全文获取类型
收费全文 | 208篇 |
免费 | 8篇 |
国内免费 | 1篇 |
专业分类
林业 | 14篇 |
农学 | 7篇 |
61篇 | |
综合类 | 8篇 |
农作物 | 11篇 |
水产渔业 | 13篇 |
畜牧兽医 | 91篇 |
园艺 | 2篇 |
植物保护 | 10篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 4篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 27篇 |
2012年 | 22篇 |
2011年 | 16篇 |
2010年 | 4篇 |
2009年 | 10篇 |
2008年 | 15篇 |
2007年 | 14篇 |
2006年 | 13篇 |
2005年 | 11篇 |
2004年 | 7篇 |
2003年 | 7篇 |
2002年 | 3篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有217条查询结果,搜索用时 156 毫秒
11.
Kimiko HONSHO Michiko HIROSE Masanori HATORI Lubna YASMIN Haruna IZU Shogo MATOBA Sumie TOGAYACHI Hiroyuki MIYOSHI Tadashi SANKAI Atsuo OGURA Arata HONDA 《The Journal of reproduction and development》2015,61(1):13-19
Quality evaluation of pluripotent stem cells using appropriate animal models needs to be improved for human regenerative medicine. Previously, we demonstrated that although the in vitro neural differentiating capacity of rabbit induced pluripotent stem cells (iPSCs) can be mitigated by improving their baseline level of pluripotency, i.e., by converting them into the so-called “naïve-like” state, the effect after such conversion of rabbit embryonic stem cells (ESCs) remains to be elucidated. Here we found that naïve-like conversion enhanced the differences in innate in vitro differentiation capacity between ESCs and iPSCs. Naïve-like rabbit ESCs exhibited several features indicating pluripotency, including the capacity for teratoma formation. They differentiated into mature oligodendrocytes much more effectively (3.3–7.2 times) than naïve-like iPSCs. This suggests an inherent variation in differentiation potential in
vitro among PSC lines. When naïve-like ESCs were injected into preimplantation rabbit embryos, although they contributed efficiently to forming the inner cell mass of blastocysts, no chimeric pups were obtained. Thus, in vitro neural differentiation following naïve-like conversion is a promising option for determining the quality of PSCs without the need to demonstrate chimeric contribution. These results provide an opportunity to evaluate which pluripotent stem cells or treatments are best suited for therapeutic use. 相似文献
12.
Noritaka ADACHI Daisuke YAMAGUCHI Akiyuki WATANABE Narumi MIURA Seiji SUNAGA Hitoshi OISHI Michiko HASHIMOTO Takatsugu OISHI Masaki IWAMOTO Hirofumi HANADA Masanori KUBO Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105
The objective of this study was to examine the health and meat production of cloned sows
and their progenies in order to demonstrate the application of somatic cell cloning to the
pig industry. This study compared the growth, reproductive performance, carcass
characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies.
We measured their body weight, growth rate and feed conversion and performed a
pathological analysis of their anatomy to detect abnormalities. Three of the five cloned
pigs were used for a growth test. Cloned pigs grew normally and had characteristics
similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a
Landrace boar and used for a progeny test. F1 progenies had characteristics similar to
those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White
boar and used for the progeny test. F2 progenies grew normally. There were no biological
differences in growth, carcass characteristics and amino acid composition among cloned
sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies
showed normal reproductive performance. No specific abnormalities were observed by
pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs
had a normal karyotype. These results demonstrate that cloned female pigs and their
progenies have similar growth, reproductive performance and carcass quality
characteristics and that somatic cell cloning could be a useful technique for conserving
superior pig breeds in conventional meat production. 相似文献
13.
Yukinobu Nakamura Koshiro Migita Akihiro Okitani Masanori Matsuishi 《Animal Science Journal》2014,85(5):595-601
Water‐holding capacity (WHC) of heat‐induced pork gels was examined. The heat‐induced gels were obtained from meat homogenates prepared by adding nine volumes of 0.3–0.5 mol/L NaCl solutions containing 9–36 mmol/L disodium inosine‐5′‐monophosphate (IMP) or 9 mmol/L tetrapotassium pyrophosphate (KPP) to minced pork. IMP at 36 mmol/L enhanced the WHC to the same level as attained by KPP. Physical and sensory properties of heat‐induced gels were also examined. The heat‐induced gels were prepared from porcine meat homogenates containing 0.3 mol/L NaCl and 9–36 mmol/L IMP or 9 mmol/L KPP. IMP at 36 mmol/L enhanced the values of hardness, cohesiveness, gumminess and springiness, measured with a Tensipresser, and several organoleptic scores to the same level as the score attained by KPP. Thus, it is concluded that IMP is expected to be a practical substitute for pyrophosphates to improve the quality of sausages. 相似文献
14.
Yasumi Yagasaki Takashi Chishima Masanori Okazaki Du-Sik Jeon Jeong-Hwan Yoo Young-Kull Kim 《Water, air, and soil pollution》2001,130(1-4):1085-1090
The effect of acidic deposition on the soil under red pine forest in Chunchon, Korea was investigated. Precipitation, stream water, and soil solution chemistry were monitored at the watershed from 1997 to 1998. Acidity of the open-bulk precipitation was often neutralized by large amounts of ammonia (NH3) that might have originated from livestock farming and fertilization. Estimated elemental budget at the watershed showed a positive correlation between loss of base cations and proton (H+) production due to nitrogen transformation in soil (ΔH+ NT: ([NH4 +]in-[NH4 +]out)- ([NO3 ?]in-[NO3 ?]out)). When ΔH+ NT increased, concentrations of nitrate in soil solutions also increased. Consequently, pH values of soil solutions decreased, although ion exchange with base cations contributed to buffer reaction. Since acid buffering capacity of the red pine forest soil was small, it was concluded that the input of ammonium nitrogen enhanced nitrification in soil thus causing soil acidification represented by loss of base cations from the watershed. 相似文献
15.
Chihiro Kayo Seiji Hashimoto Atsunori Numata Masanori Hamada 《Journal of Wood Science》2011,57(3):234-240
We compared the greenhouse gas (GHG) emissions from a log pile (LP) to those from a sand compaction pile (SCP) and from cement
deep mixing (CDM) as measures against soil liquefaction, assuming that forest and waste management scenarios influence the
GHG (CO2, CH4, and N2O) balance of wood. We found little difference between the LP and SCP methods with respect to GHG emissions from fossil fuel
and limestone consumption. However, GHG emissions from the CDM method were seven times higher than emissions from the LP method.
In the GHG balance of wood, when the percentage of CH4 emissions from carbon in underground wood was lower than 3.3%, permanent storage in the log achieved greater reductions in
GHG emissions than using the waste log as fuel in place of coal or heavy oil. In order to obtain reductions in GHG emissions
by replacing SCPs or CDM with LPs, sustainable forest management with reforestation and prevention of CH4 emissions from the underground log are essential. Using reforestation, permanent storage of the log, no CH4 emission from the log, and using logging residues instead of coal, the LP can achieve reductions in GHG emissions of 121
tonnes of CO2 per 100 m2 of improvement area by replacing CDM. 相似文献
16.
The effect of soil freeze–thaw cycles on the denitrification potential was examined based on the C2H2 inhibition method. The gross N2O production curve of the soil sample (incubation with C2H2) showed minor changes between the freeze–thaw treatment and the unfrozen control. However, kinetics analysis revealed that
the initial production rate, an indicator of the population density of denitrifying communities, decreased (P = 0.043) and the specific growth rate constant, an indicator of the activity of denitrifying communities, increased (P = 0.039) as a result of the freeze–thaw cycles in five of six soil samples examined. The increase in the specific growth
rate constant suggested the stimulation of the activity of denitrifying communities that survived after the freeze–thaw cycles
and may explain the minor suppression on the gross N2O production in spite of decreasing the population density of denitrifying communities that was suggested by the initial production
rate. The net N2O production curve of the soil sample (incubation without C2H2) showed a remarkable change in one out of six soil samples, and in that one soil sample, N2O release to the atmosphere was largely stimulated (7.6 times) by the freeze–thaw cycles. However, the stimulation of the
N2O release by the freeze–thaw cycles was even observed in two other selected soil samples (4.6 and 1.8 times), suggesting that
an imbalance in the N2O-producing and N2O-reducing activities of denitrifying communities might complementally explain the N2O release stimulated by the freeze–thaw cycles. 相似文献
17.
18.
Khin Moe Kyaw Koki Toyota Masanori Okazaki Takashi Motobayashi Haruo Tanaka 《Biology and Fertility of Soils》2005,42(1):72-82
This paper focuses on N balance in a paddy field planted with whole crop rice (Oryza sativa cv. Kusahonami). The experiment was conducted with two treatments during two rice-growing seasons: one was fertilized with N (160 kg N ha–1; 16N plot) and the other unfertilized (0N plot); both plots were fertilized with P and K. The N input from precipitation was 15 and 12 kg N ha–1 in 2002 and 2003, respectively. The N input from irrigation water reached as much as 123 and 69 kg N ha–1 in 2002 and 2003, respectively. This was because irrigation water contained higher NO3– concentrations ranging from 4 to 8 mg N l–1. The N uptake by rice plants was the major output: 118 and 240 kg N ha–1 in the 0N and 16N plots in 2002 and 103 and 238 kg N ha–1 in 2003, respectively. N losses by leaching were 4.8–5.3 and 6.5–7.3 kg N ha–1 in 2002 and in 2003, respectively. Laboratory experiments were carried out to estimate the amounts of N2 fixation and denitrification. Amount of N2 fixation was 43 and 0 kg N ha–1 in the 0N and 16N plots, respectively. Denitrification potential was quite high in both the plots, and 90% of the N input through irrigation water was lost through denitrification. Collectively, the total N inputs were relatively large due to irrigation water contaminated with NO3–, but N outflow loading, expressed as leaching–(irrigation water + precipitation + fertilizer), showed large negative values, suggesting that the whole crop rice field might serve as a constructed wetland for decreasing N. 相似文献
19.
Masanori Yoshida Michihiro Onaka Toshio Fujita Minoru Nakajima 《Pesticide biochemistry and physiology》1979,10(3):313-321
Effects of various drugs including pesticides on the growth and respiration of cultured cells were evaluated comparatively using cell lines derived from mosquito ovary and subcutaneous mouse tissues. The concentration producing 50% inhibition of cell growth, I50 (M), was determined for each of 42 drugs. Inhibitors of respiration and nucleic acid and protein biosyntheses such as rotenone, piericidin A1, actinomycin D, and puromycin had very high pI50 values of approximately 8. Except for the compounds known to be uncouplers of oxidative phosphorylation, the drugs suppressed the respiration rate of the cultured cells to various degrees. The pI50 value (and the pEC150, 150% enhancement of the control, value for uncouplers) was determined for each compound. By examining the relation of pI50 (and pEC150) values between cell growth and respiration, the compounds could be classified into two groups according to their modes of inhibitory action against the cultured cells. One mode relates to the inhibition of energy synthesis and the other, perhaps, to interference with the biosynthesis of biomacromolecules. 相似文献
20.