Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1)     

Umeki S  Suzuki R  Shimojima M  Ema Y  Yanase T  Iwata H  Okuda M  Mizuno T 《Veterinary immunology and immunopathology》2011,142(1-2):119-125

Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.  相似文献   

Efficacy of en bloc thoracic duct ligation in combination with pericardiectomy by video-assisted thoracoscopic surgery for canine idiopathic chylothorax     

Hiroo Kanai DVM  Masaru Furuya DVM  PhD  Ken Hagiwara MD  Aya Nukaya DVM  Motoki Kondo DVM  PhD  Toshihide Aso DVM  Ayako Fujii DVM  Kazumi Sasai DVM  PhD 《Veterinary surgery : VS》2020,49(Z1):O102-O111

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Studies on the effect of humic acid on availability of phosphorus     

Azuma Okuda  Shiro Hori 《Soil Science and Plant Nutrition》2013,59(1):195-197

It is believed that some organic compounds form complexes with iron and aluminum and prevent the fixation of phosphate applied to soils.  相似文献   

Nitrogen-fixing microorganisms in paddy soils IV     

Azuma Okuda  Masuro Yamaguchi  Michiharu Kobayashi 《Soil Science and Plant Nutrition》2013,59(2):73-76

In 1949, GEST and KAMEN (1,2) reported that Photosynthetic bacteria can fix molecular nitrogen. LINDSTROM et al. (3,4) tested the nitrogen fixing ability of five species of photosynthetic bacteria under the following four conditions; 1) anaerobic light, 2) aerobic light, 3) aerobic dark, 4) anaerobic dark. As the result of the experiment, they certified that it Was only under the anaerobic light condition that the bacteria can fix molecular nitrogen.  相似文献   

Foliar absorption of nutrients: III     

Azuma Okuda  Yasuyuki Yamada 《Soil Science and Plant Nutrition》2013,59(2):14-18

In the last report¹⁾ the purity of radioactive potassium. which had been produced by ⁴¹K (d, p) ⁴²K nuclear reaction and separated from, radioactive anions by the use of ion exchange resin, was tested by means of its decay curve. By this test, it is difficult to distinguish ⁴²K from ²⁴Na contamination, because the half life of radioactive sodium (²⁴Na) is 15.06 hours²⁾ and it is very similar to that of radioactive potassium (⁴²K) which is 12.4 hours. When the nuclear reaction ²³Na (d, p) ²⁴Na occurs, the cross section of this reaction for deuterons is about 100 times that of potassium nuclear reaction. In this case the purity of radioactive potassium (⁴²K) could be confirmed by its Gamma-ray spectrum. According to the result, radioactive potassium of high purity was obtained. Potassium phosphate which was double-labeled by this radioactive potassium and imported radioactive phosphorus (³²P) was applied to leaves or roots of bean plants.  相似文献   

Foliar absorption of nutrients IV     

Azuma Okuda  Yasuyuki Yamada 《Soil Science and Plant Nutrition》2013,59(4):7-9

Active surface agents are often added to spray solutions to facilitate the uniform wetting and coveringg of the leaf surfaces^1),2). Some have reported that the addition of active surface agents increased the absorption of nutrients, but others have reported different results.  相似文献   

ABSTRACTS of the Articles Printed in Journal of the Science of Soil and Manure,Japan     

Tadashi Katayama  Michiharu Kobayashi  Azuma Okuda 《Soil Science and Plant Nutrition》2013,59(4):36-40

In the preceding paper (1), it was reported that even under aerobic shaking conditions, if R. capsulatus were mixed with Bacillus megaterium, the bacterial growth of both and nitrogen fixation were accelerated remarkably.  相似文献   

Nitrogen-fixing microoganisms in paddy soils XVI     

Tadashi Katayama  Michiharu Kobayashi  Azuma Okuda 《Soil Science and Plant Nutrition》2013,59(4):31-35

1. It was proved that under aerobic shaking conditions of the system of R. capsulatus mixed with Az. agilis or R. capsulatus mixed with B. megaterium, the bacterial growth of both and the nitrogen fixation were promoted markedly, comp:ired with the non-shaking culture. However, in the system of R. capsulatus mixed with B. subtilis under aerobic shaking conditions. such remarkable stimulative effects were not recognized altough some slow effects were recognized in the nonshaking culture.

2. It was also proved that under aerobic shaking conditions of the system of R. capsulatus mixed with Az. agilis or R. capsulatus mixed with B. megaterium the assimilative activity of carbon dioxide increased over 10 times more than in the separate pure culture.

From these results, it is conceivable that a very good environment was created for the internal metabolism of R. capsulatus in such mixing systems (R-Az and R-Bm).  相似文献   

Nucleic acids in the culture fluids of Rhodopseudomonas capsulatus and other heterotrophic bacteria     

Tadashi Katayama  Michiharu Kobayashi  Azuma Okuda 《Soil Science and Plant Nutrition》2013,59(4):101-106

In previous papers (1,2), it was reported that in cultures of R. capsulatus mixed with other heterotrophic bacteria nitrogen fixation is accelerated and there is some production of slime substances (3), exchange of pyruvic acid (4–6), amino acids, carbohydrates (7) and others in these mixed systems. There is also symbiotic association between R. capsulatus and other heterotrophic bacteria (Azotobacter) in nature (8).  相似文献   

Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA   总被引：1，自引：0，他引：1  

Nobuo Kobayashi  Daiki Mizuta  Akira Nakatsuka  Masaru Akabane 《Euphytica》2008,159(1-2):67-72

Inter-subgeneric hybrids were successfully obtained in reciprocal cross combinations between evergreen azaleas (Rhododendron nakaharae and its hybrids) and fragrant deciduous azaleas (R. arborescens and R. viscosum) for the purpose of fragrant evergreen azalea breeding. Nuclear and organelle DNA of these hybrids was investigated using PCR-RFLP markers. Viable hybrid seedlings have nuclear ribosomal DNA (nrDNA) inherited biparentally, mitochondrial DNA (mtDNA) from the seed parent, and chloroplast DNA (cpDNA) from the deciduous azalea, regardless of cross combination. These results suggest that the chloroplast genome from deciduous azaleas and the nuclear genome from evergreen azaleas are compatible in viable hybrid progenies.  相似文献