. In this paper, three years of data measured by the eddy covariance method were used to verify the carbon dynamics of a black spruce forest in interior Alaska with the BIOME-BGC model. The model was modified and calibrated to the cold regions. The calibrated model simulation satisfactorily performed reproduction of the observed net ecosystem exchange (NEE), gross primary productivity (GPP), and ecosystem respiration (RE). The daily output of the model also correlated with field-based estimates of NEE (42%), GPP (89%), and RE (89%). The model simulation showed that the heterotrophic respiration occupied a substantial portion of total carbon exchange, and that inter-annual variation of the heterotrophic respiration determined the sink/source strength of carbon. According to our simulation, the black spruce forest in interior Alaska was a net carbon sink between 1949 and 2005, and the trend of the projected sink strength was not obvious in the recent climate warming. The model analysis suggested that the change in the seasonal weather pattern strongly affected the carbon balance. A potentially increased carbon sink due to future warming climate and elevated CO2 will be at least partly offset by a concurrent increase of VPD, since the response of the stomatal conductance to increased VPD could increase the soil moisture, and thus stimulate heterotrophic respiration, rather than net primary productivity. The results of this study should be useful to forecast the response of the carbon cycle to warming in black spruce forests of interior Alaska.  相似文献   
42.
At 114 ranches in the Hidaka District of Hokkaido, a total of 1,118 Thoroughbred foals that were born from January to June 2003 were examined at different times from May to September of the same year to ascertain the occurrence of clubfoot. Clubfoot was seen in a total of 179 foals (16%) at 89 ranches (78%). Also, 124 of the 179 foals (69.3%) had clubfoot of grade II or higher, and it was found that grade I was likely to be overlooked or rapidly advance. In the present study, the occurrence of clubfoot was higher than expected in the investigated region. Hence, it is necessary to establish accurate diagnostic techniques and clubfoot guidelines to minimize the impact.  相似文献   
43.
Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed.  相似文献   
44.
This study aimed to develop a water-extraction system which could produce the fresh water from the air in arid regions and which used renewable energies as the electric power source. In this paper, the experiments for water extraction from the air were carried out by using the novel multi-stage water-extraction device with Peltier deices for two cases of temperature and related humidity of the air. One was the case where the temperature and the related humidity of the air were constant, and the other was the case where they were simulated the variation of the temperature and related humidity of the air in a day of summer and spring in Loess Plateau, China. The effects of the temperature and related humidity of the atmospheric air and supply the electric power to Peltier devices on performance of water production of the device were investigated and reported.  相似文献   
45.
Abstract

A method for assaying the soil lipase activity is described. It involves the titrimetric estimation of the amount of lauric acid released by the lipase activity when the soil is incubated with Tween 20 in the presence of toluene at 30°C for 18 h under agitation. The method is simple and precise and incubation without agitation is also possible. The method has been applied to six different kinds of soils. The lipase activity in the cultivated soils ranged from 22.5 to 75.5 mmol min?1 g?1 of dried soil. The K m value for Tween 20 was 1.8 × 10?4 m. The optimum pH was approximately 7.5. The hydrolysis of liveen 20 in soil was inhibited by glycerol which was the essential moiety of glyceride. The inhibition by glycerol was found to be competitive. These results indicate that Tween 20 is a potential substrate for the assay of the glyceride hydrolytic activity in soils.  相似文献   
46.
Abstract

It is generally recognized that the nitrification activity in acid soils is very low. Indeed, nitrification in mineral soils has been found to be negligible at pH values below 5.0 (Dancer et al. 1973; Nyborg and Hoyt 1978). However, it was reported that autotrophic nitrification occurred in some tea soils at pH levels far below 5.0 (Walker and Wickramasinghe 1979; Hayatsu and Kosuge 1993). An acidophilic ammonia-oxidizing bacterium has been recently isolated from strongly acidic tea soils in Japan (Hayatsu 1993). On the other hand, fertilization has-been considered to be an important factor influencing nitrification in agricultural soils. For example, several studies have shown that the addition of ammoniacal fertilizer to soils can lead to the increase of the populations of Nitrosomonas (McLaren 1971; Ardakani et al. 1974). Liming of acidic soils also tends to stimulate the nitrification activity (Dancer et al. 1973; Nyborg and Hoyt 1978). Although nitrification has been studied in a wide variety of agricultural soils, there is little information available on nitrification in tea soils. The effect of fertilization on nitrification in tea soils is poorly documented.  相似文献   
47.
48.
Isolates of Diplocarpon mali, causal fungus of apple blotch, collected from four prefectures in Japan in 1997–1998 were tested for sensitivity to thiophanate-methyl. Results from mycelial growth tests showed that MIC values of the fungicide were 0.19 μg/ml against all isolates from Akita, Nagano and Saga prefectures but 100 or 200 μg/ml against all isolates from the Tokusa area in Yamaguchi prefecture. Detached apple leaves sprayed with the fungicide developed severe symptoms when inoculated with the isolate from Tokusa, but developed no symptoms with the isolate from Nagano. These results are the first confirmation of thiophanate-methyl-resistant strains in D. mali. Received 1 September 1999/ Accepted in revised form 15 October 1999  相似文献   
49.
Intramammary infusion of Bifidobacterium breve (B. breve)-induced somatic cell (SC) counts, chemiluminescent response (CL), lactoferrin (LF) concentrations and mastitis-causing pathogens from quarters with subclinical mastitis were measured to evaluate innate immune response of mammary glands in dairy cows at 3 to 4 weeks before drying off. SC counts in 7 quarters of 7 control cows and 5 quarters of 6 cows with mastitis increased markedly on day 1 and SC values in control cows were significantly (P<0.05) increased and returned to pre-infusion levels on day 5 after B. breve-infusion. CL values in both groups increased markedly on day 1 and then decreased after B. breve-infusion; however, CL values in cows with mastitis did not return to normal levels on day 5 and at postpartum. The CL values were highly correlated with their SC counts in milk from both groups. LF concentrations increased toward day 3 after B. breve-infusion and were higher in cows with mastitis. B. breve-infusion eliminated 16.6% (1/6) of pathogens from 6 quarters with chronic subclinical mastitis. B. breve-induced SC responses in quarters from 3 cows with mastitis showed characteristic patterns of recovery, persistent and new infections. B. breve-induced SC counts in quarters from the cows in the pre-drying off were lower (25.7–70.6%) than those of the cows in mid-lactation. The intrinsic innate immune response in cows on pre-drying off may be decreased and appears to be insufficient to eliminate pathogens from mammary gland in the pre-drying off.  相似文献   
50.
The effects of supplementation with cellulase, xylanase, pectinase, hemicellulase, glucanase, phytase and protease of microbial origin on digestibility of crude protein (CP) and dry matter (DM) of soybean meal in vitro were examined in the present study. Changes in viscosity during in vitro digestion were also examined as an index of carbohydrate digestibility. Hemicellulase and all enzyme combinations without protease showed significant improvement of CP digestibility. Cellulase, xylanase, phytase and glucanase tended to improve CP digestibility. Dry matter digestibility was improved significantly by pectinase and all enzyme combinations. Cellulase and phytase tended to improve DM digestibility. Crude protein and DM digestibilities were the highest when all the enzymes except protease were added. Protease, cellulase, pectinase, xylanase, glucanase and hemicellulase significantly reduced viscosity, while phytase had no effect on viscosity. Viscosity was increased unexpectedly when all the enzymes were added. These results strongly suggest that a combination of carbohydrases improves the CP and DM digestibility of soybean meal while protease inhibits the actions of these enzymes. The present study also suggests that viscosity is not always a good index of digestibility. Moreover, excluding the protease from commercial crude enzyme preparations may improve digestibility.  相似文献   
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